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Protein

Viral dihydrofolate reductase

Gene

DHFR

Organism
Saimiriine herpesvirus 2 (strain 484-77) (SaHV-2) (Herpesvirus saimiri)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Viral dihydrofolate reductase (DHFR)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei70SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 22NADPBy similarity7
Nucleotide bindingi54 – 56NADPBy similarity3
Nucleotide bindingi76 – 78NADPBy similarity3
Nucleotide bindingi116 – 123NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Viral dihydrofolate reductase (EC:1.5.1.3)
Short name:
vDHFR
Gene namesi
Name:DHFR
Synonyms:2
OrganismiSaimiriine herpesvirus 2 (strain 484-77) (SaHV-2) (Herpesvirus saimiri)
Taxonomic identifieri10382 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863791 – 186Viral dihydrofolate reductaseAdd BLAST186

Structurei

3D structure databases

ProteinModelPortaliP27421
SMRiP27421
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 184DHFRPROSITE-ProRule annotationAdd BLAST181

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni31 – 36Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P27421-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLLLNCIVA VDQNMGIGKN GYLPWPLLTN DFKYFQRMTT SSVKNKQNLV
60 70 80 90 100
IMGKNTWFSI PEKNRPLKDR INLVLSKKLK EIPHGAHFLA RSLNDALKLI
110 120 130 140 150
EQPEFVNKVD MVWIIGGSSV YKDAMNYSSH LKLFVTRIMQ SFETDTFFPE
160 170 180
IDLKKYKPLI EYPGVPSNTQ EEKGIRYKFE VYEKNY
Length:186
Mass (Da):21,674
Last modified:August 1, 1992 - v1
Checksum:i8249CD3C7ABE1BA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58774 Genomic DNA Translation: CAA41575.1

Similar proteinsi

Entry informationi

Entry nameiDYR_SHV24
AccessioniPrimary (citable) accession number: P27421
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: March 28, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health