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Protein

Methylmalonyl-CoA mutase

Gene

scpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA. Could be part of a pathway that converts succinate to propionate.2 Publications

Catalytic activityi

(R)-methylmalonyl-CoA = succinyl-CoA.2 Publications

Cofactori

adenosylcob(III)alamin2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi597Cobalt (cobalamin axial ligand)By similarity1

GO - Molecular functioni

  • cobalamin binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • methylmalonyl-CoA mutase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Cobalamin, Cobalt, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:METHYLMALONYL-COA-MUT-MONOMER.
ECOL316407:JW2884-MONOMER.
MetaCyc:METHYLMALONYL-COA-MUT-MONOMER.

Chemistry databases

SwissLipidsiSLP:000001257.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylmalonyl-CoA mutase (EC:5.4.99.2)
Short name:
MCM
Gene namesi
Name:scpA
Synonyms:sbm, yliK
Ordered Locus Names:b2917, JW2884
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11444. scpA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001942771 – 714Methylmalonyl-CoA mutaseAdd BLAST714

Proteomic databases

PaxDbiP27253.
PRIDEiP27253.

Interactioni

Subunit structurei

Homodimer. Interacts with ArgK.1 Publication

Protein-protein interaction databases

BioGridi4262330. 25 interactors.
IntActiP27253. 6 interactors.
STRINGi511145.b2917.

Structurei

3D structure databases

ProteinModelPortaliP27253.
SMRiP27253.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini584 – 714B12-bindingPROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Belongs to the methylmalonyl-CoA mutase family.Curated
Contains 1 B12-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105D5P. Bacteria.
COG1884. LUCA.
COG2185. LUCA.
HOGENOMiHOG000003917.
InParanoidiP27253.
KOiK01847.
OMAiGVNKYRP.
PhylomeDBiP27253.

Family and domain databases

Gene3Di3.20.20.240. 1 hit.
3.40.50.280. 1 hit.
InterProiIPR006159. Acid_CoA_mut_C.
IPR016176. Cbl-dep_enz_cat.
IPR014348. Cbl-dep_enz_cat-sub.
IPR006158. Cobalamin-bd.
IPR006099. MeMalonylCoA_mutase_a/b_cat.
IPR006098. MMCoA_mutase_a_cat.
[Graphical view]
PfamiPF02310. B12-binding. 1 hit.
PF01642. MM_CoA_mutase. 1 hit.
[Graphical view]
SUPFAMiSSF51703. SSF51703. 1 hit.
SSF52242. SSF52242. 1 hit.
TIGRFAMsiTIGR00640. acid_CoA_mut_C. 1 hit.
TIGR00641. acid_CoA_mut_N. 1 hit.
PROSITEiPS51332. B12_BINDING. 1 hit.
PS00544. METMALONYL_COA_MUTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27253-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNVQEWQQL ANKELSRREK TVDSLVHQTA EGIAIKPLYT EADLDNLEVT
60 70 80 90 100
GTLPGLPPYV RGPRATMYTA QPWTIRQYAG FSTAKESNAF YRRNLAAGQK
110 120 130 140 150
GLSVAFDLAT HRGYDSDNPR VAGDVGKAGV AIDTVEDMKV LFDQIPLDKM
160 170 180 190 200
SVSMTMNGAV LPVLAFYIVA AEEQGVTPDK LTGTIQNDIL KEYLCRNTYI
210 220 230 240 250
YPPKPSMRII ADIIAWCSGN MPRFNTISIS GYHMGEAGAN CVQQVAFTLA
260 270 280 290 300
DGIEYIKAAI SAGLKIDDFA PRLSFFFGIG MDLFMNVAML RAARYLWSEA
310 320 330 340 350
VSGFGAQDPK SLALRTHCQT SGWSLTEQDP YNNVIRTTIE ALAATLGGTQ
360 370 380 390 400
SLHTNAFDEA LGLPTDFSAR IARNTQIIIQ EESELCRTVD PLAGSYYIES
410 420 430 440 450
LTDQIVKQAR AIIQQIDEAG GMAKAIEAGL PKRMIEEASA REQSLIDQGK
460 470 480 490 500
RVIVGVNKYK LDHEDETDVL EIDNVMVRNE QIASLERIRA TRDDAAVTAA
510 520 530 540 550
LNALTHAAQH NENLLAAAVN AARVRATLGE ISDALEVAFD RYLVPSQCVT
560 570 580 590 600
GVIAQSYHQS EKSASEFDAI VAQTEQFLAD NGRRPRILIA KMGQDGHDRG
610 620 630 640 650
AKVIASAYSD LGFDVDLSPM FSTPEEIARL AVENDVHVVG ASSLAAGHKT
660 670 680 690 700
LIPELVEALK KWGREDICVV AGGVIPPQDY AFLQERGVAA IYGPGTPMLD
710
SVRDVLNLIS QHHD
Length:714
Mass (Da):77,871
Last modified:August 29, 2003 - v2
Checksum:i65222F5EB2F4633A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti356A → V in CAA47311 (PubMed:1355087).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66836 Genomic DNA. Translation: CAA47311.1.
U28377 Genomic DNA. Translation: AAA69084.1.
U00096 Genomic DNA. Translation: AAC75954.1.
AP009048 Genomic DNA. Translation: BAE76981.1.
PIRiD65076.
RefSeqiNP_417392.1. NC_000913.3.
WP_000073223.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75954; AAC75954; b2917.
BAE76981; BAE76981; BAE76981.
GeneIDi945576.
KEGGiecj:JW2884.
eco:b2917.
PATRICi32121248. VBIEscCol129921_3012.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66836 Genomic DNA. Translation: CAA47311.1.
U28377 Genomic DNA. Translation: AAA69084.1.
U00096 Genomic DNA. Translation: AAC75954.1.
AP009048 Genomic DNA. Translation: BAE76981.1.
PIRiD65076.
RefSeqiNP_417392.1. NC_000913.3.
WP_000073223.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP27253.
SMRiP27253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262330. 25 interactors.
IntActiP27253. 6 interactors.
STRINGi511145.b2917.

