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Protein

Thymidine kinase

Gene

TK

Organism
Suid herpesvirus 1 (strain NIA-3) (SuHV-1) (Pseudorabies virus (strain NIA-3))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors.UniRule annotation

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei33 – 331Proton acceptorUniRule annotation
Binding sitei51 – 511SubstrateUniRule annotation
Binding sitei75 – 751SubstrateUniRule annotation
Binding sitei162 – 1621ATPUniRule annotation
Binding sitei168 – 1681SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:TKUniRule annotation
OrganismiSuid herpesvirus 1 (strain NIA-3) (SuHV-1) (Pseudorabies virus (strain NIA-3))
Taxonomic identifieri10349 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Thymidine kinasePRO_0000175082Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP27363.
SMRiP27363. Positions 2-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the herpesviridae thymidine kinase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P27363-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILRIYLDG AYDTGKSTTA RVMALGGALY VPEPMAYWRT LFDTDTVAGI
60 70 80 90 100
YDAQTRKQNG SLSEEDAALV TAHDQAAFAT PYLLLHTRLV PLFGPAVEGP
110 120 130 140 150
PEMTVVFDRH PVAATVCFPL ARFIVGDISA AAFVGLAATL PGEPPGGNLV
160 170 180 190 200
VASLDPDEHL RRLRARARAG EHVDARLLTA LRNVYAMLVN TSRYLSSGRR
210 220 230 240 250
WRDDWGRAPR FDQTTRDCLA LNELCRPRDD PELQDTLFGA YKAPELCDRR
260 270 280 290 300
GRPLEVHAWA MDALVAKLLP LRVSTVDLGP SPRVCAAAVA AQTRGMEVTE
310 320
SAYGDHIRQC VCAFTSEMGV
Length:320
Mass (Da):35,025
Last modified:August 1, 1992 - v1
Checksum:i6833F1FD4D9ED15C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55001 Genomic DNA. Translation: CAA38747.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55001 Genomic DNA. Translation: CAA38747.1.

3D structure databases

ProteinModelPortaliP27363.
SMRiP27363. Positions 2-320.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_SUHVN
AccessioniPrimary (citable) accession number: P27363
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 7, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.