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Protein

Calpain-D

Gene

sol

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in eye development.1 Publication
Calcium-regulated non-lysosomal thiol-protease.By similarity

Catalytic activityi

Broad endopeptidase specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1079 – 10791By similarity
Active sitei1245 – 12451By similarity
Active sitei1265 – 12651By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 3535RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri135 – 16430RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri643 – 67331RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri704 – 73330RanBP2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri744 – 77431RanBP2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri927 – 95630RanBP2-type 6PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • nervous system development Source: UniProtKB
  • proteolysis Source: UniProtKB
  • response to stimulus Source: UniProtKB-KW
  • visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.22.B35. 1994.

Protein family/group databases

MEROPSiC02.010.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-D (EC:3.4.22.-)
Alternative name(s):
Calcium-activated neutral proteinase D
Short name:
CANP D
Small optic lobes protein
Gene namesi
Name:sol
Synonyms:CalpD
ORF Names:CG1391
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0003464. sol.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mutants cause specific cells to degenerate in the developing optic lobes, resulting in the absence of certain classes of columnar neurons.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15941594Calpain-DPRO_0000207733Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei250 – 2501Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP27398.
PRIDEiP27398.

PTM databases

iPTMnetiP27398.

Expressioni

Developmental stagei

Present throughout development, with expression levels lower in larvae than other life stages.1 Publication

Gene expression databases

BgeeiFBgn0003464.
ExpressionAtlasiP27398. differential.
GenevisibleiP27398. DM.

Interactioni

Protein-protein interaction databases

BioGridi68777. 74 interactions.
IntActiP27398. 9 interactions.
MINTiMINT-281201.
STRINGi7227.FBpp0088620.

Structurei

3D structure databases

ProteinModelPortaliP27398.
SMRiP27398. Positions 1001-1320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1014 – 1321308Calpain catalyticPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi421 – 46040Ser-richAdd
BLAST
Compositional biasi470 – 50940Ala-richAdd
BLAST
Compositional biasi607 – 62923Ala-richAdd
BLAST
Compositional biasi673 – 69927Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 6 RanBP2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 3535RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri135 – 16430RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri643 – 67331RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri704 – 73330RanBP2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri744 – 77431RanBP2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri927 – 95630RanBP2-type 6PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00750000117668.
InParanoidiP27398.
KOiK08582.
OMAiYENECVA.
OrthoDBiEOG091G02OL.
PhylomeDBiP27398.

