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Protein

Oligopeptidase A

Gene

prlC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

May play a specific role in the degradation of signal peptides after they are released from precursor forms of secreted proteins. Can cleave N-acetyl-L-Ala4.

Catalytic activityi

Hydrolysis of oligopeptides, with broad specificity. Gly or Ala commonly occur as P1 or P1' residues, but more distant residues are also important, as is shown by the fact that Z-Gly-Pro-Gly-|-Gly-Pro-Ala is cleaved, but not Z-(Gly)5.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi469Zinc; catalyticPROSITE-ProRule annotation1
Active sitei470PROSITE-ProRule annotation1
Metal bindingi473Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi476Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: GO_Central
  • peptidase activity Source: EcoCyc

GO - Biological processi

  • DNA replication Source: EcoCyc
  • signal peptide processing Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG11441-MONOMER.
ECOL316407:JW3465-MONOMER.
MetaCyc:EG11441-MONOMER.

Protein family/group databases

MEROPSiM03.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligopeptidase A (EC:3.4.24.70)
Gene namesi
Name:prlC
Synonyms:opdA
Ordered Locus Names:b3498, JW3465
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11441. prlC.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000781591 – 680Oligopeptidase AAdd BLAST680

Proteomic databases

EPDiP27298.
PaxDbiP27298.
PRIDEiP27298.

Interactioni

Protein-protein interaction databases

BioGridi4262506. 8 interactors.
DIPiDIP-10566N.
IntActiP27298. 4 interactors.
MINTiMINT-1234260.
STRINGi511145.b3498.

Structurei

3D structure databases

ProteinModelPortaliP27298.
SMRiP27298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Phylogenomic databases

eggNOGiENOG4105DGW. Bacteria.
COG0339. LUCA.
HOGENOMiHOG000245986.
InParanoidiP27298.
KOiK01414.
OMAiVMRPPAY.
PhylomeDBiP27298.

Family and domain databases

Gene3Di1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27298-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNPLLTPFE LPPFSKILPE HVVPAVTKAL NDCRENVERV VAQGAPYTWE
60 70 80 90 100
NLCQPLAEVD DVLGRIFSPV SHLNSVKNSP ELREAYEQTL PLLSEYSTWV
110 120 130 140 150
GQHEGLYKAY RDLRDGDHYA TLNTAQKKAV DNALRDFELS GIGLPKEKQQ
160 170 180 190 200
RYGEIATRLS ELGNQYSNNV LDATMGWTKL VTDEAELAGM PESALAAAKA
210 220 230 240 250
QAEAKELEGY LLTLDIPSYL PVMTYCDNQA LREEMYRAYS TRASDQGPNA
260 270 280 290 300
GKWDNSKVME EILALRHELA QLLGFENYAF KSLATKMAEN PQQVLDFLTD
310 320 330 340 350
LAKRARPQGE KELAQLRAFA KAEFGVDELQ PWDIAYYSEK QKQHLYSISD
360 370 380 390 400
EQLRPYFPEN KAVNGLFEVV KRIYGITAKE RKDVDVWHPD VRFFELYDEN
410 420 430 440 450
NELRGSFYLD LYARENKRGG AWMDDCVGQM RKADGSLQKP VAYLTCNFNR
460 470 480 490 500
PVNGKPALFT HDEVITLFHE FGHGLHHMLT RIETAGVSGI SGVPWDAVEL
510 520 530 540 550
PSQFMENWCW EPEALAFISG HYETGEPLPK ELLDKMLAAK NYQAALFILR
560 570 580 590 600
QLEFGLFDFR LHAEFRPDQG AKILETLAEI KKLVAVVPSP SWGRFPHAFS
610 620 630 640 650
HIFAGGYAAG YYSYLWADVL AADAFSRFEE EGIFNRETGQ SFLDNILSRG
660 670 680
GSEEPMDLFK RFRGREPQLD AMLEHYGIKG
Length:680
Mass (Da):77,167
Last modified:October 1, 1994 - v3
Checksum:i41E338F1BFFBF906
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211 – 212LL → FV in AAA16155 (PubMed:1325967).Curated2
Sequence conflicti265 – 266LR → AA in AAA16155 (PubMed:1325967).Curated2
Sequence conflicti390D → H in AAA16155 (PubMed:1325967).Curated1
Sequence conflicti406S → T in AAA16155 (PubMed:1325967).Curated1
Sequence conflicti516A → G in AAA16155 (PubMed:1325967).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93984 Unassigned DNA. Translation: AAA16155.1.
U00039 Genomic DNA. Translation: AAB18474.1.
U00096 Genomic DNA. Translation: AAC76523.1.
AP009048 Genomic DNA. Translation: BAE77796.1.
PIRiS47718.
RefSeqiNP_417955.1. NC_000913.3.
WP_001298719.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76523; AAC76523; b3498.
BAE77796; BAE77796; BAE77796.
GeneIDi948016.
KEGGiecj:JW3465.
eco:b3498.
PATRICi32122446. VBIEscCol129921_3600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93984 Unassigned DNA. Translation: AAA16155.1.
U00039 Genomic DNA. Translation: AAB18474.1.
U00096 Genomic DNA. Translation: AAC76523.1.
AP009048 Genomic DNA. Translation: BAE77796.1.
PIRiS47718.
RefSeqiNP_417955.1. NC_000913.3.
WP_001298719.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP27298.
SMRiP27298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262506. 8 interactors.
DIPiDIP-10566N.
IntActiP27298. 4 interactors.
MINTiMINT-1234260.
STRINGi511145.b3498.

Protein family/group databases

MEROPSiM03.004.

Proteomic databases

EPDiP27298.
PaxDbiP27298.
PRIDEiP27298.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76523; AAC76523; b3498.
BAE77796; BAE77796; BAE77796.
GeneIDi948016.
KEGGiecj:JW3465.
eco:b3498.
PATRICi32122446. VBIEscCol129921_3600.

Organism-specific databases

EchoBASEiEB1411.
EcoGeneiEG11441. prlC.

Phylogenomic databases

eggNOGiENOG4105DGW. Bacteria.
COG0339. LUCA.
HOGENOMiHOG000245986.
InParanoidiP27298.
KOiK01414.
OMAiVMRPPAY.
PhylomeDBiP27298.

Enzyme and pathway databases

BioCyciEcoCyc:EG11441-MONOMER.
ECOL316407:JW3465-MONOMER.
MetaCyc:EG11441-MONOMER.

Miscellaneous databases

PROiP27298.

Family and domain databases

Gene3Di1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOPDA_ECOLI
AccessioniPrimary (citable) accession number: P27298
Secondary accession number(s): Q2M7G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.