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P27466

- KCC1_YEAST

UniProt

P27466 - KCC1_YEAST

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Protein

Calcium/calmodulin-dependent protein kinase I

Gene
CMK1, YFR014C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Important in cellular regulation.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei66 – 661ATP By similarity
Active sitei161 – 1611Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 519ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calmodulin-dependent protein kinase activity Source: SGD

GO - Biological processi

  1. protein phosphorylation Source: SGD
  2. signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30467-MONOMER.
BRENDAi2.7.11.17. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase I (EC:2.7.11.17)
Gene namesi
Name:CMK1
Ordered Locus Names:YFR014C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFR014c.
SGDiS000001910. CMK1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Calcium/calmodulin-dependent protein kinase IPRO_0000086090Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei417 – 4171Phosphoserine2 Publications
Modified residuei418 – 4181Phosphoserine1 Publication
Modified residuei420 – 4201Phosphoserine2 Publications
Modified residuei429 – 4291Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27466.
PaxDbiP27466.
PeptideAtlasiP27466.

Expressioni

Gene expression databases

GenevestigatoriP27466.

Interactioni

Subunit structurei

Multimeric.

Protein-protein interaction databases

BioGridi31166. 234 interactions.
DIPiDIP-2305N.
IntActiP27466. 33 interactions.
MINTiMINT-687892.
STRINGi4932.YFR014C.

Structurei

3D structure databases

ProteinModelPortaliP27466.
SMRiP27466. Positions 14-323.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 299263Protein kinaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni312 – 32312Calmodulin-binding By similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117487.
HOGENOMiHOG000233016.
KOiK08794.
OMAiFESKDKY.
OrthoDBiEOG793BK1.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27466-1 [UniParc]FASTAAdd to Basket

« Hide

MDDKVSEKES SPKQTEEDSE GKMAHVQPAS YVNKKKYVFG KTLGAGTFGV    50
VRQAKNTETG EDVAVKILIK KALKGNKVQL EALYDELDIL QRLHHPNIVA 100
FKDWFESKDK FYIITQLAKG GELFDRILKK GKFTEEDAVR ILVEILSAVK 150
YMHSQNIVHR DLKPENLLYI DKSDESPLVV ADFGIAKRLK SDEELLYKPA 200
GSLGYVAPEV LTQDGHGKPC DIWSIGVITY TLLCGYSAFR AERVQDFLDE 250
CTTGEYPVKF HRPYWDSVSN KAKQFILKAL NLDPSKRPTA AELLEDPWII 300
CTELKTHNLL PGLKEGLDAR QKFRNSVERV RLNMKIQKLR DLYLEQTESD 350
SDFDEGSQAN GSVPPLKATD TSQLSKKLSE EEQSKLKSEL TSKAFAQLVN 400
TVLAEKEKFL NINRVCSSDS DLPGSDIKSL DEAKEKPEGK DTKTEE 446
Length:446
Mass (Da):50,296
Last modified:November 1, 1995 - v2
Checksum:i586C31CED6311555
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 542QA → VR in CAA40928. 1 Publication
Sequence conflicti68 – 681L → V in CAA40928. 1 Publication
Sequence conflicti188 – 1881R → T in CAA40928. 1 Publication
Sequence conflicti199 – 2024PAGS → AGTA in CAA40928. 1 Publication
Sequence conflicti237 – 2437SAFRAER → ATIDRK in CAA40928. 1 Publication
Sequence conflicti271 – 2711K → Q in CAA40928. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X57782 Genomic DNA. Translation: CAA40928.1.
D90375 Genomic DNA. Translation: BAA14383.1.
D50617 Genomic DNA. Translation: BAA09253.1.
BK006940 Genomic DNA. Translation: DAA12455.1.
PIRiA40896.
RefSeqiNP_116669.1. NM_001179979.2.

Genome annotation databases

EnsemblFungiiYFR014C; YFR014C; YFR014C.
GeneIDi850568.
KEGGisce:YFR014C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X57782 Genomic DNA. Translation: CAA40928.1 .
D90375 Genomic DNA. Translation: BAA14383.1 .
D50617 Genomic DNA. Translation: BAA09253.1 .
BK006940 Genomic DNA. Translation: DAA12455.1 .
PIRi A40896.
RefSeqi NP_116669.1. NM_001179979.2.

3D structure databases

ProteinModelPortali P27466.
SMRi P27466. Positions 14-323.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31166. 234 interactions.
DIPi DIP-2305N.
IntActi P27466. 33 interactions.
MINTi MINT-687892.
STRINGi 4932.YFR014C.

Proteomic databases

MaxQBi P27466.
PaxDbi P27466.
PeptideAtlasi P27466.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFR014C ; YFR014C ; YFR014C .
GeneIDi 850568.
KEGGi sce:YFR014C.

Organism-specific databases

CYGDi YFR014c.
SGDi S000001910. CMK1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117487.
HOGENOMi HOG000233016.
KOi K08794.
OMAi FESKDKY.
OrthoDBi EOG793BK1.

Enzyme and pathway databases

BioCyci YEAST:G3O-30467-MONOMER.
BRENDAi 2.7.11.17. 984.

Miscellaneous databases

NextBioi 966378.
PROi P27466.

Gene expression databases

Genevestigatori P27466.

Family and domain databases

InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple Ca2+/calmodulin-dependent protein kinase genes in a unicellular eukaryote."
    Pausch M.H., Kaim D., Kunisawa R., Admon A., Thorner J.
    EMBO J. 10:1511-1522(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Two yeast genes encoding calmodulin-dependent protein kinases. Isolation, sequencing and bacterial expressions of CMK1 and CMK2."
    Ohya Y., Kawasaki H., Suzuki K., Londesborough J., Anraku Y.
    J. Biol. Chem. 266:12784-12794(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417; SER-420 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417; SER-418; SER-420 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKCC1_YEAST
AccessioniPrimary (citable) accession number: P27466
Secondary accession number(s): D6VTP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

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