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Protein

DNA polymerase epsilon subunit C

Gene

DPB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

  • DNA-dependent DNA replication Source: SGD
  • error-prone translesion synthesis Source: SGD
  • heterochromatin organization involved in chromatin silencing Source: SGD
  • leading strand elongation Source: SGD
  • replicative senescence Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-29198-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit C (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit C
Gene namesi
Name:DPB3
Ordered Locus Names:YBR278W
ORF Names:YBR2015
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR278W.
SGDiS000000482. DPB3.

Subcellular locationi

GO - Cellular componenti

  • epsilon DNA polymerase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002083501 – 201DNA polymerase epsilon subunit CAdd BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186PhosphoserineCombined sources1
Modified residuei188PhosphoserineCombined sources1
Modified residuei189PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27344.
PRIDEiP27344.

PTM databases

iPTMnetiP27344.

Interactioni

Subunit structurei

DNA polymerase epsilon is a heterotetramer consisting of POL2, DPB2, DPB3 and DPB4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DPB4Q046033EBI-6076,EBI-29938

Protein-protein interaction databases

BioGridi32972. 179 interactors.
DIPiDIP-4803N.
IntActiP27344. 13 interactors.
MINTiMINT-482796.

Structurei

3D structure databases

ProteinModelPortaliP27344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi120 – 154Asp/Glu-rich (acidic)Add BLAST35

Phylogenomic databases

InParanoidiP27344.
KOiK03506.
OMAiDYIFLED.
OrthoDBiEOG092C51B1.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

P27344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLVKEKAP VFPISKVKKI AKCDPEYVIT SNVAISATAF AAELFVQNLV
60 70 80 90 100
EESLVLAQLN SKGKTSLRLS LNSIEECVEK RDNFRFLEDA IKQLKKNSAL
110 120 130 140 150
DKKRELNMQP GRSDQEVVIE EPELHEDDGV EEEEEEDEVS EEEEPVHNEE
160 170 180 190 200
LLDDSKDQQN DKSTRSVASL LSRFQYKSAL DVGEHSDSSD IEVDHTKSTD

P
Length:201
Mass (Da):22,665
Last modified:October 1, 1994 - v2
Checksum:i288913917D49C7F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti196T → M in CAA41403 (PubMed:1923754).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58500 Genomic DNA. Translation: CAA41403.1.
X76053 Genomic DNA. Translation: CAA53641.1.
Z36146 Genomic DNA. Translation: CAA85242.1.
BK006936 Genomic DNA. Translation: DAA07393.1.
PIRiS44540.
RefSeqiNP_009837.1. NM_001178626.1.

Genome annotation databases

EnsemblFungiiYBR278W; YBR278W; YBR278W.
GeneIDi852580.
KEGGisce:YBR278W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58500 Genomic DNA. Translation: CAA41403.1.
X76053 Genomic DNA. Translation: CAA53641.1.
Z36146 Genomic DNA. Translation: CAA85242.1.
BK006936 Genomic DNA. Translation: DAA07393.1.
PIRiS44540.
RefSeqiNP_009837.1. NM_001178626.1.

3D structure databases

ProteinModelPortaliP27344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32972. 179 interactors.
DIPiDIP-4803N.
IntActiP27344. 13 interactors.
MINTiMINT-482796.

PTM databases

iPTMnetiP27344.

Proteomic databases

MaxQBiP27344.
PRIDEiP27344.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR278W; YBR278W; YBR278W.
GeneIDi852580.
KEGGisce:YBR278W.

Organism-specific databases

EuPathDBiFungiDB:YBR278W.
SGDiS000000482. DPB3.

Phylogenomic databases

InParanoidiP27344.
KOiK03506.
OMAiDYIFLED.
OrthoDBiEOG092C51B1.

Enzyme and pathway databases

BioCyciYEAST:G3O-29198-MONOMER.

Miscellaneous databases

PROiP27344.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPB3_YEAST
AccessioniPrimary (citable) accession number: P27344
Secondary accession number(s): D6VQS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Present with 784 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.