Reviewed,
UniProtKB/Swiss-Prot P27422 (DYR7_ECOLX)
Last modified
February 9, 2010.
Version 61.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Dihydrofolate reductase type 7 EC=1.5.1.3 Alternative name(s): Dihydrofolate reductase type VII | ||
| Gene names |
| ||
| Encoded on | Plasmid pLMO27 Ref.1 Plasmid IncP-beta R751 Ref.2 Plasmid pDGO100 Ref.3 | ||
| Organism | Escherichia coli | ||
| Taxonomic identifier | 562 [NCBI] | ||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 157 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity. |
| Catalytic activity | 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH. |
| Pathway | |
| Subunit structure | Homodimer By similarity. |
| Sequence similarities | Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Antibiotic resistance Methotrexate resistance One-carbon metabolism Trimethoprim resistance |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Plasmid Transposable element |
| Gene Ontology (GO) | |
| Biological process | glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic processInferred from electronic annotation. Source: InterPro one-carbon metabolic processInferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW response to antibioticInferred from electronic annotation. Source: UniProtKB-KW response to methotrexateInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | NADP or NADPH binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| [1] | "Characterization of transposon Tn5086, carrying the site-specifically inserted gene dhfrVII mediating trimethoprim resistance." Sundstroem L., Swedberg G., Skoeld O. J. Bacteriol. 175:1796-1805(1993) [PubMed: 8383666] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Transposon: Tn5086. |
| [2] | "Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements." Raadstroem P., Skoeld O., Swedberg G., Flensburg J., Roy P.H., Sundstroem L. J. Bacteriol. 176:3257-3268(1994) [PubMed: 8195081] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Transposon: Tn5090. |
| [3] | "Plasmid pDGO100 contains a second integron with the trimethoprim resistance gene dfrA7 as the inserted cassette." Burnside J.M., Groot Obbink D.J. Plasmid 35:67-70(1996) [PubMed: 8693029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: VA292. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X58425 Genomic DNA. Translation: CAA41326.1. U31119 Genomic DNA. Translation: AAB18756.1. |
| PIR | A47087. |
3D structure databases | |
| SMR | P27422. Positions 1-156. |
| ModBase | Search... |
Enzyme and pathway databases | |
| BRENDA | 1.5.1.3. 246. |
Family and domain databases | |
| InterPro | IPR012259. DHFR. IPR017925. Dihydrofolate_reductase_CS. IPR001796. Dihydrofolate_reductase_dom. [Graphical view] |
| PANTHER | PTHR11549:SF1. DHFR. 1 hit. |
| Pfam | PF00186. DHFR_1. 1 hit. [Graphical view] |
| PRINTS | PR00070. DHFR. |
| PROSITE | PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DYR7_ECOLX | ||||||||
| Accession | Primary (citable) accession number: P27422 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


