P27467 (WNT3A_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 119.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Protein Wnt-3a | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) [Reference proteome] | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 352 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. |
| Subunit structure | Homooligomer; disulfide-linked, leading to inactivation. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6 By similarity. Interacts with PORCN. Ref.2 Ref.4 |
| Subcellular location | |
| Tissue specificity | Dorsal portion of the neural tube (developing roof plate), and mesenchyme tissue surrounding the umbilical veins. |
| Post-translational modification | Palmitoylation at Ser-209 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-77. Palmitoylation is required for proper trafficking to cell surface, vacuolar acidification is critical to release palmitoylated WNT3A from WLS in secretory vesicles By similarity. Ref.3 Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT3A. |
| Sequence similarities | Belongs to the Wnt family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| TRABD2B | A6NFA1 | 2 | EBI-2899665,EBI-6257471 | From a different organism. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 18 | 18 | Potential | ||||||
| Chain | 19 – 352 | 334 | Protein Wnt-3a | PRO_0000041419 | |||||
Sites | |||||||||
| Site | 26 – 27 | 2 | Cleavage; by TIKI1 and TIKI2 | ||||||
Amino acid modifications | |||||||||
| Lipidation | 77 | 1 | S-palmitoyl cysteine Ref.3 | ||||||
| Lipidation | 209 | 1 | O-palmitoyl serine; by PORCN By similarity | ||||||
| Glycosylation | 87 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 298 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Mutagenesis | 25 – 26 | 2 | SL → DD: Partially resistant to proteolysis by TIKI1 and TIKI2. Completely resistant to proteolysis by TIKI1 and TIKI2; when associated with 33-D-D-34. | ||||||
| Mutagenesis | 33 – 34 | 2 | SS → DD: Completely resistant to proteolysis by TIKI1 and TIKI2; when associated with 25-D-D-26. | ||||||
| Mutagenesis | 77 | 1 | C → A: Forms oxidized oligomers regardless of TIKI2. Ref.4 | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "Expression of two members of the Wnt family during mouse development -- restricted temporal and spatial patterns in the developing neural tube." Roelink H., Nusse R. Genes Dev. 5:381-388(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo. |
| [2] | "The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family." Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T. Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PORCN. |
| [3] | "Wnt proteins are lipid-modified and can act as stem cell growth factors." Willert K., Brown J.D., Danenberg E., Duncan A.W., Weissman I.L., Reya T., Yates J.R. III, Nusse R. Nature 423:448-452(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PALMITOYLATION AT CYS-77. |
| [4] | "Tiki1 is required for head formation via Wnt cleavage-oxidation and inactivation." Zhang X., Abreu J.G., Yokota C., Macdonald B.T., Singh S., Coburn K.L., Cheong S.M., Zhang M.M., Ye Q.Z., Hang H.C., Steen H., He X. Cell 149:1565-1577(2012) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEOLYTIC PROCESSING BY TIKI1 AND TIKI2, DISULFIDE BONDS, SUBUNIT, MUTAGENESIS OF 25-SER-LEU-26; 33-SER-SER-34 AND CYS-77. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X56842 mRNA. Translation: CAA40173.1. |
| IPI | IPI00116906. |
| PIR | A39532. |
| RefSeq | NP_033548.1. NM_009522.2. |
| UniGene | Mm.1367. |
3D structure databases | |
| ProteinModelPortal | P27467. |
| SMR | P27467. Positions 57-351. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-55954N. |
| IntAct | P27467. 2 interactions. |
| STRING | 10090.ENSMUSP00000010044. |
PTM databases | |
| PhosphoSite | P27467. |
Proteomic databases | |
| PRIDE | P27467. |
Protocols and materials databases | |
| DNASU | 22416. |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000010044; ENSMUSP00000010044; ENSMUSG00000009900. |
| GeneID | 22416. |
| KEGG | mmu:22416. |
| UCSC | uc007jdp.2. mouse. |
Organism-specific databases | |
| CTD | 89780. |
| MGI | MGI:98956. Wnt3a. |
Phylogenomic databases | |
| eggNOG | NOG284879. |
| GeneTree | ENSGT00690000101771. |
| HOGENOM | HOG000039529. |
| HOVERGEN | HBG001595. |
| InParanoid | P27467. |
| KO | K00312. |
| OMA | QAIASHM. |
| OrthoDB | EOG4XD3R9. |
Gene expression databases | |
| Bgee | P27467. |
| CleanEx | MM_WNT3A. |
| Genevestigator | P27467. |
| GermOnline | ENSMUSG00000009900. Mus musculus. |
Family and domain databases | |
| InterPro | IPR005817. Wnt. IPR009141. Wnt3. IPR018161. Wnt_CS. [Graphical view] |
| PANTHER | PTHR12027. PTHR12027. 1 hit. PTHR12027:SF18. PTHR12027:SF18. 1 hit. |
| Pfam | PF00110. wnt. 1 hit. [Graphical view] |
| PRINTS | PR01843. WNT3PROTEIN. PR01349. WNTPROTEIN. |
| SMART | SM00097. WNT1. 1 hit. [Graphical view] |
| PROSITE | PS00246. WNT1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| ChEMBL | CHEMBL5617. |
| NextBio | 302833. |
| SOURCE | Search... |
Entry information
| Entry name | WNT3A_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P27467 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |