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P27312

- GP_PUUMS

UniProt

P27312 - GP_PUUMS

Protein

Envelope glycoprotein

Gene

GP

Organism
Puumala virus (strain Sotkamo/V-2969/81)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion. G1 contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei658 – 6592Cleavage; by host signal peptidaseBy similarity

    GO - Biological processi

    1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
    2. signal transduction Source: InterPro
    3. suppression by virus of host innate immune response Source: UniProtKB-KW
    4. suppression by virus of host TRAF activity Source: UniProtKB-KW
    5. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host RLR pathway by virus, Inhibition of host TRAFs by virus, Viral attachment to host cell, Viral immunoevasion, Viral penetration into host cytoplasm, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Envelope glycoprotein
    Short name:
    GP
    Alternative name(s):
    M polyprotein
    Cleaved into the following 2 chains:
    Gene namesi
    Name:GP
    OrganismiPuumala virus (strain Sotkamo/V-2969/81)
    Taxonomic identifieri39002 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeHantavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]
    ProteomesiUP000008482: Genome

    Subcellular locationi

    Chain Glycoprotein G1 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated. Host endoplasmic reticulum membrane Curated; Single-pass type I membrane protein Curated
    Note: Interaction between G1 and G2 is essential for proper targeting of G1 to the Golgi complex, where virion budding occurs.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. host cell Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 11481125Envelope glycoproteinPRO_0000036831Add
    BLAST
    Chaini24 – 658635Glycoprotein G1By similarityPRO_0000036832Add
    BLAST
    Chaini659 – 1148490Glycoprotein G2By similarityPRO_0000036833Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi142 – 1421N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi357 – 3571N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi409 – 4091N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi898 – 8981N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi937 – 9371N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    G1 and G2 interacts with each other.By similarity

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 496473LumenalSequence AnalysisAdd
    BLAST
    Topological domaini518 – 658141CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini659 – 1115457LumenalSequence AnalysisAdd
    BLAST
    Topological domaini1137 – 114812CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei497 – 51721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1116 – 113621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini621 – 64424ITAMPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ITAM domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR016402. Envelope_gylcoprot_Hantavirus.
    IPR002534. Hanta_G1.
    IPR002532. Hanta_G2.
    IPR012316. ITAM_motif_hantavir-typ.
    [Graphical view]
    PfamiPF01567. Hanta_G1. 1 hit.
    PF01561. Hanta_G2. 1 hit.
    PF10538. ITAM_Cys-rich. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003945. M_poly_HantaV. 1 hit.
    ProDomiPD001813. Hanta_G2. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    PROSITEiPS51056. ITAM_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27312-1 [UniParc]FASTAAdd to Basket

