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P27312 (GP_PUUMS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Envelope glycoprotein

Short name=GP
Alternative name(s):
M polyprotein

Cleaved into the following 2 chains:

  1. Glycoprotein G1
  2. Glycoprotein G2
Gene names
Name:GP
OrganismPuumala virus (strain Sotkamo/V-2969/81) [Complete proteome]
Taxonomic identifier39002 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesBunyaviridaeHantavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]
Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]

Protein attributes

Sequence length1148 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion. G1 contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis By similarity.

Subunit structure

G1 and G2 interacts with each other By similarity.

Subcellular location

Glycoprotein G1: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Interaction between G1 and G2 is essential for proper targeting of G1 to the Golgi complex, where virion budding occurs By similarity.

Glycoprotein G2: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential.

Post-translational modification

Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.

Sequence similarities

Belongs to the hantavirus envelope glycoprotein family.

Contains 1 ITAM domain.

Ontologies

Keywords
   Biological processFusion of virus membrane with host endosomal membrane
Fusion of virus membrane with host membrane
Host-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host RLR pathway by virus
Inhibition of host TRAFs by virus
Viral attachment to host cell
Viral immunoevasion
Viral penetration into host cytoplasm
Virus entry into host cell
   Cellular componentHost endoplasmic reticulum
Host Golgi apparatus
Host membrane
Membrane
Virion
   DomainSignal
Transmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfusion of virus membrane with host endosome membrane

Inferred from electronic annotation. Source: UniProtKB-KW

signal transduction

Inferred from electronic annotation. Source: InterPro

suppression by virus of host TRAF activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

virion attachment to host cell

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell Golgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

virion membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 11481125Envelope glycoprotein
PRO_0000036831
Chain24 – 658635Glycoprotein G1 By similarity
PRO_0000036832
Chain659 – 1148490Glycoprotein G2 By similarity
PRO_0000036833

Regions

Topological domain24 – 496473Lumenal Potential
Transmembrane497 – 51721Helical; Potential
Topological domain518 – 658141Cytoplasmic Potential
Topological domain659 – 1115457Lumenal Potential
Transmembrane1116 – 113621Helical; Potential
Topological domain1137 – 114812Cytoplasmic Potential
Domain621 – 64424ITAM

Sites

Site658 – 6592Cleavage; by host signal peptidase By similarity

Amino acid modifications

Glycosylation1421N-linked (GlcNAc...); by host Potential
Glycosylation3571N-linked (GlcNAc...); by host Potential
Glycosylation4091N-linked (GlcNAc...); by host Potential
Glycosylation8981N-linked (GlcNAc...); by host Potential
Glycosylation9371N-linked (GlcNAc...); by host Potential

Natural variations

Natural variant251K → R in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural variant4721M → L in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural variant5361R → K in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural variant6651D → A in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural variant8221G → E in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural variant9531L → P in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural variant9881T → I in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural variant10201H → D in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
Natural variant11451D → E in strain: isolate Sotkamo 2009/WHO Arbovirus collection.

Sequences

Sequence LengthMass (Da)Tools
P27312 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: BA606F74979EE73F

FASTA1,148126,294
        10         20         30         40         50         60 
MGKSSPVCLY LILQGLLLFD TVNAKNLNEL KMECPHTIGL GQGLVVGSVE LPPVPIQQIE 

        70         80         90        100        110        120 
SLKLESSCNF DLHTSTAGQQ SFTKWTWETK GDLAENTQAS STSFQTKSSE VNLRGLCLIP 

       130        140        150        160        170        180 
TLVVETAARM RKTIACYDLS CNQTVCQPTV YLMGPIQTCL TTKSCLLGLG DQRIQVNYER 

       190        200        210        220        230        240 
TYCVSGQLVE GVCFNPIHTM ALSQPSHTYD IVTIMVRCFL VIKKVTSGDS MKIEKNFETL 

       250        260        270        280        290        300 
VQKTGCTANG FQGYYICLIG SSSEPLYVPT LDDYRSAEVL SRMAFAPHGE DHDIEKNAVS 

       310        320        330        340        350        360 
ALRIAGKVTG KAPSTESSDT VQGIAFSGSP LYTSTGVLTA KDDPVYVWAP GIIMEGNHSV 

       370        380        390        400        410        420 
CEKKTLPLTW TGFIPLPGEI EKTTQCTVFC TLAGPGADCE AYSETGIFNI SSPTCLINRV 

       430        440        450        460        470        480 
QRFRGAEQQI KFVCQRVDMD ITVYCNGVKK VILTKTLVIG QCIYTFTSIF SMIPGIAHSL 

       490        500        510        520        530        540 
AVELCVPGLH GWATVLLLLT FCFGWVLIPT ITMILLKILI AFAYLCSKYN TDSKFRILVE 

       550        560        570        580        590        600 
KVKKEYQKTM GSMVCEVCQY ECETAKELES HRKSCSIGSC PYCLNPSEAT PSALQAHFKV 

       610        620        630        640        650        660 
CKLTSRFQEN LKKSLTMYEP MQGCYRTLSL FRYRSRFFVG LVWCMLLVLE LIVWAASAET 

       670        680        690        700        710        720 
QNLNDGWTDT AHGSGIIPMK ADLELDFSLP SSASYTYRRQ LQNPANEQEK IPFHLQISKQ 

       730        740        750        760        770        780 
VIHAEIQHLG HWMDATFNLK TAFHCYGSCE KYAYPWQTAG CFVEKDYEYE TGWGCNPPDC 

       790        800        810        820        830        840 
PGVGTGCTAC GVYLDKLKSV GKVFKIVSLR YTRKVCIQLG TGQTCKTVDS NDCLITTSVK 

       850        860        870        880        890        900 
VCLIGTISKF QPSDTLLFLG PLQQGGLIFK QWCTTTCQFG DPGDIMSTPT GMKCPELNGS 

       910        920        930        940        950        960 
FRKKCAFATT PVCQFDGNTI SGYKRMVATK DSFQSFNVTE PHISTSALEW IDLDSSLRDH 

       970        980        990       1000       1010       1020 
INVIVSRDLS FQDLSETPCQ VDLTTSATDG AWGSGVGFNL VCTVSLTECS AFLTSIKACH 

      1030       1040       1050       1060       1070       1080 
AAMCYGSTTT NLVRGQNTIH VVGKGGHSGS KFMCCHDTKC SSTGLVAAAP HLDRVTGFNQ 

      1090       1100       1110       1120       1130       1140 
ADSDKIFDDG APECGMSCWF KKLGEWVLGV LNGNWMVVAV LIALLILSIF LFALCCPRRP 


SYKKDHKP 

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References

[1]"Cloning and sequencing of Puumala virus Sotkamo strain S and M RNA segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein."
Vapalahti O.P., Kallio-Kokko H., Salonen E.M., Brummer-Korvenkontio M., Vaheri A.
J. Gen. Virol. 73:829-838(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Resequencing of the Puumala virus strain Sotkamo from the WHO Arbovirus collection."
Kurolt I.C., Paessler S., Markotic A.
Virus Genes 45:389-392(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Sotkamo 2009/WHO Arbovirus collection.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61034 Genomic RNA. Translation: CAA43369.1.
HE801634 Genomic RNA. Translation: CCH22848.1.
PIRJQ1604.
RefSeqNP_941983.1. NC_005223.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2943082.

Family and domain databases

InterProIPR016402. Envelope_gylcoprot_Hantavirus.
IPR002534. Hanta_G1.
IPR002532. Hanta_G2.
IPR012316. ITAM_motif_hantavir-typ.
[Graphical view]
PfamPF01567. Hanta_G1. 1 hit.
PF01561. Hanta_G2. 1 hit.
PF10538. ITAM_Cys-rich. 1 hit.
[Graphical view]
PIRSFPIRSF003945. M_poly_HantaV. 1 hit.
ProDomPD001813. Hanta_G2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS51056. ITAM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGP_PUUMS
AccessionPrimary (citable) accession number: P27312
Secondary accession number(s): I4EPA4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families