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Protein

14-3-3 protein theta

Gene

YWHAQ

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.1 Publication

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-111447. Activation of BAD and translocation to mitochondria.
R-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-5625740. RHO GTPases activate PKNs.
R-HSA-5628897. TP53 Regulates Metabolic Genes.
R-HSA-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
SignaLinkiP27348.
SIGNORiP27348.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein theta
Alternative name(s):
14-3-3 protein T-cell
14-3-3 protein tau
Protein HS1
Gene namesi
Name:YWHAQ
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:12854. YWHAQ.

Subcellular locationi

  • Cytoplasm

  • Note: In neurons, axonally transported to the nerve terminals.

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • cytoplasmic vesicle membrane Source: Reactome
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • focal adhesion Source: UniProtKB
  • membrane Source: UniProtKB
  • mitochondrion Source: GOC
  • protein complex Source: MGI

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi10971.
OpenTargetsiENSG00000134308.
PharmGKBiPA37443.

Chemistry databases

ChEMBLiCHEMBL3710408.

Polymorphism and mutation databases

DMDMi112690.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586361 – 24514-3-3 protein thetaAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei3N6-acetyllysineCombined sources1
Modified residuei49N6-acetyllysine; alternateCombined sources1
Cross-linki49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei68N6-acetyllysineCombined sources1
Modified residuei82Nitrated tyrosineBy similarity1
Modified residuei92PhosphoserineBy similarity1
Modified residuei104Nitrated tyrosineBy similarity1
Modified residuei115N6-acetyllysineCombined sources1
Modified residuei232PhosphoserineCombined sources1 Publication1

Post-translational modificationi

Ser-232 is probably phosphorylated by CK1.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Nitration, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP27348.
MaxQBiP27348.
PaxDbiP27348.
PeptideAtlasiP27348.
PRIDEiP27348.
TopDownProteomicsiP27348.

2D gel databases

OGPiP27348.
REPRODUCTION-2DPAGEiIPI00018146.

PTM databases

iPTMnetiP27348.
PhosphoSitePlusiP27348.
SwissPalmiP27348.

Miscellaneous databases

PMAP-CutDBiP27348.

Expressioni

Tissue specificityi

Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.1 Publication

Gene expression databases

BgeeiENSG00000134308.
CleanExiHS_YWHAQ.
ExpressionAtlasiP27348. baseline and differential.
GenevisibleiP27348. HS.

Organism-specific databases

HPAiCAB010286.
HPA007925.

Interactioni

Subunit structurei

Homodimer. Interacts with CDK16 (By similarity). Interacts with RGS7 (phosphorylated form) (PubMed:10862767). Interacts with SSH1. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with GAB2. Interacts with the 'Ser-241' phosphorylated form of PDPK1. Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (PubMed:23572552). Interacts with SLITRK1 (PubMed:19640509). Interacts with RIPOR2 isoform 2 (PubMed:25588844).By similarity10 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei56Interaction with phosphoserine on interacting protein1
Sitei127Interaction with phosphoserine on interacting protein1

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116168. 447 interactors.
DIPiDIP-27584N.
ELMiP27348.
IntActiP27348. 111 interactors.
MINTiMINT-121282.
STRINGi9606.ENSP00000238081.

Structurei

Secondary structure

1245
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 15Combined sources13
Helixi19 – 30Combined sources12
Helixi38 – 67Combined sources30
Helixi74 – 103Combined sources30
Turni104 – 108Combined sources5
Helixi112 – 132Combined sources21
Helixi136 – 159Combined sources24
Helixi165 – 180Combined sources16
Helixi185 – 201Combined sources17
Helixi202 – 205Combined sources4
Turni208 – 210Combined sources3
Helixi211 – 229Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BTPX-ray2.80A/B1-234[»]
5IQPX-ray2.60A/B1-245[»]
ProteinModelPortaliP27348.
SMRiP27348.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27348.

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP27348.
KOiK16197.
OMAiWTSDNAT.
OrthoDBiEOG091G0VKY.
PhylomeDBiP27348.
TreeFamiTF102002.

Family and domain databases

Gene3Di1.20.190.20. 1 hit.
InterProiView protein in InterPro
IPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiView protein in Pfam
PF00244. 14-3-3. 1 hit.
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiView protein in SMART
SM00101. 14_3_3. 1 hit.
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiView protein in PROSITE
PS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P27348-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN
60 70 80 90 100
VVGGRRSAWR VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL
110 120 130 140 150
LDKYLIANAT NPESKVFYLK MKGDYFRYLA EVACGDDRKQ TIDNSQGAYQ
160 170 180 190 200
EAFDISKKEM QPTHPIRLGL ALNFSVFYYE ILNNPELACT LAKTAFDEAI
210 220 230 240
AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN
Length:245
Mass (Da):27,764
Last modified:August 1, 1992 - v1
Checksum:i175534325E9E37C4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136D → N in AAV38817 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56468 mRNA. Translation: CAA39840.1.
X57347 mRNA. Translation: CAA40622.1.
BT020014 mRNA. Translation: AAV38817.1.
CH471053 Genomic DNA. Translation: EAX00977.1.
CH471053 Genomic DNA. Translation: EAX00979.1.
BC050601 mRNA. Translation: AAH50601.1.
BC056867 mRNA. Translation: AAH56867.1.
BC093019 mRNA. Translation: AAH93019.1.
AF070556 mRNA. Translation: AAC28640.1.
CCDSiCCDS1666.1.
PIRiS15076.
RefSeqiNP_006817.1. NM_006826.3.
UniGeneiHs.74405.

Genome annotation databases

EnsembliENST00000238081; ENSP00000238081; ENSG00000134308.
ENST00000381844; ENSP00000371267; ENSG00000134308.
GeneIDi10971.
KEGGihsa:10971.
UCSCiuc002qzx.5. human.

Similar proteinsi

Entry informationi

Entry namei1433T_HUMAN
AccessioniPrimary (citable) accession number: P27348
Secondary accession number(s): D6W4Z5
, Q567U5, Q5TZU8, Q9UP48
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: August 30, 2017
This is version 192 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families