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Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).2 Publications

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).1 Publication

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD)
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 62ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • kinase activity Source: EcoCyc
  • tetraacyldisaccharide 4'-kinase activity Source: EcoliWiki

GO - Biological processi

  • lipid A biosynthetic process Source: EcoliWiki
  • lipopolysaccharide core region biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:TETRAACYLDISACC4KIN-MONOMER.
ECOL316407:JW0898-MONOMER.
MetaCyc:TETRAACYLDISACC4KIN-MONOMER.
UniPathwayiUPA00359; UER00482.

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinase (EC:2.7.1.1301 Publication)
Alternative name(s):
Lipid A 4'-kinase
Gene namesi
Name:lpxK
Synonyms:ycaH
Ordered Locus Names:b0915, JW0898
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11409. lpxK.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001909241 – 328Tetraacyldisaccharide 4'-kinaseAdd BLAST328

Proteomic databases

PaxDbiP27300.
PRIDEiP27300.

Interactioni

Protein-protein interaction databases

BioGridi4260014. 271 interactors.
DIPiDIP-10126N.
IntActiP27300. 1 interactor.
STRINGi511145.b0915.

Structurei

3D structure databases

ProteinModelPortaliP27300.
SMRiP27300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.Curated

Phylogenomic databases

eggNOGiENOG4105CHD. Bacteria.
COG1663. LUCA.
HOGENOMiHOG000004953.
InParanoidiP27300.
KOiK00912.
OMAiPTVIYLI.
PhylomeDBiP27300.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
3.40.50.300. 2 hits.
HAMAPiMF_00409. LpxK. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
[Graphical view]
PfamiPF02606. LpxK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00682. lpxK. 1 hit.

Sequencei

Sequence statusi: Complete.

P27300-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEKIWSGES PLWRLLLPLS WLYGLVSGAI RLCYKLKLKR AWRAPVPVVV
60 70 80 90 100
VGNLTAGGNG KTPVVVWLVE QLQQRGIRVG VVSRGYGGKA ESYPLLLSAD
110 120 130 140 150
TTTAQAGDEP VLIYQRTDAP VAVSPVRSDA VKAILAQHPD VQIIVTDDGL
160 170 180 190 200
QHYRLARDVE IVVIDGVRRF GNGWWLPAGP MRERAGRLKS VDAVIVNGGV
210 220 230 240 250
PRSGEIPMHL LPGQAVNLRT GTRCDVAQLE HVVAMAGIGH PPRFFATLKM
260 270 280 290 300
CGVQPEKCVP LADHQSLNHA DVSALVSAGQ TLVMTEKDAV KCRAFAEENW
310 320
WYLPVDAQLS GDEPAKLLTQ LTLLASGN
Length:328
Mass (Da):35,589
Last modified:July 15, 1998 - v2
Checksum:iB9692F479F22305D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti158D → N in CAA77840 (PubMed:8094880).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11796 Genomic DNA. Translation: CAA77840.1.
U00096 Genomic DNA. Translation: AAC74001.1.
AP009048 Genomic DNA. Translation: BAA35661.1.
PIRiB64831.
RefSeqiNP_415435.1. NC_000913.3.
WP_000570539.1. NZ_CP010440.1.
WP_000570544.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74001; AAC74001; b0915.
BAA35661; BAA35661; BAA35661.
GeneIDi945526.
KEGGiecj:JW0898.
eco:b0915.
PATRICi32117043. VBIEscCol129921_0946.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11796 Genomic DNA. Translation: CAA77840.1.
U00096 Genomic DNA. Translation: AAC74001.1.
AP009048 Genomic DNA. Translation: BAA35661.1.
PIRiB64831.
RefSeqiNP_415435.1. NC_000913.3.
WP_000570539.1. NZ_CP010440.1.
WP_000570544.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP27300.
SMRiP27300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260014. 271 interactors.
DIPiDIP-10126N.
IntActiP27300. 1 interactor.
STRINGi511145.b0915.

Proteomic databases

PaxDbiP27300.
PRIDEiP27300.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74001; AAC74001; b0915.
BAA35661; BAA35661; BAA35661.
GeneIDi945526.
KEGGiecj:JW0898.
eco:b0915.
PATRICi32117043. VBIEscCol129921_0946.

Organism-specific databases

EchoBASEiEB1381.
EcoGeneiEG11409. lpxK.

Phylogenomic databases

eggNOGiENOG4105CHD. Bacteria.
COG1663. LUCA.
HOGENOMiHOG000004953.
InParanoidiP27300.
KOiK00912.
OMAiPTVIYLI.
PhylomeDBiP27300.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00482.
BioCyciEcoCyc:TETRAACYLDISACC4KIN-MONOMER.
ECOL316407:JW0898-MONOMER.
MetaCyc:TETRAACYLDISACC4KIN-MONOMER.

Miscellaneous databases

PROiP27300.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
3.40.50.300. 2 hits.
HAMAPiMF_00409. LpxK. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
[Graphical view]
PfamiPF02606. LpxK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00682. lpxK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPXK_ECOLI
AccessioniPrimary (citable) accession number: P27300
Secondary accession number(s): P75842, Q9R7Q6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 15, 1998
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.