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P27470 (GTF2_STRDO) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucosyltransferase-I

Short name=GTF-I
EC=2.4.1.5
Alternative name(s):
Dextransucrase
Sucrose 6-glucosyltransferase
OrganismStreptococcus downei (Streptococcus sobrinus)
Taxonomic identifier1317 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length1592 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

Catalytic activity

Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).

Subcellular location

Secreted.

Miscellaneous

GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 70 family.

Contains 13 cell wall-binding repeats.

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseDental caries
   DomainRepeat
Signal
   Molecular functionGlycosyltransferase
Transferase
Gene Ontology (GO)
   Biological_processglucan biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondextransucrase activity

Inferred from electronic annotation. Source: UniProtKB-EC

glucosyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3838 Potential
Chain39 – 15921554Glucosyltransferase-I
PRO_0000021384

Regions

Repeat151 – 17020Cell wall-binding 1
Repeat172 – 19120Cell wall-binding 2
Repeat1083 – 110220Cell wall-binding 3
Repeat1103 – 112220Cell wall-binding 4
Repeat1167 – 118620Cell wall-binding 5
Repeat1211 – 123121Cell wall-binding 6
Repeat1232 – 125120Cell wall-binding 7
Repeat1276 – 129621Cell wall-binding 8
Repeat1297 – 131620Cell wall-binding 9
Repeat1411 – 143020Cell wall-binding 10
Repeat1455 – 147420Cell wall-binding 11
Repeat1523 – 154220Cell wall-binding 12
Repeat1567 – 158620Cell wall-binding 13
Region200 – 1044845Catalytic; approximate
Region1093 – 1592500Glucan-binding; approximate

Sequences

Sequence LengthMass (Da)Tools
P27470 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: BC0A66D079351ECF

FASTA1,592176,168
        10         20         30         40         50         60 
MEKNVRFKMH KVKKRWVTLS VASATMLASA LGASVASADT DTASDDSNQT VVTGDQTTNN 

        70         80         90        100        110        120 
QATDQTSIAA TATSEQSAST DAATDQASAA EQTQGTTAST DTAAQTTTNA NEAKWVPTEN 

       130        140        150        160        170        180 
ENQGFTDEML AEAKNVATAE SDSIPSDLAK MSNVKQVDGK YYYYDQDGNV KKNFAVSVGD 

       190        200        210        220        230        240 
KIYYFDETGA YKDTSKVDAD KSSSAVSQNA TIFAANNRAY STSAKNFEAV DNYLTADSWY 

       250        260        270        280        290        300 
RPKSILKDGK TWTESGKDDF RPLLMAWWPD TETKRNYVNY MNKVVGIDKT YTAETSQADL 

       310        320        330        340        350        360 
TAAAELVQAR IEQKITSENN TKWLREAISA FVKTQPQWNG ESEKPYDDHL QNGALLFDNQ 

       370        380        390        400        410        420 
TDLTPDTQSN YRLLNRTPTN QTGSLDSRFT YNPNDPLGGY DFLLANDVDN SNPVVQAEQL 

       430        440        450        460        470        480 
NWLHYLLNFG SIYANDADAN FDSIRVDAED NVDADQLQIS SDYLKAAYGI DKNNKNANNH 

       490        500        510        520        530        540 
VSIVEAWSDN DTPYLHDDGD NLMNMDNKFR LSMLWSLAKP TDVRSGLNPL IHNSLVDREV 

       550        560        570        580        590        600 
DDREVETVPS YSFARAHDSE VQDIIRDIIK AEINPNSFGY SFTQEEIDQA FKIYNEDLKK 

       610        620        630        640        650        660 
SDKKYTHYNV PLSYTLLLTN KGSIPRVYYG DMFTDDGQYM ANKTVNYDAI ESLLKARMKY 

       670        680        690        700        710        720 
VAGGQAMQNY QIGNGEILTS VRYGKGALKQ SDKGDATTRT SGVGVVMGNQ PNFSLDGKVV 

