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Protein

3 beta-hydroxysteroid dehydrogenase type 5

Gene

Hsd3b5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD V can only catalyze the conversion of dihydrotestosterone to 5 alpha-androstanediol in the presence of the cofactor NADPH. Exhibits only 3-ketosteroid reductase activity in the presence of NADPH and does not function as an isomerase.

Catalytic activityi

3-beta-hydroxy-Delta(5)-steroid + NADP+ = 3-oxo-Delta(5)-steroid + NADPH.

Pathwayi: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei155Proton acceptorBy similarity1
Binding sitei159NADBy similarity1

GO - Molecular functioni

  • 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: RGD
  • NAD binding Source: RGD
  • steroid binding Source: RGD
  • steroid dehydrogenase activity Source: RGD

GO - Biological processi

  • progesterone metabolic process Source: RGD
  • regulation of steroid biosynthetic process Source: RGD
  • steroid biosynthetic process Source: RGD

Keywordsi

Molecular functionOxidoreductase
Biological processSteroidogenesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase type 5
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase type V
Short name:
3-beta-HSD V
3-beta-hydroxy-5-ene steroid dehydrogenase
NADPH-dependent 3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.-)
Progesterone reductase
Gene namesi
Name:Hsd3b5
Synonyms:Hsd3b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2838. Hsd3b5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000877891 – 3733 beta-hydroxysteroid dehydrogenase type 5Add BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei350N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP27364.
PRIDEiP27364.

PTM databases

iPTMnetiP27364.
PhosphoSitePlusiP27364.

Expressioni

Tissue specificityi

Expressed predominantly in male liver.

Gene expression databases

BgeeiENSRNOG00000019417.

Interactioni

Protein-protein interaction databases

MINTiMINT-4568006.
STRINGi10116.ENSRNOP00000026290.

Structurei

3D structure databases

ProteinModelPortaliP27364.
SMRiP27364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1430. Eukaryota.
COG0451. LUCA.
HOGENOMiHOG000167989.
HOVERGENiHBG000014.
InParanoidiP27364.
KOiK00070.
PhylomeDBiP27364.
TreeFamiTF343138.

Family and domain databases

InterProiView protein in InterPro
IPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF01073. 3Beta_HSD. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.

Sequencei

Sequence statusi: Complete.

P27364-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGWSCLVTG AGGFLGQRIV QMLVQEKELQ EVRVLYRTFS PKHKEELSKL
60 70 80 90 100
QTKAKVTVLR GDIVDAQFLR RACQGMSVII HTAAALDIAG FLPRQTILDV
110 120 130 140 150
NVKGTQLLLD ACVEASVPAF IYSSSTGVAG PNSYKETILN DREEEHREST
160 170 180 190 200
WSNPYPYSKR MAEKAVLAAN GSILKNGGTF HTCALRLPFI YGEESQIIST
210 220 230 240 250
MVNRALKNNS IIKRHATFSI ANPVYVGNAA WAHILAARGL RDPEKSQSIQ
260 270 280 290 300
GQFYYISDDT PHQSYDDLNY TLSKEWGFCL DSSWSLPLPL LYWLAFLLET
310 320 330 340 350
VSFLLRPFYN YRPPFNRFMV TILNSVFTIS YKKAQRDLGY EPLVSWEEAK
360 370
QKTSEWIGTL VEQHRETLDT KSQ
Length:373
Mass (Da):42,206
Last modified:January 23, 2007 - v3
Checksum:iB96538F2EF056935
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180F → L in AAH92571 (PubMed:15489334).Curated1
Sequence conflicti204R → T in AAH92571 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67465 mRNA. Translation: AAA41352.1.
BC092571 mRNA. Translation: AAH92571.1.
PIRiA37404.
RefSeqiNP_036716.1. NM_012584.1.
UniGeneiRn.34902.

Genome annotation databases

GeneIDi24470.
KEGGirno:24470.
UCSCiRGD:2838. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67465 mRNA. Translation: AAA41352.1.
BC092571 mRNA. Translation: AAH92571.1.
PIRiA37404.
RefSeqiNP_036716.1. NM_012584.1.
UniGeneiRn.34902.

3D structure databases

ProteinModelPortaliP27364.
SMRiP27364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4568006.
STRINGi10116.ENSRNOP00000026290.

PTM databases

iPTMnetiP27364.
PhosphoSitePlusiP27364.

Proteomic databases

PaxDbiP27364.
PRIDEiP27364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24470.
KEGGirno:24470.
UCSCiRGD:2838. rat.

Organism-specific databases

CTDi15496.
RGDi2838. Hsd3b5.

Phylogenomic databases

eggNOGiKOG1430. Eukaryota.
COG0451. LUCA.
HOGENOMiHOG000167989.
HOVERGENiHBG000014.
InParanoidiP27364.
KOiK00070.
PhylomeDBiP27364.
TreeFamiTF343138.

Enzyme and pathway databases

UniPathwayiUPA00062.

Miscellaneous databases

PROiPR:P27364.

Gene expression databases

BgeeiENSRNOG00000019417.

Family and domain databases

InterProiView protein in InterPro
IPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF01073. 3Beta_HSD. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry namei3BHS5_RAT
AccessioniPrimary (citable) accession number: P27364
Secondary accession number(s): Q569C6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: May 10, 2017
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.