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Protein

Envelopment polyprotein

Gene

GP

Organism
Puumala virus (strain Sotkamo/V-2969/81)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Glycoprotein N and Glycoprotein C interact with each other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promote fusion of viral membrane with host endosomal membrane after endocytosis of the virion. Glycoprotein N contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host RLR pathway by virus, Inhibition of host TRAFs by virus, Viral attachment to host cell, Viral immunoevasion, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelopment polyprotein
Alternative name(s):
M polyprotein
Cleaved into the following 2 chains:
Glycoprotein NBy similarity
Short name:
Gn
Alternative name(s):
Glycoprotein G1
Glycoprotein CBy similarity
Short name:
Gc
Alternative name(s):
Glycoprotein G2
Gene namesi
Name:GP
OrganismiPuumala virus (strain Sotkamo/V-2969/81)
Taxonomic identifieri39002 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesBunyaviridaeHantavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]
Proteomesi
  • UP000008482 Componenti: Genome

Subcellular locationi

Glycoprotein N :
  • Virion membrane By similarity
  • Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
  • Host endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity

  • Note: Interaction between Glycoprotein N and Glycoprotein C is essential for proper targeting of Glycoprotein N to the Golgi complex, where virion budding occurs.By similarity
Glycoprotein C :
  • Virion membrane By similarity
  • Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 496LumenalSequence analysisAdd BLAST473
Transmembranei497 – 517HelicalSequence analysisAdd BLAST21
Topological domaini518 – 658CytoplasmicSequence analysisAdd BLAST141
Topological domaini659 – 1115LumenalSequence analysisAdd BLAST457
Transmembranei1116 – 1136HelicalSequence analysisAdd BLAST21
Topological domaini1137 – 1148CytoplasmicSequence analysisAdd BLAST12

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000003683124 – 1148Envelopment polyproteinAdd BLAST1125
ChainiPRO_000003683224 – 658Glycoprotein NBy similarityAdd BLAST635
ChainiPRO_0000036833659 – 1148Glycoprotein CBy similarityAdd BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi142N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi357N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi409N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi898N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi937N-linked (GlcNAc...); by hostSequence analysis1

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including Glycoprotein N and Glycoprotein C.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei658 – 659Cleavage; by host signal peptidaseBy similarity2

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Glycoprotein N and Glycoprotein C interacts with each other.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini621 – 644ITAMPROSITE-ProRule annotationAdd BLAST24

