##gff-version 3 P27321 UniProtKB Chain 1 713 . . . ID=PRO_0000147635;Note=Calpastatin P27321 UniProtKB Repeat 208 260 . . . Note=Inhibitory domain 1 P27321 UniProtKB Repeat 341 393 . . . Note=Inhibitory domain 2 P27321 UniProtKB Repeat 451 504 . . . Note=Inhibitory domain 3 P27321 UniProtKB Repeat 588 641 . . . Note=Inhibitory domain 4 P27321 UniProtKB Region 1 152 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P27321 UniProtKB Region 253 402 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P27321 UniProtKB Region 442 507 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P27321 UniProtKB Region 544 713 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P27321 UniProtKB Compositional bias 47 77 . . . Note=Polar residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P27321 UniProtKB Compositional bias 78 100 . . . Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P27321 UniProtKB Compositional bias 107 136 . . . Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P27321 UniProtKB Compositional bias 327 346 . . . Note=Polar residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P27321 UniProtKB Compositional bias 347 401 . . . Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P27321 UniProtKB Compositional bias 442 502 . . . Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P27321 UniProtKB Compositional bias 563 652 . . . Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P27321 UniProtKB Compositional bias 673 713 . . . Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P27321 UniProtKB Modified residue 57 57 . . . Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:22673903;Dbxref=PMID:22673903 P27321 UniProtKB Modified residue 86 86 . . . Note=N6-acetyllysine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P51125 P27321 UniProtKB Modified residue 122 122 . . . Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:22673903;Dbxref=PMID:22673903 P27321 UniProtKB Modified residue 171 171 . . . Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:22673903;Dbxref=PMID:22673903 P27321 UniProtKB Modified residue 173 173 . . . Note=Phosphothreonine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:22673903;Dbxref=PMID:22673903 P27321 UniProtKB Modified residue 260 260 . . . Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:22673903;Dbxref=PMID:22673903 P27321 UniProtKB Modified residue 281 281 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P20810 P27321 UniProtKB Modified residue 401 401 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P20810 P27321 UniProtKB Modified residue 403 403 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P20810 P27321 UniProtKB Modified residue 410 410 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P20810 P27321 UniProtKB Modified residue 445 445 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P20810 P27321 UniProtKB Modified residue 521 521 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P20810 P27321 UniProtKB Modified residue 532 532 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P20810 P27321 UniProtKB Modified residue 580 580 . . . Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:22673903;Dbxref=PMID:22673903 P27321 UniProtKB Modified residue 582 582 . . . Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:22673903;Dbxref=PMID:22673903 P27321 UniProtKB Cross-link 69 69 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P20810 P27321 UniProtKB Alternative sequence 1 177 . . . ID=VSP_026971;Note=In isoform 7. Missing;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:17570336;Dbxref=PMID:17570336 P27321 UniProtKB Alternative sequence 67 79 . . . ID=VSP_000753;Note=In isoform 4 and isoform 6. Missing;Ontology_term=ECO:0000303,ECO:0000303;evidence=ECO:0000303|PubMed:1730065,ECO:0000303|PubMed:17570336;Dbxref=PMID:1730065,PMID:17570336 P27321 UniProtKB Alternative sequence 98 135 . . . ID=VSP_000754;Note=In isoform 2%2C isoform 4%2C isoform 5 and isoform 6. Missing;Ontology_term=ECO:0000303,ECO:0000303,ECO:0000303,ECO:0000303,ECO:0000303;evidence=ECO:0000303|PubMed:15489334,ECO:0000303|PubMed:1730065,ECO:0000303|PubMed:17570336,ECO:0000303|PubMed:9475181,ECO:0000303|Ref.5;Dbxref=PMID:15489334,PMID:1730065,PMID:17570336,PMID:9475181 P27321 UniProtKB Alternative sequence 166 188 . . . ID=VSP_026972;Note=In isoform 3%2C isoform 5 and isoform 6. Missing;Ontology_term=ECO:0000303,ECO:0000303,ECO:0000303;evidence=ECO:0000303|PubMed:17570336,ECO:0000303|PubMed:9475181,ECO:0000303|Ref.5;Dbxref=PMID:17570336,PMID:9475181 P27321 UniProtKB Mutagenesis 613 613 . . . Note=2.9-fold increase in the IC(50). G->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19020623;Dbxref=PMID:19020623 P27321 UniProtKB Mutagenesis 613 613 . . . Note=Turns CAST from an inhibitor into a substrate. G->FG;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19020623;Dbxref=PMID:19020623 P27321 UniProtKB Sequence conflict 161 161 . . . Note=K->E;Ontology_term=ECO:0000305;evidence=ECO:0000305 P27321 UniProtKB Sequence conflict 209 209 . . . Note=N->S;Ontology_term=ECO:0000305;evidence=ECO:0000305 P27321 UniProtKB Sequence conflict 314 314 . . . Note=K->E;Ontology_term=ECO:0000305;evidence=ECO:0000305 P27321 UniProtKB Sequence conflict 463 463 . . . Note=V->G;Ontology_term=ECO:0000305;evidence=ECO:0000305 P27321 UniProtKB Sequence conflict 481 481 . . . Note=T->A;Ontology_term=ECO:0000305;evidence=ECO:0000305 P27321 UniProtKB Sequence conflict 518 518 . . . Note=D->G;Ontology_term=ECO:0000305;evidence=ECO:0000305 P27321 UniProtKB Sequence conflict 611 611 . . . Note=K->R;Ontology_term=ECO:0000305;evidence=ECO:0000305 P27321 UniProtKB Sequence conflict 646 646 . . . Note=L->P;Ontology_term=ECO:0000305;evidence=ECO:0000305 P27321 UniProtKB Helix 195 199 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3DF0 P27321 UniProtKB Helix 236 238 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3DF0 P27321 UniProtKB Helix 241 247 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3DF0 P27321 UniProtKB Helix 271 274 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3DF0 P27321 UniProtKB Turn 275 277 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3DF0 P27321 UniProtKB Helix 573 581 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3BOW P27321 UniProtKB Helix 616 618 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3BOW P27321 UniProtKB Helix 621 627 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3BOW P27321 UniProtKB Helix 652 659 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3BOW