Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable serine/threonine-protein kinase Cx32, chloroplastic

Gene

At4g35600

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei124 – 1241ATPPROSITE-ProRule annotation
Active sitei218 – 2181Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi92 – 1009ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G35600-MONOMER.
ARA:GQT-2693-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable serine/threonine-protein kinase Cx32, chloroplastic (EC:2.7.11.1)
Gene namesi
Ordered Locus Names:At4g35600
ORF Names:F8D20.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G35600.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4242ChloroplastSequence analysisAdd
BLAST
Chaini43 – 419377Probable serine/threonine-protein kinase Cx32, chloroplasticPRO_0000024321Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei117 – 1171PhosphoserineCombined sources
Modified residuei253 – 2531PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP27450.
PRIDEiP27450.

PTM databases

iPTMnetiP27450.

Expressioni

Gene expression databases

ExpressionAtlasiP27450. baseline and differential.
GenevisibleiP27450. AT.

Interactioni

Protein-protein interaction databases

BioGridi14994. 3 interactions.
IntActiP27450. 8 interactions.
STRINGi3702.AT4G35600.2.

Structurei

3D structure databases

ProteinModelPortaliP27450.
SMRiP27450. Positions 21-391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 368283Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi9 – 146Poly-Ser

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiP27450.
KOiK00924.
PhylomeDBiP27450.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P27450-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGACISFFSS SSPSKTGLHS HATTNNHSNG TEFSSTTGAT TNSSVGQQSQ
60 70 80 90 100
FSDISTGIIS DSGKLLESPN LKVYNFLDLK TATKNFKPDS MLGQGGFGKV
110 120 130 140 150
YRGWVDATTL APSRVGSGMI VAIKRLNSES VQGFAEWRSE VNFLGMLSHR
160 170 180 190 200
NLVKLLGYCR EDKELLLVYE FMPKGSLESH LFRRNDPFPW DLRIKIVIGA
210 220 230 240 250
ARGLAFLHSL QREVIYRDFK ASNILLDSNY DAKLSDFGLA KLGPADEKSH
260 270 280 290 300
VTTRIMGTYG YAAPEYMATG HLYVKSDVFA FGVVLLEIMT GLTAHNTKRP
310 320 330 340 350
RGQESLVDWL RPELSNKHRV KQIMDKGIKG QYTTKVATEM ARITLSCIEP
360 370 380 390 400
DPKNRPHMKE VVEVLEHIQG LNVVPNRSST KQAVANSSRS SPHHYRYKAG
410
ALGAERKRAT PGRFGSVEK
Length:419
Mass (Da):46,340
Last modified:June 21, 2004 - v2
Checksum:i7C85AB6C38925145
GO

Sequence cautioni

The sequence AAA32850 differs from that shown.Sequencing errors.Curated
The sequence AAA32850 differs from that shown. Reason: Frameshift at several positions. Curated
The sequence CAA20030 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB80276 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63234 mRNA. Translation: AAA32850.1. Sequence problems.
AL031135 Genomic DNA. Translation: CAA20030.1. Sequence problems.
AL161587 Genomic DNA. Translation: CAB80276.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86538.1.
AY065403 mRNA. Translation: AAL38844.1.
AY096501 mRNA. Translation: AAM20151.1.
PIRiA39357.
C85420.
T04665.
RefSeqiNP_195285.3. NM_119725.4. [P27450-1]
UniGeneiAt.57035.

Genome annotation databases

EnsemblPlantsiAT4G35600.1; AT4G35600.1; AT4G35600. [P27450-1]
GeneIDi829712.
KEGGiath:AT4G35600.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63234 mRNA. Translation: AAA32850.1. Sequence problems.
AL031135 Genomic DNA. Translation: CAA20030.1. Sequence problems.
AL161587 Genomic DNA. Translation: CAB80276.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86538.1.
AY065403 mRNA. Translation: AAL38844.1.
AY096501 mRNA. Translation: AAM20151.1.
PIRiA39357.
C85420.
T04665.
RefSeqiNP_195285.3. NM_119725.4. [P27450-1]
UniGeneiAt.57035.

3D structure databases

ProteinModelPortaliP27450.
SMRiP27450. Positions 21-391.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14994. 3 interactions.
IntActiP27450. 8 interactions.
STRINGi3702.AT4G35600.2.

PTM databases

iPTMnetiP27450.

Proteomic databases

PaxDbiP27450.
PRIDEiP27450.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G35600.1; AT4G35600.1; AT4G35600. [P27450-1]
GeneIDi829712.
KEGGiath:AT4G35600.

Organism-specific databases

TAIRiAT4G35600.

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiP27450.
KOiK00924.
PhylomeDBiP27450.

Enzyme and pathway databases

BioCyciARA:AT4G35600-MONOMER.
ARA:GQT-2693-MONOMER.

Miscellaneous databases

PROiP27450.

Gene expression databases

ExpressionAtlasiP27450. baseline and differential.
GenevisibleiP27450. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCX32_ARATH
AccessioniPrimary (citable) accession number: P27450
Secondary accession number(s): O81792, Q8VZ07, Q9M068
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: June 21, 2004
Last modified: June 8, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was originally (PubMed:1851993) reported to be a connexin and to contain transmembrane domains. PubMed:8400879 authors have assigned that this is not a connexin, but rather a protein kinase.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.