Reviewed, UniProtKB/Swiss-Prot P27348 (1433T_HUMAN)

Names and origin

Protein names

Recommended name:
    14-3-3 protein theta
Alternative name(s):
    14-3-3 protein tau
    14-3-3 protein T-cell
    Protein HS1

Gene names

Name:

YWHAQ

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Subunit structure	Homodimer. Interacts with PCTK1 By similarity. Interacts with SSH1. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm.
Subcellular location	Cytoplasm. Note: In neurons, axonally transported to the nerve terminals.
Tissue specificity	Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement. Ref.9
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of transcription, DNA-dependent Inferred from direct assay. Source: UniProtKB
Cellular component	centrosome Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Molecular function	protein N-terminus binding Inferred from physical interaction. Source: UniProtKB protein domain specific binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADAM22	Q9P0K1-1	2	EBI-359854,EBI-1567258
ADAM22	Q9P0K1-3	1	EBI-359854,EBI-1567267
ARRB1	P49407	1	EBI-359854,EBI-743313
ARRB2	P32121	1	EBI-359854,EBI-714559
BRAF	P15056	1	EBI-359854,EBI-365980
C22orf9	Q6ICG6	1	EBI-359854,EBI-1053969
CALML5	Q9NZT1	1	EBI-359854,EBI-1051681
CBWD2	Q8IUF1	1	EBI-359854,EBI-359159
DDX6	P26196	1	EBI-359854,EBI-351257
DENND4A	Q7Z401	1	EBI-359854,EBI-1046479
EDC3	Q96F86	1	EBI-359854,EBI-997311
EML3	Q32P44	1	EBI-359854,EBI-1046713
EPB41L2	O43491	1	EBI-359854,EBI-1052044
EPB41L3	Q9Y2J2	1	EBI-359854,EBI-310986
FSHR	P23945	3	EBI-359854,EBI-848239
FUSIP1	O75494	1	EBI-359854,EBI-353655
IRS2	Q9Y4H2	1	EBI-359854,EBI-1049582
KIF23	Q02241	1	EBI-359854,EBI-306852
KIF5B	P33176	1	EBI-359854,EBI-355878
KIF5C	O60282	1	EBI-359854,EBI-717170
KLC2	Q9H0B6	1	EBI-359854,EBI-726994
KLC4	Q9NSK0	1	EBI-359854,EBI-949319
LARP1	Q6PKG0	1	EBI-359854,EBI-1052114
LUC7L2	Q9Y383	1	EBI-359854,EBI-352851
MAGOH	P61326	1	EBI-359854,EBI-299134
MARK3	P27448	1	EBI-359854,EBI-707595
MPRIP	Q6WCQ1	1	EBI-359854,EBI-1022605
PAK4	O96013	1	EBI-359854,EBI-713738
PAK6	Q9NQU5	1	EBI-359854,EBI-1053685
PFTK1	O94921	3	EBI-359854,EBI-1043945
PI4KB	Q9UBF8	1	EBI-359854,EBI-1053214
PNKP	Q96T60	1	EBI-359854,EBI-1045072
PPFIBP1	Q86W92	1	EBI-359854,EBI-1045582
RABEP1	Q15276	1	EBI-359854,EBI-447043
RAF1	P04049	1	EBI-359854,EBI-365996
RAI14	Q9P0K7	1	EBI-359854,EBI-1023749
RASGRP3	Q8IV61	1	EBI-359854,EBI-1047876
SAMD4B	Q5PRF9	1	EBI-359854,EBI-1047489
SFRS1	Q07955	1	EBI-359854,EBI-398920
SFRS3	P84103	1	EBI-359854,EBI-372557
SFRS6	Q13247	1	EBI-359854,EBI-745230
SH3BP4	Q9P0V3	1	EBI-359854,EBI-1049513
SPIRE1	Q08AE8	1	EBI-359854,EBI-1055655
SUV420H1	Q4FZB7	1	EBI-359854,EBI-1047962
TJP2	Q9UDY2	1	EBI-359854,EBI-1042602
TSC2	P49815	1	EBI-359854,EBI-396587
USP8	P40818	1	EBI-359854,EBI-1050865

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 245

245

14-3-3 protein theta

PRO_0000058636

Sites

Site

56

1

Interaction with phosphoserine on interacting protein

Site

127

1

Interaction with phosphoserine on interacting protein

Amino acid modifications

Modified residue

1

1

N-acetylmethionine Ref.6

Modified residue

232

1

Phosphoserine; by CK1 Probable

Experimental info

Sequence conflict

136

1

D → N in AAV38817. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

245

Helix Strand Turn

Details...

Helix

3 – 15

13

Helix

19 – 31

13

Helix

38 – 68

31

Helix

75 – 103

29

Turn

104 – 108

5

Helix

112 – 132

21

Helix

135 – 159

25

Helix

165 – 180

16

Helix

185 – 201

17

Turn

202 – 205

4

Helix

208 – 227

20

Sequences

	Sequence		Length	Mass (Da)
	P27348-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 175534325E9E37C4	FASTA	245	27,764
	10 20 30 40 50 60 MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN VVGGRRSAWR 70 80 90 100 110 120 VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL LDKYLIANAT NPESKVFYLK 130 140 150 160 170 180 MKGDYFRYLA EVACGDDRKQ TIDNSQGAYQ EAFDISKKEM QPTHPIRLGL ALNFSVFYYE 190 200 210 220 230 240 ILNNPELACT LAKTAFDEAI AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of a human protein kinase regulator protein." Nielsen P.J. Biochim. Biophys. Acta 1088:425-428(1991) [PubMed: 2015305] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: T-cell.
[2]	"Molecular cloning and expression of the transformation sensitive epithelial marker stratifin. A member of a protein family that has been involved in the protein kinase C signalling pathway." Leffers H., Madsen P., Rasmussen H.H., Honore B., Andersen A.H., Walbum E., Vandekerckhove J., Celis J.E. J. Mol. Biol. 231:982-998(1993) [PubMed: 8515476] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Keratinocyte.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye, Skin and Uterus.
[5]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-18. Tissue: Platelet.
[6]	Bienvenut W.V., Dhillon A.S., Kolch W. Submitted (FEB-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-9; 28-49; 61-68; 104-115; 139-167 AND 213-222, ACETYLATION AT MET-1, MASS SPECTROMETRY. Tissue: B-cell lymphoma and Hepatoma.
[7]	Yu W., Gibbs R.A. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 73-245. Tissue: Brain.
[8]	"14-3-3 is phosphorylated by casein kinase I on residue 233. Phosphorylation at this site in vivo regulates Raf/14-3-3 interaction." Dubois T., Rommel C., Howell S., Steinhussen U., Soneji Y., Morrice N., Moelling K., Aitken A. J. Biol. Chem. 272:28882-28888(1997) [PubMed: 9360956] [Abstract] Cited for: PHOSPHORYLATION AT SER-232, MASS SPECTROMETRY.
[9]	"A 14-3-3 mRNA is up-regulated in amyotrophic lateral sclerosis spinal cord." Malaspina A., Kaushik N., de Belleroche J. J. Neurochem. 75:2511-2520(2000) [PubMed: 11080204] [Abstract] Cited for: TISSUE SPECIFICITY.
[10]	"Akt-dependent phosphorylation of p27Kip1 promotes binding to 14-3-3 and cytoplasmic localization." Fujita N., Sato S., Katayama K., Tsuruo T. J. Biol. Chem. 277:28706-28713(2002) [PubMed: 12042314] [Abstract] Cited for: INTERACTION WITH CDKN1B.
[11]	"A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia." Nagata-Ohashi K., Ohta Y., Goto K., Chiba S., Mori R., Nishita M., Ohashi K., Kousaka K., Iwamatsu A., Niwa R., Uemura T., Mizuno K. J. Cell Biol. 165:465-471(2004) [PubMed: 15159416] [Abstract] Cited for: INTERACTION WITH SSH1.
[12]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232, MASS SPECTROMETRY.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232, MASS SPECTROMETRY.
[14]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[15]	"Structure of a 14-3-3 protein and implications for coordination of multiple signalling pathways." Xiao B., Smerdon S.J., Jones D.H., Dodson G.G., Soneji Y., Aitken A., Gamblin S.J. Nature 376:188-191(1995) [PubMed: 7603573] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
[16]	"Structural basis for protein-protein interactions in the 14-3-3 protein family." Yang X., Lee W.H., Sobott F., Papagrigoriou E., Robinson C.V., Grossmann J.G., Sundstroem M., Doyle D.A., Elkins J.M. Proc. Natl. Acad. Sci. U.S.A. 103:17237-17242(2006) [PubMed: 17085597] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 1-234, MASS SPECTROMETRY, INTERACTION WITH PHOSPHOSERINE MOTIFS, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X56468 mRNA. Translation: CAA39840.1.
X57347 mRNA. Translation: CAA40622.1.
BT020014 mRNA. Translation: AAV38817.1.
BC001197 mRNA. Translation: AAH01197.1.
BC050601 mRNA. Translation: AAH50601.1.
BC056867 mRNA. Translation: AAH56867.1.
BC093019 mRNA. Translation: AAH93019.1.
AF070556 mRNA. Translation: AAC28640.1.

IPI

IPI00018146.

PIR

S15076.

RefSeq

NP_006817.1.

UniGene

Hs.74405

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BTP	X-ray	2.80	A/B	1-234	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:27584N.

IntAct

P27348. 68 interactions.

PTM databases

PhosphoSite

P27348.

2-D gel databases

Cornea-2DPAGE

P27348.

OGP

P27348.

REPRODUCTION-2DPAGE

IPI00018146.

Proteomic databases

PeptideAtlas

P27348.

PRIDE

P27348.

Genome annotation databases

Ensembl

ENSG00000134308. Homo sapiens. [Contig view]

GeneID

10971.

KEGG

hsa:10971.

Organism-specific databases

GeneCards

GC02M009674.

H-InvDB

HIX0029798.
HIX0057400.

HGNC

HGNC:12854. YWHAQ.

HPA

CAB010286.
HPA007925.

MIM

609009. gene.

PharmGKB

PA37443.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P27348.

HOVERGEN

P27348.

OMA

P27348. DTSDKKM.

Enzyme and pathway databases

Pathway_Interaction_DB

a6b1_a6b4_integrin_pathway. a6b1 and a6b4 Integrin signaling.
pi3kciaktpathway. Class I PI3K signaling events mediated by Akt.
foxopathway. FoxO family signaling.
insulin_glucose_pathway. Insulin-mediated glucose transport.
p38_mk2pathway. p38 signaling mediated by MAPKAP kinases.
nfat_3pathway. Role of Calcineurin-dependent NFAT signaling in lymphocytes.
pi3kplctrkpathway. Trk receptor signaling mediated by PI3K and PLC-gamma.

Gene expression databases

ArrayExpress

P27348.

Bgee

P27348.

CleanEx

HS_YWHAQ.

GermOnline

ENSG00000134308. Homo sapiens.

Family and domain databases

InterPro

IPR000308. 14-3-3.
[Graphical view]

Gene3D

G3DSA:1.20.190.20. 14-3-3. 1 hit.

PANTHER

PTHR18860. 14-3-3. 1 hit.

Pfam

PF00244. 14-3-3. 1 hit.
[Graphical view]

PIRSF

PIRSF000868. 14-3-3. 1 hit.

PRINTS

PR00305. 1433ZETA.

ProDom

PD000600. 14-3-3. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00101. 14_3_3. 1 hit.
[Graphical view]

PROSITE

PS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

41686.

PMAP-CutDB

P27348.

SOURCE

Search...

Entry information

Entry name

1433T_HUMAN

Accession

Primary (citable) accession number: P27348
Secondary accession number(s): Q567U5, Q5TZU8, Q9UP48

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)