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P27312

- GP_PUUMS

UniProt

P27312 - GP_PUUMS

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Protein

Envelope glycoprotein

Gene

GP

Organism
Puumala virus (strain Sotkamo/V-2969/81)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion. G1 contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei658 – 6592Cleavage; by host signal peptidaseBy similarity

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. signal transduction Source: InterPro
  3. suppression by virus of host innate immune response Source: UniProtKB-KW
  4. suppression by virus of host TRAF activity Source: UniProtKB-KW
  5. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host RLR pathway by virus, Inhibition of host TRAFs by virus, Viral attachment to host cell, Viral immunoevasion, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein
Short name:
GP
Alternative name(s):
M polyprotein
Cleaved into the following 2 chains:
Gene namesi
Name:GP
OrganismiPuumala virus (strain Sotkamo/V-2969/81)
Taxonomic identifieri39002 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeHantavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]
ProteomesiUP000008482: Genome

Subcellular locationi

Chain Glycoprotein G1 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated. Host endoplasmic reticulum membrane Curated; Single-pass type I membrane protein Curated
Note: Interaction between G1 and G2 is essential for proper targeting of G1 to the Golgi complex, where virion budding occurs.By similarity

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. host cell Golgi apparatus Source: UniProtKB-KW
  3. host cell membrane Source: UniProtKB-KW
  4. integral component of membrane Source: UniProtKB-KW
  5. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 11481125Envelope glycoproteinPRO_0000036831Add
BLAST
Chaini24 – 658635Glycoprotein G1By similarityPRO_0000036832Add
BLAST
Chaini659 – 1148490Glycoprotein G2By similarityPRO_0000036833Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi142 – 1421N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi357 – 3571N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi409 – 4091N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi898 – 8981N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi937 – 9371N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

