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P27466

- KCC1_YEAST

UniProt

P27466 - KCC1_YEAST

Protein

Calcium/calmodulin-dependent protein kinase I

Gene

CMK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Important in cellular regulation.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei66 – 661ATPPROSITE-ProRule annotation
    Active sitei161 – 1611Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi43 – 519ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calmodulin-dependent protein kinase activity Source: SGD

    GO - Biological processi

    1. protein phosphorylation Source: SGD
    2. signal transduction Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30467-MONOMER.
    BRENDAi2.7.11.17. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium/calmodulin-dependent protein kinase I (EC:2.7.11.17)
    Gene namesi
    Name:CMK1
    Ordered Locus Names:YFR014C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFR014c.
    SGDiS000001910. CMK1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 446446Calcium/calmodulin-dependent protein kinase IPRO_0000086090Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei417 – 4171Phosphoserine2 Publications
    Modified residuei418 – 4181Phosphoserine1 Publication
    Modified residuei420 – 4201Phosphoserine2 Publications
    Modified residuei429 – 4291Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP27466.
    PaxDbiP27466.
    PeptideAtlasiP27466.

    Expressioni

    Gene expression databases

    GenevestigatoriP27466.

    Interactioni

    Subunit structurei

    Multimeric.

    Protein-protein interaction databases

    BioGridi31166. 234 interactions.
    DIPiDIP-2305N.
    IntActiP27466. 33 interactions.
    MINTiMINT-687892.
    STRINGi4932.YFR014C.

    Structurei

    3D structure databases

    ProteinModelPortaliP27466.
    SMRiP27466. Positions 14-323.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 299263Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni312 – 32312Calmodulin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117487.
    HOGENOMiHOG000233016.
    KOiK08794.
    OMAiFESKDKY.
    OrthoDBiEOG793BK1.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27466-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDKVSEKES SPKQTEEDSE GKMAHVQPAS YVNKKKYVFG KTLGAGTFGV    50
    VRQAKNTETG EDVAVKILIK KALKGNKVQL EALYDELDIL QRLHHPNIVA 100
    FKDWFESKDK FYIITQLAKG GELFDRILKK GKFTEEDAVR ILVEILSAVK 150
    YMHSQNIVHR DLKPENLLYI DKSDESPLVV ADFGIAKRLK SDEELLYKPA 200
    GSLGYVAPEV LTQDGHGKPC DIWSIGVITY TLLCGYSAFR AERVQDFLDE 250
    CTTGEYPVKF HRPYWDSVSN KAKQFILKAL NLDPSKRPTA AELLEDPWII 300
    CTELKTHNLL PGLKEGLDAR QKFRNSVERV RLNMKIQKLR DLYLEQTESD 350
    SDFDEGSQAN GSVPPLKATD TSQLSKKLSE EEQSKLKSEL TSKAFAQLVN 400
    TVLAEKEKFL NINRVCSSDS DLPGSDIKSL DEAKEKPEGK DTKTEE 446
    Length:446
    Mass (Da):50,296
    Last modified:November 1, 1995 - v2
    Checksum:i586C31CED6311555
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 542QA → VR in CAA40928. (PubMed:2026147)Curated
    Sequence conflicti68 – 681L → V in CAA40928. (PubMed:2026147)Curated
    Sequence conflicti188 – 1881R → T in CAA40928. (PubMed:2026147)Curated
    Sequence conflicti199 – 2024PAGS → AGTA in CAA40928. (PubMed:2026147)Curated
    Sequence conflicti237 – 2437SAFRAER → ATIDRK in CAA40928. (PubMed:2026147)Curated
    Sequence conflicti271 – 2711K → Q in CAA40928. (PubMed:2026147)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57782 Genomic DNA. Translation: CAA40928.1.
    D90375 Genomic DNA. Translation: BAA14383.1.
    D50617 Genomic DNA. Translation: BAA09253.1.
    BK006940 Genomic DNA. Translation: DAA12455.1.
    PIRiA40896.
    RefSeqiNP_116669.1. NM_001179979.2.

    Genome annotation databases

    EnsemblFungiiYFR014C; YFR014C; YFR014C.
    GeneIDi850568.
    KEGGisce:YFR014C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57782 Genomic DNA. Translation: CAA40928.1 .
    D90375 Genomic DNA. Translation: BAA14383.1 .
    D50617 Genomic DNA. Translation: BAA09253.1 .
    BK006940 Genomic DNA. Translation: DAA12455.1 .
    PIRi A40896.
    RefSeqi NP_116669.1. NM_001179979.2.

    3D structure databases

    ProteinModelPortali P27466.
    SMRi P27466. Positions 14-323.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31166. 234 interactions.
    DIPi DIP-2305N.
    IntActi P27466. 33 interactions.
    MINTi MINT-687892.
    STRINGi 4932.YFR014C.

    Proteomic databases

    MaxQBi P27466.
    PaxDbi P27466.
    PeptideAtlasi P27466.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFR014C ; YFR014C ; YFR014C .
    GeneIDi 850568.
    KEGGi sce:YFR014C.

    Organism-specific databases

    CYGDi YFR014c.
    SGDi S000001910. CMK1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117487.
    HOGENOMi HOG000233016.
    KOi K08794.
    OMAi FESKDKY.
    OrthoDBi EOG793BK1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30467-MONOMER.
    BRENDAi 2.7.11.17. 984.

    Miscellaneous databases

    NextBioi 966378.
    PROi P27466.

    Gene expression databases

    Genevestigatori P27466.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Multiple Ca2+/calmodulin-dependent protein kinase genes in a unicellular eukaryote."
      Pausch M.H., Kaim D., Kunisawa R., Admon A., Thorner J.
      EMBO J. 10:1511-1522(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Two yeast genes encoding calmodulin-dependent protein kinases. Isolation, sequencing and bacterial expressions of CMK1 and CMK2."
      Ohya Y., Kawasaki H., Suzuki K., Londesborough J., Anraku Y.
      J. Biol. Chem. 266:12784-12794(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417; SER-420 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417; SER-418; SER-420 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKCC1_YEAST
    AccessioniPrimary (citable) accession number: P27466
    Secondary accession number(s): D6VTP5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1520 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3