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P27466 (KCC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium/calmodulin-dependent protein kinase I

EC=2.7.11.17
Gene names
Name:CMK1
Ordered Locus Names:YFR014C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Important in cellular regulation.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Multimeric.

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446Calcium/calmodulin-dependent protein kinase I
PRO_0000086090

Regions

Domain37 – 299263Protein kinase
Nucleotide binding43 – 519ATP By similarity
Region312 – 32312Calmodulin-binding By similarity

Sites

Active site1611Proton acceptor By similarity
Binding site661ATP By similarity

Amino acid modifications

Modified residue4171Phosphoserine Ref.6 Ref.8
Modified residue4181Phosphoserine Ref.8
Modified residue4201Phosphoserine Ref.6 Ref.8
Modified residue4291Phosphoserine Ref.6 Ref.7 Ref.8

Experimental info

Sequence conflict53 – 542QA → VR in CAA40928. Ref.1
Sequence conflict681L → V in CAA40928. Ref.1
Sequence conflict1881R → T in CAA40928. Ref.1
Sequence conflict199 – 2024PAGS → AGTA in CAA40928. Ref.1
Sequence conflict237 – 2437SAFRAER → ATIDRK in CAA40928. Ref.1
Sequence conflict2711K → Q in CAA40928. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P27466 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 586C31CED6311555

FASTA44650,296
        10         20         30         40         50         60 
MDDKVSEKES SPKQTEEDSE GKMAHVQPAS YVNKKKYVFG KTLGAGTFGV VRQAKNTETG 

        70         80         90        100        110        120 
EDVAVKILIK KALKGNKVQL EALYDELDIL QRLHHPNIVA FKDWFESKDK FYIITQLAKG 

       130        140        150        160        170        180 
GELFDRILKK GKFTEEDAVR ILVEILSAVK YMHSQNIVHR DLKPENLLYI DKSDESPLVV 

       190        200        210        220        230        240 
ADFGIAKRLK SDEELLYKPA GSLGYVAPEV LTQDGHGKPC DIWSIGVITY TLLCGYSAFR 

       250        260        270        280        290        300 
AERVQDFLDE CTTGEYPVKF HRPYWDSVSN KAKQFILKAL NLDPSKRPTA AELLEDPWII 

       310        320        330        340        350        360 
CTELKTHNLL PGLKEGLDAR QKFRNSVERV RLNMKIQKLR DLYLEQTESD SDFDEGSQAN 

       370        380        390        400        410        420 
GSVPPLKATD TSQLSKKLSE EEQSKLKSEL TSKAFAQLVN TVLAEKEKFL NINRVCSSDS 

       430        440 
DLPGSDIKSL DEAKEKPEGK DTKTEE 

« Hide

References

« Hide 'large scale' references
[1]"Multiple Ca2+/calmodulin-dependent protein kinase genes in a unicellular eukaryote."
Pausch M.H., Kaim D., Kunisawa R., Admon A., Thorner J.
EMBO J. 10:1511-1522(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Two yeast genes encoding calmodulin-dependent protein kinases. Isolation, sequencing and bacterial expressions of CMK1 and CMK2."
Ohya Y., Kawasaki H., Suzuki K., Londesborough J., Anraku Y.
J. Biol. Chem. 266:12784-12794(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417; SER-420 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417; SER-418; SER-420 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X57782 Genomic DNA. Translation: CAA40928.1.
D90375 Genomic DNA. Translation: BAA14383.1.
D50617 Genomic DNA. Translation: BAA09253.1.
BK006940 Genomic DNA. Translation: DAA12455.1.
PIRA40896.
RefSeqNP_116669.1. NM_001179979.2.

3D structure databases

ProteinModelPortalP27466.
SMRP27466. Positions 14-323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31166. 234 interactions.
DIPDIP-2305N.
IntActP27466. 33 interactions.
MINTMINT-687892.
STRING4932.YFR014C.

Proteomic databases

MaxQBP27466.
PaxDbP27466.
PeptideAtlasP27466.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFR014C; YFR014C; YFR014C.
GeneID850568.
KEGGsce:YFR014C.

Organism-specific databases

CYGDYFR014c.
SGDS000001910. CMK1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00750000117487.
HOGENOMHOG000233016.
KOK08794.
OMAFESKDKY.
OrthoDBEOG793BK1.

Enzyme and pathway databases

BioCycYEAST:G3O-30467-MONOMER.
BRENDA2.7.11.17. 984.

Gene expression databases

GenevestigatorP27466.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24347. PTHR24347. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966378.
PROP27466.

Entry information

Entry nameKCC1_YEAST
AccessionPrimary (citable) accession number: P27466
Secondary accession number(s): D6VTP5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families