P27381 (VP09_BPPRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA packaging protein

Alternative name(s):
Protein P9

Gene names

Name:

IX

Organism

Enterobacteria phage PRD1 (Bacteriophage PRD1) [Reference proteome]

Taxonomic identifier

10658 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Tectiviridae › Tectivirus

Virus host

Acinetobacter calcoaceticus [TaxID: 471]
Escherichia coli [TaxID: 562]
Proteus mirabilis [TaxID: 584]
Pseudomonas aeruginosa [TaxID: 287]
Pseudomonas fluorescens [TaxID: 294]
Pseudomonas putida (Arthrobacter siderocapsulatus) [TaxID: 303]
Salmonella typhimurium [TaxID: 90371]
Vibrio cholerae [TaxID: 666]

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA packaging protein. Ref.3
Subcellular location	Virion membrane; Peripheral membrane protein. Note: Localizes at the unique vertex of the capsid, which extends to the internal membrane via two small integral membrane proteins, P20 and P22. Minor capsid protein P6 attaches to the virion via these proteins and is, in turn, necessary for the attachment of DNA packaging protein P9 to the virion.

Ontologies

Keywords
Biological process	Viral genome packaging
Cellular component	Membrane Virion
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed; by host Ref.1
	Chain	2 – 227	226	DNA packaging protein		PRO_0000165351
Regions
	Nucleotide binding	16 – 23	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P27381 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 1125CCF088FC0DEB	FASTA	227	25,787
	10 20 30 40 50 60 MTIRMPNDRQ RILVLGKTGT GKTCAAVWHL SQKDFKRKAW IVLNHKGDDL IDSIEGANHV 70 80 90 100 110 120 DLNFRPKKPG LYIYHPIPDV DDAEVTQLLW DIHAMGDIGV YVDEGYMIPN RDPAFQALLT 130 140 150 160 170 180 QGRSKKIPMI ILSQRPVWLT RFAISESDFF QIFQLGDQRD RQTVQGFVPV DLEKLMQAPV 190 200 210 220 NTVPALKKFH SIYYDVGANN CVIMTPVPTA DAVLARFDLG KKRKQTL

References

[1]	"Genome organization of membrane-containing bacteriophage PRD1." Bamford J.K.H., Haenninen A.-L., Pakula T.M., Ojala P.M., Kalkkinen N., Frilander M., Bamford D.H. Virology 183:658-676(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-7.
[2]	"A snapshot of viral evolution from genome analysis of the tectiviridae family." Saren A.M., Ravantti J.J., Benson S.D., Burnett R.M., Paulin L., Bamford D.H., Bamford J.K.H. J. Mol. Biol. 350:427-440(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"In vitro DNA packaging of PRD1: a common mechanism for internal-membrane viruses." Stromsten N.J., Bamford D.H., Bamford J.K.H. J. Mol. Biol. 348:617-629(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY848689 Genomic DNA. Translation: AAX45927.1.
PIR	WMBPQ9. I40477.
RefSeq	NP_040689.1. NC_001421.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1260942.
Family and domain databases
InterPro	IPR027417. P-loop_NTPase. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VP09_BPPRD

Accession

Primary (citable) accession number: P27381
Secondary accession number(s): Q3T4N5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 53 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program