Reviewed, UniProtKB/Swiss-Prot P27306 (STHA_ECOLI)

Names and origin

Protein names

Recommended name:
    Soluble pyridine nucleotide transhydrogenase
      Short name=STH
    EC=1.6.1.1
Alternative name(s):
    NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Synonyms:	sth, udhA
Ordered Locus Names:	b3962, JW5551

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation. HAMAP MF_00247
Catalytic activity	NADPH + NAD+ = NADP+ + NADH. HAMAP MF_00247
Cofactor	Binds 1 FAD per subunit. HAMAP MF_00247
Subunit structure	Homooligomer; probable homooctamer. HAMAP MF_00247
Subcellular location	Cytoplasm. HAMAP MF_00247
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Sequence caution	The sequence AAC43068.1 differs from that shown. Reason: Frameshift at position 15. The sequence CAA46822.1 differs from that shown. Reason: Frameshift at position 15.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	NADP metabolic process Inferred from electronic annotation. Source: HAMAP cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 466	465	Soluble pyridine nucleotide transhydrogenase HAMAP MF_00247		PRO_0000068061
Regions
	Nucleotide binding	36 – 45	10	FAD By similarity
Experimental info
	Sequence conflict	64	1	I → L in CAA46822. Ref.5
	Sequence conflict	118	1	R → L in CAA46822. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P27306-1 [UniParc]. Last modified January 23, 2007. Version 5. Checksum: 2D7405D1E2D062FD	FASTA	466	51,560
	10 20 30 40 50 60 MPHSYDYDAI VIGSGPGGEG AAMGLVKQGA RVAVIERYQN VGGGCTHWGT IPSKALRHAV 70 80 90 100 110 120 SRIIEFNQNP LYSDHSRLLR SSFADILNHA DNVINQQTRM RQGFYERNHC EILQGNARFV 130 140 150 160 170 180 DEHTLALDCP DGSVETLTAE KFVIACGSRP YHPTDVDFTH PRIYDSDSIL SMHHEPRHVL 190 200 210 220 230 240 IYGAGVIGCE YASIFRGMDV KVDLINTRDR LLAFLDQEMS DSLSYHFWNS GVVIRHNEEY 250 260 270 280 290 300 EKIEGCDDGV IMHLKSGKKL KADCLLYANG RTGNTDSLAL QNIGLETDSR GQLKVNSMYQ 310 320 330 340 350 360 TAQPHVYAVG DVIGYPSLAS AAYDQGRIAA QALVKGEATA HLIEDIPTGI YTIPEISSVG 370 380 390 400 410 420 KTEQQLTAMK VPYEVGRAQF KHLARAQIVG MNVGTLKILF HRETKEILGI HCFGERAAEI 430 440 450 460 IHIGQAIMEQ KGGGNTIEYF VNTTFNYPTM AEAYRVAALN GLNRLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The udhA gene of Escherichia coli encodes a soluble pyridine nucleotide transhydrogenase." Boonstra B., French C.E., Wainwright I., Bruce N.C. J. Bacteriol. 181:1030-1034(1999) [PubMed: 9922271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-27, CHARACTERIZATION.
[2]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Physical map of the oxyR-trmA region (minute 89.3) of the Escherichia coli chromosome." Gustafsson C., Warne S.R. J. Bacteriol. 174:7878-7879(1992) [PubMed: 1447162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-346. Strain: K12.
[6]	"Molecular cloning and nucleotide sequencing of oxyR, the positive regulatory gene of a regulon for an adaptive response to oxidative stress in Escherichia coli: homologies between OxyR protein and a family of bacterial activator proteins." Tao K., Makino K., Yonei S., Nakata A., Shinagawa H. Mol. Gen. Genet. 218:371-376(1989) [PubMed: 2511419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 347-466.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00006 Genomic DNA. Translation: AAC43068.1. Frameshift. U00096 Genomic DNA. Translation: AAC76944.1. Different initiation. AP009048 Genomic DNA. Translation: BAE77349.1. X66026 Genomic DNA. Translation: CAA46822.1. Frameshift. X16531 Genomic DNA. No translation available.
PIR	E65203.
RefSeq	AP_003848.1. NP_418397.2.
3D structure databases
HSSP	HSSP built from PDB template 1LPF based on UniProtKB P14218.
ModBase	Search...
Genome annotation databases
GeneID	948461.
GenomeReviews	Gene locus JW5551 in contig AP009048_GR. Gene locus b3962 in contig U00096_GR.
KEGG	ecj:JW5551. eco:b3962.
Organism-specific databases
EchoBASE	EB1398.
EcoGene	EG11428. sthA.
CMR	Search...
Phylogenomic databases
HOGENOM	P27306.
OMA	P27306. GEGNTIE.
Enzyme and pathway databases
BioCyc	EcoCyc:UDHA-MON. MetaCyc:UDHA-MON.
Family and domain databases
HAMAP	MF_00247. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

STHA_ECOLI

Accession

Primary (citable) accession number: P27306
Secondary accession number(s): Q2M8Q7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 90 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)