P27316 (L_RVFVZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L
EC=2.7.7.48

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Gene names

Name:

L

Organism

Rift valley fever virus (strain ZH-548 M12) (RVFV) [Reference proteome]

Taxonomic identifier

11589 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Bunyaviridae › Phlebovirus › Rift valley fever virus

Virus host

Aedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Bos taurus x Bison bison (beefalo) [TaxID: 297284]
Camelus bactrianus (Bactrian camel) [TaxID: 9837]
Capra hircus (Goat) [TaxID: 9925]
Homo sapiens (Human) [TaxID: 9606]
Ovis aries (Sheep) [TaxID: 9940]
Phlebotomus papatasi (Sandfly) [TaxID: 29031]

Protein attributes

Sequence length	2149 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Belongs to the phlebovirus RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleoside binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2149	2149	RNA-directed RNA polymerase L		PRO_0000222023
Regions
	Domain	975 – 1166	192	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P27316 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 8D5739C6079A88D7	FASTA	2,149	243,591
	10 20 30 40 50 60 MDSILSKQLV DKTGFVRVPI KHFDCTMLTL ALPTFDVSKM VDRITIDFNL DDIQGASEIG 70 80 90 100 110 120 STLLPSMSID VEDMANFVHD FTFGHLADKT DRLLMREFPM MNDGFDHLSP DMIIKTTSGV 130 140 150 160 170 180 YNIVEFTNFR GDERGAFQAA MIKLAKYEVP CENRSQGRTV VLYVVSAYRA WCMVYLELER 190 200 210 220 230 240 TLKQREMVYR YRLALSVMDE LRTLFPELSS TDEELGKTER ELPAMVSSIQ INWSVTESVF 250 260 270 280 290 300 PPFSREMFDR FRSSPPDSEY ITRIVSRCLI NSQEKLINSS FFAEGNDKAL RFSKNAEECS 310 320 330 340 350 360 LAVERALNQY RAEDNLRDLN DHKSTIQLPP WLSYHDVDGK DLCPLQGLDV RGDHPMCNLW 370 380 390 400 410 420 REVVTSANLE EIERMHDDAA AELEFAFGSK GQARERNRYH RVHLNMGSDV LVYIAALGVN 430 440 450 460 470 480 GKKHKADTLV QQMRDRSKQP FSPDHDVITY LNFSLHALVT CGQQMRTCTA LSLVIRDSVG 490 500 510 520 530 540 SPEDSSAILV RKGFHEIITE HYKFMGSRIG HGCQMVSLIG AELSASVKQH VKPNYFVIKR 550 560 570 580 590 600 LLGSGIFLLI KPTSSKSHIF VSFALSALAG PLISPLPGFS SPTKMLGILL VTDFVSYKLS 610 620 630 640 650 660 KLTNLCKCVS LMESSFSFWA EAFGIQAGTL VGDFVPRSSD SAAMDASYMG KLSLLTLLED 670 680 690 700 710 720 KAATEELQTI ARYIIMEGFV SPPEIPKPHK MTSKFPKVLR SELQVYLLNC LCRTIQRIAG 730 740 750 760 770 780 EPFILKKKDG SISWGGMFNP FSGRPLLDMQ PLISCCYNGY FKNKEEETEP SSLSGMYKKI 790 800 810 820 830 840 IELEHLRPQS DAFLGYKDPE LPRMHEFSVS YLKEACNHAK LVLRSLYGQN FMEQIDNQII 850 860 870 880 890 900 RELSGLTLER LATLKATSNF NENWYVYKDV ADKNYTRDKL LVKMSKYASE GKSLAIQKFE 910 920 930 940 950 960 DCMRQIESQG CMHICLFKKQ QHGGLREIYV MGAEERIVQS VVETIARSIG KFFASDTLCN 970 980 990 1000 1010 1020 PPNKVKIPET HGIRARKQCK GPVWTCATSD DARKWNQGHF