Family and domain databases

Gene3Di4.10.1060.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF00648. Peptidase_C2. 1 hit.
PF00641. zf-RanBP. 6 hits.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00230. CysPc. 1 hit.
SM00547. ZnF_RBZ. 6 hits.
[Graphical view]
SUPFAMiSSF90209. SSF90209. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS01358. ZF_RANBP2_1. 5 hits.
PS50199. ZF_RANBP2_2. 4 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform BImported (identifier: P27398-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTISSVLQW SCTKCNTINP TESLKCFNCG TVRKVFPQQQ QQQHRSSSIT
60 70 80 90 100
ASWTADDALE QEQAEKGQER DKEKGRAAVA RSEYKHVYKS LLRGCLKRPQ
110 120 130 140 150
RNSQNLPANC VDCEDTRKYI KSSIELYRHF SNPALNRRWV CHACGTDNSS
160 170 180 190 200
VTWHCLICDT VSYLAPIYKD AIAADRGQDL AGSLGNRGEL LAADHSHPHH
210 220 230 240 250
HHHYLHQELE EQHQHQLHSQ HLHKRHLKGR SASGSGSGPG SGSGLRRTQS
260 270 280 290 300
LSTAIDKSAS GRSCHICYAN NQSKDIFNLP QIKPAPQLTG IPPVAACSNS
310 320 330 340 350
RFAIANDTFC RRKQNNNNKN QNHKVVRESG AKRKYNFTIT TLSRSAAKDA
360 370 380 390 400
GHGQMKPLRQ VVNLNLNLQQ EPQQKSPANP QQLQRKTQRE PAAVSMNPTQ
410 420 430 440 450
FTIPRNGVFI AVNEWSEPMA SSSSVSSSSN HHHHHHSNSN SNSSGNSNII
460 470 480 490 500
NNNSSSSSGS NKLYENECVA LAQQQLRAAA AQAAQAAATA VAIASSPSAK
510 520 530 540 550
AMAEPAPTAT MPIYAQVNKQ HKLKKKQQIA SESQTNNNTG SGEIADAVSE
560 570 580 590 600
SLTAGLGTST DGSGEASESE SQVEEHSIYA KVWKGPRKAT ESKIMHDPGS
610 620 630 640 650
SSRLSGAASA AAGTASAGAI AAAVGAAAAS RHDNKTQLGN GSRSKMWICI
660 670 680 690 700
KCSYAYNRLW LQTCEMCEAK AEQQQQQLQL QQQQQQQQQH HHHHLQQQQA
710 720 730 740 750
EAPRDEPWTC KKCTLVNYST AMACVVCGGS KLKSISSIED MTLRKGEFWT
760 770 780 790 800
CSHCTLKNSL HSPVCSACKS HRQPQLSMAM EAVRERPDGQ SYEEQDAAAV
810 820 830 840 850
GGGGGSAHQS GANEVKAPTA LNLPLTSVAL PMPMLQLPTS TAAGLRGSRS
860 870 880 890 900
PSPRMQLLPS LQQQRNSSSS GAIPKRHSTG GSIVPRNISI AGLANYNLQQ
910 920 930 940 950
GQGVGSASVV SASGAGSGAG AVGASTSTKK WQCPACTYDN CAASVVCDIC
960 970 980 990 1000
SSPRGLASAV LGEALGRKSV RVALTPADIR QESKLMENLR QLEETEALTK
1010 1020 1030 1040 1050
WQNIIQYCRD NSELFVDDSF PPAPKSLYYN PASGAGEGNP VVQWRRPHEI
1060 1070 1080 1090 1100
NCDGGAYPPW AVFRTPLPSD ICQGVLGNCW LLSALAVLAE REDLVKEVLV
1110 1120 1130 1140 1150
TKEICGQGAY QVRLCKDGKW TTVLVDDLLP CDKRGHLVYS QAKRKQLWVP
1160 1170 1180 1190 1200
LIEKAVAKIH GCYEALVSGR AIEGLATLTG APCESIPLQA SSLPMPSEDE
1210 1220 1230 1240 1250
LDKDLIWAQL LSSRCVRFLM GASCGGGNMK VDEEEYQQKG LRPRHAYSVL
1260 1270 1280 1290 1300
DVKDIQGHRL LKLRNPWGHY SWRGDWSDDS SLWTDDLRDA LMPHGASEGV
1310 1320 1330 1340 1350
FWISFEDVLN YFDCIDICKV RSGWNEVRLQ GTLQPLCSIS CVLLTVLEPT
1360 1370 1380 1390 1400
EAEFTLFQEG QRNSEKSQRS QLDLCVVIFR TRSPAAPEIG RLVEHSKRQV
1410 1420 1430 1440 1450
RGFVGCHKML ERDIYLLVCL AFNHWHTGIE DPHQYPQCIL AIHSSKRLLV
1460 1470 1480 1490 1500
EQISPSPHLL ADAIISLTLT KGQRHEGREG MTAYYLTKGW AGLVVMVENR
1510 1520 1530 1540 1550
HENKWIHVKC DCQESYNVVS TRGELKTVDS VPPLQRQVII VLTQLEGSGG
1560 1570 1580 1590
FSIAHRLTHR LANSRGLHDW GPPGATHCPP IENVHGLHAP RLIT
Length:1,594
Mass (Da):174,313
Last modified:October 25, 2004 - v2
Checksum:i1CDDE8090B5DA198
GO
Isoform AImported (identifier: P27398-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     394-395: VS → YA
     396-1594: Missing.

Show »
Length:395
Mass (Da):44,202
Checksum:i2740C711A619780A
GO
Isoform EImported (identifier: P27398-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     701-701: Missing.

