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P27312

- GP_PUUMS

UniProt

P27312 - GP_PUUMS

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Protein
Envelope glycoprotein
Gene
GP
Organism
Puumala virus (strain Sotkamo/V-2969/81)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion. G1 contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei658 – 6592Cleavage; by host signal peptidase By similarity

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. signal transduction Source: InterPro
  3. suppression by virus of host TRAF activity Source: UniProtKB-KW
  4. suppression by virus of host innate immune response Source: UniProtKB-KW
  5. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host RLR pathway by virus, Inhibition of host TRAFs by virus, Viral attachment to host cell, Viral immunoevasion, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein
Short name:
GP
Alternative name(s):
M polyprotein
Cleaved into the following 2 chains:
Gene namesi
Name:GP
OrganismiPuumala virus (strain Sotkamo/V-2969/81)
Taxonomic identifieri39002 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeHantavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]
ProteomesiUP000008482: Genome

Subcellular locationi

Chain Glycoprotein G1 : Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host Golgi apparatus membrane; Single-pass type I membrane protein Reviewed prediction. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Reviewed prediction
Note: Interaction between G1 and G2 is essential for proper targeting of G1 to the Golgi complex, where virion budding occurs By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 496473Lumenal Reviewed prediction
Add
BLAST
Transmembranei497 – 51721Helical; Reviewed prediction
Add
BLAST
Topological domaini518 – 658141Cytoplasmic Reviewed prediction
Add
BLAST
Topological domaini659 – 1115457Lumenal Reviewed prediction
Add
BLAST
Transmembranei1116 – 113621Helical; Reviewed prediction
Add
BLAST
Topological domaini1137 – 114812Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell Golgi membrane Source: UniProtKB-SubCell
  2. host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed prediction
Add
BLAST
Chaini24 – 11481125Envelope glycoprotein
PRO_0000036831Add
BLAST
Chaini24 – 658635Glycoprotein G1 By similarity
PRO_0000036832Add
BLAST
Chaini659 – 1148490Glycoprotein G2 By similarity
PRO_0000036833Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi142 – 1421N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi357 – 3571N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi409 – 4091N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi898 – 8981N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi937 – 9371N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

G1 and G2 interacts with each other By similarity.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini621 – 64424ITAM
Add
BLAST

Sequence similaritiesi

Contains 1 ITAM domain.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR016402. Envelope_gylcoprot_Hantavirus.
IPR002534. Hanta_G1.
IPR002532. Hanta_G2.
IPR012316. ITAM_motif_hantavir-typ.
[Graphical view]
PfamiPF01567. Hanta_G1. 1 hit.
PF01561. Hanta_G2. 1 hit.
PF10538. ITAM_Cys-rich. 1 hit.
[Graphical view]
PIRSFiPIRSF003945. M_poly_HantaV. 1 hit.
ProDomiPD001813. Hanta_G2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS51056. ITAM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27312-1 [UniParc]FASTAAdd to Basket

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MGKSSPVCLY LILQGLLLFD TVNAKNLNEL KMECPHTIGL GQGLVVGSVE     50
LPPVPIQQIE SLKLESSCNF DLHTSTAGQQ SFTKWTWETK GDLAENTQAS 100
STSFQTKSSE VNLRGLCLIP TLVVETAARM RKTIACYDLS CNQTVCQPTV 150
YLMGPIQTCL TTKSCLLGLG DQRIQVNYER TYCVSGQLVE GVCFNPIHTM 200
ALSQPSHTYD IVTIMVRCFL VIKKVTSGDS MKIEKNFETL VQKTGCTANG 250
FQGYYICLIG SSSEPLYVPT LDDYRSAEVL SRMAFAPHGE DHDIEKNAVS 300
ALRIAGKVTG KAPSTESSDT VQGIAFSGSP LYTSTGVLTA KDDPVYVWAP 350
GIIMEGNHSV CEKKTLPLTW TGFIPLPGEI EKTTQCTVFC TLAGPGADCE 400
AYSETGIFNI SSPTCLINRV QRFRGAEQQI KFVCQRVDMD ITVYCNGVKK 450
VILTKTLVIG QCIYTFTSIF SMIPGIAHSL AVELCVPGLH GWATVLLLLT 500
FCFGWVLIPT ITMILLKILI AFAYLCSKYN TDSKFRILVE KVKKEYQKTM 550
GSMVCEVCQY ECETAKELES HRKSCSIGSC PYCLNPSEAT PSALQAHFKV 600
CKLTSRFQEN LKKSLTMYEP MQGCYRTLSL FRYRSRFFVG LVWCMLLVLE 650
LIVWAASAET QNLNDGWTDT AHGSGIIPMK ADLELDFSLP SSASYTYRRQ 700
LQNPANEQEK IPFHLQISKQ VIHAEIQHLG HWMDATFNLK TAFHCYGSCE 750
KYAYPWQTAG CFVEKDYEYE TGWGCNPPDC PGVGTGCTAC GVYLDKLKSV 800
GKVFKIVSLR YTRKVCIQLG TGQTCKTVDS NDCLITTSVK VCLIGTISKF 850
QPSDTLLFLG PLQQGGLIFK QWCTTTCQFG DPGDIMSTPT GMKCPELNGS 900
FRKKCAFATT PVCQFDGNTI SGYKRMVATK DSFQSFNVTE PHISTSALEW 950
IDLDSSLRDH INVIVSRDLS FQDLSETPCQ VDLTTSATDG AWGSGVGFNL 1000
VCTVSLTECS AFLTSIKACH AAMCYGSTTT NLVRGQNTIH VVGKGGHSGS 1050
KFMCCHDTKC SSTGLVAAAP HLDRVTGFNQ ADSDKIFDDG APECGMSCWF 1100
KKLGEWVLGV LNGNWMVVAV LIALLILSIF LFALCCPRRP SYKKDHKP 1148
Length:1,148
Mass (Da):126,294
Last modified:August 1, 1992 - v1
Checksum:iBA606F74979EE73F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti25 – 251K → R in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti472 – 4721M → L in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti536 – 5361R → K in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti665 – 6651D → A in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti822 – 8221G → E in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti953 – 9531L → P in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti988 – 9881T → I in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti1020 – 10201H → D in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural varianti1145 – 11451D → E in strain: isolate Sotkamo 2009/WHO Arbovirus collection.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61034 Genomic RNA. Translation: CAA43369.1.
HE801634 Genomic RNA. Translation: CCH22848.1.
PIRiJQ1604.
RefSeqiNP_941983.1. NC_005223.1.

Genome annotation databases

GeneIDi2943082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61034 Genomic RNA. Translation: CAA43369.1 .
HE801634 Genomic RNA. Translation: CCH22848.1 .
PIRi JQ1604.
RefSeqi NP_941983.1. NC_005223.1.

3D structure databases

ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2943082.

Family and domain databases

InterProi IPR016402. Envelope_gylcoprot_Hantavirus.
IPR002534. Hanta_G1.
IPR002532. Hanta_G2.
IPR012316. ITAM_motif_hantavir-typ.
[Graphical view ]
Pfami PF01567. Hanta_G1. 1 hit.
PF01561. Hanta_G2. 1 hit.
PF10538. ITAM_Cys-rich. 1 hit.
[Graphical view ]
PIRSFi PIRSF003945. M_poly_HantaV. 1 hit.
ProDomi PD001813. Hanta_G2. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
PROSITEi PS51056. ITAM_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of Puumala virus Sotkamo strain S and M RNA segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein."
    Vapalahti O.P., Kallio-Kokko H., Salonen E.M., Brummer-Korvenkontio M., Vaheri A.
    J. Gen. Virol. 73:829-838(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Resequencing of the Puumala virus strain Sotkamo from the WHO Arbovirus collection."
    Kurolt I.C., Paessler S., Markotic A.
    Virus Genes 45:389-392(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Sotkamo 2009/WHO Arbovirus collection.

Entry informationi

Entry nameiGP_PUUMS
AccessioniPrimary (citable) accession number: P27312
Secondary accession number(s): I4EPA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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