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P27407

- POLG_FCVC6

UniProt

P27407 - POLG_FCVC6

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Protein

Genome polyprotein

Gene

ORF1

Organism
Feline calicivirus (strain CFI/68 FIV) (FCV)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity By similarity.By similarity
Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation By similarity.By similarity
Protease-polymerase p76 processes the polyprotein: Pro-Pol is first released by autocleavage, then all other proteins are cleaved. Cleaves host translation initiation factor eIF4G1, eIF4G2 and PABP1 thereby inducing a shutdown of host protein synthesis. This shutdown may not prevent viral mRNA from being translated since viral Vpg replaces the cap. May cleave host polyadenylate-binding protein thereby inhibiting cellular translation. It is also an RNA-directed RNA polymerase which replicates genomic and antigenomic viral RNA by recognizing specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs By similarity.By similarity

Catalytic activityi

NTP + H2O = NDP + phosphate.
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei46 – 472Cleavage; by Pro-PolBy similarity
Sitei331 – 3322Cleavage; by Pro-PolBy similarity
Sitei684 – 6852Cleavage; by Pro-PolBy similarity
Sitei959 – 9602Cleavage; by Pro-PolBy similarity
Sitei1070 – 10712Cleavage; by Pro-PolBy similarity
Active sitei1109 – 11091For protease activityBy similarity
Active sitei1130 – 11301For protease activityBy similarity
Active sitei1192 – 11921For protease activityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi484 – 4918ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. RNA binding Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. RNA helicase activity Source: InterPro

GO - Biological processi

  1. RNA-protein covalent cross-linking Source: UniProtKB-KW
  2. suppression by virus of host gene expression Source: UniProtKB-KW
  3. transcription, DNA-templated Source: InterPro
  4. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 6 chains:
Alternative name(s):
p39
Alternative name(s):
VPg
p13
Protease-polymerase p76 (EC:2.7.7.48, EC:3.4.22.66)
Short name:
Pro-Pol
Gene namesi
ORF Names:ORF1
OrganismiFeline calicivirus (strain CFI/68 FIV) (FCV)
Taxonomic identifieri11979 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeVesivirus
Virus hostiFelidae (cat family) [TaxID: 9681]
ProteomesiUP000008667: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17621762Genome polyproteinPRO_0000341990Add
BLAST
Chaini1 – 4646Protein p5.6By similarityPRO_0000036892Add
BLAST
Chaini47 – 331285Protein p32By similarityPRO_0000036893Add
BLAST
Chaini332 – 684353NTPaseBy similarityPRO_0000036894Add
BLAST
Chaini685 – 959275Protein p30By similarityPRO_0000036895Add
BLAST
Chaini960 – 1070111Viral genome-linked proteinBy similarityPRO_0000036896Add
BLAST
Chaini1071 – 1762692Protease-polymerase p76By similarityPRO_0000036897Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei983 – 9831O-(5'-phospho-RNA)-tyrosineBy similarity

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. Pro-Pol is first autocatalytically cleaved, then processes the whole polyprotein By similarity.By similarity
VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.By similarity

Keywords - PTMi

Covalent protein-RNA linkage, Phosphoprotein

Interactioni

Subunit structurei

Protein p32: homodimer, interacts with NTPase, protein p30 and Pro-Pol. Viral genome-linked protein interacts with capsid protein and Pro-Pol. Protease-polymerase p76: Homooligomers, interacts with Vpg, protein p32 and may interact with capsid protein By similarity.By similarity

Structurei

3D structure databases

ProteinModelPortaliP27407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini458 – 614157SF3 helicasePROSITE-ProRule annotationAdd
BLAST
Domaini1094 – 1198105Peptidase C24Add
BLAST
Domaini1477 – 1602126RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Domaini

Protease-polymerase is composed of two domains displaying two different catalytic activity. These activities may act independently By similarity.By similarity

Sequence similaritiesi

Contains 1 peptidase C24 domain.Curated
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiIPR003593. AAA+_ATPase.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 4 hits.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27407-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSQTLSFVLK THNVRKDFVR SVKLTLARRR DLQYFYNKLS RSMRAEACPS
60 70 80 90 100
CASYDVCPNC TSSDIPDDGS STELIPSWEE VTKTSTYSLL LSEDTSDELC
110 120 130 140 150
PDDLANVAAH IRKAISTQSH PANSDMCKEQ LTSLLVMAEA MLPQRSRASI
160 170 180 190 200
PLHQQHQAAR LEWREKFFSK PLDFLLERIG VSKDILQITA IWKIILEKAC
210 220 230 240 250
YCKSYGEQWF TTAKQKLREM RSYESNTLKP LIGAFIDGLR FMTVDNPYPM
260 270 280 290 300
GFLPKLIGLI KPLNLAMIID NHENTLSGWV ITLTAIMELY NITECTIDLM
310 320 330 340 350
TSLITAFYDK IGKATKFYSH VKALFTGFRT EDVSNSFWYM AAAILCYLVT
360 370 380 390 400
GLIPNNGRFL KIKCLLVRAT TLVSGIIATQ KLAAMFATWN SESIVNELSA
410 420 430 440 450
RTVAISELNN PTTTSDTESV ERLLELAKIL HEEIKVHTLN PIMQSYNLIL
460 470 480 490 500
RNLMSTLDGV ITCCNKRKAI ARKRQVPVCY ILTGPPGCGK TTAAQALAKK
510 520 530 540 550
LSDQEPSVIN LDVDHHDTYT GNEVCIIDEF DSSDKVDYAN FVIGMVNSAP
560 570 580 590 600
MVLNCDMLEN KGKLFTSKYI IMTSNSETPV KPSSKRAGAF YRRVTYHDVA
610 620 630 640 650
TLVESHKRAR PGTAVPRSCY KKNFSHLSLA KRGAECWCKE YVLDPKGLQH
660 670 680 690 700
QSTKAPPPTF LNIDSLAQTM KQDFALKNMA FEAEVGCSEH RYGFVCQQSE
710 720 730 740 750
VETVRRLLNA IRMRLNATFT VCVGLEASNS VGCTAHVLTP DEPFNGKRFV
760 770 780 790 800
VSRCNEASLS ALEGNCVQTA LGVCMSNKDL THLCHFIKGK IVNDSVRLDE
810 820 830 840 850
LPANQHVVTV NSVFDLAWAL RRHSTLTGQF QAIRAAYDVL HVPDKVPAML
860 870 880 890 900
RHWMDETSFS DEHVVTQFIT PGGVVILESC GGARIWALGN NVIRAGGVTA
910 920 930 940 950
IPTGGCVRLM GLSAQTMPWS EILSELFSLL GKIWSSVKVS TLILTALSMY
960 970 980 990 1000
ASRFRPKTEA KGKTKSKIGP YRGRGVALTD DEYDEWKEHN AARKLDLSVE
1010 1020 1030 1040 1050
DFLMLRHRAA LGADDTDAVK FRSWWNSRSR LADDFEDVTV IGKGGVKHEK
1060 1070 1080 1090 1100
IRTNTLRAVD RGYDVSFAEE SGPGAKFHKN AIGSVTDVCG EHKGYCVHMG
1110 1120 1130 1140 1150
HGVYASVAHV VKGDSFFLGE RIFDLKTNGE FCCFRSTKIL PSAAPFFSGR
1160 1170 1180 1190 1200
PTRDPWGSPV ATDWKPKPYS TTSGKIVGCF ATTSTETHPG DCGLPYIDDN
1210 1220 1230 1240 1250
GRVTGLHTGS GGPKTPSAKL VVPYVHIDMK TKSVTAQKYD VTKPDISYKG
1260 1270 1280 1290 1300
LVCKQLDEIR IIPKGTRLHV SPAHLEDFEE CSHQPASLGS GDPRCPKSLT
1310 1320 1330 1340 1350
AIVVDSLKPY CVVVNGPPHD ILHRVQKMLI DHLSGFVPMN ISSDTSMLSA
1360 1370 1380 1390 1400
FHKLNHDTSC GPYLGGRKKD HMVNGEPDKA LLDLLSSKWK LATQGIALPH
1410 1420 1430 1440 1450
EYTIGLKDEL RPIEKVQEGK RRMIWGCDVG VATVCAAAFK GVSDAITANH
1460 1470 1480 1490 1500
QYGPIQVGIN MDSPSVEALF QRIKSARKVF AVDYSKWDST QSPRVSAASI
1510 1520 1530 1540 1550
DILRYFSDRT PIVDSATNTL KSPPIAVFNG VAVKVSSGLP SGMPLTSVIN
1560 1570 1580 1590 1600
SLNHCLYVGC AILQSLEARN VPVTWNLFST FDMMTYGDDG VYMFPTMYAS
1610 1620 1630 1640 1650
ISDQIFANLS AYGLKPTRVD KSVGSIEPID PNSVVFLKRT ITRTPQGIRG
1660 1670 1680 1690 1700
LLDRSSILRQ FYYIKGENTD NWKEPPKTID PMSRGQQLWN ACLYASQHGI
1710 1720 1730 1740 1750
DFYNKVYKLA EKAVEYEGLH LEPPSYSTAL EHYNSQFNGV EARTDQIDTS
1760
GMAALHCDVF EV
Length:1,762
Mass (Da):195,339
Last modified:May 30, 2000 - v3
Checksum:i61C010202984AF40
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U13992 Genomic RNA. Translation: AAC13992.1.
M32296 Genomic RNA. Translation: AAA42927.1.
PIRiA43488.
T09245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U13992 Genomic RNA. Translation: AAC13992.1 .
M32296 Genomic RNA. Translation: AAA42927.1 .
PIRi A43488.
T09245.

3D structure databases

ProteinModelPortali P27407.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.300. 4 hits.
InterProi IPR003593. AAA+_ATPase.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 4 hits.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Neill J.D.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Nucleotide sequence of a region of the feline calicivirus genome which encodes picornavirus-like RNA-dependent RNA polymerase, cysteine protease and 2C polypeptides."
    Neill J.D.
    Virus Res. 17:145-160(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 479-1762.

Entry informationi

Entry nameiPOLG_FCVC6
AccessioniPrimary (citable) accession number: P27407
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: May 30, 2000
Last modified: October 1, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3