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Protein

Soluble pyridine nucleotide transhydrogenase

Gene

sthA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation.

Catalytic activityi

NADPH + NAD+ = NADP+ + NADH.

Cofactori

FADNote: Binds 1 FAD per subunit.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 4510FADBy similarity

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • NAD(P)+ transhydrogenase (B-specific) activity Source: UniProtKB-HAMAP
  • NAD(P)+ transhydrogenase activity Source: EcoCyc

GO - Biological processi

  • cell redox homeostasis Source: EcoCyc
  • NADP metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciEcoCyc:UDHA-MONOMER.
ECOL316407:JW5551-MONOMER.
MetaCyc:UDHA-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Soluble pyridine nucleotide transhydrogenase (EC:1.6.1.1)
Short name:
STH
Alternative name(s):
NAD(P)(+) transhydrogenase [B-specific]
Gene namesi
Name:sthA
Synonyms:sth, udhA
Ordered Locus Names:b3962, JW5551
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11428. sthA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 466465Soluble pyridine nucleotide transhydrogenasePRO_0000068061Add
BLAST

Proteomic databases

EPDiP27306.
PaxDbiP27306.
PRIDEiP27306.

Interactioni

Subunit structurei

Homooligomer; probable homooctamer.

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4263449. 8 interactions.
852757. 1 interaction.
IntActiP27306. 1 interaction.
STRINGi511145.b3962.

Structurei

3D structure databases

ProteinModelPortaliP27306.
SMRiP27306. Positions 7-464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DX1. Bacteria.
COG1249. LUCA.
HOGENOMiHOG000276708.
InParanoidiP27306.
KOiK00322.
OMAiENEEFFF.
PhylomeDBiP27306.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 3 hits.
HAMAPiMF_00247. SthA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR022962. STH.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27306-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHSYDYDAI VIGSGPGGEG AAMGLVKQGA RVAVIERYQN VGGGCTHWGT
60 70 80 90 100
IPSKALRHAV SRIIEFNQNP LYSDHSRLLR SSFADILNHA DNVINQQTRM
110 120 130 140 150
RQGFYERNHC EILQGNARFV DEHTLALDCP DGSVETLTAE KFVIACGSRP
160 170 180 190 200
YHPTDVDFTH PRIYDSDSIL SMHHEPRHVL IYGAGVIGCE YASIFRGMDV
210 220 230 240 250
KVDLINTRDR LLAFLDQEMS DSLSYHFWNS GVVIRHNEEY EKIEGCDDGV
260 270 280 290 300
IMHLKSGKKL KADCLLYANG RTGNTDSLAL QNIGLETDSR GQLKVNSMYQ
310 320 330 340 350
TAQPHVYAVG DVIGYPSLAS AAYDQGRIAA QALVKGEATA HLIEDIPTGI
360 370 380 390 400
YTIPEISSVG KTEQQLTAMK VPYEVGRAQF KHLARAQIVG MNVGTLKILF
410 420 430 440 450
HRETKEILGI HCFGERAAEI IHIGQAIMEQ KGGGNTIEYF VNTTFNYPTM
460
AEAYRVAALN GLNRLF
Length:466
Mass (Da):51,560
Last modified:January 23, 2007 - v5
Checksum:i2D7405D1E2D062FD
GO

Sequence cautioni

The sequence AAC43068 differs from that shown. Reason: Frameshift at position 15. Curated
The sequence CAA46822 differs from that shown. Reason: Frameshift at position 15. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641I → L in CAA46822 (PubMed:1447162).Curated
Sequence conflicti118 – 1181R → L in CAA46822 (PubMed:1447162).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43068.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76944.2.
AP009048 Genomic DNA. Translation: BAE77349.1.
X66026 Genomic DNA. Translation: CAA46822.1. Frameshift.
X16531 Genomic DNA. No translation available.
PIRiE65203.
RefSeqiNP_418397.2. NC_000913.3.
WP_001120810.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76944; AAC76944; b3962.
BAE77349; BAE77349; BAE77349.
GeneIDi948461.
KEGGiecj:JW5551.
eco:b3962.
PATRICi32123445. VBIEscCol129921_4083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43068.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76944.2.
AP009048 Genomic DNA. Translation: BAE77349.1.
X66026 Genomic DNA. Translation: CAA46822.1. Frameshift.
X16531 Genomic DNA. No translation available.
PIRiE65203.
RefSeqiNP_418397.2. NC_000913.3.
WP_001120810.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP27306.
SMRiP27306. Positions 7-464.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263449. 8 interactions.
852757. 1 interaction.
IntActiP27306. 1 interaction.
STRINGi511145.b3962.

Proteomic databases

EPDiP27306.
PaxDbiP27306.
PRIDEiP27306.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76944; AAC76944; b3962.
BAE77349; BAE77349; BAE77349.
GeneIDi948461.
KEGGiecj:JW5551.
eco:b3962.
PATRICi32123445. VBIEscCol129921_4083.

Organism-specific databases

EchoBASEiEB1398.
EcoGeneiEG11428. sthA.

Phylogenomic databases

eggNOGiENOG4105DX1. Bacteria.
COG1249. LUCA.
HOGENOMiHOG000276708.
InParanoidiP27306.
KOiK00322.
OMAiENEEFFF.
PhylomeDBiP27306.

Enzyme and pathway databases

BioCyciEcoCyc:UDHA-MONOMER.
ECOL316407:JW5551-MONOMER.
MetaCyc:UDHA-MONOMER.

Miscellaneous databases

PROiP27306.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 3 hits.
HAMAPiMF_00247. SthA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR022962. STH.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSTHA_ECOLI
AccessioniPrimary (citable) accession number: P27306
Secondary accession number(s): Q2M8Q7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 152 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.