P27411 (POLG_RHDV3) Reviewed, UniProtKB/Swiss-Prot
Last modified April 16, 2014. Version 83. History...
Names and origin
|Protein names||Recommended name:|
Cleaved into the following 11 chains:
|Organism||Rabbit hemorrhagic disease virus (strain V-351) (Ra/LV/RHDV/V351/1991/CK) (RHDV-V351) [Complete proteome]|
|Taxonomic identifier||11977 [NCBI]|
|Taxonomic lineage||Viruses › ssRNA positive-strand viruses, no DNA stage › Caliciviridae › Lagovirus ›|
|Virus host||Oryctolagus cuniculus (Rabbit) [TaxID: 9986]|
|Sequence length||2344 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Inferred from homology|
General annotation (Comments)
NTPase presumably plays a role in replication By similarity.
Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation By similarity.
3C-like protease processes the polyprotein: 3CLpro-RdRp (p72) is first released by autocleavage, then all other proteins are cleaved By similarity.
RNA-directed RNA polymerase replicates genomic and antigenomic RNA by recognizing replications specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs By similarity.
Capsid protein VP60 self assembles to form an icosahedral capsid with a T=3 symmetry, about 35 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate VP2 proteins and genomic or subgenomic RNA. Attaches virion to target cells by binding histo-blood group antigens, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm By similarity.
NTP + H2O = NDP + phosphate.
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Binds to histo-blood group antigens at surface of target cells By similarity.
Specific enzymatic cleavages by its own cysteine protease yield mature proteins. The protease cleaves itself from the nascent polyprotein autocatalytically. Precursor p41 can be cleaved by viral 3CLpro into protein p19 and VPg, or cleaved by host protease into protein p23/2 and protein p18 By similarity.
VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.
Two differents RNAs lead the expression of the capsid protein. One arises from the cleavage of the polyprotein translated from the genomic RNA and the other from the translation of a subgenomic RNA derived from the (-)RNA template. Capsid protein expressed from the subgenomic mRNA is produced in much larger amounts than the cleaved one By similarity.
Contains 1 peptidase C24 domain.
Contains 1 RdRp catalytic domain.
Contains 1 SF3 helicase domain.
|This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]|
|Isoform Genome polyprotein (identifier: P27411-1) |
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
|Note: Produced from the genomic RNA.|
|Isoform Subgenomic capsid protein VP60 (identifier: P27411-2) |
Also known as: VP1;
The sequence of this isoform differs from the canonical sequence as follows:
|Note: Produced from the subgenomic RNA.|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 2344||2344||Genome polyprotein||PRO_0000342011|
|Chain||1 – 143||143||Protein p16 By similarity||PRO_0000036977|
|Chain||144 – 339||196||Protein p23 By similarity||PRO_0000036978|
|Chain||340 – 718||379||NTPase By similarity||PRO_0000036979|
|Chain||712 – 1108||397||Precursor p41 By similarity||PRO_0000342012|
|Chain||712 – 936||225||Protein p23/2 By similarity||PRO_0000342013|
|Chain||719 – 993||275||Protein p29 By similarity||PRO_0000036980|
|Chain||937 – 1108||172||Protein p18||PRO_0000342014|
|Chain||994 – 1108||115||Viral genome-linked protein By similarity||PRO_0000036981|
|Chain||1109 – 1251||143||3C-like protease By similarity||PRO_0000036982|
|Chain||1252 – 1767||516||RNA-directed RNA polymerase By similarity||PRO_0000036983|
|Chain||1768 – 2344||577||Capsid protein VP60 By similarity||PRO_0000036985|
|Domain||492 – 653||162||SF3 helicase|
|Domain||1120 – 1218||99||Peptidase C24|
|Domain||1495 – 1619||125||RdRp catalytic|
|Nucleotide binding||522 – 529||8||ATP Potential|
|Active site||1135||1||For 3CLpro activity By similarity|
|Active site||1159||1||For 3CLpro activity By similarity|
|Active site||1212||1||For 3CLpro activity Potential|
|Site||143 – 144||2||Cleavage; by 3CLpro By similarity|
|Site||339 – 340||2||Cleavage; by 3CLpro By similarity|
|Site||718 – 719||2||Cleavage; by 3CLpro By similarity|
|Site||936 – 937||2||Cleavage; by host By similarity|
|Site||993 – 994||2||Cleavage; by 3CLpro By similarity|
|Site||1108 – 1109||2||Cleavage; by 3CLpro By similarity|
|Site||1251 – 1252||2||Cleavage; by 3CLpro By similarity|
|Site||1767 – 1768||2||Cleavage; by 3CLpro By similarity|
Amino acid modifications
|Modified residue||1014||1||O-(5'-phospho-RNA)-tyrosine By similarity|
|Disulfide bond||1584 ↔ 1591||By similarity|
|Alternative sequence||1 – 1765||1765||Missing in isoform Subgenomic capsid protein VP60.||VSP_034383|
|Sequence conflict||2245||1||A → V in CAA77633. Ref.2|
|||"The complete nucleotide sequence of rabbit haemorrhagic disease virus (Czech strain V351): use of the polymerase chain reaction to detect replication in Australian vertebrates and analysis of viral population sequence variation."|
Gould A.R., Kattenbelt J.A., Lenghaus C., Morrissy C., Chamberlain T., Collins B.J., Westbury H.A.
Virus Res. 47:7-17(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
|||"Genomic 3' terminal sequence comparison of three isolates of rabbit haemorrhagic disease virus."|
Milton I.D., Vlasak R., Nowotny N., Rodak L., Carter M.J.
FEMS Microbiol. Lett. 72:37-42(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 2111-2344.
|U54983 Genomic RNA. Translation: AAB02225.1.|
Z11535 Genomic RNA. Translation: CAA77633.1.
3D structure databases
|SMR||P27411. Positions 1256-1752. |
Protocols and materials databases
Family and domain databases
|Gene3D||18.104.22.1680. 2 hits. |
|InterPro||IPR003593. AAA+_ATPase. |
|Pfam||PF00915. Calici_coat. 1 hit. |
PF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
|PRINTS||PR00916. 2CENDOPTASE. |
|SMART||SM00382. AAA. 1 hit. |
|SUPFAM||SSF50494. SSF50494. 1 hit. |
SSF52540. SSF52540. 1 hit.
|PROSITE||PS50507. RDRP_SSRNA_POS. 1 hit. |
PS51218. SF3_HELICASE_2. 1 hit.
|Accession||Primary (citable) accession number: P27411|
Secondary accession number(s): Q86114
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|