Reviewed, UniProtKB/Swiss-Prot P27448 (MARK3_HUMAN)

Names and origin

Protein names

Recommended name:
    MAP/microtubule affinity-regulating kinase 3
    EC=2.7.11.1
Alternative name(s):
    Cdc25C-associated protein kinase 1
    C-TAK1
      Short name=cTAK1
    Serine/threonine-protein kinase p78
    Ser/Thr protein kinase PAR-1
    Protein kinase STK10

Gene names

Name:	MARK3
Synonyms:	CTAK1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on 'Ser-216'.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily. Contains 1 KA1 (kinase-associated) domain. Contains 1 protein kinase domain. Contains 1 UBA domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ASB13	Q8WXK3	1	EBI-707595,EBI-707573
TCEA2	Q15560	1	EBI-707595,EBI-710310
YWHAB	P31946	1	EBI-707595,EBI-359815
YWHAG	P61981	1	EBI-707595,EBI-359832
YWHAQ	P27348	1	EBI-707595,EBI-359854

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P27448-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P27448-2)

Also known as: CTAK75a;

The sequence of this isoform differs from the canonical sequence as follows:
     638-661: Missing.

Isoform 3 (identifier: P27448-3)

The sequence of this isoform differs from the canonical sequence as follows:
     162-184: Missing.
     638-661: Missing.

Isoform 4 (identifier: P27448-4)

The sequence of this isoform differs from the canonical sequence as follows:
     162-184: Missing.
     638-646: Missing.

Isoform 5 (identifier: P27448-5)

The sequence of this isoform differs from the canonical sequence as follows:
     162-184: Missing.

Isoform 6 (identifier: P27448-6)

Also known as: p58;

The sequence of this isoform differs from the canonical sequence as follows:
     162-184: Missing.
     394-409: Missing.
     638-661: Missing.

Isoform 7 (identifier: P27448-7)

The sequence of this isoform differs from the canonical sequence as follows:
     394-409: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 776	776	MAP/microtubule affinity-regulating kinase 3		PRO_0000086304
Regions
	Domain	56 – 330	275	Protein kinase
	Domain	326 – 365	40	UBA
	Domain	727 – 776	50	KA1
	Nucleotide binding	62 – 70	9	ATP By similarity
Sites
	Active site	201	1	Proton acceptor By similarity
	Binding site	85	1	ATP By similarity
Amino acid modifications
	Modified residue	61	1	Phosphothreonine Ref.10
	Modified residue	234	1	Phosphothreonine Ref.8
	Modified residue	403	1	Phosphoserine Ref.10
	Modified residue	406	1	Phosphoserine Ref.10
	Modified residue	423	1	Phosphoserine Ref.10
	Modified residue	442	1	Phosphoserine Ref.10 Ref.8
	Modified residue	478	1	Phosphoserine Ref.10
	Modified residue	492	1	Phosphoserine Ref.10 Ref.8
	Modified residue	499	1	Phosphoserine Ref.10
	Modified residue	559	1	Phosphothreonine Ref.10
	Modified residue	563	1	Phosphoserine Ref.9
	Modified residue	566	1	Phosphoserine Ref.9
	Modified residue	572	1	Phosphothreonine Ref.10 Ref.9
	Modified residue	606	1	Phosphoserine Ref.10
	Modified residue	610	1	Phosphoserine Ref.10
	Modified residue	614	1	Phosphothreonine Ref.9
	Modified residue	621	1	Phosphoserine Ref.10
	Modified residue	624	1	Phosphoserine Ref.8
	Modified residue	666	1	Phosphoserine Ref.10
Natural variations
	Alternative sequence	162 – 184	23	Missing in isoform 3, isoform 4, isoform 5 and isoform 6.		VSP_004942
	Alternative sequence	394 – 409	16	Missing in isoform 6 and isoform 7.		VSP_004943
	Alternative sequence	638 – 661	24	Missing in isoform 2, isoform 3 and isoform 6.		VSP_004944
	Alternative sequence	638 – 646	9	Missing in isoform 4.		VSP_004945
	Natural variant	452	1	V → A Ref.11		VAR_040765
	Natural variant	466	1	S → G Ref.11 Ref.1 Ref.2		VAR_046763
Experimental info
	Sequence conflict	125	1	E → Q in AAA59991. Ref.5
	Sequence conflict	139	1	E → K Ref.4
	Sequence conflict	139	1	E → K Ref.5
	Sequence conflict	149	1	R → K in AAD48007. Ref.2
	Sequence conflict	448	1	A → G in AAA59991. Ref.5
	Sequence conflict	479	1	S → T in AAA59991. Ref.5
	Sequence conflict	539	1	A → D in AAA59991. Ref.5
	Sequence conflict	626	1	N → T in AAA59991. Ref.5
	Sequence conflict	668	1	E → K in AAA59991. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 28, 2003. Version 2. Checksum: A245496849070098	FASTA	776	86,944
	10 20 30 40 50 60 MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ PHIGNYRLLK 70 80 90 100 110 120 TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL FREVRIMKIL NHPNIVKLFE 130 140 150 160 170 180 VIETEKTLYL IMEYASGGEV FDYLVAHGRM KEKEARSKFR QGCQAGQTIK VQVSFDLLSL 190 200 210 220 230 240 MFTFIVSAVQ YCHQKRIVHR DLKAENLLLD ADMNIKIADF GFSNEFTVGG KLDTFCGSPP 250 260 270 280 290 300 YAAPELFQGK KYDGPEVDVW SLGVILYTLV SGSLPFDGQN LKELRERVLR GKYRIPFYMS 310 320 330 340 350 360 TDCENLLKRF LVLNPIKRGT LEQIMKDRWI NAGHEEDELK PFVEPELDIS DQKRIDIMVG 370 380 390 400 410 420 MGYSQEEIQE SLSKMKYDEI TATYLLLGRK SSELDASDSS SSSNLSLAKV RPSSDLNNST 430 440 450 460 470 480 GQSPHHKVQR SVSSSQKQRR YSDHAGPAIP SVVAYPKRSQ TSTADSDLKE DGISSRKSSG 490 500 510 520 530 540 SAVGGKGIAP ASPMLGNASN PNKADIPERK KSSTVPSSNT ASGGMTRRNT YVCSERTTAD 550 560 570 580 590 600 RHSVIQNGKE NSTIPDQRTP VASTHSISSA ATPDRIRFPR GTASRSTFHG QPRERRTATY 610 620 630 640 650 660 NGPPASPSLS HEATPLSQTR SRGSTNLFSK LTSKLTRRNM SFRFIKRLPT EYERNGRYEG 670 680 690 700 710 720 SSRNVSAEQK DENKEAKPRS LRFTWSMKTT SSMDPGDMMR EIRKVLDANN CDYEQRERFL 730 740 750 760 770 LFCVHGDGHA ENLVQWEMEV CKLPRLSLNG VRFKRISGTS IAFKNIASKI ANELKL
	Isoform 2 (CTAK75a). Checksum: 4577DF508B021FAF	FASTA	752	83,955
	10 20 30 40 50 60 MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ PHIGNYRLLK 70 80 90 100 110 120 TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL FREVRIMKIL NHPNIVKLFE 130 140 150 160 170 180 VIETEKTLYL IMEYASGGEV FDYLVAHGRM KEKEARSKFR QGCQAGQTIK VQVSFDLLSL 190 200 210 220 230 240 MFTFIVSAVQ YCHQKRIVHR DLKAENLLLD ADMNIKIADF GFSNEFTVGG KLDTFCGSPP 250 260 270 280 290 300 YAAPELFQGK KYDGPEVDVW SLGVILYTLV SGSLPFDGQN LKELRERVLR GKYRIPFYMS 310 320 330 340 350 360 TDCENLLKRF LVLNPIKRGT LEQIMKDRWI NAGHEEDELK PFVEPELDIS DQKRIDIMVG 370 380 390 400 410 420 MGYSQEEIQE SLSKMKYDEI TATYLLLGRK SSELDASDSS SSSNLSLAKV RPSSDLNNST 430 440 450 460 470 480 GQSPHHKVQR SVSSSQKQRR YSDHAGPAIP SVVAYPKRSQ TSTADSDLKE DGISSRKSSG 490 500 510 520 530 540 SAVGGKGIAP ASPMLGNASN PNKADIPERK KSSTVPSSNT ASGGMTRRNT YVCSERTTAD 550 560 570 580 590 600 RHSVIQNGKE NSTIPDQRTP VASTHSISSA ATPDRIRFPR GTASRSTFHG QPRERRTATY 610 620 630 640 650 660 NGPPASPSLS HEATPLSQTR SRGSTNLFSK LTSKLTRSRN VSAEQKDENK EAKPRSLRFT 670 680 690 700 710 720 WSMKTTSSMD PGDMMREIRK VLDANNCDYE QRERFLLFCV HGDGHAENLV QWEMEVCKLP 730 740 750 RLSLNGVRFK RISGTSIAFK NIASKIANEL KL
