Reviewed, UniProtKB/Swiss-Prot P27421 (DYR_SHV24)

Names and origin

Protein names

Recommended name:
    Viral dihydrofolate reductase
      Short name=vDHFR
    EC=1.5.1.3

Gene names

Name:	DHFR
Synonyms:	2

Organism

Saimiriine herpesvirus 2 (strain 484-77) (SaHV-2) (Herpesvirus saimiri)

Taxonomic identifier

10382 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Gammaherpesvirinae › Rhadinovirus

Virus host

Saimiri sciureus (Common squirrel monkey) [TaxID: 9521]

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	Viral dihydrofolate reductase		PRO_0000186379
Regions
	Domain	4 – 184	181	DHFR
	Nucleotide binding	16 – 22	7	NADP By similarity
	Nucleotide binding	54 – 56	3	NADP By similarity
	Nucleotide binding	76 – 78	3	NADP By similarity
	Nucleotide binding	116 – 123	8	NADP By similarity
	Region	31 – 36	6	Substrate binding By similarity
Sites
	Binding site	10	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	70	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27421-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 8249CD3C7ABE1BA2	FASTA	186	21,674
	10 20 30 40 50 60 MVLLLNCIVA VDQNMGIGKN GYLPWPLLTN DFKYFQRMTT SSVKNKQNLV IMGKNTWFSI 70 80 90 100 110 120 PEKNRPLKDR INLVLSKKLK EIPHGAHFLA RSLNDALKLI EQPEFVNKVD MVWIIGGSSV 130 140 150 160 170 180 YKDAMNYSSH LKLFVTRIMQ SFETDTFFPE IDLKKYKPLI EYPGVPSNTQ EEKGIRYKFE VYEKNY

References

[1]	"Small RNA expression from the oncogenic region of a highly oncogenic strain of herpesvirus saimiri." Geck P., Whitaker S.A., Medveczky M.M., Last T.J., Medveczky P.G. Virus Genes 8:25-34(1994) [PubMed: 8209420] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58774 Genomic DNA. Translation: CAA41575.1.
3D structure databases
SMR	P27421. Positions 5-185.
ModBase	Search...
Family and domain databases
InterPro	IPR012259. DHFR. IPR017925. Dihydrofolate_reductase_CS. IPR001796. Dihydrofolate_reductase_dom. [Graphical view]
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_SHV24

Accession

Primary (citable) accession number: P27421

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)