Reviewed, UniProtKB/Swiss-Prot P27456 (GSHRP_PEA)

Names and origin

Protein names

Recommended name:
    Glutathione reductase, chloroplastic/mitochondrial
      Short name=GRase
      Short name=GR
    EC=1.8.1.7
Alternative name(s):
    GOR1

Gene names

Name:	GR
Synonyms:	GOR1

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the chloroplast.
Catalytic activity	2 glutathione + NADP+ = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Plastid › chloroplast. Mitochondrion. Note: The majority of the protein is found in chloroplast, with only 3% in mitochondria. Ref.3
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Chloroplast Mitochondrion Plastid
Domain	Redox-active center Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 60	60	Chloroplast and mitochondrion Potential
	Chain	61 – 552	492	Glutathione reductase, chloroplastic/mitochondrial		PRO_0000030281
Regions
	Nucleotide binding	112 – 121	10	FAD By similarity
Sites
	Active site	515	1	Proton acceptor By similarity
	Binding site	277	1	NADP By similarity
	Binding site	283	1	NADP By similarity
Amino acid modifications
	Disulfide bond	121 ↔ 126		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27456-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 690D1058AE4168BC	FASTA	552	59,108
	10 20 30 40 50 60 MNQAMATPLS LSCCSPTLTR STLFFTKTFP FSRSFSTPLP LSTKTLISLS PPHRTFAVRA 70 80 90 100 110 120 ESQNGADPAR QYDFDLFTIG AGSGGVRASR FASNFGASSA VCELPFSTIS SDTTGGVGGT 130 140 150 160 170 180 CVIRGCVPKK LLVYASKFSH EFEESNGFGW RYDSEPKHDW SSLIANKNAE LQRLTGIYKN 190 200 210 220 230 240 TLKNAGVKLI EGRGKIVDAH TVDVDGKLYS AKHILVSVGG RPFIPDIPGK EYAIDSDAAL 250 260 270 280 290 300 DLPSKPQKIA IVGGGYIALE FAGIFNGLKS EVHVFIRQKK VLRGFDEEIR DFVAENMALR 310 320 330 340 350 360 GIEFHTEESP VAITKAADGS LSLKTNKGTE EGFSHIMFAT GRSPNTKDLG LESVGVKVAK 370 380 390 400 410 420 DGSIEVDEYS QTSVPSIWAI GDATNRVNLT PVALMEGVAL AKTLFQNEPT KPDYRAIPSA 430 440 450 460 470 480 VFSQPPIGGV GLTEEQAAEQ YGDIDVFTAN FRPMKATLSG LPDRVFMKLI VSAETNVVLG 490 500 510 520 530 540 LHMCGEDAAE IAQGFAVGIK AGLTKADFDA TVGIHPTAAE EFVTMRTPTR KVRKNQASQG 550 KSDSKAKAVA GS

References

[1]	"Molecular characterization of glutathione reductase cDNAs from pea (Pisum sativum L.)." Creissen G., Edwards E.A., Enard C., Wellburn A., Mullineaux P.M. Plant J. 2:129-131(1992) [PubMed: 1303792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[2]	"Characterisation of a glutathione reductase gene and its genetic locus from pea (Pisum sativum L.)." Mullineaux P.M., Enard C., Hellens R., Creissen G. Planta 200:186-194(1996) [PubMed: 8904805] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Birte.
[3]	"Simultaneous targeting of pea glutathione reductase and of a bacterial fusion protein to chloroplasts and mitochondria in transgenic tobacco." Creissen G.P., Reynolds H., Xue Y., Mullineaux P.M. Plant J. 8:167-175(1995) [PubMed: 7670502] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60373 mRNA. Translation: CAA42921.1. Different initiation. X90996 Genomic DNA. Translation: CAA62482.1.
PIR	S18973.
3D structure databases
SMR	P27456. Positions 73-532.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.8.1.7. 287.
Family and domain databases
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006324. Glut_reduct_pln. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
TIGRFAMs	TIGR01424. gluta_reduc_2. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHRP_PEA

Accession

Primary (citable) accession number: P27456

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	February 9, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)