Reviewed,
UniProtKB/Swiss-Prot P27448 (MARK3_HUMAN)
Last modified
June 16, 2009.
Version 101.
History...
Clusters with 100%,
90%,
50% identity |
Documents (7) |
Third-party data |
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Names and origin
| Protein names | Recommended name: MAP/microtubule affinity-regulating kinase 3 EC=2.7.11.1 Alternative name(s): Cdc25C-associated protein kinase 1 C-TAK1 Short name=cTAK1 Serine/threonine-protein kinase p78 Ser/Thr protein kinase PAR-1 Protein kinase STK10 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 776 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on 'Ser-216'. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Tissue specificity | Ubiquitous. |
| Sequence similarities | Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily. Contains 1 KA1 (kinase-associated) domain. Contains 1 protein kinase domain. Contains 1 UBA domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing Polymorphism |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Technical term | 3D-structure |
| Gene Ontology (GO) | |
| Biological process | protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from physical interaction. Source: IntAct protein serine/threonine kinase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ASB13 | Q8WXK3 | 1 | EBI-707595,EBI-707573 | |
| TCEA2 | Q15560 | 1 | EBI-707595,EBI-710310 | |
| YWHAB | P31946 | 1 | EBI-707595,EBI-359815 | |
| YWHAG | P61981 | 1 | EBI-707595,EBI-359832 | |
| YWHAQ | P27348 | 1 | EBI-707595,EBI-359854 |
Alternative products
| This entry describes 7 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P27448-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P27448-2) Also known as: CTAK75a; The sequence of this isoform differs from the canonical sequence as follows: 638-661: Missing. | ||||||
| Isoform 3 (identifier: P27448-3) The sequence of this isoform differs from the canonical sequence as follows: 162-184: Missing. 638-661: Missing. | ||||||
| Isoform 4 (identifier: P27448-4) The sequence of this isoform differs from the canonical sequence as follows: 162-184: Missing. 638-646: Missing. | ||||||
| Isoform 5 (identifier: P27448-5) The sequence of this isoform differs from the canonical sequence as follows: 162-184: Missing. | ||||||
| Isoform 6 (identifier: P27448-6) Also known as: p58; The sequence of this isoform differs from the canonical sequence as follows: 162-184: Missing. 394-409: Missing. 638-661: Missing. | ||||||
| Isoform 7 (identifier: P27448-7) The sequence of this isoform differs from the canonical sequence as follows: 394-409: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 776 | 776 | MAP/microtubule affinity-regulating kinase 3 | PRO_0000086304 | |||||
Regions | |||||||||
| Domain | 56 – 330 | 275 | Protein kinase | ||||||
| Domain | 326 – 365 | 40 | UBA | ||||||
| Domain | 727 – 776 | 50 | KA1 | ||||||
| Nucleotide binding | 62 – 70 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 201 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 85 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 61 | 1 | Phosphothreonine Ref.10 | ||||||
| Modified residue | 234 | 1 | Phosphothreonine Ref.8 | ||||||
| Modified residue | 403 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 406 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 423 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 442 | 1 | Phosphoserine Ref.10 Ref.8 | ||||||
| Modified residue | 478 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 492 | 1 | Phosphoserine Ref.10 Ref.8 | ||||||
| Modified residue | 499 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 559 | 1 | Phosphothreonine Ref.10 | ||||||
| Modified residue | 563 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 566 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 572 | 1 | Phosphothreonine Ref.10 Ref.9 | ||||||
| Modified residue | 606 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 610 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 614 | 1 | Phosphothreonine Ref.9 | ||||||
| Modified residue | 621 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 624 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 666 | 1 | Phosphoserine Ref.10 | ||||||
Natural variations | |||||||||
| Alternative sequence | 162 – 184 | 23 | Missing in isoform 3, isoform 4, isoform 5 and isoform 6. | VSP_004942 | |||||
| Alternative sequence | 394 – 409 | 16 | Missing in isoform 6 and isoform 7. | VSP_004943 | |||||
| Alternative sequence | 638 – 661 | 24 | Missing in isoform 2, isoform 3 and isoform 6. | VSP_004944 | |||||
| Alternative sequence | 638 – 646 | 9 | Missing in isoform 4. | VSP_004945 | |||||
| Natural variant | 452 | 1 | V → A Ref.11 | VAR_040765 | |||||
| Natural variant | 466 | 1 | S → G Ref.11 Ref.1 Ref.2 | VAR_046763 | |||||
Experimental info | |||||||||
| Sequence conflict | 125 | 1 | E → Q in AAA59991. Ref.5 | ||||||
| Sequence conflict | 139 | 1 | E → K Ref.4 | ||||||
| Sequence conflict | 139 | 1 | E → K Ref.5 | ||||||
| Sequence conflict | 149 | 1 | R → K in AAD48007. Ref.2 | ||||||
| Sequence conflict | 448 | 1 | A → G in AAA59991. Ref.5 | ||||||
| Sequence conflict | 479 | 1 | S → T in AAA59991. Ref.5 | ||||||
