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Protein

CD59 glycoprotein

Gene

Cd59

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potent inhibitor of the complement membrane attack complex (MAC) action. Acts at or after the C5b-8 stage of MAC assembly.

GO - Molecular functioni

  • complement binding Source: RGD

GO - Biological processi

  • cell surface receptor signaling pathway Source: GO_Central
  • negative regulation of activation of membrane attack complex Source: RGD
  • negative regulation of apoptotic process Source: RGD
  • positive regulation of T cell proliferation Source: RGD
  • regulation of complement activation Source: RGD
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-RNO-5694530. Cargo concentration in the ER.
R-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-977606. Regulation of Complement cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
CD59 glycoprotein
Alternative name(s):
MAC-inhibitory protein
Short name:
MAC-IP
Membrane attack complex inhibition factor
Short name:
MACIF
Protectin
CD_antigen: CD59
Gene namesi
Name:Cd59
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi2311. Cd59.

Subcellular locationi

GO - Cellular componenti

  • anchored component of external side of plasma membrane Source: Ensembl
  • cell surface Source: GO_Central
  • compact myelin Source: RGD
  • extracellular exosome Source: Ensembl
  • extracellular space Source: RGD
  • focal adhesion Source: Ensembl
  • plasma membrane Source: RGD
  • sarcolemma Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22222 PublicationsAdd
BLAST
Chaini23 – 10179CD59 glycoproteinPRO_0000036124Add
BLAST
Propeptidei102 – 12625Removed in mature formBy similarityPRO_0000036125Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi25 ↔ 48By similarity
Disulfide bondi28 ↔ 35By similarity
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence analysis
Disulfide bondi41 ↔ 61By similarity
Disulfide bondi67 ↔ 85By similarity
Disulfide bondi86 ↔ 91By similarity
Lipidationi101 – 1011GPI-anchor amidated asparagineBy similarity

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP27274.
PRIDEiP27274.

PTM databases

iPTMnetiP27274.
PhosphoSiteiP27274.
SwissPalmiP27274.

Expressioni

Gene expression databases

GenevisibleiP27274. RN.

Interactioni

GO - Molecular functioni

  • complement binding Source: RGD

Protein-protein interaction databases

IntActiP27274. 1 interaction.
STRINGi10116.ENSRNOP00000060967.

Structurei

3D structure databases

ProteinModelPortaliP27274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 11088UPAR/Ly6Add
BLAST

Sequence similaritiesi

Contains 1 UPAR/Ly6 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J39P. Eukaryota.
ENOG410ZEQP. LUCA.
GeneTreeiENSGT00390000016309.
HOVERGENiHBG005284.
InParanoidiP27274.
KOiK04008.
OMAiNKAAFSI.
OrthoDBiEOG7PVWRQ.
PhylomeDBiP27274.

Family and domain databases

InterProiIPR018363. CD59_antigen_CS.
IPR027101. CD59_glyco.
IPR016054. LY6_UPA_recep-like.
[Graphical view]
PANTHERiPTHR10036:SF9. PTHR10036:SF9. 1 hit.
PfamiPF00021. UPAR_LY6. 1 hit.
[Graphical view]
SMARTiSM00134. LU. 1 hit.
[Graphical view]
PROSITEiPS00983. LY6_UPAR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27274-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRARRGFILL LLLAVLCSTG VSLRCYNCLD PVSSCKTNST CSPNLDACLV
60 70 80 90 100
AVSGKQVYQQ CWRFSDCNAK FILSRLEIAN VQYRCCQADL CNKSFEDKPN
110 120
NGAISLLGKT ALLVTSVLAA ILKPCF
Length:126
Mass (Da):13,790
Last modified:November 1, 1997 - v2
Checksum:i54B9C58AB2073005
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48255 mRNA. Translation: AAA88909.1.
BC063176 mRNA. Translation: AAH63176.1.
PIRiS53340.
RefSeqiNP_037057.1. NM_012925.1.
UniGeneiRn.1231.

Genome annotation databases

EnsembliENSRNOT00000067085; ENSRNOP00000060967; ENSRNOG00000042821.
GeneIDi25407.
KEGGirno:25407.
UCSCiRGD:2311. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48255 mRNA. Translation: AAA88909.1.
BC063176 mRNA. Translation: AAH63176.1.
PIRiS53340.
RefSeqiNP_037057.1. NM_012925.1.
UniGeneiRn.1231.

3D structure databases

ProteinModelPortaliP27274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP27274. 1 interaction.
STRINGi10116.ENSRNOP00000060967.

PTM databases

iPTMnetiP27274.
PhosphoSiteiP27274.
SwissPalmiP27274.

Proteomic databases

PaxDbiP27274.
PRIDEiP27274.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000067085; ENSRNOP00000060967; ENSRNOG00000042821.
GeneIDi25407.
KEGGirno:25407.
UCSCiRGD:2311. rat.

Organism-specific databases

CTDi966.
RGDi2311. Cd59.

Phylogenomic databases

eggNOGiENOG410J39P. Eukaryota.
ENOG410ZEQP. LUCA.
GeneTreeiENSGT00390000016309.
HOVERGENiHBG005284.
InParanoidiP27274.
KOiK04008.
OMAiNKAAFSI.
OrthoDBiEOG7PVWRQ.
PhylomeDBiP27274.

Enzyme and pathway databases

ReactomeiR-RNO-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-RNO-5694530. Cargo concentration in the ER.
R-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-977606. Regulation of Complement cascade.

Miscellaneous databases

PROiP27274.

Gene expression databases

GenevisibleiP27274. RN.

Family and domain databases

InterProiIPR018363. CD59_antigen_CS.
IPR027101. CD59_glyco.
IPR016054. LY6_UPA_recep-like.
[Graphical view]
PANTHERiPTHR10036:SF9. PTHR10036:SF9. 1 hit.
PfamiPF00021. UPAR_LY6. 1 hit.
[Graphical view]
SMARTiSM00134. LU. 1 hit.
[Graphical view]
PROSITEiPS00983. LY6_UPAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the rat analogue of human CD59: structural comparison with human CD59 and identification of a putative active site."
    Rushmere N.K., Harrison R.A., van den Berg C.W., Morgan B.P.
    Biochem. J. 304:595-601(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 23-64.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. "Isolation and characterization of a membrane protein from rat erythrocytes which inhibits lysis by the membrane attack complex of rat complement."
    Hughes T.R., Piddlesden S.J., Willams J.D., Harrison R.A., Morgan B.P.
    Biochem. J. 284:169-176(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-37.
    Tissue: Erythrocyte.

Entry informationi

Entry nameiCD59_RAT
AccessioniPrimary (citable) accession number: P27274
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1997
Last modified: July 6, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.