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P27422

- DYR7_ECOLX

UniProt

P27422 - DYR7_ECOLX

Protein

Dihydrofolate reductase type 7

Gene

dhfrVII

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

    Pathwayi

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. glycine biosynthetic process Source: InterPro
    2. nucleotide biosynthetic process Source: InterPro
    3. one-carbon metabolic process Source: UniProtKB-KW
    4. response to antibiotic Source: UniProtKB-KW
    5. response to methotrexate Source: UniProtKB-KW
    6. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Antibiotic resistance, Methotrexate resistance, One-carbon metabolism, Trimethoprim resistance

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrofolate reductase type 7 (EC:1.5.1.3)
    Alternative name(s):
    Dihydrofolate reductase type VII
    Gene namesi
    Name:dhfrVII
    Encoded oniPlasmid pLMO271 Publication
    Plasmid IncP-beta R7511 Publication
    Plasmid pDGO1001 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 157157Dihydrofolate reductase type 7PRO_0000186424Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP27422.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 156155DHFRPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the dihydrofolate reductase family.Curated
    Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    OMAiPRVEYAR.

    Family and domain databases

    Gene3Di3.40.430.10. 1 hit.
    InterProiIPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    [Graphical view]
    PRINTSiPR00070. DHFR.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27422-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKISLISATS ENGVIGNGPD IPWSAKGEQL LFKALTYNQW LLVGRKTFDS    50
    MGVLPNRKYA VVSRKGISSS NENVLVFPSI EIALQELSKI TDHLYVSGGG 100
    QIYNSLIEKA DIIHLSTVHV EVEGDINFPK IPENFNLVFE QFFLSNINYT 150
    YQIWKKG 157
    Length:157
    Mass (Da):17,594
    Last modified:August 1, 1992 - v1
    Checksum:iA7791CC9C7E029DE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58425 Genomic DNA. Translation: CAA41326.1.
    U31119 Genomic DNA. Translation: AAB18756.1.
    PIRiA47087.
    RefSeqiYP_006953993.1. NC_019091.1.

    Genome annotation databases

    GeneIDi13909542.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58425 Genomic DNA. Translation: CAA41326.1 .
    U31119 Genomic DNA. Translation: AAB18756.1 .
    PIRi A47087.
    RefSeqi YP_006953993.1. NC_019091.1.

    3D structure databases

    ProteinModelPortali P27422.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13909542.

    Phylogenomic databases

    OMAi PRVEYAR.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .

    Family and domain databases

    Gene3Di 3.40.430.10. 1 hit.
    InterProi IPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00070. DHFR.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of transposon Tn5086, carrying the site-specifically inserted gene dhfrVII mediating trimethoprim resistance."
      Sundstroem L., Swedberg G., Skoeld O.
      J. Bacteriol. 175:1796-1805(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Transposon: Tn5086.
      Plasmid: pLMO27
    2. "Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements."
      Raadstroem P., Skoeld O., Swedberg G., Flensburg J., Roy P.H., Sundstroem L.
      J. Bacteriol. 176:3257-3268(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Transposon: Tn5090.
      Plasmid: IncP-beta R751
    3. "Plasmid pDGO100 contains a second integron with the trimethoprim resistance gene dfrA7 as the inserted cassette."
      Burnside J.M., Groot Obbink D.J.
      Plasmid 35:67-70(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: VA292.
      Plasmid: pDGO100

    Entry informationi

    Entry nameiDYR7_ECOLX
    AccessioniPrimary (citable) accession number: P27422
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid, Transposable element

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3