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Protein

Multidrug export protein EmrA

Gene

emrA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the tripartite efflux system EmrAB-TolC, which confers resistance to antibiotics such as CCCP, FCCP, 2,4-dinitrophenol and nalidixic acid. EmrA is a drug-binding protein that provides a physical link between EmrB and TolC.2 Publications

GO - Molecular functioni

  • drug transmembrane transporter activity Source: InterPro
  • identical protein binding Source: IntAct

GO - Biological processi

Keywordsi

Biological processAntibiotic resistance, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11354-MONOMER

Protein family/group databases

TCDBi8.A.1.1.1 the membrane fusion protein (mfp) family

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug export protein EmrA
Gene namesi
Name:emrA
Ordered Locus Names:b2685, JW2660
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG11354 emrA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24CytoplasmicSequence analysisAdd BLAST24
Transmembranei25 – 45HelicalSequence analysisAdd BLAST21
Topological domaini46 – 390PeriplasmicSequence analysisAdd BLAST345

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intrinsic component of periplasmic side of plasma membrane Source: EcoCyc
  • intrinsic component of plasma membrane Source: EcoliWiki

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002018691 – 390Multidrug export protein EmrAAdd BLAST390

Proteomic databases

PaxDbiP27303
PRIDEiP27303

Interactioni

Subunit structurei

Homodimer and homotrimer. Part of the tripartite efflux system EmrAB-TolC, which is composed of an inner membrane transporter, EmrB, a periplasmic membrane fusion protein, EmrA, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes. Interacts with EmrB. EmrAB complex forms a dimer in vitro.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-1119547,EBI-1119547

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4262946, 219 interactors
DIPiDIP-9502N
IntActiP27303, 3 interactors
STRINGi316385.ECDH10B_2852

Structurei

3D structure databases

ProteinModelPortaliP27303
SMRiP27303
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili120 – 180Sequence analysisAdd BLAST61

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CW7 Bacteria
COG1566 LUCA
HOGENOMiHOG000112072
InParanoidiP27303
KOiK03543
OMAiMMVETEA
PhylomeDBiP27303

Family and domain databases

InterProiView protein in InterPro
IPR005694 Emr
IPR032317 HlyD_D23
IPR006143 RND_pump_MFP
PfamiView protein in Pfam
PF00529 HlyD, 1 hit
PF16576 HlyD_D23, 1 hit
TIGRFAMsiTIGR00998 8a0101, 1 hit

Sequencei

Sequence statusi: Complete.

P27303-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSANAETQTP QQPVKKSGKR KRLLLLLTLL FIIIAVAIGI YWFLVLRHFE
60 70 80 90 100
ETDDAYVAGN QIQIMSQVSG SVTKVWADNT DFVKEGDVLV TLDPTDARQA
110 120 130 140 150
FEKAKTALAS SVRQTHQLMI NSKQLQANIE VQKIALAKAQ SDYNRRVPLG
160 170 180 190 200
NANLIGREEL QHARDAVTSA QAQLDVAIQQ YNANQAMILG TKLEDQPAVQ
210 220 230 240 250
QAATEVRNAW LALERTRIIS PMTGYVSRRA VQPGAQISPT TPLMAVVPAT
260 270 280 290 300
NMWVDANFKE TQIANMRIGQ PVTITTDIYG DDVKYTGKVV GLDMGTGSAF
310 320 330 340 350
SLLPAQNATG NWIKVVQRLP VRIELDQKQL EQYPLRIGLS TLVSVNTTNR
360 370 380 390
DGQVLANKVR STPVAVSTAR EISLAPVNKL IDDIVKANAG
Length:390
Mass (Da):42,736
Last modified:November 1, 1997 - v2
Checksum:i0FB9AE8C20A270F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62I → M in AAA23724 (PubMed:1409590).Curated1
Sequence conflicti138K → Q in AAA23724 (PubMed:1409590).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86657 Genomic DNA Translation: AAA23724.1
U00096 Genomic DNA Translation: AAC75732.1
AP009048 Genomic DNA Translation: BAA16547.1
PIRiF65048
RefSeqiNP_417170.1, NC_000913.3
WP_001326681.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75732; AAC75732; b2685
BAA16547; BAA16547; BAA16547
GeneIDi947166
KEGGiecj:JW2660
eco:b2685
PATRICifig|1411691.4.peg.4054

Similar proteinsi

Entry informationi

Entry nameiEMRA_ECOLI
AccessioniPrimary (citable) accession number: P27303
Secondary accession number(s): P77356
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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