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Protein

Gag polyprotein

Gene

gag

Organism
Cas-Br-E murine leukemia virus
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Gag polyprotein: Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release.By similarity
Matrix protein p15: Targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the pre-integration complex.By similarity
RNA-binding phosphoprotein p12: Constituent of the pre-integration complex (PIC) which tethers the latter to mitotic chromosomes.By similarity
Capsid protein p30: Forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p10-Gag: Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri500 – 517CCHC-typePROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Viral nucleoprotein
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Gene namesi
Name:gag
OrganismiCas-Br-E murine leukemia virus
Taxonomic identifieri11792 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]

Subcellular locationi

Gag polyprotein :
  • Virion By similarity
  • Host cell membrane By similarity; Lipid-anchor By similarity
  • host multivesicular body By similarity
Matrix protein p15 :
  • Virion By similarity
Capsid protein p30 :
  • Virion By similarity
Nucleocapsid protein p10-Gag :
  • Virion By similarity
RNA-binding phosphoprotein p12 :
  • Host cytoplasm By similarity
  • Note: Localizes to the host cytoplasm early in infection and binds to the mitotic chromosomes later on.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host cytoplasm, Host endosome, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostSequence analysis
ChainiPRO_00003908082 – 536Gag polyproteinBy similarityAdd BLAST535
ChainiPRO_00000408842 – 129Matrix protein p15Add BLAST128
ChainiPRO_0000040885130 – 213RNA-binding phosphoprotein p12Add BLAST84
ChainiPRO_0000040886214 – 476Capsid protein p30Add BLAST263
ChainiPRO_0000040887477 – 536Nucleocapsid protein p10-GagSequence analysisAdd BLAST60

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostSequence analysis1
Modified residuei190Phosphoserine; by hostBy similarity1

Post-translational modificationi

Gag polyprotein: Ubiquitinated by ITCH. Gag can recruit the ubiquitin ligase Itch in an L domain-independent manner to facilitate virus release via a mechanism that involves Gag ubiquitination.By similarity
Gag polyprotein: Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation.By similarity
Capsid protein p30: Sumoylated; required for virus replication.By similarity
RNA-binding phosphoprotein p12 is phosphorylated on serine residues.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei129 – 130Cleavage; by viral proteaseBy similarity2
Sitei213 – 214Cleavage; by viral proteaseBy similarity2
Sitei476 – 477Cleavage; by viral proteaseBy similarity2

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Capsid protein p30: Homohexamer; further associates as homomultimer (By similarity). Capsid protein p30: The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers. Capsid protein p30: Interacts with mouse UBE2I and mouse PIAS4. Gag polyprotein: Interacts (via PPXY motif) with host NEDD4. Gag polyprotein: Interacts (via PSAP motif) with host TSG101. Gag polyprotein: Interacts (via LYPX(n)L motif) with host PDCD6IP (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP27460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni343 – 391Interaction with host PIAS4By similarityAdd BLAST49
Regioni428 – 433Interaction with host UBE2IBy similarity6

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili436 – 476Sequence analysisAdd BLAST41

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi109 – 112PTAP/PSAP motifBy similarity4
Motifi128 – 132LYPX(n)L motifBy similarity5
Motifi160 – 163PPXY motifBy similarity4

Domaini

Gag polyprotein: Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. PPXY motif is essential for virus egress. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L motif which interacts with PDCD6IP. Both PSAP and LYPX(n)L domains might play little to no role in budding and possibly drive residual virus release.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri500 – 517CCHC-typePROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Coiled coil, Zinc-finger

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiView protein in InterPro
IPR000840. G_retro_matrix.
IPR036946. G_retro_matrix_sf.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR001878. Znf_CCHC.
IPR036875. Znf_CCHC_sf.
PfamiView protein in Pfam
PF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
PF00098. zf-CCHC. 1 hit.
SMARTiView protein in SMART
SM00343. ZnF_C2HC. 1 hit.
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiView protein in PROSITE
PS50158. ZF_CCHC. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Gag polyprotein (identifier: P27460-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQTVTTPLS LTLDHWKDVE RTAHNQSVDV KKRRWVTFCS VEWPTFNVGW
60 70 80 90 100
PQDGTFNRDI ITQVKIKVFS PGPHGHPDQV PYIVTWEALA FDPPPWVKPF
110 120 130 140 150
VHPKPPLPPS APSLLPEPPL STSPRSSLYP ALTPSLGAKP KPQVLPDSGG
160 170 180 190 200
PLIDLLTEDP PPYRDPGPPP SDRDRDDGEA APAGEAPDPS PMASRLRGRR
210 220 230 240 250
ELPVADSTTS QAFPLRSGGN GQLQYWPFSS SDLYNWKNNN PSFSEDPGKL
260 270 280 290 300
TALIESVLLT HQPTWDDCQQ LLGTLLTGEE KQRVLLEARK AVRGEDGRPT
310 320 330 340 350
QLPNEINDAF PLERPDWDYN TQRGRNHLVL YRQLLLAGLQ NAGRSPTNLA
360 370 380 390 400
KVKGITQGPN ESPSAFLERL KEAYRRYTPY DPEDPGQETN VSMSFIWQSA
410 420 430 440 450
PDIGRKLERL EDLKSKTLGD LVREAEKIFN KRETPEEREE RIKRETEEKE
460 470 480 490 500
ERRRAEDEQK EKERDRRRHR EMSKLLATVV SGQKQDRQGG ERRRPQLDKD
510 520 530
QCAYCKEKGH WAKDCPKKPR GPRGPRPQTS LLALDD
Length:536
Mass (Da):60,740
Last modified:January 23, 2007 - v3
Checksum:i3E0F863A393960AE
GO
Isoform Glyco-Gag protein (identifier: P0DOH4-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P0DOH4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:624
Mass (Da):70,325
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57540 Genomic DNA. Translation: CAA40759.1.

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiGAG_MLVCB
AccessioniPrimary (citable) accession number: P27460
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: January 31, 2018
This is version 104 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program