Show »
Length:1,593
Mass (Da):174,184
Checksum:i2B989757525814BD
GO

Sequence cautioni

The sequence AAS65411 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti384 – 3841Q → H in AAB95431 (PubMed:1714593).Curated
Sequence conflicti623 – 6231A → G in AAB95431 (PubMed:1714593).Curated
Sequence conflicti679 – 6791Q → H in AAB95431 (PubMed:1714593).Curated
Sequence conflicti694 – 6941H → HHHH in AAB95431 (PubMed:1714593).Curated
Sequence conflicti694 – 6941H → HHHH in AAC28409 (PubMed:9144213).Curated
Sequence conflicti836 – 8361Q → R in AAM75061 (PubMed:12537569).Curated
Sequence conflicti915 – 9151A → G in AAC28409 (PubMed:9144213).Curated
Sequence conflicti928 – 9281T → S in AAB95431 (PubMed:1714593).Curated
Sequence conflicti928 – 9281T → S in AAC28409 (PubMed:9144213).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei394 – 3952VS → YA in isoform A. 1 PublicationVSP_011791
Alternative sequencei396 – 15941199Missing in isoform A. 1 PublicationVSP_005246Add
BLAST
Alternative sequencei701 – 7011Missing in isoform E. CuratedVSP_058143

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64084 mRNA. Translation: AAB95431.1.
AF017777 Genomic DNA. Translation: AAC28409.1.
AE014298 Genomic DNA. Translation: AAF50826.4.
AE014298 Genomic DNA. Translation: AAF50827.3.
AE014298 Genomic DNA. Translation: AAS65411.2. Sequence problems.
AY128468 mRNA. Translation: AAM75061.1.
PIRiA41146. BVFFSL.
T08428.
RefSeqiNP_476737.3. NM_057389.6. [P27398-2]
NP_476738.3. NM_057390.6. [P27398-1]
NP_996524.2. NM_206801.3.
UniGeneiDm.2998.

Genome annotation databases

EnsemblMetazoaiFBtr0089678; FBpp0088620; FBgn0003464. [P27398-1]
GeneIDi44014.
KEGGidme:Dmel_CG1391.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64084 mRNA. Translation: AAB95431.1.
AF017777 Genomic DNA. Translation: AAC28409.1.
AE014298 Genomic DNA. Translation: AAF50826.4.
AE014298 Genomic DNA. Translation: AAF50827.3.
AE014298 Genomic DNA. Translation: AAS65411.2. Sequence problems.
AY128468 mRNA. Translation: AAM75061.1.
PIRiA41146. BVFFSL.
T08428.
RefSeqiNP_476737.3. NM_057389.6. [P27398-2]
NP_476738.3. NM_057390.6. [P27398-1]
NP_996524.2. NM_206801.3.
UniGeneiDm.2998.

3D structure databases

ProteinModelPortaliP27398.
SMRiP27398. Positions 1001-1320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68777. 74 interactions.
IntActiP27398. 9 interactions.
MINTiMINT-281201.
STRINGi7227.FBpp0088620.

Protein family/group databases

MEROPSiC02.010.

PTM databases

iPTMnetiP27398.

Proteomic databases

PaxDbiP27398.
PRIDEiP27398.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089678; FBpp0088620; FBgn0003464. [P27398-1]
GeneIDi44014.
KEGGidme:Dmel_CG1391.

Organism-specific databases

CTDi44014.
FlyBaseiFBgn0003464. sol.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00750000117668.
InParanoidiP27398.
KOiK08582.
OMAiYENECVA.
OrthoDBiEOG091G02OL.
PhylomeDBiP27398.

Enzyme and pathway databases

BRENDAi3.4.22.B35. 1994.

Miscellaneous databases

GenomeRNAii44014.
PROiP27398.

Gene expression databases

BgeeiFBgn0003464.
ExpressionAtlasiP27398. differential.
GenevisibleiP27398. DM.

Family and domain databases

Gene3Di4.10.1060.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF00648. Peptidase_C2. 1 hit.
PF00641. zf-RanBP. 6 hits.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00230. CysPc. 1 hit.
SM00547. ZnF_RBZ. 6 hits.
[Graphical view]
SUPFAMiSSF90209. SSF90209. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS01358. ZF_RANBP2_1. 5 hits.
PS50199. ZF_RANBP2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAND_DROME
AccessioniPrimary (citable) accession number: P27398
Secondary accession number(s): O61346
, Q7KU57, Q7KU58, Q8MQP3, Q9VRH3, Q9VRH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 25, 2004
Last modified: September 7, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Although homology to other calpains is high within the protease domain, the lack of calcium-binding sites suggests that this protein is a protease that may not be activated by calcium ions.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.