Reviewed, UniProtKB/Swiss-Prot P27446 (FYN_XIPHE)

Names and origin

Protein names

Recommended name:
    Proto-oncogene tyrosine-protein kinase FYN
    EC=2.7.10.2
Alternative name(s):
    P59-FYN

Gene names

Name:

fyn

Organism

Xiphophorus helleri (Green swordtail)

Taxonomic identifier

8084 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Atherinomorpha › Cyprinodontiformes › Poeciliidae › Poeciliinae › Xiphophorus

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Cofactor	Manganese.
Enzyme regulation	Inhibited by phosphorylation of Tyr-531 by leukocyte common antigen and activated by dephosphorylation of this site By similarity.
Subunit structure	Associates through its SH3 domain, to the p85 subunit of phosphatidylinositol 3-kinase.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Disease	Proto-oncogene
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Manganese Metal-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 537	536	Proto-oncogene tyrosine-protein kinase FYN		PRO_0000088102
Regions
	Domain	82 – 143	62	SH3
	Domain	149 – 246	98	SH2
	Domain	271 – 524	254	Protein kinase
	Nucleotide binding	277 – 285	9	ATP By similarity
Sites
	Active site	390	1	Proton acceptor By similarity
	Binding site	299	1	ATP By similarity
Amino acid modifications
	Modified residue	12	1	Phosphothreonine; by PKC By similarity
	Modified residue	420	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	531	1	Phosphotyrosine By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity
	Lipidation	3	1	S-palmitoyl cysteine By similarity
	Lipidation	6	1	S-palmitoyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27446-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 6AC5486B13C3876B	FASTA	537	60,447
	10 20 30 40 50 60 MGCVQCKDKE ATKLTDDRDA SISQGAGYRY GADPTPQHYP SFGVTAIPNY NNFHAPVGQG 70 80 90 100 110 120 VTVFGGVNTS SHTGTLRTRG GTGVTLFVAL YDYEARTEDD LSFRKGERFQ ILNSTEGDWW 130 140 150 160 170 180 DARSLTTGGS GYIPSNYVAP VDSIQAEDWY FGKLGRKDAE RQLLSTGNPR GTYLIRESET 190 200 210 220 230 240 TKGAFSLSIR DWDDEKGDHV KHYKIRKLDS GGYYITTRAQ FDTLQQLVQH YSDRAAGLCC 250 260 270 280 290 300 RLVVPCHKGM PRLADLSVKT KDVWEIPRES LQLIKRLGNG QFGEVWMGTW NGTTKVAVKT 310 320 330 340 350 360 LKPGTMSPES FLEEAQIMKK LRHDKLVQLY AVVSEEPIYI VTEYMSKGSL LDFLKDGEGR 370 380 390 400 410 420 ALKLPNLVDM AAQVAAGMAY IERMNYIHRD LRSANILVGD NLVCKIADFG LARLIEDNEY 430 440 450 460 470 480 TARQGAKFPI KWTAPEAALY GRFTIKSDVW SFGILLTELV TKGRVPYPGM NNREVLEQVE 490 500 510 520 530 RGYRMPCPQD CPASLHELML QCWKKDPEER PTFEYLQAFL EDYFTATEPQ YQPGDNL

References

[1]	"Conservation of structure and expression of the c-yes and fyn genes in lower vertebrates." Hannig G., Ottilie S., Schartl M. Oncogene 6:361-369(1991) [PubMed: 1707152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Rio Lancetilla.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54971 mRNA. Translation: CAA38715.1.
PIR	I51592.
3D structure databases
HSSP	HSSP built from PDB template 1G83 based on UniProtKB P06241.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P27446.
Enzyme and pathway databases
BRENDA	2.7.10.2. 262607.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001452. SH3_domain. IPR020473. SH3_region. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. PD000093. SH2. 1 hit. PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FYN_XIPHE

Accession

Primary (citable) accession number: P27446

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	September 22, 2009
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)