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Protein

Oligopeptidase A

Gene

prlC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

May play a specific role in the degradation of signal peptides after they are released from precursor forms of secreted proteins. Can cleave N-acetyl-L-Ala4.

Catalytic activityi

Hydrolysis of oligopeptides, with broad specificity. Gly or Ala commonly occur as P1 or P1' residues, but more distant residues are also important, as is shown by the fact that Z-Gly-Pro-Gly-|-Gly-Pro-Ala is cleaved, but not Z-(Gly)5.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi469Zinc; catalyticPROSITE-ProRule annotation1
Active sitei470PROSITE-ProRule annotation1
Metal bindingi473Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi476Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: GO_Central
  • peptidase activity Source: EcoCyc

GO - Biological processi

  • DNA replication Source: EcoCyc
  • signal peptide processing Source: EcoliWiki

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG11441-MONOMER
MetaCyc:EG11441-MONOMER

Protein family/group databases

MEROPSiM03.004

Names & Taxonomyi

Protein namesi
Recommended name:
Oligopeptidase A (EC:3.4.24.70)
Gene namesi
Name:prlC
Synonyms:opdA
Ordered Locus Names:b3498, JW3465
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11441 prlC

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000781591 – 680Oligopeptidase AAdd BLAST680

Proteomic databases

EPDiP27298
PaxDbiP27298
PRIDEiP27298

Interactioni

Protein-protein interaction databases

BioGridi4262506, 55 interactors
DIPiDIP-10566N
IntActiP27298, 4 interactors
STRINGi316385.ECDH10B_3674

Structurei

3D structure databases

ProteinModelPortaliP27298
SMRiP27298
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Phylogenomic databases

eggNOGiENOG4105DGW Bacteria
COG0339 LUCA
HOGENOMiHOG000245986
InParanoidiP27298
KOiK01414
OMAiINGVAWD
PhylomeDBiP27298

Family and domain databases

CDDicd06456 M3A_DCP, 1 hit
Gene3Di1.10.1370.10, 2 hits
InterProiView protein in InterPro
IPR034005 M3A_DCP
IPR024077 Neurolysin/TOP_dom2
IPR001567 Pept_M3A_M3B
PfamiView protein in Pfam
PF01432 Peptidase_M3, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

P27298-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNPLLTPFE LPPFSKILPE HVVPAVTKAL NDCRENVERV VAQGAPYTWE
60 70 80 90 100
NLCQPLAEVD DVLGRIFSPV SHLNSVKNSP ELREAYEQTL PLLSEYSTWV
110 120 130 140 150
GQHEGLYKAY RDLRDGDHYA TLNTAQKKAV DNALRDFELS GIGLPKEKQQ
160 170 180 190 200
RYGEIATRLS ELGNQYSNNV LDATMGWTKL VTDEAELAGM PESALAAAKA
210 220 230 240 250
QAEAKELEGY LLTLDIPSYL PVMTYCDNQA LREEMYRAYS TRASDQGPNA
260 270 280 290 300
GKWDNSKVME EILALRHELA QLLGFENYAF KSLATKMAEN PQQVLDFLTD
310 320 330 340 350
LAKRARPQGE KELAQLRAFA KAEFGVDELQ PWDIAYYSEK QKQHLYSISD
360 370 380 390 400
EQLRPYFPEN KAVNGLFEVV KRIYGITAKE RKDVDVWHPD VRFFELYDEN
410 420 430 440 450
NELRGSFYLD LYARENKRGG AWMDDCVGQM RKADGSLQKP VAYLTCNFNR
460 470 480 490 500
PVNGKPALFT HDEVITLFHE FGHGLHHMLT RIETAGVSGI SGVPWDAVEL
510 520 530 540 550
PSQFMENWCW EPEALAFISG HYETGEPLPK ELLDKMLAAK NYQAALFILR
560 570 580 590 600
QLEFGLFDFR LHAEFRPDQG AKILETLAEI KKLVAVVPSP SWGRFPHAFS
610 620 630 640 650
HIFAGGYAAG YYSYLWADVL AADAFSRFEE EGIFNRETGQ SFLDNILSRG
660 670 680
GSEEPMDLFK RFRGREPQLD AMLEHYGIKG
Length:680
Mass (Da):77,167
Last modified:October 1, 1994 - v3
Checksum:i41E338F1BFFBF906
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211 – 212LL → FV in AAA16155 (PubMed:1325967).Curated2
Sequence conflicti265 – 266LR → AA in AAA16155 (PubMed:1325967).Curated2
Sequence conflicti390D → H in AAA16155 (PubMed:1325967).Curated1
Sequence conflicti406S → T in AAA16155 (PubMed:1325967).Curated1
Sequence conflicti516A → G in AAA16155 (PubMed:1325967).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93984 Unassigned DNA Translation: AAA16155.1
U00039 Genomic DNA Translation: AAB18474.1
U00096 Genomic DNA Translation: AAC76523.1
AP009048 Genomic DNA Translation: BAE77796.1
PIRiS47718
RefSeqiNP_417955.1, NC_000913.3
WP_001298719.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76523; AAC76523; b3498
BAE77796; BAE77796; BAE77796
GeneIDi948016
KEGGiecj:JW3465
eco:b3498
PATRICifig|511145.12.peg.3600

Similar proteinsi

Entry informationi

Entry nameiOPDA_ECOLI
AccessioniPrimary (citable) accession number: P27298
Secondary accession number(s): Q2M7G0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1994
Last modified: March 28, 2018
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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