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P27353

- MEMA_METTR

UniProt

P27353 - MEMA_METTR

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Protein

Methane monooxygenase component A alpha chain

Gene
mmoX
Organism
Methylosinus trichosporium
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds.

Catalytic activityi

Methane + NAD(P)H + O2 = methanol + NAD(P)+ + H2O.

Cofactori

Binds 2 iron ions.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi114 – 1141Iron 1 By similarity
Metal bindingi144 – 1441Iron 1 By similarity
Metal bindingi147 – 1471Iron 1 By similarity
Active sitei151 – 1511 Reviewed prediction
Metal bindingi209 – 2091Iron 2 By similarity
Metal bindingi243 – 2431Iron 2 By similarity
Metal bindingi246 – 2461Iron 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. methane monooxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular aromatic compound metabolic process Source: InterPro
  2. one-carbon metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Methane monooxygenase component A alpha chain (EC:1.14.13.25)
Alternative name(s):
Methane hydroxylase
Gene namesi
Name:mmoX
OrganismiMethylosinus trichosporium
Taxonomic identifieri426 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylocystaceaeMethylosinus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 526525Methane monooxygenase component A alpha chainPRO_0000096406Add
BLAST

Interactioni

Subunit structurei

M.trichosporium has two forms of methane monooxygenase, a soluble and a membrane-bound type. The soluble type consists of four components (A to D): protein A, comprising three chains, in an alpha-2, beta-2, gamma-2 configuration, is a nonheme iron protein containing an unusual mu-hydroxo bridge structure at its active site and interacts with both oxygen and methane.

Structurei

Secondary structure

1
526
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi25 – 295
Helixi30 – 356
Helixi64 – 8320
Helixi85 – 884
Helixi91 – 933
Helixi97 – 12731
Helixi131 – 16131
Turni166 – 1705
Helixi171 – 1744
Helixi175 – 1773
Helixi179 – 18810
Helixi190 – 1934
Helixi197 – 2048
Turni205 – 2073
Helixi208 – 2114
Helixi213 – 22614
Helixi231 – 25727
Helixi261 – 29232
Helixi301 – 3088
Helixi309 – 3157
Helixi316 – 3205
Helixi321 – 3244
Helixi332 – 3387
Turni339 – 3413
Helixi342 – 35211
Helixi354 – 3563
Beta strandi357 – 3604
Helixi366 – 37510
Turni377 – 3826
Helixi383 – 39311
Turni394 – 3963
Helixi398 – 4003
Helixi404 – 4107
Turni419 – 4213
Turni427 – 4293
Beta strandi437 – 4415
Beta strandi444 – 4485
Helixi451 – 4599
Helixi461 – 4633
Helixi469 – 4735
Helixi478 – 4847
Beta strandi492 – 4965
Beta strandi498 – 5003
Beta strandi502 – 5043
Helixi509 – 5146
Helixi522 – 5254

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MHYX-ray2.00D1-525[»]
1MHZX-ray2.70D1-525[»]
ProteinModelPortaliP27353.
SMRiP27353. Positions 17-526.

Miscellaneous databases

EvolutionaryTraceiP27353.

Family & Domainsi

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR003430. Phenol_Hydrox.
IPR012348. RNR-rel.
[Graphical view]
PfamiPF02332. Phenol_Hydrox. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27353-1 [UniParc]FASTAAdd to Basket

« Hide

MAISLATKAA TDALKVNRAP VGVEPQEVHK WLQSFNWDFK ENRTKYPTKY    50
HMANETKEQF KVIAKEYARM EAAKDERQFG TLLDGLTRLG AGNKVHPRWG 100
ETMKVISNFL EVGEYNAIAA SAMLWDSATA AEQKNGYLAQ VLDEIRHTHQ 150
CAFINHYYSK HYHDPAGHND ARRTRAIGPL WKGMKRVFAD GFISGDAVEC 200
SVNLQLVGEA CFTNPLIVAV TEWASANGDE ITPTVFLSVE TDELRHMANG 250
YQTVVSIAND PASAKFLNTD LNNAFWTQQK YFTPVLGYLF EYGSKFKVEP 300
WVKTWNRWVY EDWGGIWIGR LGKYGVESPA SLRDAKRDAY WAHHDLALAA 350
YAMWPLGFAR LALPDEEDQA WFEANYPGWA DHYGKIFNEW KKLGYEDPKS 400
GFIPYQWLLA NGHDVYIDRV SQVPFIPSLA KGTGSLRVHE FNGKKHSLTD 450
DWGERQWLIE PERYECHNVF EQYEGRELSE VIAEGHGVRS DGKTLIAQPH 500
TRGDNLWTLE DIKRAGCVFP DPLAKF 526
Length:526
Mass (Da):59,954
Last modified:January 23, 2007 - v4
Checksum:iA56F4AAD0A55726B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55394 Genomic DNA. Translation: CAA39068.2.
PIRiS15207.

Genome annotation databases

PATRICi38005870. VBIMetTri17036_0736.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55394 Genomic DNA. Translation: CAA39068.2 .
PIRi S15207.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MHY X-ray 2.00 D 1-525 [» ]
1MHZ X-ray 2.70 D 1-525 [» ]
ProteinModelPortali P27353.
SMRi P27353. Positions 17-526.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

PATRICi 38005870. VBIMetTri17036_0736.

Miscellaneous databases

EvolutionaryTracei P27353.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR003430. Phenol_Hydrox.
IPR012348. RNR-rel.
[Graphical view ]
Pfami PF02332. Phenol_Hydrox. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b."
    Cardy D.L.N., Laidler V., Salmond G.P.C., Murrell J.C.
    Mol. Microbiol. 5:335-342(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: OB3b.
  2. McDonald I.
    Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Complex formation between the protein components of methane monooxygenase from Methylosinus trichosporium OB3b. Identification of sites of component interaction."
    Fox B.G., Liu Y., Dege J.E., Lipscomb J.D.
    J. Biol. Chem. 266:540-550(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-16.

Entry informationi

Entry nameiMEMA_METTR
AccessioniPrimary (citable) accession number: P27353
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: December 11, 2013
This is version 82 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

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