P27300 (LPXK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Synonyms:	ycaH
Ordered Locus Names:	b0915, JW0898

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA). HAMAP-Rule MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP-Rule MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP-Rule MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from direct assay Ref.5. Source: EcoliWiki lipopolysaccharide core region biosynthetic process Inferred from mutant phenotype Ref.6. Source: EcoCyc
Cellular_component	membrane Inferred from direct assay Ref.5. Source: EcoliWiki
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tetraacyldisaccharide 4'-kinase activity Inferred from direct assay Ref.5. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Tetraacyldisaccharide 4'-kinase HAMAP-Rule MF_00409		PRO_0000190924
Regions
	Nucleotide binding	55 – 62	8	ATP Potential
Experimental info
	Sequence conflict	158	1	D → N in CAA77840. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P27300 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: B9692F479F22305D	FASTA	328	35,589
	10 20 30 40 50 60 MIEKIWSGES PLWRLLLPLS WLYGLVSGAI RLCYKLKLKR AWRAPVPVVV VGNLTAGGNG 70 80 90 100 110 120 KTPVVVWLVE QLQQRGIRVG VVSRGYGGKA ESYPLLLSAD TTTAQAGDEP VLIYQRTDAP 130 140 150 160 170 180 VAVSPVRSDA VKAILAQHPD VQIIVTDDGL QHYRLARDVE IVVIDGVRRF GNGWWLPAGP 190 200 210 220 230 240 MRERAGRLKS VDAVIVNGGV PRSGEIPMHL LPGQAVNLRT GTRCDVAQLE HVVAMAGIGH 250 260 270 280 290 300 PPRFFATLKM CGVQPEKCVP LADHQSLNHA DVSALVSAGQ TLVMTEKDAV KCRAFAEENW 310 320 WYLPVDAQLS GDEPAKLLTQ LTLLASGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The essential Escherichia coli msbA gene, a multicopy suppressor of null mutations in the htrB gene, is related to the universally conserved family of ATP-dependent translocators." Karow M.L., Georgopoulos C. Mol. Microbiol. 7:69-79(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Identification of the gene encoding the Escherichia coli lipid A 4'-kinase. Facile phosphorylation of endotoxin analogs with recombinant LpxK." Garrett T.A., Kadrmas J.L., Raetz C.R.H. J. Biol. Chem. 272:21855-21864(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Accumulation of a lipid A precursor lacking the 4'-phosphate following inactivation of the Escherichia coli lpxK gene." Garrett T.A., Que N.L., Raetz C.R.H. J. Biol. Chem. 273:12457-12465(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z11796 Genomic DNA. Translation: CAA77840.1. U00096 Genomic DNA. Translation: AAC74001.1. AP009048 Genomic DNA. Translation: BAA35661.1.
PIR	B64831.
RefSeq	NP_415435.1. NC_000913.2. YP_489187.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P27300.
SMR	P27300. Positions 16-318.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10126N.
IntAct	P27300. 1 interaction.
STRING	511145.b0915.
Proteomic databases
PaxDb	P27300.
PRIDE	P27300.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74001; AAC74001; b0915. BAA35661; BAA35661; BAA35661.
GeneID	12931020. 945526.
KEGG	ecj:Y75_p0887. eco:b0915.
PATRIC	32117043. VBIEscCol129921_0946.
Organism-specific databases
EchoBASE	EB1381.
EcoGene	EG11409. lpxK.
Phylogenomic databases
eggNOG	COG1663.
HOGENOM	HOG000004953.
KO	K00912.
OMA	DEPVLIH.
ProtClustDB	PRK00652.
Enzyme and pathway databases
BioCyc	EcoCyc:TETRAACYLDISACC4KIN-MONOMER. ECOL316407:JW0898-MONOMER. MetaCyc:TETRAACYLDISACC4KIN-MONOMER.
UniPathway	UPA00359; UER00482.
Gene expression databases
Genevestigator	P27300.
Family and domain databases
HAMAP	MF_00409. LpxK.
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. lpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_ECOLI

Accession

Primary (citable) accession number: P27300
Secondary accession number(s): P75842, Q9R7Q6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program