VTKFALMLCE FTSPKWWPLI 1030 1040 1050 1060 1070 1080 IRGCSMFTKK RMMMNLNYLK ILDGHRELDI RDDFVMDLFK AYHGEAEVPW AFKGKTYLET 1090 1100 1110 1120 1130 1140 TTGMMQGILH YTSSLLHTIH QEYIRSLSFK IFNLKVAPEM SKSLVCDMMQ GSDDSSMLIS 1150 1160 1170 1180 1190 1200 FPADDEKVLT RCKVAAAICF RMKKELGVYL AIYPSEKSTA NTDFVMEYNS EFYFHTQHVR 1210 1220 1230 1240 1250 1260 PTIRWIAACC SLPEVETLVA RQEEASNLMT SVTEGGGSFS LAAIIQQAQC TLHYMLMGMG 1270 1280 1290 1300 1310 1320 VSELFLEYKK AVLKWNDPGL GFFLLDNPYA CGLGGFRFNL FKAITRTDLQ KLYAFFMKKV 1330 1340 1350 1360 1370 1380 KGSAARDWAD EDVTIPETCS VSPGGALILS SSLKWGSRKK FQKLRDRLNI PENWIELINE 1390 1400 1410 1420 1430 1440 NPEVLYRAPR TGPEILLRIA EKVHSPGVVS SLSSGNAVCK VMASAVYFLS ATIFEDTGRP 1450 1460 1470 1480 1490 1500 EFNFLEDSKY SLLQKMAAYS GFHGFNDMEP EDILFLFPNI EELESLDSIV YNKGEIDIIP 1510 1520 1530 1540 1550 1560 RVNIRDATQT RVTIFNEQKN LRTSPEKLVS DKWFGTQKSR IGKTTFLAEW EKLKKIVKWL 1570 1580 1590 1600 1610 1620 EDAPEATLAH TPLNNHIQVR NFFARMESKP RTVRITGAPV KKRSGVSKIA MVIRDHFSRM 1630 1640 1650 1660 1670 1680 GHLRGVEDLA GFTRSVSAEI LKHFLFCILQ GPYSESYKLQ LIYRVLSSVS NVEIKESDGK 1690 1700 1710 1720 1730 1740 TKTNLIGILQ RFLDGDHVVP IIEEMGAGTV GGFIKRQQSK VVQNKVVYYG VGIWRGFMDG 1750 1760 1770 1780 1790 1800 YQVHLEIEND IGQPPRLRNV TTNCQSSPWD LSIPIRQWAE DMGVTNNQDY SSKSSRGARY 1810 1820 1830 1840 1850 1860 WMHSFRMQGP SKPFGCPVYI IKGDMSDVIR LRKEEVEMKV RGSTLNLYTK HHSHQDLHIL 1870 1880 1890 1900 1910 1920 SYTASDNDLS PGIFKSISDE GVAQALQLFE REPSNCWVRC ESVAPKFISA ILEICEGKRQ 1930 1940 1950 1960 1970 1980 IKGINRTRLS EIVRICSESS LRSKVGSMFS FVANVEEAHD VDYDALMDLM IEDAKNNAFS 1990 2000 2010 2020 2030 2040 HVVDCIELDV SGPYEMESFH GRSTLTCTPS TILIRTYTFY LTLHQTMISV QAFQVILDEG 2050 2060 2070 2080 2090 2100 VLLIALVNNY LRGSKANCWV RCESVAPKFI SAILEICEGK RQIKGINRTR LSEIVEFVLN 2110 2120 2130 2140 LPKIKSRIYV LICRQCHGAN FPPISVRRLM LEDIASVARR LIIVASFGS

References

[1]

"Completion of the genome sequence of Rift Valley fever phlebovirus indicates that the L RNA is negative sense and codes for a putative transcriptase-replicase." Mueller R., Argentini C., Bouloy M., Prehaud C., Bishop D.H.L. Nucleic Acids Res. 19:5433-5433(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56464 Genomic RNA. Translation: CAA39836.1.
PIR	S18676.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR022531. DUF3770. IPR007099. RNA-dir_pol_NSvirus. IPR014385. RNA-dir_pol_phlebovirus. IPR007322. RNA_pol_bunyavir. [Graphical view]
Pfam	PF04196. Bunya_RdRp. 1 hit. PF12603. DUF3770. 1 hit. [Graphical view]
PIRSF	PIRSF000826. L_PhleboV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_RVFVZ

Accession

Primary (citable) accession number: P27316

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program