Chemistry databases

SwissLipidsiSLP:000001257.

Proteomic databases

PaxDbiP27253.
PRIDEiP27253.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75954; AAC75954; b2917.
BAE76981; BAE76981; BAE76981.
GeneIDi945576.
KEGGiecj:JW2884.
eco:b2917.
PATRICi32121248. VBIEscCol129921_3012.

Organism-specific databases

EchoBASEiEB1414.
EcoGeneiEG11444. scpA.

Phylogenomic databases

eggNOGiENOG4105D5P. Bacteria.
COG1884. LUCA.
COG2185. LUCA.
HOGENOMiHOG000003917.
InParanoidiP27253.
KOiK01847.
OMAiGVNKYRP.
PhylomeDBiP27253.

Enzyme and pathway databases

BioCyciEcoCyc:METHYLMALONYL-COA-MUT-MONOMER.
ECOL316407:JW2884-MONOMER.
MetaCyc:METHYLMALONYL-COA-MUT-MONOMER.

Miscellaneous databases

PROiP27253.

Family and domain databases

Gene3Di3.20.20.240. 1 hit.
3.40.50.280. 1 hit.
InterProiIPR006159. Acid_CoA_mut_C.
IPR016176. Cbl-dep_enz_cat.
IPR014348. Cbl-dep_enz_cat-sub.
IPR006158. Cobalamin-bd.
IPR006099. MeMalonylCoA_mutase_a/b_cat.
IPR006098. MMCoA_mutase_a_cat.
[Graphical view]
PfamiPF02310. B12-binding. 1 hit.
PF01642. MM_CoA_mutase. 1 hit.
[Graphical view]
SUPFAMiSSF51703. SSF51703. 1 hit.
SSF52242. SSF52242. 1 hit.
TIGRFAMsiTIGR00640. acid_CoA_mut_C. 1 hit.
TIGR00641. acid_CoA_mut_N. 1 hit.
PROSITEiPS51332. B12_BINDING. 1 hit.
PS00544. METMALONYL_COA_MUTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCPA_ECOLI
AccessioniPrimary (citable) accession number: P27253
Secondary accession number(s): Q2M9S5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 29, 2003
Last modified: November 2, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.