Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methane monooxygenase component A gamma chain

Gene

mmoZ

Organism
Methylosinus trichosporium
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds.

Catalytic activityi

Methane + NAD(P)H + O2 = methanol + NAD(P)+ + H2O.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3869.

Names & Taxonomyi

Protein namesi
Recommended name:
Methane monooxygenase component A gamma chain (EC:1.14.13.25)
Alternative name(s):
Methane hydroxylase
Gene namesi
Name:mmoZ
OrganismiMethylosinus trichosporium
Taxonomic identifieri426 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylocystaceaeMethylosinus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000964102 – 169Methane monooxygenase component A gamma chainAdd BLAST168

Interactioni

Subunit structurei

M.trichosporium has two forms of methane monooxygenase, a soluble and a membrane-bound type. The soluble type consists of four components (A to D): protein A, comprising three chains, in an alpha-2, beta-2, gamma-2 configuration, is a nonheme iron protein containing an unusual mu-hydroxo bridge structure at its active site and interacts with both oxygen and methane.

Protein-protein interaction databases

STRINGi595536.MettrDRAFT_0738.

Structurei

Secondary structure

1169
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 9Combined sources3
Helixi11 – 21Combined sources11
Helixi26 – 40Combined sources15
Turni49 – 53Combined sources5
Helixi54 – 72Combined sources19
Helixi75 – 80Combined sources6
Helixi88 – 100Combined sources13
Helixi105 – 119Combined sources15
Turni120 – 123Combined sources4
Helixi126 – 144Combined sources19
Turni145 – 149Combined sources5
Helixi153 – 160Combined sources8
Beta strandi163 – 167Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MHYX-ray2.00G1-169[»]
1MHZX-ray2.70G1-169[»]
ProteinModelPortaliP27355.
SMRiP27355.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27355.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105J38. Bacteria.
ENOG4111P1K. LUCA.

Family and domain databases

Gene3Di1.20.1280.10. 1 hit.
1.20.1280.30. 1 hit.
InterProiIPR004222. Me_mOase_g.
IPR015952. Me_mOase_g_dom1.
IPR015953. Me_mOase_g_dom2.
[Graphical view]
PfamiPF02964. MeMO_Hyd_G. 1 hit.
[Graphical view]
PIRSFiPIRSF018503. Me_mOase_g. 1 hit.
ProDomiPD022203. Me_mOase_g. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47152. SSF47152. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKREPIHDN SIRTEWEAKI AKLTSVDQAT KFIQDFRLAY TSPFRKSYDI
60 70 80 90 100
DVDYQYIERK IEEKLSVLKT EKLPVADLIT KATTGEDRAA VEATWIAKIK
110 120 130 140 150
AAKSKYEADG IHIEFRQLYK PPVLPVNVFL RTDAALGTVL MEIRNTDYYG
160
TPLEGLRKEP GVKVLHLQA
Length:169
Mass (Da):19,326
Last modified:January 23, 2007 - v3
Checksum:i460D4D8D234C2229
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55394 Genomic DNA. Translation: CAA39071.1.
PIRiS15210. C39049.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55394 Genomic DNA. Translation: CAA39071.1.
PIRiS15210. C39049.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MHYX-ray2.00G1-169[»]
1MHZX-ray2.70G1-169[»]
ProteinModelPortaliP27355.
SMRiP27355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi595536.MettrDRAFT_0738.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105J38. Bacteria.
ENOG4111P1K. LUCA.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3869.

Miscellaneous databases

EvolutionaryTraceiP27355.

Family and domain databases

Gene3Di1.20.1280.10. 1 hit.
1.20.1280.30. 1 hit.
InterProiIPR004222. Me_mOase_g.
IPR015952. Me_mOase_g_dom1.
IPR015953. Me_mOase_g_dom2.
[Graphical view]
PfamiPF02964. MeMO_Hyd_G. 1 hit.
[Graphical view]
PIRSFiPIRSF018503. Me_mOase_g. 1 hit.
ProDomiPD022203. Me_mOase_g. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47152. SSF47152. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMEMG_METTR
AccessioniPrimary (citable) accession number: P27355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.