P27462 (RDRP_BBMV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase 2a
Short name=protein 2a
EC=2.7.7.48

Gene names

ORF Names:

ORF2a

Organism

Broad bean mottle virus [Complete proteome]

Taxonomic identifier

12301 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Bromoviridae › Bromovirus

Virus host

Vicia faba (Broad bean) (Faba vulgaris) [TaxID: 3906]

Protein attributes

Sequence length	810 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which replicates the viral genome composed of 3 RNA segments, RNA1, RNA2 and RNA3 Potential.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Interacts with replication protein 1a By similarity.
Sequence similarities	Belongs to the ssRNA positive-strand viruses RNA-directed RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 810	810	RNA-directed RNA polymerase 2a		PRO_0000083268

Sequences

	Sequence		Length	Mass (Da)
	P27462 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: BDB1A5EB27D532AE	FASTA	810	92,993
	10 20 30 40 50 60 MSKFVAADEY IVPSFQWLLG PTSSQVESFD SSVAGWIKRY EDESKRPEST VDGSCESFVL 70 80 90 100 110 120 AVKPVMIGGQ CEPAYDQAKW AETCLNVTNL ASGLTGVRLI PLPEMARMIY LDEEDSFVDE 130 140 150 160 170 180 SEVDDWYPED TSDGFEYLSA DGSDYHQNSE PEEEVLDKSN GTLESEEARH ETDVKSICSF 190 200 210 220 230 240 RDIPVETTMG HRYLALSEEF ASIEVDYQVS SIVTPLNKGS IYMTLDVHEE THPKSGIQDK 250 260 270 280 290 300 ASIERLEAAG HNALKTHAYF DDSYYEGFEE SADFSSDFQR LKIKQSHVDW YKDPDKFFEP 310 320 330 340 350 360 VLNFGASSRR VGSQKTVLTA LKKRNADVPE LSDVVDATEV PICLRQSYLN IYGADCLFES 370 380 390 400 410 420 FNIMAKGLDY HKRWKSHKEL QGVTLLCETN LQRYQHMIKS DVKPTVTDTL HVERDVPATI 430 440 450 460 470 480 TFHGKGVTSC FSPFFTACFE KFSLALRESI YVPIGKISSL ELKSKALNNK FFLEADLSKF 490 500 510 520 530 540 DKSQGELHLE FQRLILINLG FPVPLTNWWC DFHRSILSRW TLRPGVSIPL LSKTNRGCFH 550 560 570 580 590 600 LLWEHFSDNA MMAYCFDMST AELAMFSGDD SLVICGSKPE FDPGVFQSLF NMEVKVMDPS 610 620 630 640 650 660 LPYICSKFLL ESEFGDVFSV PDPMREIQRL QKRKIPKDVQ VLRADFDSFC DRMKFLDRLS 670 680 690 700 710 720 ELSLSVLCRL TALKYCKPGI EGDVRAWLSG FAYYRENFLR YSECYVTDGI HCYRRVDPMS 730 740 750 760 770 780 RFKPIDKFQR SDKEWFHDWR NNEFPKKPID KMARMFGAYK GPVNNDRVER KAKYKVNAAM 790 800 810 HDPFALAYER RYVQELELKN DKGNCSVLRE

References

[1]	"The nucleotide sequence and genome organization of the RNA2 and RNA3 segments in broad bean mottle virus." Romero J., Dzianott A.M., Bujarski J.J. Virology 187:671-681(1992) [PubMed: 1546462] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64713 Genomic RNA. Translation: AAA42741.1.
PIR	P2WMBB. A42453.
RefSeq	NP_658999.1. NC_004007.1.
3D structure databases
ProteinModelPortal	P27462.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	962139.
Family and domain databases
InterPro	IPR007610. BBMV_Gp1_N. IPR001788. Tymovirus_RNA-dep_RNA_pol. [Graphical view]
Pfam	PF04522. DUF585. 1 hit. PF00978. RdRP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_BBMV

Accession

Primary (citable) accession number: P27462

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	October 19, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program