P27315 (GP_PHV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Envelope glycoprotein
Short name=GP

Alternative name(s):
M polyprotein

Cleaved into the following 2 chains:

1. Glycoprotein G1
2. Glycoprotein G2

Gene names

Name:

GP

Organism

Prospect Hill virus (PHV)

Taxonomic identifier

11603 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Bunyaviridae › Hantavirus

Virus host

Microtus pennsylvanicus (Meadow vole) [TaxID: 10058]

Protein attributes

Sequence length	1142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion. G1 contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis By similarity.
Subunit structure	G1 and G2 interacts with each other By similarity.
Subcellular location	Glycoprotein G1: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Interaction between G1 and G2 is essential for proper targeting of G1 to the Golgi complex, where virion budding occurs By similarity. Glycoprotein G2: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.
Sequence similarities	Belongs to the hantavirus envelope glycoprotein family. Contains 1 ITAM domain.

Ontologies

Keywords
Biological process	Fusion of virus membrane with host endosomal membrane Fusion of virus membrane with host membrane Host-virus interaction Inhibition of host IFN-mediated response initiation by virus Inhibition of host TRAFs by virus Inhibition of host innate immune response by virus Viral attachment to host cell Viral immunoevasion Viral penetration into host cytoplasm Virus entry into host cell
Cellular component	Host Golgi apparatus Host endoplasmic reticulum Host membrane Membrane Virion
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	signal transduction Inferred from electronic annotation. Source: InterPro suppression by virus of host TRAF activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host innate immune response Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon production Inferred from electronic annotation. Source: UniProtKB-KW viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 1142	1121	Envelope glycoprotein		PRO_0000036818
	Chain	22 – 654	633	Glycoprotein G1 By similarity		PRO_0000036819
	Chain	655 – 1142	488	Glycoprotein G2 By similarity		PRO_0000036820
Regions
	Topological domain	22 – 489	468	Lumenal Potential
	Transmembrane	490 – 510	21	Helical; Potential
	Topological domain	511 – 654	144	Cytoplasmic Potential
	Topological domain	655 – 1110	456	Lumenal Potential
	Transmembrane	1111 – 1131	21	Helical; Potential
	Topological domain	1132 – 1142	11	Cytoplasmic Potential
	Domain	617 – 640	24	ITAM
Sites
	Site	654 – 655	2	Cleavage; by host signal peptidase By similarity
Amino acid modifications
	Glycosylation	139	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	353	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	405	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	933	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	P27315 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: CD3BAD69F078C8B9	FASTA	1,142	125,686
	10 20 30 40 50 60 MSKFCLCLSL LGVLLLQVCD TRSLLELKIE CPHTVGLGQG LVIGTVDLNP VPVESVSTLK 70 80 90 100 110 120 LESSCNFDVH TSSATQQAVT KWTWEKKADT AETAKAASTT FQSKSTELNL RGLCVIPTLV 130 140 150 160 170 180 LETANKLRKT VTCYDLSCNQ TACIPTVYLI APIHTCVTTK SCLLGLGTQR IQVTYEKTYC 190 200 210 220 230 240 VSGQLVEGTC FNPIHTMALS QPSHTYDIVT IPVRCFFIAK KTNDDTLKIE KQFETILEKS 250 260 270 280 290 300 GCTAANIKGY YVCFLGATSE PIFVPTMDDF RASQILSDMA ISPHGEDHDS ALSSVSTFRI 310 320 330 340 350 360 AGKLSGKAPS TESSDTVQGV AFSGHPLYTS LSVLASKEDP VYIWSPGIIP ERNHTVCDKK 370 380 390 400 410 420 TLPLTWTGYL PLPGGIEKTT QCTIFCTLAG PGADCEAYSD TGIFNISSPT CLINRVQRFR 430 440 450 460 470 480 GAEQQIKFVC QRVDLDIVVY CNGMKKVILT KTLVIGQCIY TFTSVFSLMP GIAHSLAVEL 490 500 510 520 530 540 CVPGIHGWST IALLATFCFG WLLIPIISLV SIKIMLLFAY MCSKYSNDSK FRLLIEKVKQ 550 560 570 580 590 600 EYQKTMGSMV CEVCQQECEM AKELESHKKS CPNGMCPYCM NPTESTESAL QAHFKVCKLT 610 620 630 640 650 660 TRFQENLRKS LNPYEPKRGC YRTLSVFRYR SRCFVGLVWC ILLVLELVIW AASADTVEIK 670 680 690 700 710 720 TGWTDTAHGA GVIPLKSDLE LDFSLPSSAT YIYRRDLQNP ANEQERIPFH FQLQRQVIHA 730 740 750 760 770 780 EIQNLGHWMD GTFNLKTSFH CYGACEKYAY PWQTAKCFLE KDYEFETGWG CNPGDCPGVG 790 800 810 820 830 840 TGCTACGVYL DKLRSVGKVF KVISLKFTRR VCIQLGSEQS CKTIDSNDCL MTTSVKVCMI 850 860 870 880 890 900 GTVSKFQPGD TLLFLGPLEE GGIIFKQWCT TTCHFGDPGD IMSTPQGMQC PEHTGAFRKK 910 920 930 940 950 960 CAFATMPTCE YDGNTLSGYQ RMLATRDSFQ SFNITEPHIT SNSLEWVDPD SSLKDHINLV 970 980 990 1000 1010 1020 VNRDVSFQDL SENPCQVGVA VSSIDGAWGS GVGFNLVCSV SLTECASFLT SIKACDAAMC 1030 1040 1050 1060 1070 1080 YGATTANLVR GQNTVHILGK GGHSGSKFMC CHSTECSSTG LTAAAPHLDR VTGYNVIDND 1090 1100 1110 1120 1130 1140 KVFDDGSPEC GVHCWFKKSG EWLMGILSGN WMVVAVLVVL LILSIFLFSL CCPRRVVHKK SS

References

[1]	"Molecular characterization of the Prospect Hill virus M RNA segment: a comparison with the M RNA segments of other hantaviruses." Parrington M.A., Lee P.W., Kang C.Y. J. Gen. Virol. 72:1845-1854(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55129 mRNA. Translation: CAA38922.1.
PIR	GNVUPH. JQ1380.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016402. Envelope_gylcoprot_Hantavirus. IPR002534. Hanta_G1. IPR002532. Hanta_G2. IPR012316. ITAM_motif_hantavir-typ. [Graphical view]
Pfam	PF01567. Hanta_G1. 1 hit. PF01561. Hanta_G2. 1 hit. PF10538. ITAM_Cys-rich. 1 hit. [Graphical view]
PIRSF	PIRSF003945. M_poly_HantaV. 1 hit.
ProDom	PD001813. Hanta_G2. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51055. ITAM_1. False negative. PS51056. ITAM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GP_PHV

Accession

Primary (citable) accession number: P27315

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program