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Protein

GTPase ArgK

Gene

argK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds and hydrolyzes GTP (PubMed:18950999). Likely functions as a G-protein chaperone that assists AdoCbl cofactor delivery to the methylmalonyl-CoA mutase (MCM) ScpA and reactivation of the enzyme during catalysis.Curated1 Publication

Catalytic activityi

GTP + H2O = GDP + phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi61 – 68ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • GTPase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11445-MONOMER.
ECOL316407:JW2885-MONOMER.
MetaCyc:EG11445-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase ArgK1 Publication (EC:3.6.5.-1 Publication)
Alternative name(s):
G-protein chaperoneCurated
Gene namesi
Name:argK
Synonyms:ygfD
Ordered Locus Names:b2918, JW2885
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11445. argK.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001578161 – 331GTPase ArgKAdd BLAST331

Proteomic databases

PaxDbiP27254.
PRIDEiP27254.

Interactioni

Subunit structurei

Monomer. Interacts with the methylmalonyl-CoA mutase ScpA.1 Publication

Protein-protein interaction databases

BioGridi4259325. 10 interactors.
IntActiP27254. 13 interactors.
STRINGi511145.b2918.

Structurei

Secondary structure

1331
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni4 – 6Combined sources3
Helixi7 – 15Combined sources9
Helixi19 – 30Combined sources12
Helixi34 – 47Combined sources14
Helixi48 – 50Combined sources3
Beta strandi54 – 61Combined sources8
Helixi67 – 80Combined sources14
Beta strandi85 – 90Combined sources6
Turni110 – 113Combined sources4
Beta strandi117 – 122Combined sources6
Helixi131 – 144Combined sources14
Beta strandi148 – 155Combined sources8
Helixi160 – 165Combined sources6
Beta strandi169 – 175Combined sources7
Helixi188 – 193Combined sources6
Beta strandi195 – 199Combined sources5
Helixi207 – 223Combined sources17
Beta strandi234 – 237Combined sources4
Turni240 – 243Combined sources4
Helixi246 – 262Combined sources17
Helixi265 – 292Combined sources28
Helixi294 – 308Combined sources15
Helixi314 – 326Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P67X-ray1.80A2-331[»]
ProteinModelPortaliP27254.
SMRiP27254.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27254.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DJT. Bacteria.
COG1703. LUCA.
HOGENOMiHOG000003902.
InParanoidiP27254.
KOiK07588.
OMAiNKADGPH.
PhylomeDBiP27254.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR005129. GTPase_ArgK.
IPR003661. HisK_dim/P.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00750. lao. 1 hit.

Sequencei

Sequence statusi: Complete.

P27254-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINEATLAES IRRLRQGERA TLAQAMTLVE SRHPRHQALS TQLLDAIMPY
60 70 80 90 100
CGNTLRLGVT GTPGAGKSTF LEAFGMLLIR EGLKVAVIAV DPSSPVTGGS
110 120 130 140 150
ILGDKTRMND LARAEAAFIR PVPSSGHLGG ASQRARELML LCEAAGYDVV
160 170 180 190 200
IVETVGVGQS ETEVARMVDC FISLQIAGGG DDLQGIKKGL MEVADLIVIN
210 220 230 240 250
KDDGDNHTNV AIARHMYESA LHILRRKYDE WQPRVLTCSA LEKRGIDEIW
260 270 280 290 300
HAIIDFKTAL TASGRLQQVR QQQSVEWLRK QTEEEVLNHL FANEDFDRYY
310 320 330
RQTLLAVKNN TLSPRTGLRQ LSEFIQTQYF D
Length:331
Mass (Da):36,704
Last modified:October 1, 1996 - v2
Checksum:i02C27AD2000C6137
GO

Sequence cautioni

The sequence CAA47312 differs from that shown. Reason: Frameshift at several positions.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti68S → R in strain: RC101. 1
Natural varianti154T → A in strain: RC101. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66836 Genomic DNA. Translation: CAA47312.1. Frameshift.
U28377 Genomic DNA. Translation: AAA69085.1.
U00096 Genomic DNA. Translation: AAC75955.1.
AP009048 Genomic DNA. Translation: BAE76982.1.
PIRiE65076.
RefSeqiNP_417393.1. NC_000913.3.
WP_000606525.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75955; AAC75955; b2918.
BAE76982; BAE76982; BAE76982.
GeneIDi947412.
KEGGiecj:JW2885.
eco:b2918.
PATRICi32121250. VBIEscCol129921_3013.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66836 Genomic DNA. Translation: CAA47312.1. Frameshift.
U28377 Genomic DNA. Translation: AAA69085.1.
U00096 Genomic DNA. Translation: AAC75955.1.
AP009048 Genomic DNA. Translation: BAE76982.1.
PIRiE65076.
RefSeqiNP_417393.1. NC_000913.3.
WP_000606525.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P67X-ray1.80A2-331[»]
ProteinModelPortaliP27254.
SMRiP27254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259325. 10 interactors.
IntActiP27254. 13 interactors.
STRINGi511145.b2918.

Proteomic databases

PaxDbiP27254.
PRIDEiP27254.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75955; AAC75955; b2918.
BAE76982; BAE76982; BAE76982.
GeneIDi947412.
KEGGiecj:JW2885.
eco:b2918.
PATRICi32121250. VBIEscCol129921_3013.

Organism-specific databases

EchoBASEiEB1415.
EcoGeneiEG11445. argK.

Phylogenomic databases

eggNOGiENOG4105DJT. Bacteria.
COG1703. LUCA.
HOGENOMiHOG000003902.
InParanoidiP27254.
KOiK07588.
OMAiNKADGPH.
PhylomeDBiP27254.

Enzyme and pathway databases

BioCyciEcoCyc:EG11445-MONOMER.
ECOL316407:JW2885-MONOMER.
MetaCyc:EG11445-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP27254.
PROiP27254.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR005129. GTPase_ArgK.
IPR003661. HisK_dim/P.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00750. lao. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARGK_ECOLI
AccessioniPrimary (citable) accession number: P27254
Secondary accession number(s): Q2M9S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Part of an operon that encodes enzymes converting succinate to propionate.1 Publication

Caution

Was originally thought to be a membrane protein kinase involved in phosphorylation of the AO and LAO periplasmic-binding proteins (PubMed:9733684). However, its role needs to be reexamined.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.