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Protein

Probable ATP-dependent helicase DinG

Gene

dinG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable helicase involved in DNA repair and perhaps also replication.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi120Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi194Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi199Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi205Iron-sulfur (4Fe-4S)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi54 – 61ATPCurated8

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • ATPase activity Source: EcoliWiki
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent DNA/RNA helicase activity Source: EcoCyc
  • ATP-dependent DNA helicase activity Source: EcoCyc
  • DNA binding Source: UniProtKB-KW
  • DNA helicase activity Source: EcoliWiki
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • DNA duplex unwinding Source: EcoCyc
  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • SOS response Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

4Fe-4S, ATP-binding, DNA-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11357-MONOMER.
ECOL316407:JW0784-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent helicase DinG (EC:3.6.4.12)
Alternative name(s):
DNA-damage-inducible protein G
Gene namesi
Name:dinG
Synonyms:rarB
Ordered Locus Names:b0799, JW0784
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11357. dinG.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi120C → S: Abolishes iron-sulfur-binding. 1 Publication1
Mutagenesisi194C → S: Abolishes iron-sulfur-binding. 1 Publication1
Mutagenesisi199C → S: Abolishes iron-sulfur-binding. 1 Publication1
Mutagenesisi205C → S: Abolishes iron-sulfur-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001019961 – 716Probable ATP-dependent helicase DinGAdd BLAST716

Proteomic databases

PaxDbiP27296.
PRIDEiP27296.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ssbP0AGE02EBI-1114590,EBI-1118620

Protein-protein interaction databases

BioGridi4259964. 118 interactors.
IntActiP27296. 22 interactors.
MINTiMINT-8393557.
STRINGi511145.b0799.

Structurei

3D structure databases

ProteinModelPortaliP27296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 294Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST278

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi248 – 251DEGH box4

Domaini

4Fe-4S iron-sulfur-binding is required for protein stability and helicase activity. The cluster is modified by nitric oxide, forming the DinG-bound dinitrosyl iron complex with the inactivation of helicase activity (PubMed:19074432).1 Publication

Sequence similaritiesi

Belongs to the helicase family. DinG subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DVT. Bacteria.
COG1199. LUCA.
HOGENOMiHOG000242573.
InParanoidiP27296.
KOiK03722.
OMAiCAGIRVC.
PhylomeDBiP27296.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR010614. DEAD_2.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTAALKAQ IAAWYKALQE QIPDFIPRAP QRQMIADVAK TLAGEEGRHL
60 70 80 90 100
AIEAPTGVGK TLSYLIPGIA IAREEQKTLV VSTANVALQD QIYSKDLPLL
110 120 130 140 150
KKIIPDLKFT AAFGRGRYVC PRNLTALAST EPTQQDLLAF LDDELTPNNQ
160 170 180 190 200
EEQKRCAKLK GDLDTYKWDG LRDHTDIAID DDLWRRLSTD KASCLNRNCY
210 220 230 240 250
YYRECPFFVA RREIQEAEVV VANHALVMAA MESEAVLPDP KNLLLVLDEG
260 270 280 290 300
HHLPDVARDA LEMSAEITAP WYRLQLDLFT KLVATCMEQF RPKTIPPLAI
310 320 330 340 350
PERLNAHCEE LYELIASLNN ILNLYMPAGQ EAEHRFAMGE LPDEVLEICQ
360 370 380 390 400
RLAKLTEMLR GLAELFLNDL SEKTGSHDIV RLHRLILQMN RALGMFEAQS
410 420 430 440 450
KLWRLASLAQ SSGAPVTKWA TREEREGQLH LWFHCVGIRV SDQLERLLWR
460 470 480 490 500
SIPHIIVTSA TLRSLNSFSR LQEMSGLKEK AGDRFVALDS PFNHCEQGKI
510 520 530 540 550
VIPRMRVEPS IDNEEQHIAE MAAFFRKQVE SKKHLGMLVL FASGRAMQRF
560 570 580 590 600
LDYVTDLRLM LLVQGDQPRY RLVELHRKRV ANGERSVLVG LQSFAEGLDL
610 620 630 640 650
KGDLLSQVHI HKIAFPPIDS PVVITEGEWL KSLNRYPFEV QSLPSASFNL
660 670 680 690 700
IQQVGRLIRS HGCWGEVVIY DKRLLTKNYG KRLLDALPVF PIEQPEVPEG
710
IVKKKEKTKS PRRRRR
Length:716
Mass (Da):81,440
Last modified:June 1, 1994 - v3
Checksum:i63E6767E8EAA67D2
GO

Sequence cautioni

The sequence AAA23685 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti513N → H in AAA23685 (PubMed:1629168).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02123 Genomic DNA. Translation: AAA53655.1.
M81935 Genomic DNA. Translation: AAA23685.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73886.1.
AP009048 Genomic DNA. Translation: BAA35465.1.
PIRiG64816.
RefSeqiNP_415320.1. NC_000913.3.
WP_001340191.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73886; AAC73886; b0799.
BAA35465; BAA35465; BAA35465.
GeneIDi945431.
KEGGiecj:JW0784.
eco:b0799.
PATRICi32116801. VBIEscCol129921_0826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02123 Genomic DNA. Translation: AAA53655.1.
M81935 Genomic DNA. Translation: AAA23685.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73886.1.
AP009048 Genomic DNA. Translation: BAA35465.1.
PIRiG64816.
RefSeqiNP_415320.1. NC_000913.3.
WP_001340191.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP27296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259964. 118 interactors.
IntActiP27296. 22 interactors.
MINTiMINT-8393557.
STRINGi511145.b0799.

Proteomic databases

PaxDbiP27296.
PRIDEiP27296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73886; AAC73886; b0799.
BAA35465; BAA35465; BAA35465.
GeneIDi945431.
KEGGiecj:JW0784.
eco:b0799.
PATRICi32116801. VBIEscCol129921_0826.

Organism-specific databases

EchoBASEiEB1332.
EcoGeneiEG11357. dinG.

Phylogenomic databases

eggNOGiENOG4105DVT. Bacteria.
COG1199. LUCA.
HOGENOMiHOG000242573.
InParanoidiP27296.
KOiK03722.
OMAiCAGIRVC.
PhylomeDBiP27296.

Enzyme and pathway databases

BioCyciEcoCyc:EG11357-MONOMER.
ECOL316407:JW0784-MONOMER.

Miscellaneous databases

PROiP27296.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR010614. DEAD_2.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDING_ECOLI
AccessioniPrimary (citable) accession number: P27296
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.