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Protein

Methylmalonyl-CoA mutase

Gene

scpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA. Could be part of a pathway that converts succinate to propionate.2 Publications

Catalytic activityi

(R)-methylmalonyl-CoA = succinyl-CoA.2 Publications

Cofactori

adenosylcob(III)alamin2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi597Cobalt (cobalamin axial ligand)By similarity1

GO - Molecular functioni

  • cobalamin binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • methylmalonyl-CoA mutase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionIsomerase
LigandCobalamin, Cobalt, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:METHYLMALONYL-COA-MUT-MONOMER
MetaCyc:METHYLMALONYL-COA-MUT-MONOMER

Chemistry databases

SwissLipidsiSLP:000001257

Names & Taxonomyi

Protein namesi
Recommended name:
Methylmalonyl-CoA mutase (EC:5.4.99.2)
Short name:
MCM
Gene namesi
Name:scpA
Synonyms:sbm, yliK
Ordered Locus Names:b2917, JW2884
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11444 scpA

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001942771 – 714Methylmalonyl-CoA mutaseAdd BLAST714

Proteomic databases

PaxDbiP27253
PRIDEiP27253

Interactioni

Subunit structurei

Homodimer. Interacts with ArgK.1 Publication

Protein-protein interaction databases

BioGridi4262330, 27 interactors
IntActiP27253, 6 interactors
STRINGi316407.85675728

Structurei

3D structure databases

ProteinModelPortaliP27253
SMRiP27253
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini584 – 714B12-bindingPROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Belongs to the methylmalonyl-CoA mutase family.Curated

Phylogenomic databases

eggNOGiENOG4105D5P Bacteria
COG1884 LUCA
COG2185 LUCA
HOGENOMiHOG000003917
InParanoidiP27253
KOiK01847
OMAiPLFQTPR
PhylomeDBiP27253

Family and domain databases

InterProiView protein in InterPro
IPR006159 Acid_CoA_mut_C
IPR016176 Cbl-dep_enz_cat
IPR006158 Cobalamin-bd
IPR036724 Cobalamin-bd_sf
IPR006099 MeMalonylCoA_mutase_a/b_cat
IPR006098 MMCoA_mutase_a_cat
PfamiView protein in Pfam
PF02310 B12-binding, 1 hit
PF01642 MM_CoA_mutase, 1 hit
SUPFAMiSSF51703 SSF51703, 1 hit
SSF52242 SSF52242, 1 hit
TIGRFAMsiTIGR00640 acid_CoA_mut_C, 1 hit
TIGR00641 acid_CoA_mut_N, 1 hit
PROSITEiView protein in PROSITE
PS51332 B12_BINDING, 1 hit
PS00544 METMALONYL_COA_MUTASE, 1 hit

Sequencei

Sequence statusi: Complete.

P27253-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNVQEWQQL ANKELSRREK TVDSLVHQTA EGIAIKPLYT EADLDNLEVT
60 70 80 90 100
GTLPGLPPYV RGPRATMYTA QPWTIRQYAG FSTAKESNAF YRRNLAAGQK
110 120 130 140 150
GLSVAFDLAT HRGYDSDNPR VAGDVGKAGV AIDTVEDMKV LFDQIPLDKM
160 170 180 190 200
SVSMTMNGAV LPVLAFYIVA AEEQGVTPDK LTGTIQNDIL KEYLCRNTYI
210 220 230 240 250
YPPKPSMRII ADIIAWCSGN MPRFNTISIS GYHMGEAGAN CVQQVAFTLA
260 270 280 290 300
DGIEYIKAAI SAGLKIDDFA PRLSFFFGIG MDLFMNVAML RAARYLWSEA
310 320 330 340 350
VSGFGAQDPK SLALRTHCQT SGWSLTEQDP YNNVIRTTIE ALAATLGGTQ
360 370 380 390 400
SLHTNAFDEA LGLPTDFSAR IARNTQIIIQ EESELCRTVD PLAGSYYIES
410 420 430 440 450
LTDQIVKQAR AIIQQIDEAG GMAKAIEAGL PKRMIEEASA REQSLIDQGK
460 470 480 490 500
RVIVGVNKYK LDHEDETDVL EIDNVMVRNE QIASLERIRA TRDDAAVTAA
510 520 530 540 550
LNALTHAAQH NENLLAAAVN AARVRATLGE ISDALEVAFD RYLVPSQCVT
560 570 580 590 600
GVIAQSYHQS EKSASEFDAI VAQTEQFLAD NGRRPRILIA KMGQDGHDRG
610 620 630 640 650
AKVIASAYSD LGFDVDLSPM FSTPEEIARL AVENDVHVVG ASSLAAGHKT
660 670 680 690 700
LIPELVEALK KWGREDICVV AGGVIPPQDY AFLQERGVAA IYGPGTPMLD
710
SVRDVLNLIS QHHD
Length:714
Mass (Da):77,871
Last modified:August 29, 2003 - v2
Checksum:i65222F5EB2F4633A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti356A → V in CAA47311 (PubMed:1355087).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66836 Genomic DNA Translation: CAA47311.1
U28377 Genomic DNA Translation: AAA69084.1
U00096 Genomic DNA Translation: AAC75954.1
AP009048 Genomic DNA Translation: BAE76981.1
PIRiD65076
RefSeqiNP_417392.1, NC_000913.3
WP_000073223.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75954; AAC75954; b2917
BAE76981; BAE76981; BAE76981
GeneIDi945576
KEGGiecj:JW2884
eco:b2917
PATRICifig|1411691.4.peg.3815

Similar proteinsi

Entry informationi

Entry nameiSCPA_ECOLI
AccessioniPrimary (citable) accession number: P27253
Secondary accession number(s): Q2M9S5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 29, 2003
Last modified: March 28, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
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