P27263 (TRAP_TYCSV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional activator protein
Short name=TrAP

Alternative name(s):
Protein C2
Protein L2

Gene names

ORF Names:

C2, L2

Organism

Tomato yellow leaf curl Sardinia virus (TYLCSV) [Complete proteome]

Taxonomic identifier

123735 [NCBI]

Taxonomic lineage

Viruses › ssDNA viruses › Geminiviridae › Begomovirus

Virus host

Cynanchum acutum [TaxID: 185024]
Malva parviflora (Little mallow) (Cheeseweed mallow) [TaxID: 145753]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]

Protein attributes

Sequence length	135 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Strong activator of the late viral genes promoters. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. TrAP suppresses the host RNA silencing by inhibiting adenosine kinase 2 (ADK2), a kinase involved in a general methylation pathway. Also suppresses the host basal defense by interacting with and inhibiting SNF1 kinase, a key regulator of cell metabolism implicated in innate antiviral defense. Determines pathogenicity By similarity.
Subunit structure	Monomer. Homodimer. Homooligomer. Self-interaction correlates with nuclear localization and efficient activation of transcription. Monomers suppress local silencing by interacting with and inactivating host adenosine kinase 2 (ADK2) in the cytoplasm. Interacts with and inhibits host SNF1 kinase. Binds to ssDNA By similarity.
Subcellular location	Host nucleus By similarity. Host cytoplasm By similarity. Note: The phosphorylated form appears to accumulate almost exclusively in the nucleus, whereas the non-phosphorylated form is found in both nucleus and cytoplasm By similarity.
Domain	The zinc finger and the transactivation region are involved in PTGS suppression By similarity.
Post-translational modification	Phosphorylated By similarity.
Sequence similarities	Belongs to the geminiviridae transcriptional activator protein family.

Ontologies

Keywords
Biological process	Host-virus interaction
Cellular component	Host cytoplasm Host nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Suppressor of RNA silencing
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell viral capsid Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 135	135	Transcriptional activator protein		PRO_0000222234
Regions
	Zinc finger	37 – 54	18	By similarity
	Region	120 – 135	16	Transactivation By similarity
	Motif	17 – 32	16	Nuclear localization signal By similarity
	Compositional bias	29 – 32	4	Poly-Arg

Sequences

	Sequence		Length	Mass (Da)
	P27263 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 3DE9A8DC8918A485	FASTA	135	15,719
	10 20 30 40 50 60 MQSSSPSTSH CSQIPIKIQH HIAKKRQVRR RRVDLDCGCS YYIHLDCINH GFTHRGVHHC 70 80 90 100 110 120 ASSNEWRLYL RDNKSPIFHD NQTQSEPIQQ QIQHTNIPNQ IQPQLEEGTG DSQMFSQLPH 130 LDDLTVSDWS FFKSL

References

[1]	"Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted monopartite geminivirus." Kheyr-Pour A., Bendahmane M., Matzeit V., Accotto G.P., Crespi S., Gronenborn B. Nucleic Acids Res. 19:6763-6769(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61153 Genomic DNA. Translation: CAA43464.1.
PIR	S22591.
RefSeq	NP_620740.1. NC_003828.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	944424.
Family and domain databases
InterPro	IPR000942. Gemini_AL2. [Graphical view]
Pfam	PF01440. Gemini_AL2. 1 hit. [Graphical view]
PRINTS	PR00230. GEMCOATAL2.
ProDom	PD001117. Gemini_AL2. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

TRAP_TYCSV

Accession

Primary (citable) accession number: P27263

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program