Reviewed, UniProtKB/Swiss-Prot P27450 (CX32_ARATH)

Names and origin

Protein names

Recommended name:
    Probable serine/threonine-protein kinase Cx32, chloroplastic
    EC=2.7.11.1

Gene names

Ordered Locus Names:	At4g35600
ORF Names:	F8D20.110

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Caution	Was originally (Ref.1) reported to be a connexin and to contain transmembrane domains. Ref.4 authors have assigned that this is not a connexin, but rather a protein kinase.
Sequence caution	The sequence AAA32850.1 differs from that shown. Reason: Frameshift at several positions. The sequence AAA32850.1 differs from that shown. Reason: Miscellaneous discrepancy. Sequencing errors. The sequence CAA20030.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB80276.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q2V412	1	EBI-1238108,EBI-1238781
CALM	P62157	1	EBI-1238108,EBI-397403	From a different organism.
CAM1	P25854	1	EBI-1238108,EBI-1235664
CAM6	Q03509	1	EBI-1238108,EBI-1236097
CAM7	P59220	1	EBI-1238108,EBI-1236031
CML10	P30187	1	EBI-1238108,EBI-1238722
CML8	O23320	1	EBI-1238108,EBI-1237764
CML9	Q9S744	1	EBI-1238108,EBI-1236048

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 42	42	Chloroplast Potential
	Chain	43 – 419	377	Probable serine/threonine-protein kinase Cx32, chloroplastic		PRO_0000024321
Regions
	Domain	86 – 368	283	Protein kinase
	Nucleotide binding	92 – 100	9	ATP By similarity
	Compositional bias	9 – 14	6	Poly-Ser
Sites
	Active site	218	1	Proton acceptor By similarity
	Binding site	124	1	ATP By similarity
Amino acid modifications
	Modified residue	117	1	Phosphoserine Ref.5 Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P27450-1 [UniParc]. Last modified June 21, 2004. Version 2. Checksum: 7C85AB6C38925145	FASTA	419	46,340
	10 20 30 40 50 60 MGACISFFSS SSPSKTGLHS HATTNNHSNG TEFSSTTGAT TNSSVGQQSQ FSDISTGIIS 70 80 90 100 110 120 DSGKLLESPN LKVYNFLDLK TATKNFKPDS MLGQGGFGKV YRGWVDATTL APSRVGSGMI 130 140 150 160 170 180 VAIKRLNSES VQGFAEWRSE VNFLGMLSHR NLVKLLGYCR EDKELLLVYE FMPKGSLESH 190 200 210 220 230 240 LFRRNDPFPW DLRIKIVIGA ARGLAFLHSL QREVIYRDFK ASNILLDSNY DAKLSDFGLA 250 260 270 280 290 300 KLGPADEKSH VTTRIMGTYG YAAPEYMATG HLYVKSDVFA FGVVLLEIMT GLTAHNTKRP 310 320 330 340 350 360 RGQESLVDWL RPELSNKHRV KQIMDKGIKG QYTTKVATEM ARITLSCIEP DPKNRPHMKE 370 380 390 400 410 VVEVLEHIQG LNVVPNRSST KQAVANSSRS SPHHYRYKAG ALGAERKRAT PGRFGSVEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gap junction protein homologue from Arabidopsis thaliana: evidence for connexins in plants." Meiners S., Xu A., Schindler M. Proc. Natl. Acad. Sci. U.S.A. 88:4119-4122(1991) [PubMed: 1851993] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The proposed plant connexin is a protein kinase-like protein." Mushegian A.R., Koonin E.V. Plant Cell 5:998-999(1993) [PubMed: 8400879] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
[5]	"Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database." Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C. Plant Cell 16:2394-2405(2004) [PubMed: 15308754] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117, MASS SPECTROMETRY.
[6]	"Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis." Niittylae T., Fuglsang A.T., Palmgren M.G., Frommer W.B., Schulze W.X. Mol. Cell. Proteomics 6:1711-1726(2007) [PubMed: 17586839] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63234 mRNA. Translation: AAA32850.1. Sequence problems. AL031135 Genomic DNA. Translation: CAA20030.1. Sequence problems. AL161587 Genomic DNA. Translation: CAB80276.1. Sequence problems. AY065403 mRNA. Translation: AAL38844.1. AY096501 mRNA. Translation: AAM20151.1.
IPI	IPI00549060.
PIR	A39357. C85420. T04665.
RefSeq	NP_195285.3.
UniGene	At.57035
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P27450. 8 interactions.
Protein family/group databases
TCDB	1.A.26.1.1. plant plasmodesmata (PPD) family.
Proteomic databases
ProMEX	P27450.
Genome annotation databases
GeneID	829712.
GenomeReviews	Gene locus AT4G35600 in contig CT486007_GR.
KEGG	ath:AT4G35600.
NMPDR	fig|3702.1.peg.21645.
Organism-specific databases
GeneFarm	1725. 129.
TAIR	At4g35600.
Phylogenomic databases
OMA	P27450. NCASAID.
Enzyme and pathway databases
BRENDA	2.7.11.1. 302.
Gene expression databases
ArrayExpress	P27450.
GermOnline	AT4G35600. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CX32_ARATH

Accession

Primary (citable) accession number: P27450
Secondary accession number(s): O81792, Q8VZ07, Q9M068

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	June 21, 2004
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)