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Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).2 Publications

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).1 Publication

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD)
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 62ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: EcoCyc
  • tetraacyldisaccharide 4'-kinase activity Source: EcoliWiki

GO - Biological processi

  • lipid A biosynthetic process Source: EcoliWiki
  • lipopolysaccharide core region biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:TETRAACYLDISACC4KIN-MONOMER
MetaCyc:TETRAACYLDISACC4KIN-MONOMER
UniPathwayiUPA00359; UER00482

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinase (EC:2.7.1.1301 Publication)
Alternative name(s):
Lipid A 4'-kinase
Gene namesi
Name:lpxK
Synonyms:ycaH
Ordered Locus Names:b0915, JW0898
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11409 lpxK

Subcellular locationi

GO - Cellular componenti

  • membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001909241 – 328Tetraacyldisaccharide 4'-kinaseAdd BLAST328

Proteomic databases

PaxDbiP27300
PRIDEiP27300

Interactioni

Protein-protein interaction databases

BioGridi4260014, 329 interactors
DIPiDIP-10126N
IntActiP27300, 1 interactor
STRINGi316385.ECDH10B_0985

Structurei

3D structure databases

ProteinModelPortaliP27300
SMRiP27300
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.Curated

Phylogenomic databases

eggNOGiENOG4105CHD Bacteria
COG1663 LUCA
HOGENOMiHOG000004953
InParanoidiP27300
KOiK00912
OMAiMDDGFQN
PhylomeDBiP27300

Family and domain databases

HAMAPiMF_00409 LpxK, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR003758 Tetraacyldisaccharide_4-kinase
PANTHERiPTHR42724 PTHR42724, 1 hit
PfamiView protein in Pfam
PF02606 LpxK, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00682 lpxK, 1 hit

Sequencei

Sequence statusi: Complete.

P27300-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEKIWSGES PLWRLLLPLS WLYGLVSGAI RLCYKLKLKR AWRAPVPVVV
60 70 80 90 100
VGNLTAGGNG KTPVVVWLVE QLQQRGIRVG VVSRGYGGKA ESYPLLLSAD
110 120 130 140 150
TTTAQAGDEP VLIYQRTDAP VAVSPVRSDA VKAILAQHPD VQIIVTDDGL
160 170 180 190 200
QHYRLARDVE IVVIDGVRRF GNGWWLPAGP MRERAGRLKS VDAVIVNGGV
210 220 230 240 250
PRSGEIPMHL LPGQAVNLRT GTRCDVAQLE HVVAMAGIGH PPRFFATLKM
260 270 280 290 300
CGVQPEKCVP LADHQSLNHA DVSALVSAGQ TLVMTEKDAV KCRAFAEENW
310 320
WYLPVDAQLS GDEPAKLLTQ LTLLASGN
Length:328
Mass (Da):35,589
Last modified:July 15, 1998 - v2
Checksum:iB9692F479F22305D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti158D → N in CAA77840 (PubMed:8094880).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11796 Genomic DNA Translation: CAA77840.1
U00096 Genomic DNA Translation: AAC74001.1
AP009048 Genomic DNA Translation: BAA35661.1
PIRiB64831
RefSeqiNP_415435.1, NC_000913.3
WP_000570539.1, NZ_CP010440.1
WP_000570544.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74001; AAC74001; b0915
BAA35661; BAA35661; BAA35661
GeneIDi945526
KEGGiecj:JW0898
eco:b0915
PATRICifig|511145.12.peg.946

Similar proteinsi

Entry informationi

Entry nameiLPXK_ECOLI
AccessioniPrimary (citable) accession number: P27300
Secondary accession number(s): P75842, Q9R7Q6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 15, 1998
Last modified: March 28, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health