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Protein

Ferredoxin-1

Gene

petF

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.

Cofactori

[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster.

Redox potential

E0 is -412 mV.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1 Publication
Metal bindingi45 – 451Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1 Publication
Metal bindingi48 – 481Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1 Publication
Metal bindingi78 – 781Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1 Publication

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: UniProtKB
  • electron carrier activity Source: InterPro
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ferredoxin-1
Alternative name(s):
Ferredoxin I
Gene namesi
Name:petF
Synonyms:fed
Ordered Locus Names:ssl0020
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 9796Ferredoxin-1PRO_0000189381Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi19 ↔ 86Curated

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Forms a complex with heterodimeric ferredoxin-thioredoxin reductase (FTR) and thioredoxin.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ftrCQ553892EBI-863421,EBI-863211
ftrVQ557812EBI-863421,EBI-863219
psaDP195693EBI-863421,EBI-595298

Protein-protein interaction databases

DIPiDIP-35027N.
IntActiP27320. 6 interactions.
MINTiMINT-3974388.

Structurei

Secondary structure

1
97
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 108Combined sources
Beta strandi13 – 208Combined sources
Helixi25 – 328Combined sources
Beta strandi39 – 468Combined sources
Beta strandi49 – 557Combined sources
Beta strandi63 – 664Combined sources
Helixi67 – 715Combined sources
Beta strandi74 – 763Combined sources
Helixi77 – 793Combined sources
Beta strandi81 – 899Combined sources
Helixi93 – 953Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DOXNMR-A2-97[»]
1DOYNMR-A2-97[»]
1OFFX-ray1.80A2-97[»]
2KAJNMR-A2-97[»]
2PVGX-ray2.40C2-97[»]
2PVOX-ray3.40D2-97[»]
5AUKX-ray1.62A2-97[»]
ProteinModelPortaliP27320.
SMRiP27320. Positions 2-97.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27320.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 94912Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the 2Fe2S plant-type ferredoxin family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000217152.
InParanoidiP27320.
KOiK02639.
OMAiCTIITHQ.
OrthoDBiEOG6GJC0W.
PhylomeDBiP27320.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR012675. Beta-grasp_dom.
IPR010241. Fd_pln.
[Graphical view]
PfamiPF00111. Fer2. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR02008. fdx_plant. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27320-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASYTVKLIT PDGESSIECS DDTYILDAAE EAGLDLPYSC RAGACSTCAG
60 70 80 90
KITAGSVDQS DQSFLDDDQI EAGYVLTCVA YPTSDCTIET HKEEDLY
Length:97
Mass (Da):10,363
Last modified:January 23, 2007 - v2
Checksum:i137FFD4F87A66DA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85607 Genomic DNA. Translation: BAA24020.1.
U38802 Genomic DNA. Translation: AAB72025.1.
BA000022 Genomic DNA. Translation: BAA10197.1.
PIRiS76345. FEYB6.

Genome annotation databases

EnsemblBacteriaiBAA10197; BAA10197; BAA10197.
KEGGisyn:ssl0020.
PATRICi23841858. VBISynSp132158_2473.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85607 Genomic DNA. Translation: BAA24020.1.
U38802 Genomic DNA. Translation: AAB72025.1.
BA000022 Genomic DNA. Translation: BAA10197.1.
PIRiS76345. FEYB6.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DOXNMR-A2-97[»]
1DOYNMR-A2-97[»]
1OFFX-ray1.80A2-97[»]
2KAJNMR-A2-97[»]
2PVGX-ray2.40C2-97[»]
2PVOX-ray3.40D2-97[»]
5AUKX-ray1.62A2-97[»]
ProteinModelPortaliP27320.
SMRiP27320. Positions 2-97.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-35027N.
IntActiP27320. 6 interactions.
MINTiMINT-3974388.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10197; BAA10197; BAA10197.
KEGGisyn:ssl0020.
PATRICi23841858. VBISynSp132158_2473.

Phylogenomic databases

HOGENOMiHOG000217152.
InParanoidiP27320.
KOiK02639.
OMAiCTIITHQ.
OrthoDBiEOG6GJC0W.
PhylomeDBiP27320.

Miscellaneous databases

EvolutionaryTraceiP27320.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR012675. Beta-grasp_dom.
IPR010241. Fd_pln.
[Graphical view]
PfamiPF00111. Fer2. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR02008. fdx_plant. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, characterization and transcriptional studies of ferredoxin genes from the mesophilic cyanobacterium Synechocystis sp. PCC 6803 and the thermophilic cyanobacterium Synechococcus vulcanus."
    Nakamoto H., Suzuki T.
    Physiol. Plantarum 101:199-205(1997)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Three insertion sequences from the cyanobacterium Synechocystis PCC6803 support the occurrence of horizontal DNA transfer among bacteria."
    Cassier-Chauvat C., Poncelet M., Chauvat F.
    Gene 195:257-266(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
    Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
    DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27184 / PCC 6803 / N-1.
  4. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  5. "Ferredoxin and flavodoxin from the cyanobacterium Synechocystis sp PCC 6803."
    Bottin H., Lagoutte B.
    Biochim. Biophys. Acta 1101:48-56(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-97.
  6. "1H and 15N NMR sequential assignment, secondary structure, and tertiary fold of [2Fe-2S] ferredoxin from Synechocystis sp. PCC 6803."
    Lelong C., Setif P., Bottin H., Andre F., Neumann J.-M.
    Biochemistry 34:14462-14473(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  7. "Structural snapshots along the reaction pathway of ferredoxin-thioredoxin reductase."
    Dai S., Friemann R., Glauser D.A., Bourquin F., Manieri W., Schurmann P., Eklund H.
    Nature 448:92-96(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 2-97 IN COMPLEXES WITH FTRC; FTRV AND TRX-F, SUBUNIT.

Entry informationi

Entry nameiFER_SYNY3
AccessioniPrimary (citable) accession number: P27320
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: March 16, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.