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P27355

- MEMG_METTR

UniProt

P27355 - MEMG_METTR

Protein

Methane monooxygenase component A gamma chain

Gene

mmoZ

Organism
Methylosinus trichosporium
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds.

    Catalytic activityi

    Methane + NAD(P)H + O2 = methanol + NAD(P)+ + H2O.

    GO - Molecular functioni

    1. methane monooxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. methane metabolic process Source: InterPro
    2. one-carbon metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-3869.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methane monooxygenase component A gamma chain (EC:1.14.13.25)
    Alternative name(s):
    Methane hydroxylase
    Gene namesi
    Name:mmoZ
    OrganismiMethylosinus trichosporium
    Taxonomic identifieri426 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylocystaceaeMethylosinus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 169168Methane monooxygenase component A gamma chainPRO_0000096410Add
    BLAST

    Interactioni

    Subunit structurei

    M.trichosporium has two forms of methane monooxygenase, a soluble and a membrane-bound type. The soluble type consists of four components (A to D): protein A, comprising three chains, in an alpha-2, beta-2, gamma-2 configuration, is a nonheme iron protein containing an unusual mu-hydroxo bridge structure at its active site and interacts with both oxygen and methane.

    Structurei

    Secondary structure

    1
    169
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 93
    Helixi11 – 2111
    Helixi26 – 4015
    Turni49 – 535
    Helixi54 – 7219
    Helixi75 – 806
    Helixi88 – 10013
    Helixi105 – 11915
    Turni120 – 1234
    Helixi126 – 14419
    Turni145 – 1495
    Helixi153 – 1608
    Beta strandi163 – 1675

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MHYX-ray2.00G1-169[»]
    1MHZX-ray2.70G1-169[»]
    ProteinModelPortaliP27355.
    SMRiP27355. Positions 2-168.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP27355.

    Family & Domainsi

    Family and domain databases

    Gene3Di1.20.1280.10. 1 hit.
    1.20.1280.30. 1 hit.
    InterProiIPR004222. Me_mOase_g.
    IPR015952. Me_mOase_g_dom1.
    IPR015953. Me_mOase_g_dom2.
    [Graphical view]
    PfamiPF02964. MeMO_Hyd_G. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018503. Me_mOase_g. 1 hit.
    ProDomiPD022203. Me_mOase_g. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF47152. SSF47152. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27355-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKREPIHDN SIRTEWEAKI AKLTSVDQAT KFIQDFRLAY TSPFRKSYDI    50
    DVDYQYIERK IEEKLSVLKT EKLPVADLIT KATTGEDRAA VEATWIAKIK 100
    AAKSKYEADG IHIEFRQLYK PPVLPVNVFL RTDAALGTVL MEIRNTDYYG 150
    TPLEGLRKEP GVKVLHLQA 169
    Length:169
    Mass (Da):19,326
    Last modified:January 23, 2007 - v3
    Checksum:i460D4D8D234C2229
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55394 Genomic DNA. Translation: CAA39071.1.
    PIRiS15210. C39049.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55394 Genomic DNA. Translation: CAA39071.1 .
    PIRi S15210. C39049.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MHY X-ray 2.00 G 1-169 [» ]
    1MHZ X-ray 2.70 G 1-169 [» ]
    ProteinModelPortali P27355.
    SMRi P27355. Positions 2-168.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-3869.

    Miscellaneous databases

    EvolutionaryTracei P27355.

    Family and domain databases

    Gene3Di 1.20.1280.10. 1 hit.
    1.20.1280.30. 1 hit.
    InterProi IPR004222. Me_mOase_g.
    IPR015952. Me_mOase_g_dom1.
    IPR015953. Me_mOase_g_dom2.
    [Graphical view ]
    Pfami PF02964. MeMO_Hyd_G. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018503. Me_mOase_g. 1 hit.
    ProDomi PD022203. Me_mOase_g. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF47152. SSF47152. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b."
      Cardy D.L.N., Laidler V., Salmond G.P.C., Murrell J.C.
      Mol. Microbiol. 5:335-342(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: OB3b.
    2. "Complex formation between the protein components of methane monooxygenase from Methylosinus trichosporium OB3b. Identification of sites of component interaction."
      Fox B.G., Liu Y., Dege J.E., Lipscomb J.D.
      J. Biol. Chem. 266:540-550(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-12.

    Entry informationi

    Entry nameiMEMG_METTR
    AccessioniPrimary (citable) accession number: P27355
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 71 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3