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Protein

Calcium/calmodulin-dependent protein kinase I

Gene

CMK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important in cellular regulation.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei66ATPPROSITE-ProRule annotation1
Active sitei161Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi43 – 51ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin-dependent protein kinase activity Source: SGD

GO - Biological processi

  • protein phosphorylation Source: SGD
  • signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30467-MONOMER.
BRENDAi2.7.11.17. 984.
ReactomeiR-SCE-111932. CaMK IV-mediated phosphorylation of CREB.
R-SCE-3371571. HSF1-dependent transactivation.
R-SCE-4086398. Ca2+ pathway.
R-SCE-442729. CREB phosphorylation through the activation of CaMKII.
R-SCE-442745. Activation of CaMK IV.
R-SCE-5578775. Ion homeostasis.
R-SCE-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase I (EC:2.7.11.17)
Gene namesi
Name:CMK1
Ordered Locus Names:YFR014C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR014C.
SGDiS000001910. CMK1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860901 – 446Calcium/calmodulin-dependent protein kinase IAdd BLAST446

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei343PhosphotyrosineBy similarity1
Modified residuei357PhosphoserineBy similarity1
Modified residuei417PhosphoserineCombined sources1
Modified residuei418PhosphoserineCombined sources1
Modified residuei420PhosphoserineCombined sources1
Modified residuei429PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27466.
PRIDEiP27466.

PTM databases

iPTMnetiP27466.

Interactioni

Subunit structurei

Multimeric.

Protein-protein interaction databases

BioGridi31166. 234 interactors.
DIPiDIP-2305N.
IntActiP27466. 33 interactors.
MINTiMINT-687892.

Structurei

3D structure databases

ProteinModelPortaliP27466.
SMRiP27466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 299Protein kinasePROSITE-ProRule annotationAdd BLAST263

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni312 – 323Calmodulin-bindingBy similarityAdd BLAST12

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
InParanoidiP27466.
KOiK08794.
OMAiFESKDKY.
OrthoDBiEOG092C0QJU.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDKVSEKES SPKQTEEDSE GKMAHVQPAS YVNKKKYVFG KTLGAGTFGV
60 70 80 90 100
VRQAKNTETG EDVAVKILIK KALKGNKVQL EALYDELDIL QRLHHPNIVA
110 120 130 140 150
FKDWFESKDK FYIITQLAKG GELFDRILKK GKFTEEDAVR ILVEILSAVK
160 170 180 190 200
YMHSQNIVHR DLKPENLLYI DKSDESPLVV ADFGIAKRLK SDEELLYKPA
210 220 230 240 250
GSLGYVAPEV LTQDGHGKPC DIWSIGVITY TLLCGYSAFR AERVQDFLDE
260 270 280 290 300
CTTGEYPVKF HRPYWDSVSN KAKQFILKAL NLDPSKRPTA AELLEDPWII
310 320 330 340 350
CTELKTHNLL PGLKEGLDAR QKFRNSVERV RLNMKIQKLR DLYLEQTESD
360 370 380 390 400
SDFDEGSQAN GSVPPLKATD TSQLSKKLSE EEQSKLKSEL TSKAFAQLVN
410 420 430 440
TVLAEKEKFL NINRVCSSDS DLPGSDIKSL DEAKEKPEGK DTKTEE
Length:446
Mass (Da):50,296
Last modified:November 1, 1995 - v2
Checksum:i586C31CED6311555
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53 – 54QA → VR in CAA40928 (PubMed:2026147).Curated2
Sequence conflicti68L → V in CAA40928 (PubMed:2026147).Curated1
Sequence conflicti188R → T in CAA40928 (PubMed:2026147).Curated1
Sequence conflicti199 – 202PAGS → AGTA in CAA40928 (PubMed:2026147).Curated4
Sequence conflicti237 – 243SAFRAER → ATIDRK in CAA40928 (PubMed:2026147).Curated7
Sequence conflicti271K → Q in CAA40928 (PubMed:2026147).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57782 Genomic DNA. Translation: CAA40928.1.
D90375 Genomic DNA. Translation: BAA14383.1.
D50617 Genomic DNA. Translation: BAA09253.1.
BK006940 Genomic DNA. Translation: DAA12455.1.
PIRiA40896.
RefSeqiNP_116669.1. NM_001179979.2.

Genome annotation databases

EnsemblFungiiBAA09253; BAA09253; BAA09253.
YFR014C; YFR014C; YFR014C.
GeneIDi850568.
KEGGisce:YFR014C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57782 Genomic DNA. Translation: CAA40928.1.
D90375 Genomic DNA. Translation: BAA14383.1.
D50617 Genomic DNA. Translation: BAA09253.1.
BK006940 Genomic DNA. Translation: DAA12455.1.
PIRiA40896.
RefSeqiNP_116669.1. NM_001179979.2.

3D structure databases

ProteinModelPortaliP27466.
SMRiP27466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31166. 234 interactors.
DIPiDIP-2305N.
IntActiP27466. 33 interactors.
MINTiMINT-687892.

PTM databases

iPTMnetiP27466.

Proteomic databases

MaxQBiP27466.
PRIDEiP27466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09253; BAA09253; BAA09253.
YFR014C; YFR014C; YFR014C.
GeneIDi850568.
KEGGisce:YFR014C.

Organism-specific databases

EuPathDBiFungiDB:YFR014C.
SGDiS000001910. CMK1.

Phylogenomic databases

GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
InParanoidiP27466.
KOiK08794.
OMAiFESKDKY.
OrthoDBiEOG092C0QJU.

Enzyme and pathway databases

BioCyciYEAST:G3O-30467-MONOMER.
BRENDAi2.7.11.17. 984.
ReactomeiR-SCE-111932. CaMK IV-mediated phosphorylation of CREB.
R-SCE-3371571. HSF1-dependent transactivation.
R-SCE-4086398. Ca2+ pathway.
R-SCE-442729. CREB phosphorylation through the activation of CaMKII.
R-SCE-442745. Activation of CaMK IV.
R-SCE-5578775. Ion homeostasis.
R-SCE-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiP27466.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCC1_YEAST
AccessioniPrimary (citable) accession number: P27466
Secondary accession number(s): D6VTP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.