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Protein

Probable serine/threonine-protein kinase CST

Gene

CST

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a spatial inhibitor of signaling that modulates abscission zone cell adhesion and expansion. Acts both directly and indirectly by physically interacting with RLK5/HAE and SOBIR1/EVR at the cell surface.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei124ATPPROSITE-ProRule annotation1
Active sitei218Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi92 – 100ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell surface receptor signaling pathway Source: GO_Central
  • negative regulation of floral organ abscission Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable serine/threonine-protein kinase CSTCurated (EC:2.7.11.1Curated)
Alternative name(s):
Protein CAST AWAY1 Publication
Gene namesi
Name:CST1 Publication
Synonyms:CX321 Publication, KIN41 Publication
Ordered Locus Names:At4g35600
ORF Names:F8D20.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G35600.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Drastic reduction of plasma membrane localization and strong increase of nuclear localization. Partial redistribution from the plasma membrane to the cytoplasm. 2 Publications1
Mutagenesisi4C → S: Partial redistribution from the plasma membrane to the cytoplasm. 1 Publication1
Mutagenesisi124K → A: Abolishes kinase activity. 1 Publication1
Mutagenesisi157G → R in cst-1; abolishes kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000243212 – 419Probable serine/threonine-protein kinase CSTAdd BLAST418

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine2 Publications1
Lipidationi4S-palmitoyl cysteine1 Publication1
Modified residuei117PhosphoserineCombined sources1
Modified residuei169PhosphotyrosineBy similarity1
Modified residuei222PhosphoserineBy similarity1
Modified residuei253PhosphothreonineBy similarity1
Modified residuei258PhosphothreonineBy similarity1
Modified residuei266PhosphotyrosineBy similarity1

Post-translational modificationi

Autophosphorylated on serine, threonine and tyrosine residues.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP27450.

PTM databases

iPTMnetiP27450.

Expressioni

Gene expression databases

ExpressionAtlasiP27450. baseline and differential.
GenevisibleiP27450. AT.

Interactioni

Subunit structurei

Interacts with SOBIR1/EVR and RLK5/HAE.1 Publication

Protein-protein interaction databases

BioGridi14994. 3 interactors.
IntActiP27450. 8 interactors.
STRINGi3702.AT4G35600.2.

Structurei

3D structure databases

ProteinModelPortaliP27450.
SMRiP27450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 368Protein kinasePROSITE-ProRule annotationAdd BLAST283

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 14Poly-Ser6

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiP27450.
KOiK00924.
PhylomeDBiP27450.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF07714. Pkinase_Tyr. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P27450-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGACISFFSS SSPSKTGLHS HATTNNHSNG TEFSSTTGAT TNSSVGQQSQ
60 70 80 90 100
FSDISTGIIS DSGKLLESPN LKVYNFLDLK TATKNFKPDS MLGQGGFGKV
110 120 130 140 150
YRGWVDATTL APSRVGSGMI VAIKRLNSES VQGFAEWRSE VNFLGMLSHR
160 170 180 190 200
NLVKLLGYCR EDKELLLVYE FMPKGSLESH LFRRNDPFPW DLRIKIVIGA
210 220 230 240 250
ARGLAFLHSL QREVIYRDFK ASNILLDSNY DAKLSDFGLA KLGPADEKSH
260 270 280 290 300
VTTRIMGTYG YAAPEYMATG HLYVKSDVFA FGVVLLEIMT GLTAHNTKRP
310 320 330 340 350
RGQESLVDWL RPELSNKHRV KQIMDKGIKG QYTTKVATEM ARITLSCIEP
360 370 380 390 400
DPKNRPHMKE VVEVLEHIQG LNVVPNRSST KQAVANSSRS SPHHYRYKAG
410
ALGAERKRAT PGRFGSVEK
Length:419
Mass (Da):46,340
Last modified:June 21, 2004 - v2
Checksum:i7C85AB6C38925145
GO

Sequence cautioni

The sequence AAA32850 differs from that shown. Sequencing errors.Curated
The sequence AAA32850 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence CAA20030 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80276 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63234 mRNA. Translation: AAA32850.1. Sequence problems.
AL031135 Genomic DNA. Translation: CAA20030.1. Sequence problems.
AL161587 Genomic DNA. Translation: CAB80276.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86538.1.
AY065403 mRNA. Translation: AAL38844.1.
AY096501 mRNA. Translation: AAM20151.1.
PIRiA39357.
C85420.
T04665.
RefSeqiNP_195285.3. NM_119725.5. [P27450-1]
UniGeneiAt.57035.

Genome annotation databases

EnsemblPlantsiAT4G35600.1; AT4G35600.1; AT4G35600. [P27450-1]
GeneIDi829712.
GrameneiAT4G35600.1; AT4G35600.1; AT4G35600.
KEGGiath:AT4G35600.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCST_ARATH
AccessioniPrimary (citable) accession number: P27450
Secondary accession number(s): O81792, Q8VZ07, Q9M068
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: June 21, 2004
Last modified: August 30, 2017
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was originally (PubMed:1851993) reported to be a connexin and to contain transmembrane domains. PubMed:8400879 authors have assigned that this is not a connexin, but rather a protein kinase.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families