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P27456 (GSHRP_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione reductase, chloroplastic/mitochondrial

Short name=GR
Short name=GRase
EC=1.8.1.7
Alternative name(s):
GOR1
Gene names
Name:GR
Synonyms:GOR1
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length552 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Maintains high levels of reduced glutathione in the chloroplast.

Catalytic activity

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactor

Binds 1 FAD per subunit By similarity.

Subcellular location

Plastidchloroplast. Mitochondrion. Note: The majority of the protein is found in chloroplast, with only 3% in mitochondria. Ref.3

Miscellaneous

The active site is a redox-active disulfide bond.

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Sequence caution

The sequence CAA42921.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6060Chloroplast and mitochondrion Potential
Chain61 – 552492Glutathione reductase, chloroplastic/mitochondrial
PRO_0000030281

Regions

Nucleotide binding112 – 12110FAD By similarity

Sites

Active site5151Proton acceptor By similarity
Binding site2771NADP By similarity
Binding site2831NADP By similarity

Amino acid modifications

Disulfide bond121 ↔ 126Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
P27456 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 690D1058AE4168BC

FASTA55259,108
        10         20         30         40         50         60 
MNQAMATPLS LSCCSPTLTR STLFFTKTFP FSRSFSTPLP LSTKTLISLS PPHRTFAVRA 

        70         80         90        100        110        120 
ESQNGADPAR QYDFDLFTIG AGSGGVRASR FASNFGASSA VCELPFSTIS SDTTGGVGGT 

       130        140        150        160        170        180 
CVIRGCVPKK LLVYASKFSH EFEESNGFGW RYDSEPKHDW SSLIANKNAE LQRLTGIYKN 

       190        200        210        220        230        240 
TLKNAGVKLI EGRGKIVDAH TVDVDGKLYS AKHILVSVGG RPFIPDIPGK EYAIDSDAAL 

       250        260        270        280        290        300 
DLPSKPQKIA IVGGGYIALE FAGIFNGLKS EVHVFIRQKK VLRGFDEEIR DFVAENMALR 

       310        320        330        340        350        360 
GIEFHTEESP VAITKAADGS LSLKTNKGTE EGFSHIMFAT GRSPNTKDLG LESVGVKVAK 

       370        380        390        400        410        420 
DGSIEVDEYS QTSVPSIWAI GDATNRVNLT PVALMEGVAL AKTLFQNEPT KPDYRAIPSA 

       430        440        450        460        470        480 
VFSQPPIGGV GLTEEQAAEQ YGDIDVFTAN FRPMKATLSG LPDRVFMKLI VSAETNVVLG 

       490        500        510        520        530        540 
LHMCGEDAAE IAQGFAVGIK AGLTKADFDA TVGIHPTAAE EFVTMRTPTR KVRKNQASQG 

       550 
KSDSKAKAVA GS 

« Hide

References

[1]"Molecular characterization of glutathione reductase cDNAs from pea (Pisum sativum L.)."
Creissen G., Edwards E.A., Enard C., Wellburn A., Mullineaux P.M.
Plant J. 2:129-131(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Leaf.
[2]"Characterisation of a glutathione reductase gene and its genetic locus from pea (Pisum sativum L.)."
Mullineaux P.M., Enard C., Hellens R., Creissen G.
Planta 200:186-194(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Birte.
[3]"Simultaneous targeting of pea glutathione reductase and of a bacterial fusion protein to chloroplasts and mitochondria in transgenic tobacco."
Creissen G.P., Reynolds H., Xue Y., Mullineaux P.M.
Plant J. 8:167-175(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60373 mRNA. Translation: CAA42921.1. Different initiation.
X90996 Genomic DNA. Translation: CAA62482.1.
PIRS18973.

3D structure databases

ProteinModelPortalP27456.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.390.30. 1 hit.
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. SSF55424. 1 hit.
TIGRFAMsTIGR01424. gluta_reduc_2. 1 hit.
PROSITEPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHRP_PEA
AccessionPrimary (citable) accession number: P27456
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: February 19, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families