P27437 (CAPSD_MEVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Capsid protein VP1 Alternative name(s): Coat protein VP1 Cleaved into the following chain: Capsid protein VP2 Alternative name(s): Coat protein VP2
Organism	Mink enteritis virus (strain Abashiri) (MEV) [Complete proteome]
Taxonomic identifier	10793 [NCBI]
Taxonomic lineage	Viruses › ssDNA viruses › Parvoviridae › Parvovirinae › Parvovirus
Virus host	Mustela vison (American mink) (Neovison vison) [TaxID: 452646]

Protein attributes

Sequence length	722 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus By similarity.
Subcellular location	Capsid protein VP1: Virion Potential. Capsid protein VP2: Virion Potential.
Domain	The N-terminus of VP1 is sequestered within the mature capsid. It contains a phospholipase A2-like region and putative nuclear localization signals.
Sequence similarities	Belongs to the parvoviridae capsid protein family.

Ontologies

Keywords
Biological process	Clathrin-mediated endocytosis of virus by host Host-virus interaction Initiation of viral infection Viral attachment to host cell Viral penetration into host cytoplasm Viral penetration via permeabilization of host organellar membrane Virus endocytosis by host
Cellular component	Virion
Molecular function	Capsid protein T=1 icosahedral capsid protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 722	722	Capsid protein VP1		PRO_0000039415
	Chain	139 – 722	584	Capsid protein VP2		PRO_0000039416
Regions
	Region	14 – 59	46	Phospholipase A2-like By similarity
	Compositional bias	160 – 177	18	Gly-rich

Sequences

	Sequence		Length	Mass (Da)
	P27437 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 9DADBCAB2EF9F622	FASTA	722	79,823
	10 20 30 40 50 60 MCFFIGLVPP GYKYLGPGNS LDQGEPTNPS DAAAKEHDEA YAAYLRSGKN PYLYFSPADQ 70 80 90 100 110 120 RFIDQTKDAT DWGGKIGHYF FRAKKAIAPV LTDTPDHPST SRPTKPTKRS KPPPHIFINL 130 140 150 160 170 180 AKKKKAGAGQ VKRDNLAPMS DGAVQPDGGQ PAVRNERATG SGNGSGGGGG GGSGGVGIST 190 200 210 220 230 240 GTFNNQTEFK FLENGWVEIT ANSSRLVHLN MPESENYKRV VVNNMDKTAV KGNMALDDTH 250 260 270 280 290 300 VQIVTPWSLV DANAWGVWFN PGDWQLIVNT MSELHLVSFE QEIFNVVLKT VSESATQPPT 310 320 330 340 350 360 KVYNNDLTAS LMVALDSNNT MPFTPAAMRS ETLGFYPWKP TIPTPWRYYF QWDRTLIPSH 370 380 390 400 410 420 TGTSGTPTNI YHGTDPDDVQ FYTIENSVPV HLLRTGDEFA TGTFFFDCKP CRLTHTWQTN 430 440 450 460 470 480 RALGLPPFLN SLPQSEGATN FGDIGVQQDK RRGVTQMGNT DYITEATIMR PAEVGYSAPY 490 500 510 520 530 540 YSFEASTQGP FKTPIAAGRG GAQTDENQAA DGDPRYAFGR QHGQKTTTTG ETPERFTYIA 550 560 570 580 590 600 HQDTGRYPAG DWIQNINFNL PVTNDNVLLP TDPIGGKTGI NYTNIFNTYG PLTALNNVPP 610 620 630 640 650 660 VYPNGQIWDK EFDTDLKPRL HVNAPFVCQN NCPGQLFVKV APNLTNEYDP DASANMSRIV 670 680 690 700 710 720 TYSDFWWKGK LVFKAKLRAS HTWNPIQQMS INVDNQFNYL PNNIGAMKIV YEKSQLAPRK LY

References

[1]

"Construction and nucleotide sequence analysis of an infectious DNA clone of the autonomous parvovirus, mink enteritis virus." Kariatsumari T., Horiuchi M., Hama E., Yaguchi K., Ishigurio N., Goto H., Shinagawa M. J. Gen. Virol. 72:867-875(1991) [PubMed: 2016597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00765 Genomic DNA. Translation: BAA00663.1.
PIR	VCPVME. B38350.
3D structure databases
ProteinModelPortal	P27437.
SMR	P27437. Positions 175-722.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016184. Capsid/spike_ssDNA_virus. IPR001403. Parvovirus_coat. IPR013607. Parvovirus_coat_VP1_N. [Graphical view]
Gene3D	G3DSA:2.170.30.10. Parvo_coat. 1 hit.
Pfam	PF00740. Parvo_coat. 1 hit. PF08398. Parvo_coat_N. 1 hit. [Graphical view]
SUPFAM	SSF88645. Capsid/spike_ssDNA_virus. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CAPSD_MEVA

Accession

Primary (citable) accession number: P27437

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	February 1, 1996
Last modified:	May 31, 2011
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program