AP-2 complex subunit beta - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P27351 (AP2B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
AP-2 complex subunit beta

Alternative name(s):
Beta-2-adaptin
Beta-adaptin
Clathrin assembly protein complex 2 beta large chain
Clathrin assembly protein large beta chain

Gene names

Name:	APL1
Synonyms:	YAP80
Ordered Locus Names:	YJR005W
ORF Names:	J1422

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	700 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Beta adaptin is a subunit of the plasma membrane adaptor. Ref.4
Subunit structure	Assembly protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit APL3 and beta-type subunit APL1), a medium chain (mu-type subunit APM4) and a small adaptin (sigma-type subunit APS2). Interacts with APS2. Ref.4
Subcellular location	Cell membrane. Membrane › coated pit; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.
Miscellaneous	Present with 1670 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the adaptor complexes large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Coated pit Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Traceable author statement. Source: SGD
Cellular component	coated pit Inferred from electronic annotation. Source: UniProtKB-SubCell membrane coat Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 700

700

AP-2 complex subunit beta

PRO_0000193755

Amino acid modifications

Modified residue

649

Phosphoserine Ref.6

Modified residue

652

Phosphothreonine Ref.6

Modified residue

683

Phosphoserine Ref.6

Modified residue

687

Phosphoserine Ref.6 Ref.7

Experimental info

Sequence conflict

V → L in AAA35226. Ref.1

Sequence conflict

484

S → N in AAA35226. Ref.1

Sequence conflict

504

S → N in AAA35226. Ref.1

Sequence conflict

521

I → T in AAA35226. Ref.1

Sequence conflict

629

F → I in AAA35226. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P27351 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 556A8F3EF8B3930F
Show »

FASTA

700

80,453

        10         20         30         40         50         60 
MSDQKVFARY KANEIVTDLQ HFGVKKFKSN ITRRKNALRK IIANLVLGNY GEMSVLFSEL 

        70         80         90        100        110        120 
LKFWQIEDDL EVKRICHEYI RVIGALKPQQ AREALPFIMD DFKSRDEKLQ IMALRTLVLV 

       130        140        150        160        170        180 
PVKELSDQAF DCIISLVNHK SPPEQVTRTA IYALLDLDEI DHERVLGLSS ILHDIVKAQS 

       190        200        210        220        230        240 
SSPEVIVAAL HTLYSIHEKN ANMEPFRIPL ELAFDMLELL PELNEWNKAT VLEVLTTSVV 

       250        260        270        280        290        300 
PQHYLDTHEM IELALPYLQQ VNTYVVLNSL KFIMYLLNYV DVIKETLAEK LSNSVIALLD 

       310        320        330        340        350        360 
KPPELQFLVL RNVILLLLSR ESSLLRLDIS YFFIEYNDPI YIKDTKLECL YLLANKETLP 

       370        380        390        400        410        420 
RILEELEQYA TDIDIQMSRK SVRAIGNLAV KLDEDSVHDC VAVLLDLLEF GVDYVVQEII 

       430        440        450        460        470        480 
SVFRNILRKY PNNFKANVTE LVKHTEVVQE PESKNAMIWI ITQYSDVIPN YLELFRVFSS 

       490        500        510        520        530        540 
NMFSETLEVQ FSILNSAIKF FIRSPTKETE ELCMDLLKGC IDHENNPDLR DKTLMYWRLL 

       550        560        570        580        590        600 
SLTKTSRISN AITFESLKSV LDGELPLIEM NTKLDPTVLE ELELNIGTIV SIYLKPVSHI 

       610        620        630        640        650        660 
FRLNKTKLLP QSPILNPNKD LLPVVGNSFP PTGANRDRQN SESQSSTKSR KTAMMDDYDK 

       670        680        690        700 
PAEKINQLKG KRKSSSNNPS KLSRKPSTLL RKLSMKRPFS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a putative yeast homolog of the mammalian beta chains of the clathrin-associated protein complexes." Kirchhausen T. Mol. Cell. Biol. 10:6089-6090(1990) [PubMed: 2122239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Adaptor complex-independent clathrin function in yeast." Yeung B.G., Phan H.L., Payne G.S. Mol. Biol. Cell 10:3643-3659(1999) [PubMed: 10564262] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH APS2.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-649; THR-652; SER-683 AND SER-687, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-687, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64998 Genomic DNA. Translation: AAA35226.1. X87611 Genomic DNA. Translation: CAA60927.1. Sequence problems. Z49505 Genomic DNA. Translation: CAA89527.1. BK006943 Genomic DNA. Translation: DAA08795.1.
PIR	S57020.
RefSeq	NP_012538.1. NM_001181662.1.
3D structure databases
ProteinModelPortal	P27351.
SMR	P27351. Positions 4-601.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-915N.
IntAct	P27351. 4 interactions.
MINT	MINT-504477.
STRING	P27351.
Proteomic databases
PeptideAtlas	P27351.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR005W; YJR005W; YJR005W.
GeneID	853461.
KEGG	sce:YJR005W.
NMPDR	fig\|4932.3.peg.3511.
Organism-specific databases
CYGD	YJR005w.
SGD	S000003765. APL1.
Phylogenomic databases
eggNOG	fuNOG04097.
HOGENOM	HBG718694.
OMA	VLKWATE.
OrthoDB	EOG476P7N.
Gene expression databases
ArrayExpress	P27351.
Genevestigator	P27351.
GermOnline	YJR005W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016342. AP_complex_bsu. IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR002553. Clathrin/coatomer_adapt-like_N. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF01602. Adaptin_N. 1 hit. [Graphical view]
PIRSF	PIRSF002291. AP_complex_beta. 1 hit.
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	974041.

Entry information

Entry name

AP2B_YEAST

Accession

Primary (citable) accession number: P27351
Secondary accession number(s): D6VWH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents