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P27354

- MEMB_METTR

UniProt

P27354 - MEMB_METTR

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Protein

Methane monooxygenase component A beta chain

Gene

mmoY

Organism
Methylosinus trichosporium
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds.

Catalytic activityi

Methane + NAD(P)H + O2 = methanol + NAD(P)+ + H2O.

GO - Molecular functioni

  1. methane monooxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular aromatic compound metabolic process Source: InterPro
  2. one-carbon metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3868.

Names & Taxonomyi

Protein namesi
Recommended name:
Methane monooxygenase component A beta chain (EC:1.14.13.25)
Alternative name(s):
Methane hydroxylase
Gene namesi
Name:mmoY
OrganismiMethylosinus trichosporium
Taxonomic identifieri426 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylocystaceaeMethylosinus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 394393Methane monooxygenase component A beta chainPRO_0000096408Add
BLAST

Interactioni

Subunit structurei

M.trichosporium has two forms of methane monooxygenase, a soluble and a membrane-bound type. The soluble type consists of four components (A to D): protein A, comprising three chains, in an alpha-2, beta-2, gamma-2 configuration, is a nonheme iron protein containing an unusual mu-hydroxo bridge structure at its active site and interacts with both oxygen and methane.

Structurei

Secondary structure

1
394
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni13 – 153
Helixi17 – 2610
Turni38 – 414
Beta strandi45 – 484
Helixi51 – 566
Helixi88 – 903
Helixi98 – 1003
Helixi109 – 13325
Helixi135 – 1384
Helixi141 – 1455
Helixi148 – 17225
Helixi176 – 20631
Helixi215 – 2239
Helixi225 – 2273
Helixi228 – 23912
Helixi244 – 25310
Helixi255 – 26410
Turni265 – 2673
Helixi268 – 2725
Turni273 – 2764
Helixi280 – 30223
Helixi303 – 3075
Turni310 – 3123
Helixi313 – 33826
Helixi339 – 3446
Helixi364 – 3685
Helixi373 – 3753
Helixi381 – 3899

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MHYX-ray2.00B1-394[»]
1MHZX-ray2.70B1-394[»]
ProteinModelPortaliP27354.
SMRiP27354. Positions 11-392.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27354.

Family & Domainsi

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012078. MP_mOase_hydro.
IPR003430. Phenol_Hydrox.
IPR012348. RNR-rel.
[Graphical view]
PfamiPF02332. Phenol_Hydrox. 1 hit.
[Graphical view]
PIRSFiPIRSF000040. MMOH_comp. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27354-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQPQSSQVT KRGLTDPERA AIIAAAVPDH ALDTQRKYHY FIQPRWKPLS
60 70 80 90 100
EYEQLSCYAQ PNPDWIAGGL DWGDWTQKFH GGRPSWGNES TELRTTDWYR
110 120 130 140 150
HRDPARRWHH PYVKDKSEEA RYTQRFLAAY SSEGSIRTID PYWRDEILNK
160 170 180 190 200
YFGALLYSEY GLFNAHSSVG RDCLSDTIRQ TAVFAALDKV DNAQMIQMER
210 220 230 240 250
LFIAKLVPGF DASTDVPKKI WTTDPIYSGA RATVQEIWQG VQDWNEILWA
260 270 280 290 300
GHAVMIATFG QFARREFFQR LATVYGDTLT PFFTAQSQTY FQTTRGAIDD
310 320 330 340 350
LFVYCLANDS EFGAHNRTFL NAWTEHYLAS SVAALKDFVG LYAKVEKSRA
360 370 380 390
DRSRRRLRGA AASSAIGRSI TPDKIGFRVD VDQKVDAVLA GYKN
Length:394
Mass (Da):45,020
Last modified:January 23, 2007 - v3
Checksum:iFA6C8F0970F28B90
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55394 Genomic DNA. Translation: CAA39069.1.
PIRiS15208.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55394 Genomic DNA. Translation: CAA39069.1 .
PIRi S15208.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MHY X-ray 2.00 B 1-394 [» ]
1MHZ X-ray 2.70 B 1-394 [» ]
ProteinModelPortali P27354.
SMRi P27354. Positions 11-392.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-3868.

Miscellaneous databases

EvolutionaryTracei P27354.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012078. MP_mOase_hydro.
IPR003430. Phenol_Hydrox.
IPR012348. RNR-rel.
[Graphical view ]
Pfami PF02332. Phenol_Hydrox. 1 hit.
[Graphical view ]
PIRSFi PIRSF000040. MMOH_comp. 1 hit.
SUPFAMi SSF47240. SSF47240. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b."
    Cardy D.L.N., Laidler V., Salmond G.P.C., Murrell J.C.
    Mol. Microbiol. 5:335-342(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: OB3b.
  2. "Complex formation between the protein components of methane monooxygenase from Methylosinus trichosporium OB3b. Identification of sites of component interaction."
    Fox B.G., Liu Y., Dege J.E., Lipscomb J.D.
    J. Biol. Chem. 266:540-550(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-16.

Entry informationi

Entry nameiMEMB_METTR
AccessioniPrimary (citable) accession number: P27354
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 77 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3