	Isoform 3. Checksum: 0B808DC1BC333C25	FASTA	729	81,439
	10 20 30 40 50 60 MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ PHIGNYRLLK 70 80 90 100 110 120 TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL FREVRIMKIL NHPNIVKLFE 130 140 150 160 170 180 VIETEKTLYL IMEYASGGEV FDYLVAHGRM KEKEARSKFR QIVSAVQYCH QKRIVHRDLK 190 200 210 220 230 240 AENLLLDADM NIKIADFGFS NEFTVGGKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG 250 260 270 280 290 300 VILYTLVSGS LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPIKRGTLEQ 310 320 330 340 350 360 IMKDRWINAG HEEDELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS KMKYDEITAT 370 380 390 400 410 420 YLLLGRKSSE LDASDSSSSS NLSLAKVRPS SDLNNSTGQS PHHKVQRSVS SSQKQRRYSD 430 440 450 460 470 480 HAGPAIPSVV AYPKRSQTST ADSDLKEDGI SSRKSSGSAV GGKGIAPASP MLGNASNPNK 490 500 510 520 530 540 ADIPERKKSS TVPSSNTASG GMTRRNTYVC SERTTADRHS VIQNGKENST IPDQRTPVAS 550 560 570 580 590 600 THSISSAATP DRIRFPRGTA SRSTFHGQPR ERRTATYNGP PASPSLSHEA TPLSQTRSRG 610 620 630 640 650 660 STNLFSKLTS KLTRSRNVSA EQKDENKEAK PRSLRFTWSM KTTSSMDPGD MMREIRKVLD 670 680 690 700 710 720 ANNCDYEQRE RFLLFCVHGD GHAENLVQWE MEVCKLPRLS LNGVRFKRIS GTSIAFKNIA SKIANELKL
	Isoform 4. Checksum: 6FB18E6E4B830D9F	FASTA	744	83,248
	10 20 30 40 50 60 MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ PHIGNYRLLK 70 80 90 100 110 120 TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL FREVRIMKIL NHPNIVKLFE 130 140 150 160 170 180 VIETEKTLYL IMEYASGGEV FDYLVAHGRM KEKEARSKFR QIVSAVQYCH QKRIVHRDLK 190 200 210 220 230 240 AENLLLDADM NIKIADFGFS NEFTVGGKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG 250 260 270 280 290 300 VILYTLVSGS LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPIKRGTLEQ 310 320 330 340 350 360 IMKDRWINAG HEEDELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS KMKYDEITAT 370 380 390 400 410 420 YLLLGRKSSE LDASDSSSSS NLSLAKVRPS SDLNNSTGQS PHHKVQRSVS SSQKQRRYSD 430 440 450 460 470 480 HAGPAIPSVV AYPKRSQTST ADSDLKEDGI SSRKSSGSAV GGKGIAPASP MLGNASNPNK 490 500 510 520 530 540 ADIPERKKSS TVPSSNTASG GMTRRNTYVC SERTTADRHS VIQNGKENST IPDQRTPVAS 550 560 570 580 590 600 THSISSAATP DRIRFPRGTA SRSTFHGQPR ERRTATYNGP PASPSLSHEA TPLSQTRSRG 610 620 630 640 650 660 STNLFSKLTS KLTRRLPTEY ERNGRYEGSS RNVSAEQKDE NKEAKPRSLR FTWSMKTTSS 670 680 690 700 710 720 MDPGDMMREI RKVLDANNCD YEQRERFLLF CVHGDGHAEN LVQWEMEVCK LPRLSLNGVR 730 740 FKRISGTSIA FKNIASKIAN ELKL
	Isoform 5. Checksum: B50B3C145E896B0B	FASTA	753	84,429
	10 20 30 40 50 60 MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ PHIGNYRLLK 70 80 90 100 110 120 TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL FREVRIMKIL NHPNIVKLFE 130 140 150 160 170 180 VIETEKTLYL IMEYASGGEV FDYLVAHGRM KEKEARSKFR QIVSAVQYCH QKRIVHRDLK 190 200 210 220 230 240 AENLLLDADM NIKIADFGFS NEFTVGGKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG 250 260 270 280 290 300 VILYTLVSGS LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPIKRGTLEQ 310 320 330 340 350 360 IMKDRWINAG HEEDELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS KMKYDEITAT 370 380 390 400 410 420 YLLLGRKSSE LDASDSSSSS NLSLAKVRPS SDLNNSTGQS PHHKVQRSVS SSQKQRRYSD 430 440 450 460 470 480 HAGPAIPSVV AYPKRSQTST ADSDLKEDGI SSRKSSGSAV GGKGIAPASP MLGNASNPNK 490 500 510 520 530 540 ADIPERKKSS TVPSSNTASG GMTRRNTYVC SERTTADRHS VIQNGKENST IPDQRTPVAS 550 560 570 580 590 600 THSISSAATP DRIRFPRGTA SRSTFHGQPR ERRTATYNGP PASPSLSHEA TPLSQTRSRG 610 620 630 640 650 660 STNLFSKLTS KLTRRNMSFR FIKRLPTEYE RNGRYEGSSR NVSAEQKDEN KEAKPRSLRF 670 680 690 700 710 720 TWSMKTTSSM DPGDMMREIR KVLDANNCDY EQRERFLLFC VHGDGHAENL VQWEMEVCKL 730 740 750 PRLSLNGVRF KRISGTSIAF KNIASKIANE LKL
	Isoform 6 (p58). Checksum: 74C47366AA34FCF8	FASTA	713	79,876