G1 and G2 interacts with each other.By similarity

Structurei

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 496473LumenalSequence AnalysisAdd
BLAST
Topological domaini518 – 658141CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini659 – 1115457LumenalSequence AnalysisAdd
BLAST
Topological domaini1137 – 114812CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei497 – 51721HelicalSequence AnalysisAdd
BLAST
Transmembranei1116 – 113621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini621 – 64424ITAMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ITAM domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR016402. Envelope_gylcoprot_Hantavirus.
IPR002534. Hanta_G1.
IPR002532. Hanta_G2.
IPR012316. ITAM_motif_hantavir-typ.
[Graphical view]
PfamiPF01567. Hanta_G1. 1 hit.
PF01561. Hanta_G2. 1 hit.
PF10538. ITAM_Cys-rich. 1 hit.
[Graphical view]
PIRSFiPIRSF003945. M_poly_HantaV. 1 hit.
ProDomiPD001813. Hanta_G2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS51056. ITAM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27312 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MGKSSPVCLY LILQGLLLFD TVNAKNLNEL KMECPHTIGL GQGLVVGSVE
60 70 80 90 100
LPPVPIQQIE SLKLESSCNF DLHTSTAGQQ SFTKWTWETK GDLAENTQAS
110 120 130 140 150
STSFQTKSSE VNLRGLCLIP TLVVETAARM RKTIACYDLS CNQTVCQPTV
160 170 180 190 200
YLMGPIQTCL TTKSCLLGLG DQRIQVNYER TYCVSGQLVE GVCFNPIHTM
210 220 230 240 250
ALSQPSHTYD IVTIMVRCFL VIKKVTSGDS MKIEKNFETL VQKTGCTANG
260 270 280 290 300
FQGYYICLIG SSSEPLYVPT LDDYRSAEVL SRMAFAPHGE DHDIEKNAVS
310 320 330 340 350
ALRIAGKVTG KAPSTESSDT VQGIAFSGSP LYTSTGVLTA KDDPVYVWAP
360 370 380 390 400
GIIMEGNHSV CEKKTLPLTW TGFIPLPGEI EKTTQCTVFC TLAGPGADCE
410 420 430 440 450
AYSETGIFNI SSPTCLINRV QRFRGAEQQI KFVCQRVDMD ITVYCNGVKK
460 470 480 490 500
VILTKTLVIG QCIYTFTSIF SMIPGIAHSL AVELCVPGLH GWATVLLLLT
510 520 530 540 550
FCFGWVLIPT ITMILLKILI AFAYLCSKYN TDSKFRILVE KVKKEYQKTM
560 570 580 590 600
GSMVCEVCQY ECETAKELES HRKSCSIGSC PYCLNPSEAT PSALQAHFKV
610 620 630 640 650
CKLTSRFQEN LKKSLTMYEP MQGCYRTLSL FRYRSRFFVG LVWCMLLVLE
660 670 680 690 700
LIVWAASAET QNLNDGWTDT AHGSGIIPMK ADLELDFSLP SSASYTYRRQ
710 720 730 740 750
LQNPANEQEK IPFHLQISKQ VIHAEIQHLG HWMDATFNLK TAFHCYGSCE
760 770 780 790 800
KYAYPWQTAG CFVEKDYEYE TGWGCNPPDC PGVGTGCTAC GVYLDKLKSV
810 820 830 840 850
GKVFKIVSLR YTRKVCIQLG TGQTCKTVDS NDCLITTSVK VCLIGTISKF
860 870 880 890 900
QPSDTLLFLG PLQQGGLIFK QWCTTTCQFG DPGDIMSTPT GMKCPELNGS
910 920 930 940 950
FRKKCAFATT PVCQFDGNTI SGYKRMVATK DSFQSFNVTE PHISTSALEW
960 970 980 990 1000
IDLDSSLRDH INVIVSRDLS FQDLSETPCQ VDLTTSATDG AWGSGVGFNL
1010 1020 1030 1040 1050
VCTVSLTECS AFLTSIKACH AAMCYGSTTT NLVRGQNTIH VVGKGGHSGS
1060 1070 1080 1090 1100
KFMCCHDTKC SSTGLVAAAP HLDRVTGFNQ ADSDKIFDDG APECGMSCWF
1110 1120 1130 1140
KKLGEWVLGV LNGNWMVVAV LIALLILSIF LFALCCPRRP SYKKDHKP
Length:1,148
Mass (Da):126,294
Last modified:August 1, 1992 - v1
Checksum:iBA606F74979EE73F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti25 – 251K → R in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti472 – 4721M → L in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti536 – 5361R → K in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti665 – 6651D → A in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti822 – 8221G → E in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti953 – 9531L → P in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti988 – 9881T → I in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti1020 – 10201H → D in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti1145 – 11451D → E in strain: isolate Sotkamo 2009/WHO Arbovirus collection.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61034 Genomic RNA. Translation: CAA43369.1.
HE801634 Genomic RNA. Translation: CCH22848.1.
PIRiJQ1604.
RefSeqiNP_941983.1. NC_005223.1.

Genome annotation databases

GeneIDi2943082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61034 Genomic RNA. Translation: CAA43369.1 .
HE801634 Genomic RNA. Translation: CCH22848.1 .
PIRi JQ1604.
RefSeqi NP_941983.1. NC_005223.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2943082.

Family and domain databases

InterProi IPR016402. Envelope_gylcoprot_Hantavirus.
IPR002534. Hanta_G1.
IPR002532. Hanta_G2.
IPR012316. ITAM_motif_hantavir-typ.
[Graphical view ]
Pfami PF01567. Hanta_G1. 1 hit.
PF01561. Hanta_G2. 1 hit.
PF10538. ITAM_Cys-rich. 1 hit.
[Graphical view ]
PIRSFi PIRSF003945. M_poly_HantaV. 1 hit.
ProDomi PD001813. Hanta_G2. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
PROSITEi PS51056. ITAM_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of Puumala virus Sotkamo strain S and M RNA segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein."
    Vapalahti O.P., Kallio-Kokko H., Salonen E.M., Brummer-Korvenkontio M., Vaheri A.
    J. Gen. Virol. 73:829-838(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Resequencing of the Puumala virus strain Sotkamo from the WHO Arbovirus collection."
    Kurolt I.C., Paessler S., Markotic A.
    Virus Genes 45:389-392(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Sotkamo 2009/WHO Arbovirus collection.

Entry informationi

Entry nameiGP_PUUMS
AccessioniPrimary (citable) accession number: P27312
Secondary accession number(s): I4EPA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3