Reviewed, UniProtKB/Swiss-Prot P27351 (AP2B_YEAST)

Names and origin

Protein names

Recommended name:
    AP-2 complex subunit beta
Alternative name(s):
    Beta2-adaptin
    Beta-adaptin
    Clathrin assembly protein complex 2 beta large chain
    Clathrin assembly protein large beta chain

Gene names

Name:	APL1
Synonyms:	YAP80
Ordered Locus Names:	YJR005W
ORF Names:	J1422

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	700 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Beta adaptin is a subunit of the plasma membrane adaptor. Ref.3
Subunit structure	Assembly protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit APL3 and beta-type subunit APL1), a medium chain (mu-type subunit APM4) and a small adaptin (sigma-type subunit APS2). Interacts with APS2. Ref.3
Subcellular location	Cell membrane. Membrane › coated pit; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.
Miscellaneous	Present with 1670 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the adaptor complexes large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Coated pit Membrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Traceable author statement. Source: SGD
Cellular component	coated pit Inferred from electronic annotation. Source: UniProtKB-KW membrane coat Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.3 Inferred from physical interaction. Source: IntAct protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-2202,EBI-24597
APS2	Q00381	1	EBI-2202,EBI-2608

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 700	700	AP-2 complex subunit beta		PRO_0000193755
Amino acid modifications
	Modified residue	649	1	Phosphoserine Ref.5
	Modified residue	652	1	Phosphothreonine Ref.5
	Modified residue	683	1	Phosphoserine Ref.5
	Modified residue	687	1	Phosphoserine Ref.5 Ref.6
Experimental info
	Sequence conflict	55	1	V → L in AAA35226. Ref.1
	Sequence conflict	484	1	S → N in AAA35226. Ref.1
	Sequence conflict	504	1	S → N in AAA35226. Ref.1
	Sequence conflict	521	1	I → T in AAA35226. Ref.1
	Sequence conflict	629	1	F → I in AAA35226. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P27351-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 556A8F3EF8B3930F	FASTA	700	80,453
	10 20 30 40 50 60 MSDQKVFARY KANEIVTDLQ HFGVKKFKSN ITRRKNALRK IIANLVLGNY GEMSVLFSEL 70 80 90 100 110 120 LKFWQIEDDL EVKRICHEYI RVIGALKPQQ AREALPFIMD DFKSRDEKLQ IMALRTLVLV 130 140 150 160 170 180 PVKELSDQAF DCIISLVNHK SPPEQVTRTA IYALLDLDEI DHERVLGLSS ILHDIVKAQS 190 200 210 220 230 240 SSPEVIVAAL HTLYSIHEKN ANMEPFRIPL ELAFDMLELL PELNEWNKAT VLEVLTTSVV 250 260 270 280 290 300 PQHYLDTHEM IELALPYLQQ VNTYVVLNSL KFIMYLLNYV DVIKETLAEK LSNSVIALLD 310 320 330 340 350 360 KPPELQFLVL RNVILLLLSR ESSLLRLDIS YFFIEYNDPI YIKDTKLECL YLLANKETLP 370 380 390 400 410 420 RILEELEQYA TDIDIQMSRK SVRAIGNLAV KLDEDSVHDC VAVLLDLLEF GVDYVVQEII 430 440 450 460 470 480 SVFRNILRKY PNNFKANVTE LVKHTEVVQE PESKNAMIWI ITQYSDVIPN YLELFRVFSS 490 500 510 520 530 540 NMFSETLEVQ FSILNSAIKF FIRSPTKETE ELCMDLLKGC IDHENNPDLR DKTLMYWRLL 550 560 570 580 590 600 SLTKTSRISN AITFESLKSV LDGELPLIEM NTKLDPTVLE ELELNIGTIV SIYLKPVSHI 610 620 630 640 650 660 FRLNKTKLLP QSPILNPNKD LLPVVGNSFP PTGANRDRQN SESQSSTKSR KTAMMDDYDK 670 680 690 700 PAEKINQLKG KRKSSSNNPS KLSRKPSTLL RKLSMKRPFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a putative yeast homolog of the mammalian beta chains of the clathrin-associated protein complexes." Kirchhausen T. Mol. Cell. Biol. 10:6089-6090(1990) [PubMed: 2122239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Adaptor complex-independent clathrin function in yeast." Yeung B.G., Phan H.L., Payne G.S. Mol. Biol. Cell 10:3643-3659(1999) [PubMed: 10564262] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH APS2.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-649; THR-652; SER-683 AND SER-687, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-687, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M64998 Genomic DNA. Translation: AAA35226.1. X87611 Genomic DNA. Translation: CAA60927.1. Sequence problems. Z49505 Genomic DNA. Translation: CAA89527.1.
PIR	S57020.
RefSeq	NP_012538.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:915N.
IntAct	P27351. 16 interactions.
STRING	P27351.
Proteomic databases
PeptideAtlas	P27351.
Genome annotation databases
Ensembl	YJR005W; YJR005W; YJR005W; Saccharomyces cerevisiae. [Genome view]
GeneID	853461.
KEGG	sce:YJR005W.
NMPDR	fig|4932.3.peg.3511.
Organism-specific databases
CYGD	YJR005w.
SGD	S000003765. APL1.
Phylogenomic databases
HOGENOM	P27351.
OMA	VANMTMS
OrthoDB	EOG9HHQK0
Gene expression databases
ArrayExpress	P27351.
Genevestigator	P27351.
GermOnline	YJR005W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016342. AP_complex_bsu. IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR002553. Clathrin/coatomer_adapt-like_N. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF01602. Adaptin_N. 1 hit. [Graphical view]
PIRSF	PIRSF002291. AP_complex_beta. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	974041.

Entry information

Entry name

AP2B_YEAST

Accession

Primary (citable) accession number: P27351

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)