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Protein

Nuclear localization sequence-binding protein

Gene

NSR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-rRNA processing. Specifically binds nuclear localization sequences. Candidate for a receptor at the nucleus that may be involved in both RNA and protein transport. Binds telomeric sequences of the type (TG[1-3])n in vitro.

GO - Molecular functioni

  • nuclear localization sequence binding Source: SGD
  • nucleotide binding Source: InterPro
  • RNA binding Source: SGD
  • single-stranded telomeric DNA binding Source: SGD

GO - Biological processi

  • ribosomal small subunit assembly Source: SGD
  • rRNA processing Source: SGD
Complete GO annotation...

Keywords - Biological processi

rRNA processing, Stress response

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30859-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear localization sequence-binding protein
Alternative name(s):
p67
Gene namesi
Name:NSR1
Ordered Locus Names:YGR159C
ORF Names:G7001
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR159C.
SGDiS000003391. NSR1.

Subcellular locationi

  • Nucleusnucleolus 1 Publication

GO - Cellular componenti

  • nuclear envelope Source: SGD
  • nucleolus Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816901 – 414Nuclear localization sequence-binding proteinAdd BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei353Asymmetric dimethylarginine1 Publication1
Modified residuei362Asymmetric dimethylarginine1 Publication1
Modified residuei366Asymmetric dimethylarginine1 Publication1
Modified residuei375Asymmetric dimethylarginine1 Publication1
Modified residuei379Asymmetric dimethylarginine1 Publication1
Modified residuei382Asymmetric dimethylarginine1 Publication1

Post-translational modificationi

Methylated by HMT1, forming asymmetric dimethylarginines (DMA) within a domain referred to as an RGG box, made up of repeated Gly-Gly dipeptides interspersed with Arg and aromatic residues.1 Publication

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP27476.
PRIDEiP27476.

PTM databases

iPTMnetiP27476.

Expressioni

Inductioni

In response to low temperature (By cold-shock).

Interactioni

Protein-protein interaction databases

BioGridi33407. 75 interactors.
DIPiDIP-5537N.
IntActiP27476. 45 interactors.
MINTiMINT-543806.

Structurei

3D structure databases

ProteinModelPortaliP27476.
SMRiP27476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini168 – 246RRM 1PROSITE-ProRule annotationAdd BLAST79
Domaini267 – 345RRM 2PROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni353 – 384RGG-boxAdd BLAST32
Regioni366 – 384RNA-binding RGG-boxBy similarityAdd BLAST19

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 136Asp/Glu/Ser-richAdd BLAST108

Sequence similaritiesi

Belongs to the RRM GAR family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00840000129894.
HOGENOMiHOG000113864.
InParanoidiP27476.
KOiK11294.
OMAiDGRPINC.
OrthoDBiEOG092C5H62.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27476-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTTKVKGN KKEVKASKQA KEEKAKAVSS SSSESSSSSS SSSESESESE
60 70 80 90 100
SESESSSSSS SSDSESSSSS SSDSESEAET KKEESKDSSS SSSDSSSDEE
110 120 130 140 150
EEEEKEETKK EESKESSSSD SSSSSSSDSE SEKEESNDKK RKSEDAEEEE
160 170 180 190 200
DEESSNKKQK NEETEEPATI FVGRLSWSID DEWLKKEFEH IGGVIGARVI
210 220 230 240 250
YERGTDRSRG YGYVDFENKS YAEKAIQEMQ GKEIDGRPIN CDMSTSKPAG
260 270 280 290 300
NNDRAKKFGD TPSEPSDTLF LGNLSFNADR DAIFELFAKH GEVVSVRIPT
310 320 330 340 350
HPETEQPKGF GYVQFSNMED AKKALDALQG EYIDNRPVRL DFSSPRPNND
360 370 380 390 400
GGRGGSRGFG GRGGGRGGNR GFGGRGGARG GRGGFRPSGS GANTAPLGRS
410
RNTASFAGSK KTFD
Length:414
Mass (Da):44,535
Last modified:August 1, 1992 - v1
Checksum:i90DEEE7BBC20BC0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57185 Genomic DNA. Translation: CAA40472.1.
X85807 Genomic DNA. Translation: CAA59817.1.
Z72944 Genomic DNA. Translation: CAA97173.1.
Z72946 Genomic DNA. Translation: CAA97180.1.
BK006941 Genomic DNA. Translation: DAA08250.1.
PIRiA39205.
RefSeqiNP_011675.1. NM_001181288.1.

Genome annotation databases

EnsemblFungiiYGR159C; YGR159C; YGR159C.
GeneIDi853064.
KEGGisce:YGR159C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57185 Genomic DNA. Translation: CAA40472.1.
X85807 Genomic DNA. Translation: CAA59817.1.
Z72944 Genomic DNA. Translation: CAA97173.1.
Z72946 Genomic DNA. Translation: CAA97180.1.
BK006941 Genomic DNA. Translation: DAA08250.1.
PIRiA39205.
RefSeqiNP_011675.1. NM_001181288.1.

3D structure databases

ProteinModelPortaliP27476.
SMRiP27476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33407. 75 interactors.
DIPiDIP-5537N.
IntActiP27476. 45 interactors.
MINTiMINT-543806.

PTM databases

iPTMnetiP27476.

Proteomic databases

MaxQBiP27476.
PRIDEiP27476.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR159C; YGR159C; YGR159C.
GeneIDi853064.
KEGGisce:YGR159C.

Organism-specific databases

EuPathDBiFungiDB:YGR159C.
SGDiS000003391. NSR1.

Phylogenomic databases

GeneTreeiENSGT00840000129894.
HOGENOMiHOG000113864.
InParanoidiP27476.
KOiK11294.
OMAiDGRPINC.
OrthoDBiEOG092C5H62.

Enzyme and pathway databases

BioCyciYEAST:G3O-30859-MONOMER.

Miscellaneous databases

PROiP27476.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSR1_YEAST
AccessioniPrimary (citable) accession number: P27476
Secondary accession number(s): D6VUT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 77400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.