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Protein

Nuclear localization sequence-binding protein

Gene

NSR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-rRNA processing. Specifically binds nuclear localization sequences. Candidate for a receptor at the nucleus that may be involved in both RNA and protein transport. Binds telomeric sequences of the type (TG[1-3])n in vitro.

Miscellaneous

Present with 77400 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • nuclear localization sequence binding Source: SGD
  • RNA binding Source: SGD
  • single-stranded telomeric DNA binding Source: SGD

GO - Biological processi

  • ribosomal small subunit assembly Source: SGD
  • rRNA processing Source: SGD

Keywordsi

Molecular functionDNA-binding, RNA-binding
Biological processrRNA processing, Stress response

Enzyme and pathway databases

BioCyciYEAST:G3O-30859-MONOMER
ReactomeiR-SCE-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear localization sequence-binding protein
Alternative name(s):
p67
Gene namesi
Name:NSR1
Ordered Locus Names:YGR159C
ORF Names:G7001
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR159C
SGDiS000003391 NSR1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816901 – 414Nuclear localization sequence-binding proteinAdd BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei353Asymmetric dimethylarginine1 Publication1
Modified residuei362Asymmetric dimethylarginine1 Publication1
Modified residuei366Asymmetric dimethylarginine1 Publication1
Modified residuei375Asymmetric dimethylarginine1 Publication1
Modified residuei379Asymmetric dimethylarginine1 Publication1
Modified residuei382Asymmetric dimethylarginine1 Publication1

Post-translational modificationi

Methylated by HMT1, forming asymmetric dimethylarginines (DMA) within a domain referred to as an RGG box, made up of repeated Gly-Gly dipeptides interspersed with Arg and aromatic residues.1 Publication

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP27476
PaxDbiP27476
PRIDEiP27476

PTM databases

iPTMnetiP27476

Expressioni

Inductioni

In response to low temperature (By cold-shock).

Interactioni

Protein-protein interaction databases

BioGridi33407, 84 interactors
DIPiDIP-5537N
IntActiP27476, 52 interactors
MINTiP27476
STRINGi4932.YGR159C

Structurei

3D structure databases

ProteinModelPortaliP27476
SMRiP27476
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini168 – 246RRM 1PROSITE-ProRule annotationAdd BLAST79
Domaini267 – 345RRM 2PROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni353 – 384RGG-boxAdd BLAST32
Regioni366 – 384RNA-binding RGG-boxBy similarityAdd BLAST19

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 136Asp/Glu/Ser-richAdd BLAST108

Sequence similaritiesi

Belongs to the RRM GAR family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00900000141052
HOGENOMiHOG000113864
InParanoidiP27476
KOiK11294
OMAiREFEHIG
OrthoDBiEOG092C5H62

Family and domain databases

CDDicd12447 RRM1_gar2, 1 hit
cd12448 RRM2_gar2, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR034272 Gar2_RRM1
IPR034276 Gar2_RRM2
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits

Sequencei

Sequence statusi: Complete.

P27476-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTTKVKGN KKEVKASKQA KEEKAKAVSS SSSESSSSSS SSSESESESE
60 70 80 90 100
SESESSSSSS SSDSESSSSS SSDSESEAET KKEESKDSSS SSSDSSSDEE
110 120 130 140 150
EEEEKEETKK EESKESSSSD SSSSSSSDSE SEKEESNDKK RKSEDAEEEE
160 170 180 190 200
DEESSNKKQK NEETEEPATI FVGRLSWSID DEWLKKEFEH IGGVIGARVI
210 220 230 240 250
YERGTDRSRG YGYVDFENKS YAEKAIQEMQ GKEIDGRPIN CDMSTSKPAG
260 270 280 290 300
NNDRAKKFGD TPSEPSDTLF LGNLSFNADR DAIFELFAKH GEVVSVRIPT
310 320 330 340 350
HPETEQPKGF GYVQFSNMED AKKALDALQG EYIDNRPVRL DFSSPRPNND
360 370 380 390 400
GGRGGSRGFG GRGGGRGGNR GFGGRGGARG GRGGFRPSGS GANTAPLGRS
410
RNTASFAGSK KTFD
Length:414
Mass (Da):44,535
Last modified:August 1, 1992 - v1
Checksum:i90DEEE7BBC20BC0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57185 Genomic DNA Translation: CAA40472.1
X85807 Genomic DNA Translation: CAA59817.1
Z72944 Genomic DNA Translation: CAA97173.1
Z72946 Genomic DNA Translation: CAA97180.1
BK006941 Genomic DNA Translation: DAA08250.1
PIRiA39205
RefSeqiNP_011675.1, NM_001181288.1

Genome annotation databases

EnsemblFungiiYGR159C; YGR159C; YGR159C
GeneIDi853064
KEGGisce:YGR159C

Similar proteinsi

Entry informationi

Entry nameiNSR1_YEAST
AccessioniPrimary (citable) accession number: P27476
Secondary accession number(s): D6VUT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 23, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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