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    MGKSSPVCLY LILQGLLLFD TVNAKNLNEL KMECPHTIGL GQGLVVGSVE     50
    LPPVPIQQIE SLKLESSCNF DLHTSTAGQQ SFTKWTWETK GDLAENTQAS 100
    STSFQTKSSE VNLRGLCLIP TLVVETAARM RKTIACYDLS CNQTVCQPTV 150
    YLMGPIQTCL TTKSCLLGLG DQRIQVNYER TYCVSGQLVE GVCFNPIHTM 200
    ALSQPSHTYD IVTIMVRCFL VIKKVTSGDS MKIEKNFETL VQKTGCTANG 250
    FQGYYICLIG SSSEPLYVPT LDDYRSAEVL SRMAFAPHGE DHDIEKNAVS 300
    ALRIAGKVTG KAPSTESSDT VQGIAFSGSP LYTSTGVLTA KDDPVYVWAP 350
    GIIMEGNHSV CEKKTLPLTW TGFIPLPGEI EKTTQCTVFC TLAGPGADCE 400
    AYSETGIFNI SSPTCLINRV QRFRGAEQQI KFVCQRVDMD ITVYCNGVKK 450
    VILTKTLVIG QCIYTFTSIF SMIPGIAHSL AVELCVPGLH GWATVLLLLT 500
    FCFGWVLIPT ITMILLKILI AFAYLCSKYN TDSKFRILVE KVKKEYQKTM 550
    GSMVCEVCQY ECETAKELES HRKSCSIGSC PYCLNPSEAT PSALQAHFKV 600
    CKLTSRFQEN LKKSLTMYEP MQGCYRTLSL FRYRSRFFVG LVWCMLLVLE 650
    LIVWAASAET QNLNDGWTDT AHGSGIIPMK ADLELDFSLP SSASYTYRRQ 700
    LQNPANEQEK IPFHLQISKQ VIHAEIQHLG HWMDATFNLK TAFHCYGSCE 750
    KYAYPWQTAG CFVEKDYEYE TGWGCNPPDC PGVGTGCTAC GVYLDKLKSV 800
    GKVFKIVSLR YTRKVCIQLG TGQTCKTVDS NDCLITTSVK VCLIGTISKF 850
    QPSDTLLFLG PLQQGGLIFK QWCTTTCQFG DPGDIMSTPT GMKCPELNGS 900
    FRKKCAFATT PVCQFDGNTI SGYKRMVATK DSFQSFNVTE PHISTSALEW 950
    IDLDSSLRDH INVIVSRDLS FQDLSETPCQ VDLTTSATDG AWGSGVGFNL 1000
    VCTVSLTECS AFLTSIKACH AAMCYGSTTT NLVRGQNTIH VVGKGGHSGS 1050
    KFMCCHDTKC SSTGLVAAAP HLDRVTGFNQ ADSDKIFDDG APECGMSCWF 1100
    KKLGEWVLGV LNGNWMVVAV LIALLILSIF LFALCCPRRP SYKKDHKP 1148
    Length:1,148
    Mass (Da):126,294
    Last modified:August 1, 1992 - v1
    Checksum:iBA606F74979EE73F
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti25 – 251K → R in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
    Natural varianti472 – 4721M → L in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
    Natural varianti536 – 5361R → K in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
    Natural varianti665 – 6651D → A in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
    Natural varianti822 – 8221G → E in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
    Natural varianti953 – 9531L → P in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
    Natural varianti988 – 9881T → I in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
    Natural varianti1020 – 10201H → D in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
    Natural varianti1145 – 11451D → E in strain: isolate Sotkamo 2009/WHO Arbovirus collection.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61034 Genomic RNA. Translation: CAA43369.1.
    HE801634 Genomic RNA. Translation: CCH22848.1.
    PIRiJQ1604.
    RefSeqiNP_941983.1. NC_005223.1.

    Genome annotation databases

    GeneIDi2943082.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61034 Genomic RNA. Translation: CAA43369.1 .
    HE801634 Genomic RNA. Translation: CCH22848.1 .
    PIRi JQ1604.
    RefSeqi NP_941983.1. NC_005223.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2943082.

    Family and domain databases

    InterProi IPR016402. Envelope_gylcoprot_Hantavirus.
    IPR002534. Hanta_G1.
    IPR002532. Hanta_G2.
    IPR012316. ITAM_motif_hantavir-typ.
    [Graphical view ]
    Pfami PF01567. Hanta_G1. 1 hit.
    PF01561. Hanta_G2. 1 hit.
    PF10538. ITAM_Cys-rich. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003945. M_poly_HantaV. 1 hit.
    ProDomi PD001813. Hanta_G2. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    PROSITEi PS51056. ITAM_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of Puumala virus Sotkamo strain S and M RNA segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein."
      Vapalahti O.P., Kallio-Kokko H., Salonen E.M., Brummer-Korvenkontio M., Vaheri A.
      J. Gen. Virol. 73:829-838(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Resequencing of the Puumala virus strain Sotkamo from the WHO Arbovirus collection."
      Kurolt I.C., Paessler S., Markotic A.
      Virus Genes 45:389-392(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Sotkamo 2009/WHO Arbovirus collection.

    Entry informationi

    Entry nameiGP_PUUMS
    AccessioniPrimary (citable) accession number: P27312
    Secondary accession number(s): I4EPA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3