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P27422

- DYR7_ECOLX

UniProt

P27422 - DYR7_ECOLX

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Protein
Dihydrofolate reductase type 7
Gene
dhfrVII
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathwayi

GO - Molecular functioni

  1. NADP binding Source: InterPro
  2. dihydrofolate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. response to antibiotic Source: UniProtKB-KW
  5. response to methotrexate Source: UniProtKB-KW
  6. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, Methotrexate resistance, One-carbon metabolism, Trimethoprim resistance

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase type 7 (EC:1.5.1.3)
Alternative name(s):
Dihydrofolate reductase type VII
Gene namesi
Name:dhfrVII
Encoded oniPlasmid pLMO271 Publication
Plasmid IncP-beta R7511 Publication
Plasmid pDGO1001 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 157157Dihydrofolate reductase type 7
PRO_0000186424Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP27422.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 156155DHFR
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

OMAiPRVEYAR.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27422-1 [UniParc]FASTAAdd to Basket

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MKISLISATS ENGVIGNGPD IPWSAKGEQL LFKALTYNQW LLVGRKTFDS    50
MGVLPNRKYA VVSRKGISSS NENVLVFPSI EIALQELSKI TDHLYVSGGG 100
QIYNSLIEKA DIIHLSTVHV EVEGDINFPK IPENFNLVFE QFFLSNINYT 150
YQIWKKG 157
Length:157
Mass (Da):17,594
Last modified:August 1, 1992 - v1
Checksum:iA7791CC9C7E029DE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58425 Genomic DNA. Translation: CAA41326.1.
U31119 Genomic DNA. Translation: AAB18756.1.
PIRiA47087.
RefSeqiYP_006953993.1. NC_019091.1.

Genome annotation databases

GeneIDi13909542.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58425 Genomic DNA. Translation: CAA41326.1 .
U31119 Genomic DNA. Translation: AAB18756.1 .
PIRi A47087.
RefSeqi YP_006953993.1. NC_019091.1.

3D structure databases

ProteinModelPortali P27422.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13909542.

Phylogenomic databases

OMAi PRVEYAR.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .

Family and domain databases

Gene3Di 3.40.430.10. 1 hit.
InterProi IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
[Graphical view ]
PRINTSi PR00070. DHFR.
SUPFAMi SSF53597. SSF53597. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of transposon Tn5086, carrying the site-specifically inserted gene dhfrVII mediating trimethoprim resistance."
    Sundstroem L., Swedberg G., Skoeld O.
    J. Bacteriol. 175:1796-1805(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Transposon: Tn5086.
    Plasmid: pLMO27
  2. "Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements."
    Raadstroem P., Skoeld O., Swedberg G., Flensburg J., Roy P.H., Sundstroem L.
    J. Bacteriol. 176:3257-3268(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Transposon: Tn5090.
    Plasmid: IncP-beta R751
  3. "Plasmid pDGO100 contains a second integron with the trimethoprim resistance gene dfrA7 as the inserted cassette."
    Burnside J.M., Groot Obbink D.J.
    Plasmid 35:67-70(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: VA292.
    Plasmid: pDGO100

Entry informationi

Entry nameiDYR7_ECOLX
AccessioniPrimary (citable) accession number: P27422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 14, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid, Transposable element

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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