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P27344

- DPB3_YEAST

UniProt

P27344 - DPB3_YEAST

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Protein

DNA polymerase epsilon subunit C

Gene

DPB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA-directed DNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA-dependent DNA replication Source: SGD
  2. error-prone translesion synthesis Source: SGD
  3. heterochromatin organization involved in chromatin silencing Source: SGD
  4. leading strand elongation Source: SGD
  5. replicative senescence Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-29198-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit C (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit C
Gene namesi
Name:DPB3
Ordered Locus Names:YBR278W
ORF Names:YBR2015
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR278w.
SGDiS000000482. DPB3.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. epsilon DNA polymerase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 201201DNA polymerase epsilon subunit CPRO_0000208350Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei186 – 1861Phosphoserine3 Publications
Modified residuei188 – 1881Phosphoserine1 Publication
Modified residuei189 – 1891Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27344.
PaxDbiP27344.
PeptideAtlasiP27344.

Expressioni

Gene expression databases

GenevestigatoriP27344.

Interactioni

Subunit structurei

DNA polymerase epsilon is a heterotetramer consisting of POL2, DPB2, DPB3 and DPB4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DPB4Q046033EBI-6076,EBI-29938

Protein-protein interaction databases

BioGridi32972. 179 interactions.
DIPiDIP-4803N.
IntActiP27344. 13 interactions.
MINTiMINT-482796.
STRINGi4932.YBR278W.

Structurei

3D structure databases

ProteinModelPortaliP27344.
SMRiP27344. Positions 12-93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi120 – 15435Asp/Glu-rich (acidic)Add
BLAST

Phylogenomic databases

eggNOGiNOG309481.
InParanoidiP27344.
KOiK03506.
OMAiSMEEDIP.
OrthoDBiEOG7T1RQJ.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

P27344-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNLVKEKAP VFPISKVKKI AKCDPEYVIT SNVAISATAF AAELFVQNLV
60 70 80 90 100
EESLVLAQLN SKGKTSLRLS LNSIEECVEK RDNFRFLEDA IKQLKKNSAL
110 120 130 140 150
DKKRELNMQP GRSDQEVVIE EPELHEDDGV EEEEEEDEVS EEEEPVHNEE
160 170 180 190 200
LLDDSKDQQN DKSTRSVASL LSRFQYKSAL DVGEHSDSSD IEVDHTKSTD

P
Length:201
Mass (Da):22,665
Last modified:October 1, 1994 - v2
Checksum:i288913917D49C7F9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti196 – 1961T → M in CAA41403. (PubMed:1923754)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58500 Genomic DNA. Translation: CAA41403.1.
X76053 Genomic DNA. Translation: CAA53641.1.
Z36146 Genomic DNA. Translation: CAA85242.1.
BK006936 Genomic DNA. Translation: DAA07393.1.
PIRiS44540.
RefSeqiNP_009837.1. NM_001178626.1.

Genome annotation databases

EnsemblFungiiYBR278W; YBR278W; YBR278W.
GeneIDi852580.
KEGGisce:YBR278W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58500 Genomic DNA. Translation: CAA41403.1 .
X76053 Genomic DNA. Translation: CAA53641.1 .
Z36146 Genomic DNA. Translation: CAA85242.1 .
BK006936 Genomic DNA. Translation: DAA07393.1 .
PIRi S44540.
RefSeqi NP_009837.1. NM_001178626.1.

3D structure databases

ProteinModelPortali P27344.
SMRi P27344. Positions 12-93.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32972. 179 interactions.
DIPi DIP-4803N.
IntActi P27344. 13 interactions.
MINTi MINT-482796.
STRINGi 4932.YBR278W.

Proteomic databases

MaxQBi P27344.
PaxDbi P27344.
PeptideAtlasi P27344.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR278W ; YBR278W ; YBR278W .
GeneIDi 852580.
KEGGi sce:YBR278W.

Organism-specific databases

CYGDi YBR278w.
SGDi S000000482. DPB3.

Phylogenomic databases

eggNOGi NOG309481.
InParanoidi P27344.
KOi K03506.
OMAi SMEEDIP.
OrthoDBi EOG7T1RQJ.

Enzyme and pathway databases

BioCyci YEAST:G3O-29198-MONOMER.

Miscellaneous databases

NextBioi 971724.

Gene expression databases

Genevestigatori P27344.

Family and domain databases

Gene3Di 1.10.20.10. 1 hit.
InterProi IPR009072. Histone-fold.
[Graphical view ]
SUPFAMi SSF47113. SSF47113. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning DPB3, the gene encoding the third subunit of DNA polymerase II of Saccharomyces cerevisiae."
    Araki H., Hamatake R.K., Morrison A., Johnson A.L., Johnston L.H., Sugino A.
    Nucleic Acids Res. 19:4867-4872(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204626 / S288c / A364A.
  2. "The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
    Holmstroem K., Brandt T., Kallesoe T.
    Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Structure and function of the fourth subunit (Dpb4p) of DNA polymerase epsilon in Saccharomyces cerevisiae."
    Ohya T., Maki S., Kawasaki Y., Sugino A.
    Nucleic Acids Res. 28:3846-3852(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, SUBCELLULAR LOCATION.
  6. "Fidelity of DNA polymerase epsilon holoenzyme from budding yeast Saccharomyces cerevisiae."
    Shimizu K., Hashimoto K., Kirchner J.M., Nakai W., Nishikawa H., Resnick M.A., Sugino A.
    J. Biol. Chem. 277:37422-37429(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The quaternary structure of DNA polymerase epsilon from Saccharomyces cerevisiae."
    Chilkova O., Jonsson B.-H., Johansson E.
    J. Biol. Chem. 278:14082-14086(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF THE DNA POLYMERASE EPSILON COMPLEX.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186; SER-188 AND SER-189, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDPB3_YEAST
AccessioniPrimary (citable) accession number: P27344
Secondary accession number(s): D6VQS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Present with 784 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3