	10 20 30 40 50 60 MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ PHIGNYRLLK 70 80 90 100 110 120 TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL FREVRIMKIL NHPNIVKLFE 130 140 150 160 170 180 VIETEKTLYL IMEYASGGEV FDYLVAHGRM KEKEARSKFR QIVSAVQYCH QKRIVHRDLK 190 200 210 220 230 240 AENLLLDADM NIKIADFGFS NEFTVGGKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG 250 260 270 280 290 300 VILYTLVSGS LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPIKRGTLEQ 310 320 330 340 350 360 IMKDRWINAG HEEDELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS KMKYDEITAT 370 380 390 400 410 420 YLLLGRKSSE VRPSSDLNNS TGQSPHHKVQ RSVSSSQKQR RYSDHAGPAI PSVVAYPKRS 430 440 450 460 470 480 QTSTADSDLK EDGISSRKSS GSAVGGKGIA PASPMLGNAS NPNKADIPER KKSSTVPSSN 490 500 510 520 530 540 TASGGMTRRN TYVCSERTTA DRHSVIQNGK ENSTIPDQRT PVASTHSISS AATPDRIRFP 550 560 570 580 590 600 RGTASRSTFH GQPRERRTAT YNGPPASPSL SHEATPLSQT RSRGSTNLFS KLTSKLTRSR 610 620 630 640 650 660 NVSAEQKDEN KEAKPRSLRF TWSMKTTSSM DPGDMMREIR KVLDANNCDY EQRERFLLFC 670 680 690 700 710 VHGDGHAENL VQWEMEVCKL PRLSLNGVRF KRISGTSIAF KNIASKIANE LKL
	Isoform 7. Checksum: F5148E18A88C54B7	FASTA	760	85,381
	10 20 30 40 50 60 MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ PHIGNYRLLK 70 80 90 100 110 120 TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL FREVRIMKIL NHPNIVKLFE 130 140 150 160 170 180 VIETEKTLYL IMEYASGGEV FDYLVAHGRM KEKEARSKFR QGCQAGQTIK VQVSFDLLSL 190 200 210 220 230 240 MFTFIVSAVQ YCHQKRIVHR DLKAENLLLD ADMNIKIADF GFSNEFTVGG KLDTFCGSPP 250 260 270 280 290 300 YAAPELFQGK KYDGPEVDVW SLGVILYTLV SGSLPFDGQN LKELRERVLR GKYRIPFYMS 310 320 330 340 350 360 TDCENLLKRF LVLNPIKRGT LEQIMKDRWI NAGHEEDELK PFVEPELDIS DQKRIDIMVG 370 380 390 400 410 420 MGYSQEEIQE SLSKMKYDEI TATYLLLGRK SSEVRPSSDL NNSTGQSPHH KVQRSVSSSQ 430 440 450 460 470 480 KQRRYSDHAG PAIPSVVAYP KRSQTSTADS DLKEDGISSR KSSGSAVGGK GIAPASPMLG 490 500 510 520 530 540 NASNPNKADI PERKKSSTVP SSNTASGGMT RRNTYVCSER TTADRHSVIQ NGKENSTIPD 550 560 570 580 590 600 QRTPVASTHS ISSAATPDRI RFPRGTASRS TFHGQPRERR TATYNGPPAS PSLSHEATPL 610 620 630 640 650 660 SQTRSRGSTN LFSKLTSKLT RRNMSFRFIK RLPTEYERNG RYEGSSRNVS AEQKDENKEA 670 680 690 700 710 720 KPRSLRFTWS MKTTSSMDPG DMMREIRKVL DANNCDYEQR ERFLLFCVHG DGHAENLVQW 730 740 750 760 EMEVCKLPRL SLNGVRFKRI SGTSIAFKNI ASKIANELKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"C-TAK1 protein kinase phosphorylates human Cdc25C on serine 216 and promotes 14-3-3 protein binding." Peng C.Y., Graves P.R., Ogg S., Thoma R.S., Byrnes M.J. III, Wu Z., Stephenson M.T., Piwnica-Worms H. Cell Growth Differ. 9:197-208(1998) [PubMed: 9543386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), VARIANT GLY-466.
[2]	"Human serine/threonine protein kinase cTAK1/Kp78/Mark3: Identification of a novel splice variant and a larger 5'UTR." Waggoner S.N., Stephen R., Farrar W.L., Howard O.M.Z. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT GLY-466.
[3]	"PAR-1 is a Dishevelled-associated kinase and a positive regulator of Wnt signalling." Sun T.-Q., Lu B., Feng J.-J., Reinhard C., Jan Y.N., Fantl W.J., Williams L.T. Nat. Cell Biol. 3:628-636(2001) [PubMed: 11433294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
[4]	"Characterization of an alternatively spliced form of MARK3 from human brain." Drewes G. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
[5]	Maheshwari K.K., Som S., Parsa I. Submitted (JAN-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6).
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Cervix.
[7]	"Homo sapiens mRNA partial sequence for a protein kinase, STK10, similar to p78/C-TAK1." Reynolds C.H., Patel U.A., Anderton B.H. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 323-775 (ISOFORM 7). Tissue: Urinary bladder.
[8]	"Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry." Wissing J., Jaensch L., Nimtz M., Dieterich G., Hornberger R., Keri G., Wehland J., Daub H. Mol. Cell. Proteomics 6:537-547(2007) [PubMed: 17192257] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-234; SER-442; SER-492 AND SER-624, MASS SPECTROMETRY.
[9]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-563; SER-566; THR-572 AND THR-614, MASS SPECTROMETRY.
[10]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-61; SER-403; SER-406; SER-423; SER-442; SER-478; SER-492; SER-499; THR-559; THR-572; SER-606; SER-610; SER-621 AND SER-666, MASS SPECTROMETRY.
[11]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] ALA-452 AND GLY-466.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U64205 mRNA. Translation: AAC15093.1.
AF159295 mRNA. Translation: AAD48007.1.
AF387637 mRNA. Translation: AAK82367.1.
AF465413 mRNA. Translation: AAL69982.1.
M80359 mRNA. Translation: AAA59991.1.
BC024773 mRNA. Translation: AAH24773.1.
AF170723 mRNA. Translation: AAD51631.1.