| Sequence conflict | 539 | 1 | A → D in AAA59991. Ref.5 | ||||||
| Sequence conflict | 626 | 1 | N → T in AAA59991. Ref.5 | ||||||
| Sequence conflict | 668 | 1 | E → K in AAA59991. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "C-TAK1 protein kinase phosphorylates human Cdc25C on serine 216 and promotes 14-3-3 protein binding." Peng C.Y., Graves P.R., Ogg S., Thoma R.S., Byrnes M.J. III, Wu Z., Stephenson M.T., Piwnica-Worms H. Cell Growth Differ. 9:197-208(1998) [PubMed: 9543386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), VARIANT GLY-466. |
| [2] | "Human serine/threonine protein kinase cTAK1/Kp78/Mark3: Identification of a novel splice variant and a larger 5'UTR." Waggoner S.N., Stephen R., Farrar W.L., Howard O.M.Z. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT GLY-466. |
| [3] | "PAR-1 is a Dishevelled-associated kinase and a positive regulator of Wnt signalling." Sun T.-Q., Lu B., Feng J.-J., Reinhard C., Jan Y.N., Fantl W.J., Williams L.T. Nat. Cell Biol. 3:628-636(2001) [PubMed: 11433294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4). |
| [4] | "Characterization of an alternatively spliced form of MARK3 from human brain." Drewes G. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5). |
| [5] | Maheshwari K.K., Som S., Parsa I. Submitted (JAN-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6). |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Cervix. |
| [7] | "Homo sapiens mRNA partial sequence for a protein kinase, STK10, similar to p78/C-TAK1." Reynolds C.H., Patel U.A., Anderton B.H. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 323-775 (ISOFORM 7). Tissue: Urinary bladder. |
| [8] | "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry." Wissing J., Jaensch L., Nimtz M., Dieterich G., Hornberger R., Keri G., Wehland J., Daub H. Mol. Cell. Proteomics 6:537-547(2007) [PubMed: 17192257] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-234; SER-442; SER-492 AND SER-624, MASS SPECTROMETRY. |
| [9] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-563; SER-566; THR-572 AND THR-614, MASS SPECTROMETRY. |
| [10] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-61; SER-403; SER-406; SER-423; SER-442; SER-478; SER-492; SER-499; THR-559; THR-572; SER-606; SER-610; SER-621 AND SER-666, MASS SPECTROMETRY. |
| [11] | "Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. Stratton M.R.Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] ALA-452 AND GLY-466. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| U64205 mRNA. Translation: AAC15093.1. AF159295 mRNA. Translation: AAD48007.1. AF387637 mRNA. Translation: AAK82367.1. AF465413 mRNA. Translation: AAL69982.1. M80359 mRNA. Translation: AAA59991.1. BC024773 mRNA. Translation: AAH24773.1. AF170723 mRNA. Translation: AAD51631.1. | |||||||||||||||||||
| IPI | IPI00183118. IPI00220505. IPI00220506. IPI00220507. IPI00220508. IPI00220509. IPI00220510. | ||||||||||||||||||
| PIR | S27966. | ||||||||||||||||||
| RefSeq | NP_001122390.1. NP_001122391.1. NP_001122392.1. NP_002367.4. | ||||||||||||||||||
| UniGene | Hs.35828 | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| |||||||||||||||||||
| SMR | P27448. Positions 680-776. | ||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| IntAct | P27448. 22 interactions. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | P27448. | ||||||||||||||||||
Proteomic databases | |||||||||||||||||||
| PRIDE | P27448. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENSG00000075413. Homo sapiens. [Contig view] | ||||||||||||||||||
| GeneID | 4140. | ||||||||||||||||||
| KEGG | hsa:4140. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| GeneCards | GC14P102921. | ||||||||||||||||||
| H-InvDB | HIX0012000. | ||||||||||||||||||
| HGNC | HGNC:6897. MARK3. | ||||||||||||||||||
| MIM | 602678. gene. | ||||||||||||||||||
| PharmGKB | PA30640. | ||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| HOVERGEN | P27448. | ||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||
| BRENDA | 2.7.11.1. 247. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | P27448. | ||||||||||||||||||
| Bgee | P27448. | ||||||||||||||||||
| CleanEx | HS_MARK3. | ||||||||||||||||||
| GermOnline | ENSG00000075413. Homo sapiens. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR001772. Kinase-assoc_KA1. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. IPR000449. UBA/transl_elong_EF1B_N. IPR015940. UBA/transl_elong_EF1B_N_euk. [Graphical view] | ||||||||||||||||||
| Pfam | PF02149. KA1. 1 hit. PF00069. Pkinase. 1 hit. PF00627. UBA. 1 hit. [Graphical view] | ||||||||||||||||||
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||||||||
| SMART | SM00220. S_TKc. 1 hit. SM00165. UBA. 1 hit. [Graphical view] | ||||||||||||||||||
| PROSITE | PS50032. KA1. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50030. UBA. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other Resources | |||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||
Entry information
| Entry name | MARK3_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P27448 Secondary accession number(s): O60219 Q9UN34 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 14 Human chromosome 14: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


