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Protein

Calcium/calmodulin-dependent protein kinase I

Gene

CMK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important in cellular regulation.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei66 – 661ATPPROSITE-ProRule annotation
Active sitei161 – 1611Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 519ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin-dependent protein kinase activity Source: SGD

GO - Biological processi

  • protein phosphorylation Source: SGD
  • signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30467-MONOMER.
BRENDAi2.7.11.17. 984.
ReactomeiREACT_336144. CREB phosphorylation through the activation of CaMKII.
REACT_353242. HSF1-dependent transactivation.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase I (EC:2.7.11.17)
Gene namesi
Name:CMK1
Ordered Locus Names:YFR014C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VI

Organism-specific databases

CYGDiYFR014c.
EuPathDBiFungiDB:YFR014C.
SGDiS000001910. CMK1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Calcium/calmodulin-dependent protein kinase IPRO_0000086090Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei343 – 3431PhosphotyrosineBy similarity
Modified residuei357 – 3571PhosphoserineBy similarity
Modified residuei417 – 4171Phosphoserine2 Publications
Modified residuei418 – 4181Phosphoserine1 Publication
Modified residuei420 – 4201Phosphoserine2 Publications
Modified residuei429 – 4291Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27466.
PaxDbiP27466.
PeptideAtlasiP27466.

Interactioni

Subunit structurei

Multimeric.

Protein-protein interaction databases

BioGridi31166. 235 interactions.
DIPiDIP-2305N.
IntActiP27466. 33 interactions.
MINTiMINT-687892.
STRINGi4932.YFR014C.

Structurei

3D structure databases

ProteinModelPortaliP27466.
SMRiP27466. Positions 14-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 299263Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni312 – 32312Calmodulin-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
InParanoidiP27466.
KOiK08794.
OMAiFESKDKY.
OrthoDBiEOG793BK1.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDKVSEKES SPKQTEEDSE GKMAHVQPAS YVNKKKYVFG KTLGAGTFGV
60 70 80 90 100
VRQAKNTETG EDVAVKILIK KALKGNKVQL EALYDELDIL QRLHHPNIVA
110 120 130 140 150
FKDWFESKDK FYIITQLAKG GELFDRILKK GKFTEEDAVR ILVEILSAVK
160 170 180 190 200
YMHSQNIVHR DLKPENLLYI DKSDESPLVV ADFGIAKRLK SDEELLYKPA
210 220 230 240 250
GSLGYVAPEV LTQDGHGKPC DIWSIGVITY TLLCGYSAFR AERVQDFLDE
260 270 280 290 300
CTTGEYPVKF HRPYWDSVSN KAKQFILKAL NLDPSKRPTA AELLEDPWII
310 320 330 340 350
CTELKTHNLL PGLKEGLDAR QKFRNSVERV RLNMKIQKLR DLYLEQTESD
360 370 380 390 400
SDFDEGSQAN GSVPPLKATD TSQLSKKLSE EEQSKLKSEL TSKAFAQLVN
410 420 430 440
TVLAEKEKFL NINRVCSSDS DLPGSDIKSL DEAKEKPEGK DTKTEE
Length:446
Mass (Da):50,296
Last modified:November 1, 1995 - v2
Checksum:i586C31CED6311555
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 542QA → VR in CAA40928 (PubMed:2026147).Curated
Sequence conflicti68 – 681L → V in CAA40928 (PubMed:2026147).Curated
Sequence conflicti188 – 1881R → T in CAA40928 (PubMed:2026147).Curated
Sequence conflicti199 – 2024PAGS → AGTA in CAA40928 (PubMed:2026147).Curated
Sequence conflicti237 – 2437SAFRAER → ATIDRK in CAA40928 (PubMed:2026147).Curated
Sequence conflicti271 – 2711K → Q in CAA40928 (PubMed:2026147).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57782 Genomic DNA. Translation: CAA40928.1.
D90375 Genomic DNA. Translation: BAA14383.1.
D50617 Genomic DNA. Translation: BAA09253.1.
BK006940 Genomic DNA. Translation: DAA12455.1.
PIRiA40896.
RefSeqiNP_116669.1. NM_001179979.2.

Genome annotation databases

EnsemblFungiiYFR014C; YFR014C; YFR014C.
GeneIDi850568.
KEGGisce:YFR014C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57782 Genomic DNA. Translation: CAA40928.1.
D90375 Genomic DNA. Translation: BAA14383.1.
D50617 Genomic DNA. Translation: BAA09253.1.
BK006940 Genomic DNA. Translation: DAA12455.1.
PIRiA40896.
RefSeqiNP_116669.1. NM_001179979.2.

3D structure databases

ProteinModelPortaliP27466.
SMRiP27466. Positions 14-323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31166. 235 interactions.
DIPiDIP-2305N.
IntActiP27466. 33 interactions.
MINTiMINT-687892.
STRINGi4932.YFR014C.

Proteomic databases

MaxQBiP27466.
PaxDbiP27466.
PeptideAtlasiP27466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYFR014C; YFR014C; YFR014C.
GeneIDi850568.
KEGGisce:YFR014C.

Organism-specific databases

CYGDiYFR014c.
EuPathDBiFungiDB:YFR014C.
SGDiS000001910. CMK1.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
InParanoidiP27466.
KOiK08794.
OMAiFESKDKY.
OrthoDBiEOG793BK1.

Enzyme and pathway databases

BioCyciYEAST:G3O-30467-MONOMER.
BRENDAi2.7.11.17. 984.
ReactomeiREACT_336144. CREB phosphorylation through the activation of CaMKII.
REACT_353242. HSF1-dependent transactivation.

Miscellaneous databases

NextBioi966378.
PROiP27466.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple Ca2+/calmodulin-dependent protein kinase genes in a unicellular eukaryote."
    Pausch M.H., Kaim D., Kunisawa R., Admon A., Thorner J.
    EMBO J. 10:1511-1522(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Two yeast genes encoding calmodulin-dependent protein kinases. Isolation, sequencing and bacterial expressions of CMK1 and CMK2."
    Ohya Y., Kawasaki H., Suzuki K., Londesborough J., Anraku Y.
    J. Biol. Chem. 266:12784-12794(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417; SER-420 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417; SER-418; SER-420 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKCC1_YEAST
AccessioniPrimary (citable) accession number: P27466
Secondary accession number(s): D6VTP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1995
Last modified: June 24, 2015
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.