P27435 (TRYB1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptase
EC=3.4.21.59

Alternative name(s):
Mast cell protease 7
Short name=rMCP-7
Tryptase alpha/beta-1
Tryptase, skin

Gene names

Name:	Tpsab1
Synonyms:	Mcp7, Mcpt7, Tpsb1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.
Subunit structure	Homotetramer.
Subcellular location	Secreted. Note: Released from the secretory granules upon mast cell activation.
Tissue specificity	Mast cells.
Post-translational modification	Glycosylated Probable.
Sequence similarities	Belongs to the peptidase S1 family. Tryptase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 28	10	Activation peptide		PRO_0000027496
	Chain	29 – 273	245	Tryptase		PRO_0000027497
Regions
	Domain	29 – 270	242	Peptidase S1
Sites
	Active site	72	1	Charge relay system By similarity
	Active site	119	1	Charge relay system By similarity
	Active site	222	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	49	1	N-linked (GlcNAc...) Probable
	Disulfide bond	57 ↔ 73		By similarity
	Disulfide bond	153 ↔ 228		By similarity
	Disulfide bond	186 ↔ 209		By similarity
	Disulfide bond	218 ↔ 246		By similarity
Experimental info
	Sequence conflict	42	1	W → V AA sequence Ref.3
	Sequence conflict	49 – 51	3	NDT → WLP AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P27435 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 65A5ED4D279FB284	FASTA	273	30,400
	10 20 30 40 50 60 MLKLLLLTLP LLSSLVHAAP SLAMPREGIV GGQEASGNKW PWQVSLRVND TYWMHFCGGS 70 80 90 100 110 120 LIHPQWVLTA AHCVGPNKAD PNKLRVQLRK QYLYYHDHLL TVSQIISHPD FYIAQDGADI 130 140 150 160 170 180 ALLKLTNPVN ITSNVHTVSL PPASETFPSG TLCWVTGWGN INNDVSLPPP FPLEEVQVPI 190 200 210 220 230 240 VENRLCDLKY HKGLNTGDNV HIVRDDMLCA GNEGHDSCQG DSGGPLVCKV EDTWLQAGVV 250 260 270 SWGEGCAQPN RPGIYTRVTY YLDWIYRYVP KYF

References

[1]	"Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations." Lutzelschwab C., Pejler G., Aveskogh M., Hellman L. J. Exp. Med. 185:13-29(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"Tryptase from rat skin: purification and properties." Braganza V.J., Simmons W.H. Biochemistry 30:4997-5007(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-53. Strain: Sprague-Dawley. Tissue: Skin.
[3]	"Separation, purification and N-terminal sequence analysis of a novel leupeptin-sensitive serine endopeptidase present in chemically induced rat mammary tumour." Eto I., Grubbs C.J. Biochem. J. 283:209-216(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-51. Tissue: Mammary carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U67910 mRNA. Translation: AAB48263.1.
IPI	IPI00196866.
PIR	A23698. S21275.
RefSeq	NP_062195.2. NM_019322.2.
UniGene	Rn.10699.
3D structure databases
ProteinModelPortal	P27435.
SMR	P27435. Positions 29-271.
ModBase	Search...
Protein family/group databases
MEROPS	S01.026.
Proteomic databases
PaxDb	P27435.
PRIDE	P27435.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	54271.
KEGG	rno:54271.
UCSC	RGD:3066. rat.
Organism-specific databases
CTD	7177.
RGD	3066. Tpsab1.
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P27435.
KO	K01340.
OrthoDB	EOG4P8FJQ.
Gene expression databases
ArrayExpress	P27435.
Genevestigator	P27435.
GermOnline	ENSRNOG00000024181. Rattus norvegicus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P27435.
ChEMBL	CHEMBL3320.
NextBio	610832.

Entry information

Entry name

TRYB1_RAT

Accession

Primary (citable) accession number: P27435
Secondary accession number(s): P27436

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program