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P27355 (MEMG_METTR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methane monooxygenase component A gamma chain
EC=1.14.13.25
Alternative name(s):
Methane hydroxylase
Gene names
Name:mmoZ
OrganismMethylosinus trichosporium
Taxonomic identifier426 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylocystaceae › Methylosinus

Protein attributes

Sequence length169 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds.

Catalytic activity

Methane + NAD(P)H + O2 = methanol + NAD(P)+ + H2O.

Subunit structure

M.trichosporium has two forms of methane monooxygenase, a soluble and a membrane-bound type. The soluble type consists of four components (A to D): protein A, comprising three chains, in an alpha-2, beta-2, gamma-2 configuration, is a nonheme iron protein containing an unusual mu-hydroxo bridge structure at its active site and interacts with both oxygen and methane.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandNADP
   Molecular functionMonooxygenase
Oxidoreductase
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processmethane metabolic process

Inferred from electronic annotation. Source: InterPro

one-carbon metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionmethane monooxygenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 169168Methane monooxygenase component A gamma chain
PRO_0000096410

Secondary structure

1 ........................ 169
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P27355 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 460D4D8D234C2229

FASTA16919,326
        10         20         30         40         50         60 
MAKREPIHDN SIRTEWEAKI AKLTSVDQAT KFIQDFRLAY TSPFRKSYDI DVDYQYIERK 

        70         80         90        100        110        120 
IEEKLSVLKT EKLPVADLIT KATTGEDRAA VEATWIAKIK AAKSKYEADG IHIEFRQLYK 

       130        140        150        160 
PPVLPVNVFL RTDAALGTVL MEIRNTDYYG TPLEGLRKEP GVKVLHLQA

« Hide

References

[1]"Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b."
Cardy D.L.N., Laidler V., Salmond G.P.C., Murrell J.C.
Mol. Microbiol. 5:335-342(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: OB3b.
[2]"Complex formation between the protein components of methane monooxygenase from Methylosinus trichosporium OB3b. Identification of sites of component interaction."
Fox B.G., Liu Y., Dege J.E., Lipscomb J.D.
J. Biol. Chem. 266:540-550(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-12.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X55394 Genomic DNA. Translation: CAA39071.1.
PIRC39049. S15210.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MHYX-ray2.00G1-169[»]
1MHZX-ray2.70G1-169[»]
ProteinModelPortalP27355.
SMRP27355. Positions 2-168.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-3869.

Family and domain databases

Gene3D1.20.1280.10. 1 hit.
1.20.1280.30. 1 hit.
InterProIPR004222. Me_mOase_g.
IPR015952. Me_mOase_g_dom1.
IPR015953. Me_mOase_g_dom2.
[Graphical view]
PfamPF02964. MeMO_Hyd_G. 1 hit.
[Graphical view]
PIRSFPIRSF018503. Me_mOase_g. 1 hit.
ProDomPD022203. Me_mOase_g. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF47152. Me_mOase_hydro_g. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP27355.

Entry information

Entry nameMEMG_METTR
AccessionPrimary (citable) accession number: P27355
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: April 3, 2013
This is version 69 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents