Reviewed, UniProtKB/Swiss-Prot P27355 (MEMG_METTR)

Names and origin

Protein names

Recommended name:
    Methane monooxygenase component A gamma chain
    EC=1.14.13.25
Alternative name(s):
    Methane hydroxylase

Gene names

Name:

mmoZ

Organism

Methylosinus trichosporium

Taxonomic identifier

426 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylocystaceae › Methylosinus

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds.
Catalytic activity	Methane + NAD(P)H + O2 = methanol + NAD(P)+ + H2O.
Subunit structure	M.trichosporium has two forms of methane monooxygenase, a soluble and a membrane-bound type. The soluble type consists of four components (A to D): protein A, comprising three chains, in an alpha-2, beta-2, gamma-2 configuration, is a nonheme iron protein containing an unusual mu-hydroxo bridge structure at its active site and interacts with both oxygen and methane.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	methane metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methane monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 169

168

Methane monooxygenase component A gamma chain

PRO_0000096410

Secondary structure

1

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169

Helix Strand Turn

Details...

Beta strand

7 – 9

3

Helix

11 – 21

11

Helix

26 – 40

15

Turn

49 – 53

5

Helix

54 – 72

19

Helix

75 – 80

6

Helix

88 – 100

13

Helix

105 – 119

15

Turn

120 – 123

4

Helix

126 – 144

19

Turn

145 – 149

5

Helix

153 – 160

8

Beta strand

163 – 167

5

Sequences

	Sequence		Length	Mass (Da)
	P27355-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 460D4D8D234C2229	FASTA	169	19,326
	10 20 30 40 50 60 MAKREPIHDN SIRTEWEAKI AKLTSVDQAT KFIQDFRLAY TSPFRKSYDI DVDYQYIERK 70 80 90 100 110 120 IEEKLSVLKT EKLPVADLIT KATTGEDRAA VEATWIAKIK AAKSKYEADG IHIEFRQLYK 130 140 150 160 PPVLPVNVFL RTDAALGTVL MEIRNTDYYG TPLEGLRKEP GVKVLHLQA

References

[1]	"Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b." Cardy D.L.N., Laidler V., Salmond G.P.C., Murrell J.C. Mol. Microbiol. 5:335-342(1991) [PubMed: 1904125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: OB3b.
[2]	"Complex formation between the protein components of methane monooxygenase from Methylosinus trichosporium OB3b. Identification of sites of component interaction." Fox B.G., Liu Y., Dege J.E., Lipscomb J.D. J. Biol. Chem. 266:540-550(1991) [PubMed: 1845980] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-12.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X55394 Genomic DNA. Translation: CAA39071.1.

PIR

C39049. S15210.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MHY	X-ray	2.00	G	1-169	[»]
1MHZ	X-ray	2.70	G	1-169	[»]

ModBase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MON-3869.

BRENDA

1.14.13.25. 20437.

Family and domain databases

InterPro

IPR004222. Me_mOase_g.
IPR015952. Me_mOase_g_dom1.
IPR015953. Me_mOase_g_dom2.
[Graphical view]

Gene3D

G3DSA:1.20.1280.10. Me_mOase_hydro_g_dom1. 1 hit.
G3DSA:1.20.1280.30. Me_mOase_hydro_g_dom2. 1 hit.

Pfam

PF02964. MeMO_Hyd_G. 1 hit.
[Graphical view]

PIRSF

PIRSF018503. Me_mOase_g. 1 hit.

ProDom

PD022203. MeMO_hydro_gamma. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

MEMG_METTR

Accession

Primary (citable) accession number: P27355

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)