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Protein

Calcium/calmodulin-dependent protein kinase I

Gene

CMK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Important in cellular regulation.

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei66ATPPROSITE-ProRule annotation1
Active sitei161Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi43 – 51ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin binding Source: GO_Central
  • calmodulin-dependent protein kinase activity Source: SGD

GO - Biological processi

  • cellular response to oxidative stress Source: GO_Central
  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • peptidyl-threonine phosphorylation Source: GO_Central
  • protein phosphorylation Source: SGD
  • regulation of cell cycle Source: GO_Central
  • signal transduction Source: SGD

Keywordsi

Molecular functionCalmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30467-MONOMER
BRENDAi2.7.11.17 984
ReactomeiR-SCE-111932 CaMK IV-mediated phosphorylation of CREB
R-SCE-3371571 HSF1-dependent transactivation
R-SCE-4086398 Ca2+ pathway
R-SCE-442729 CREB phosphorylation through the activation of CaMKII
R-SCE-442745 Activation of CaMK IV
R-SCE-5578775 Ion homeostasis
R-SCE-936837 Ion transport by P-type ATPases

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase I (EC:2.7.11.17)
Gene namesi
Name:CMK1
Ordered Locus Names:YFR014C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR014C
SGDiS000001910 CMK1

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860901 – 446Calcium/calmodulin-dependent protein kinase IAdd BLAST446

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei343PhosphotyrosineBy similarity1
Modified residuei357PhosphoserineBy similarity1
Modified residuei417PhosphoserineCombined sources1
Modified residuei418PhosphoserineCombined sources1
Modified residuei420PhosphoserineCombined sources1
Modified residuei429PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27466
PaxDbiP27466
PRIDEiP27466

PTM databases

iPTMnetiP27466

Interactioni

Subunit structurei

Multimeric.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31166, 291 interactors
DIPiDIP-2305N
IntActiP27466, 34 interactors
MINTiP27466
STRINGi4932.YFR014C

Structurei

3D structure databases

ProteinModelPortaliP27466
SMRiP27466
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 299Protein kinasePROSITE-ProRule annotationAdd BLAST263

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni312 – 323Calmodulin-bindingBy similarityAdd BLAST12

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00760000118944
HOGENOMiHOG000233016
InParanoidiP27466
KOiK08794
OMAiPHIVKFV
OrthoDBiEOG092C0QJU

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P27466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDKVSEKES SPKQTEEDSE GKMAHVQPAS YVNKKKYVFG KTLGAGTFGV
60 70 80 90 100
VRQAKNTETG EDVAVKILIK KALKGNKVQL EALYDELDIL QRLHHPNIVA
110 120 130 140 150
FKDWFESKDK FYIITQLAKG GELFDRILKK GKFTEEDAVR ILVEILSAVK
160 170 180 190 200
YMHSQNIVHR DLKPENLLYI DKSDESPLVV ADFGIAKRLK SDEELLYKPA
210 220 230 240 250
GSLGYVAPEV LTQDGHGKPC DIWSIGVITY TLLCGYSAFR AERVQDFLDE
260 270 280 290 300
CTTGEYPVKF HRPYWDSVSN KAKQFILKAL NLDPSKRPTA AELLEDPWII
310 320 330 340 350
CTELKTHNLL PGLKEGLDAR QKFRNSVERV RLNMKIQKLR DLYLEQTESD
360 370 380 390 400
SDFDEGSQAN GSVPPLKATD TSQLSKKLSE EEQSKLKSEL TSKAFAQLVN
410 420 430 440
TVLAEKEKFL NINRVCSSDS DLPGSDIKSL DEAKEKPEGK DTKTEE
Length:446
Mass (Da):50,296
Last modified:November 1, 1995 - v2
Checksum:i586C31CED6311555
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53 – 54QA → VR in CAA40928 (PubMed:2026147).Curated2
Sequence conflicti68L → V in CAA40928 (PubMed:2026147).Curated1
Sequence conflicti188R → T in CAA40928 (PubMed:2026147).Curated1
Sequence conflicti199 – 202PAGS → AGTA in CAA40928 (PubMed:2026147).Curated4
Sequence conflicti237 – 243SAFRAER → ATIDRK in CAA40928 (PubMed:2026147).Curated7
Sequence conflicti271K → Q in CAA40928 (PubMed:2026147).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57782 Genomic DNA Translation: CAA40928.1
D90375 Genomic DNA Translation: BAA14383.1
D50617 Genomic DNA Translation: BAA09253.1
BK006940 Genomic DNA Translation: DAA12455.1
PIRiA40896
RefSeqiNP_116669.1, NM_001179979.2

Genome annotation databases

EnsemblFungiiBAA09253; BAA09253; BAA09253
YFR014C; YFR014C; YFR014C
GeneIDi850568
KEGGisce:YFR014C

Similar proteinsi

Entry informationi

Entry nameiKCC1_YEAST
AccessioniPrimary (citable) accession number: P27466
Secondary accession number(s): D6VTP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

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