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P27422 (DYR7_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase type 7

EC=1.5.1.3
Alternative name(s):
Dihydrofolate reductase type VII
Gene names
Name:dhfrVII
Encoded onPlasmid pLMO27 Ref.1
Plasmid IncP-beta R751 Ref.2
Plasmid pDGO100 Ref.3
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length157 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 157157Dihydrofolate reductase type 7
PRO_0000186424

Regions

Domain2 – 156155DHFR

Sequences

Sequence LengthMass (Da)Tools
P27422 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: A7791CC9C7E029DE

FASTA15717,594
        10         20         30         40         50         60 
MKISLISATS ENGVIGNGPD IPWSAKGEQL LFKALTYNQW LLVGRKTFDS MGVLPNRKYA 

        70         80         90        100        110        120 
VVSRKGISSS NENVLVFPSI EIALQELSKI TDHLYVSGGG QIYNSLIEKA DIIHLSTVHV 

       130        140        150 
EVEGDINFPK IPENFNLVFE QFFLSNINYT YQIWKKG 

« Hide

References

[1]"Characterization of transposon Tn5086, carrying the site-specifically inserted gene dhfrVII mediating trimethoprim resistance."
Sundstroem L., Swedberg G., Skoeld O.
J. Bacteriol. 175:1796-1805(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Transposon: Tn5086.
[2]"Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements."
Raadstroem P., Skoeld O., Swedberg G., Flensburg J., Roy P.H., Sundstroem L.
J. Bacteriol. 176:3257-3268(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Transposon: Tn5090.
[3]"Plasmid pDGO100 contains a second integron with the trimethoprim resistance gene dfrA7 as the inserted cassette."
Burnside J.M., Groot Obbink D.J.
Plasmid 35:67-70(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: VA292.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X58425 Genomic DNA. Translation: CAA41326.1.
U31119 Genomic DNA. Translation: AAB18756.1.
PIRA47087.
RefSeqYP_006953993.1. NC_019091.1.

3D structure databases

ProteinModelPortalP27422.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID13909542.

Phylogenomic databases

OMATEIHAIC.

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR7_ECOLX
AccessionPrimary (citable) accession number: P27422
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 16, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways