Reviewed, UniProtKB/Swiss-Prot P27346 (DHE2_CLODI)

Names and origin

Protein names

Recommended name:
    NAD-specific glutamate dehydrogenase
      Short name=NAD-GDH
    EC=1.4.1.2

Gene names

Name:

gluD

Organism

Clostridium difficile

Taxonomic identifier

1496 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD+ = 2-oxoglutarate + NH3 + NADH.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	NAD-specific glutamate dehydrogenase		PRO_0000182737
Sites
	Active site	107	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27346-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 31F0A09802F4AD94	FASTA	421	46,016
	10 20 30 40 50 60 MSGKDVNVFE MAQSQVKNAC DKLGMEPAVY ELLKEPMRVI EVSIPVKMDD GSIKTFKGFR 70 80 90 100 110 120 SQHNDAVGPT KGGIRFHQNV SRDEVKALSI WMTFKCSVTG IPYGGGKGGI IVDPSTLSQG 130 140 150 160 170 180 ELERLSRGYI DGIYKLIGEK VDVPAPDVNT NGQIMSWMVD EYNKLTGQSS IGVITGKPVE 190 200 210 220 230 240 FGGSLGRTAA TGFGVAVTAR EAAAKLGIDM KKAKIAVQGI GNVGSYTVLN CEKLGGTVVA 250 260 270 280 290 300 MAEWCKSEGS YAIYNENGLD GQAMLDYMKE HGNLLNFPGA KRISLEEFWA SDVDIVIPAA 310 320 330 340 350 360 LENSITKEVA ESIKAKLVCE AANGPTTPEA DEVFAERGIV LTPDILTNAG GVTVSYFEWV 370 380 390 400 410 420 QNLYGYYWSE EEVEQKEEIA MVKAFESIWK IKEEYNVTMR EAAYMHSIKK VAEAMKLRGW Y

References

[1]	"Identification of the latex test-reactive protein of Clostridium difficile as glutamate dehydrogenase." Lyerly D.M., Barroso L.A., Wilkins T.D. J. Clin. Microbiol. 29:2639-2642(1991) [PubMed: 1774279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M65250 Genomic DNA. Translation: AAA62756.1.
PIR	S28829.
3D structure databases
SMR	P27346. Positions 8-419.
ModBase	Search...
Phylogenomic databases
PhylomeDB	P27346.
Enzyme and pathway databases
BRENDA	1.4.1.2. 20175.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE2_CLODI

Accession

Primary (citable) accession number: P27346

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 19, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)