       730        740        750        760        770        780 
ALNMGAAHAN QEYRALMVST KDGVATYATD ADASKAGLVK RTDENGYLYF LNDDLKGVAN 

       790        800        810        820        830        840 
PQVSGFLQVW VPVGAADDQD IRVAASDTAS TDGKSLHQDA AMDSRVMFEG FSNFQSFATK 

       850        860        870        880        890        900 
EEEYTNVVIA NNVDKFVSWG ITDFEMAPQY VSSTDGQFQD SVIQNGYAFT DRYDLGMSKA 

       910        920        930        940        950        960 
NKYGTADQLV KAIKALHAKG LKVMADWVPD QMYTFPKQEV VTVTRTDKFG KPIAGSQINH 

       970        980        990       1000       1010       1020 
SLYVTDTKSS GDDYQAKYGG AFLDELKEKY PELFTKKQMS TGQAIDPSVK IKQWSAKYFN 

      1030       1040       1050       1060       1070       1080 
GSNILGRGAD YVLSDQVSNK YFNVASDTLF LPSSLLGKVV ESGIRYDGKG YIYNSSATGD 

      1090       1100       1110       1120       1130       1140 
QVKASFITEA GNLYYFGKDG YMVTGAQTIN GANYFFLENG TALRNTIYTD AQGNSHYYAN 

      1150       1160       1170       1180       1190       1200 
DGKRYENENG YQQFGNDWRY FKDGNMAVGL TTVDGNVQYF DKDGVQAKDK IIVTRDGKVR 

      1210       1220       1230       1240       1250       1260 
YFDQHNGNAV TNTFIADKTG HWYYLGKDGV AVTGAQTVGK QKLYFEANGQ QVKGDFVTSD 

      1270       1280       1290       1300       1310       1320 
EGKLYFYDVD SGDMWTDTFI EDKAGNWFYL GKDGAAVTGA QTIRGQKLYF KANGQQVKGD 

      1330       1340       1350       1360       1370       1380 
IVKGTDGKIR YYDAKSGEQV FNKTVKAADG KTYVIGNDGV AVDPSVVKGQ TFKDASGALR 

      1390       1400       1410       1420       1430       1440 
FYNLKGQLVT GSGWYETANH DWVYIQSGKA LTGEQTINGQ HLYFKKDGHQ VKGQLVTGTD 

      1450       1460       1470       1480       1490       1500 
GKVRYYDANS GDQAFNKSVT VNGKTYYFGN DGTAQTAGNP KGQTFKDGSD IRFYSMEGQL 

      1510       1520       1530       1540       1550       1560 
VTGSGWYSNA QGQWLYVKNG KVLTGLQTVG SQRVYFDENG IQAKGKAVRT SDGKIRYFDE 

      1570       1580       1590 
NSGSMITNQW KEVNGRYYYF GNDGARIYRG WN 

« Hide

References

[1]"Peptide sequences for sucrose splitting and glucan binding within Streptococcus sobrinus glucosyltransferase (water-insoluble glucan synthetase)."
Abo H., Matsumura T., Kodama T., Ohta H., Fukui K., Kato K., Kagawa H.
J. Bacteriol. 173:989-996(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 6715 / Serotype G.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D90213 Genomic DNA. Translation: BAA14241.1.

3D structure databases

ProteinModelPortalP27470.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH70. Glycoside Hydrolase Family 70.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 4 hits.
InterProIPR018337. Cell_wall/Cho-bd_repeat.
IPR027636. Glucan-bd_rpt.
IPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR022263. KxYKxGKxW.
[Graphical view]
PfamPF01473. CW_binding_1. 5 hits.
PF02324. Glyco_hydro_70. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 4 hits.
TIGRFAMsTIGR04035. glucan_65_rpt. 5 hits.
TIGR03715. KxYKxGKxW. 1 hit.
PROSITEPS51170. CW. 13 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGTF2_STRDO
AccessionPrimary (citable) accession number: P27470
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 16, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries