P27406 (CAPSD_FCVF9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Capsid protein

Alternative name(s):
Coat protein
Short name=CP
VP1

Cleaved into the following chain:

1. Protein 40k

Gene names

Name:	CPP76
ORF Names:	ORF2

Organism

Feline calicivirus (strain F9) (FCV) [Complete proteome]

Taxonomic identifier

11981 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Caliciviridae › Vesivirus › Feline calicivirus

Virus host

Felidae (cat family) [TaxID: 9681]

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells by binding to feline junctional adhesion molecule A (F11R) and/or to alpha-2,6-linked sialic acid. Once attached, the virion is endocytosed. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm.
Subunit structure	Homodimerizes, then multimerizes. May bind to VP3 and Vpg proteins. Binds to alpha-2,6-linked sialic acid at surface of target cells. Interacts with host F11R. Ref.6 Ref.7
Subcellular location	Virion. Host cytoplasm.
Post-translational modification	Cleaved by virus calcivirin to produce mature capsid protein. Cleaved by host caspase-2 and caspase-6 to generate protein p40.
Sequence similarities	Belongs to the caliciviridae capsid protein family.

Ontologies

Keywords
Cellular component	Host cytoplasm Virion
Molecular function	Capsid protein T=3 icosahedral capsid protein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular_component	T=3 icosahedral viral capsid Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 124	124			PRO_0000036878
	Chain	125 – 671	547	Capsid protein		PRO_0000036879
	Chain	?465 – 671	207	Protein 40k		PRO_0000341981
Sites
	Site	124 – 125	2	Cleavage; by calicivirin By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27406 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 33BEE86D8370D5E5	FASTA	671	73,441
	10 20 30 40 50 60 MCSTCANVLK YYDWDPHFKL VINPNNFLSV GFCSNPLMCC YPELLPEFGT VWDCDRSPLE 70 80 90 100 110 120 IYLESILGDD EWASTFDAVD PVVPPMHWGA AGKIFQPHPG VLMHHLIGKV AAGWDPDLPL 130 140 150 160 170 180 IRLEADDGSI TAPEQGTMVG GVIAEPSAQM STAADMATGK SVDSEWEAFF SFHTSVNWST 190 200 210 220 230 240 SETQGKILFK QSLGPLLNPY LEHLAKLYVA WSGSIEVRFS ISGSGVFGGK LAAIVVPPGV 250 260 270 280 290 300 DPVQSTSMLQ YPHVLFDARQ VEPVIFCLPD LRSTLYHLMS DTDTTSLVIM VYNDLINPYA 310 320 330 340 350 360 NDANSSGCIV TVETKPGPDF KFHLLKPPGS MLTHGSIPSD LIPKTSSLWI GNRYWSDITD 370 380 390 400 410 420 FVIRPFVFQA NRHFDFNQET AGWSTPRFRP ISVTITEQNG AKLGIGVATD YIVPGIPDGW 430 440 450 460 470 480 PDTTIPGELI PAGDYAITNG TGNDITTATG YDTADIIKNN TNFRGMYICG SLQRAWGDKK 490 500 510 520 530 540 ISNTAFITTA TLDGDNNNKI NPCNTIDQSK IVVFQDNHVG KKAQTSDDTL ALLGYTGIGE 550 560 570 580 590 600 QAIGSDRDRV VRISTLPETG ARGGNHPIFY KNSIKLGYVI RSIDVFNSQI LHTSRQLSLN 610 620 630 640 650 660 HYLLPPDSFA VYRIIDSNGS WFDIGIDSDG FSFVGVSGFG KLEFPLSASY MGIQLAKIRL 670 ASNIRSPMTK L

References

[1]	"The complete nucleotide sequence of a feline calicivirus." Carter M.J., Milton I.D., Meanger J., Bennett M., Gaskell R.M., Turner P.C. Virology 190:443-448(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Cloning and sequence determination of the feline calicivirus strain F9." Meanger J., Carter M.J., Gaskell R.M., Turner P.C. Biochem. Soc. Trans. 20:26S-26S(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Identification and sequence determination of the capsid protein gene of feline calicivirus." Carter M.J., Milton I.D., Turner P.C., Meanger J., Bennett M., Gaskell R.M. Arch. Virol. 122:223-235(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], PROTEIN SEQUENCE OF 125-131, PROTEOLYTIC PROCESSING.
[4]	"Caspase-mediated cleavage of the feline calicivirus capsid protein." Al-Molawi N., Beardmore V.A., Carter M.J., Kass G.E., Roberts L.O. J. Gen. Virol. 84:1237-1244(2003) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE BY HOST CASPASE.
[5]	"Entry of feline calicivirus is dependent on clathrin-mediated endocytosis and acidification in endosomes." Stuart A.D., Brown T.D. J. Virol. 80:7500-7509(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VIRAL ENTRY.
[6]	"Identification of regions and residues in feline junctional adhesion molecule required for feline calicivirus binding and infection." Ossiboff R.J., Parker J.S.L. J. Virol. 81:13608-13621(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HOST F11R.
[7]	"Alpha2,6-linked sialic acid acts as a receptor for Feline calicivirus." Stuart A.D., Brown T.D. J. Gen. Virol. 88:177-186(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ALPHA2,6-LINKED SIALIC ACID.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86379 Genomic RNA. Translation: AAA79327.1. Z11536 Genomic RNA. Translation: CAA77636.1.
PIR	VCWWF9. B43382.
3D structure databases
ProteinModelPortal	P27406.
SMR	P27406. Positions 129-665.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004005. Calicivirus_coat. [Graphical view]
Pfam	PF00915. Calici_coat. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPSD_FCVF9

Accession

Primary (citable) accession number: P27406

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program