IPI

IPI00183118.
IPI00220505.
IPI00220506.
IPI00220507.
IPI00220508.
IPI00220509.
IPI00220510.

PIR

S27966.

RefSeq

NP_001122390.1.
NP_001122391.1.
NP_001122392.1.
NP_002367.4.

UniGene

Hs.35828

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QNJ	X-ray	2.70	A/B	48-393	[»]
3FE3	X-ray	1.90	A/B	41-390	[»]

SMR

P27448. Positions 680-776.

ModBase

Search...

Protein-protein interaction databases

IntAct

P27448. 22 interactions.

PTM databases

PhosphoSite

P27448.

Proteomic databases

PRIDE

P27448.

Genome annotation databases

Ensembl

ENSG00000075413. Homo sapiens. [Contig view]

GeneID

4140.

KEGG

hsa:4140.

Organism-specific databases

GeneCards

GC14P102921.

H-InvDB

HIX0012000.

HGNC

HGNC:6897. MARK3.

MIM

602678. gene.

PharmGKB

PA30640.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P27448.

Enzyme and pathway databases

BRENDA

2.7.11.1. 247.

Gene expression databases

ArrayExpress

P27448.

Bgee

P27448.

CleanEx

HS_MARK3.

GermOnline

ENSG00000075413. Homo sapiens.

Family and domain databases

InterPro

IPR001772. Kinase-assoc_KA1.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
IPR000449. UBA/transl_elong_EF1B_N.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view]

Pfam

PF02149. KA1. 1 hit.
PF00069. Pkinase. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]

ProDom

PD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00220. S_TKc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]

PROSITE

PS50032. KA1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

MARK3_HUMAN

Accession

Primary (citable) accession number: P27448
Secondary accession number(s): O60219 , Q8TB41, Q8WX83, Q96RG1, Q9UMY9, Q9UN34

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	February 28, 2003
Last modified:	June 16, 2009
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)