Reviewed, UniProtKB/Swiss-Prot P27422 (DYR7_ECOLX)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase type 7
    EC=1.5.1.3
Alternative name(s):
    Dihydrofolate reductase type VII

Gene names

Name:

dhfrVII

Encoded on

Plasmid pLMO27 Ref.1
Plasmid IncP-beta R751 Ref.2
Plasmid pDGO100 Ref.3

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	Antibiotic resistance Methotrexate resistance One-carbon metabolism Trimethoprim resistance
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Plasmid Transposable element
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW response to methotrexate Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	Dihydrofolate reductase type 7		PRO_0000186424
Regions
	Domain	2 – 156	155	DHFR

Sequences

	Sequence		Length	Mass (Da)
	P27422-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: A7791CC9C7E029DE	FASTA	157	17,594
	10 20 30 40 50 60 MKISLISATS ENGVIGNGPD IPWSAKGEQL LFKALTYNQW LLVGRKTFDS MGVLPNRKYA 70 80 90 100 110 120 VVSRKGISSS NENVLVFPSI EIALQELSKI TDHLYVSGGG QIYNSLIEKA DIIHLSTVHV 130 140 150 EVEGDINFPK IPENFNLVFE QFFLSNINYT YQIWKKG

References

[1]	"Characterization of transposon Tn5086, carrying the site-specifically inserted gene dhfrVII mediating trimethoprim resistance." Sundstroem L., Swedberg G., Skoeld O. J. Bacteriol. 175:1796-1805(1993) [PubMed: 8383666] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Transposon: Tn5086.
[2]	"Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements." Raadstroem P., Skoeld O., Swedberg G., Flensburg J., Roy P.H., Sundstroem L. J. Bacteriol. 176:3257-3268(1994) [PubMed: 8195081] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Transposon: Tn5090.
[3]	"Plasmid pDGO100 contains a second integron with the trimethoprim resistance gene dfrA7 as the inserted cassette." Burnside J.M., Groot Obbink D.J. Plasmid 35:67-70(1996) [PubMed: 8693029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: VA292.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58425 Genomic DNA. Translation: CAA41326.1. U31119 Genomic DNA. Translation: AAB18756.1.
PIR	A47087.
3D structure databases
SMR	P27422. Positions 1-156.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.5.1.3. 246.
Family and domain databases
InterPro	IPR012259. DHFR. IPR017925. Dihydrofolate_reductase_CS. IPR001796. Dihydrofolate_reductase_dom. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR7_ECOLX

Accession

Primary (citable) accession number: P27422

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)