Sequence similaritiesi

Contains 1 ITAM domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR016402. Envelope_glycoprot_Hantavirus.
IPR002534. Hanta_G1.
IPR002532. Hanta_G2.
IPR012316. ITAM_motif_hantavir-typ.
[Graphical view]
PfamiPF01567. Hanta_G1. 1 hit.
PF01561. Hanta_G2. 1 hit.
PF10538. ITAM_Cys-rich. 1 hit.
[Graphical view]
PIRSFiPIRSF003945. M_poly_HantaV. 1 hit.
ProDomiPD001813. Hanta_G2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS51056. ITAM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27312-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSSPVCLY LILQGLLLFD TVNAKNLNEL KMECPHTIGL GQGLVVGSVE
60 70 80 90 100
LPPVPIQQIE SLKLESSCNF DLHTSTAGQQ SFTKWTWETK GDLAENTQAS
110 120 130 140 150
STSFQTKSSE VNLRGLCLIP TLVVETAARM RKTIACYDLS CNQTVCQPTV
160 170 180 190 200
YLMGPIQTCL TTKSCLLGLG DQRIQVNYER TYCVSGQLVE GVCFNPIHTM
210 220 230 240 250
ALSQPSHTYD IVTIMVRCFL VIKKVTSGDS MKIEKNFETL VQKTGCTANG
260 270 280 290 300
FQGYYICLIG SSSEPLYVPT LDDYRSAEVL SRMAFAPHGE DHDIEKNAVS
310 320 330 340 350
ALRIAGKVTG KAPSTESSDT VQGIAFSGSP LYTSTGVLTA KDDPVYVWAP
360 370 380 390 400
GIIMEGNHSV CEKKTLPLTW TGFIPLPGEI EKTTQCTVFC TLAGPGADCE
410 420 430 440 450
AYSETGIFNI SSPTCLINRV QRFRGAEQQI KFVCQRVDMD ITVYCNGVKK
460 470 480 490 500
VILTKTLVIG QCIYTFTSIF SMIPGIAHSL AVELCVPGLH GWATVLLLLT
510 520 530 540 550
FCFGWVLIPT ITMILLKILI AFAYLCSKYN TDSKFRILVE KVKKEYQKTM
560 570 580 590 600
GSMVCEVCQY ECETAKELES HRKSCSIGSC PYCLNPSEAT PSALQAHFKV
610 620 630 640 650
CKLTSRFQEN LKKSLTMYEP MQGCYRTLSL FRYRSRFFVG LVWCMLLVLE
660 670 680 690 700
LIVWAASAET QNLNDGWTDT AHGSGIIPMK ADLELDFSLP SSASYTYRRQ
710 720 730 740 750
LQNPANEQEK IPFHLQISKQ VIHAEIQHLG HWMDATFNLK TAFHCYGSCE
760 770 780 790 800
KYAYPWQTAG CFVEKDYEYE TGWGCNPPDC PGVGTGCTAC GVYLDKLKSV
810 820 830 840 850
GKVFKIVSLR YTRKVCIQLG TGQTCKTVDS NDCLITTSVK VCLIGTISKF
860 870 880 890 900
QPSDTLLFLG PLQQGGLIFK QWCTTTCQFG DPGDIMSTPT GMKCPELNGS
910 920 930 940 950
FRKKCAFATT PVCQFDGNTI SGYKRMVATK DSFQSFNVTE PHISTSALEW
960 970 980 990 1000
IDLDSSLRDH INVIVSRDLS FQDLSETPCQ VDLTTSATDG AWGSGVGFNL
1010 1020 1030 1040 1050
VCTVSLTECS AFLTSIKACH AAMCYGSTTT NLVRGQNTIH VVGKGGHSGS
1060 1070 1080 1090 1100
KFMCCHDTKC SSTGLVAAAP HLDRVTGFNQ ADSDKIFDDG APECGMSCWF
1110 1120 1130 1140
KKLGEWVLGV LNGNWMVVAV LIALLILSIF LFALCCPRRP SYKKDHKP
Length:1,148
Mass (Da):126,294
Last modified:August 1, 1992 - v1
Checksum:iBA606F74979EE73F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti25K → R in strain: isolate Sotkamo 2009/WHO Arbovirus collection. 1
Natural varianti472M → L in strain: isolate Sotkamo 2009/WHO Arbovirus collection. 1
Natural varianti536R → K in strain: isolate Sotkamo 2009/WHO Arbovirus collection. 1
Natural varianti665D → A in strain: isolate Sotkamo 2009/WHO Arbovirus collection. 1
Natural varianti822G → E in strain: isolate Sotkamo 2009/WHO Arbovirus collection. 1
Natural varianti953L → P in strain: isolate Sotkamo 2009/WHO Arbovirus collection. 1
Natural varianti988T → I in strain: isolate Sotkamo 2009/WHO Arbovirus collection. 1
Natural varianti1020H → D in strain: isolate Sotkamo 2009/WHO Arbovirus collection. 1
Natural varianti1145D → E in strain: isolate Sotkamo 2009/WHO Arbovirus collection. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61034 Genomic RNA. Translation: CAA43369.1.
HE801634 Genomic RNA. Translation: CCH22848.1.
PIRiJQ1604.
RefSeqiNP_941983.1. NC_005223.1.

Genome annotation databases

GeneIDi2943082.
KEGGivg:2943082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61034 Genomic RNA. Translation: CAA43369.1.
HE801634 Genomic RNA. Translation: CCH22848.1.
PIRiJQ1604.
RefSeqiNP_941983.1. NC_005223.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2943082.
KEGGivg:2943082.

Family and domain databases

InterProiIPR016402. Envelope_glycoprot_Hantavirus.
IPR002534. Hanta_G1.
IPR002532. Hanta_G2.
IPR012316. ITAM_motif_hantavir-typ.
[Graphical view]
PfamiPF01567. Hanta_G1. 1 hit.
PF01561. Hanta_G2. 1 hit.
PF10538. ITAM_Cys-rich. 1 hit.
[Graphical view]
PIRSFiPIRSF003945. M_poly_HantaV. 1 hit.
ProDomiPD001813. Hanta_G2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS51056. ITAM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGP_PUUMS
AccessioniPrimary (citable) accession number: P27312
Secondary accession number(s): I4EPA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.