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Reviewed, UniProtKB/Swiss-Prot P27248 (GCST_ECOLI)

Last modified February 9, 2010. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Aminomethyltransferase
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein
Gene names
Name: gcvT
Ordered Locus Names: b2905, JW2873
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length364 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

The glycine cleavage system catalyzes the degradation of glycine. HAMAP MF_00259

Catalytic activity

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259

Subunit structure

The glycine cleavage system is composed of four proteins: P, T, L and H. HAMAP MF_00259

Sequence similarities

Belongs to the gcvT family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2 Ref.5
Chain2 – 364363Aminomethyltransferase HAMAP MF_00259
PRO_0000122554

Secondary structure

1 ..................................................................... 364
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P27248-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 1F58C5A244F82242

FASTA36440,147
        10         20         30         40         50         60 
MAQQTPLYEQ HTLCGARMVD FHGWMMPLHY GSQIDEHHAV RTDAGMFDVS HMTIVDLRGS 

        70         80         90        100        110        120 
RTREFLRYLL ANDVAKLTKS GKALYSGMLN ASGGVIDDLI VYYFTEDFFR LVVNSATREK 

       130        140        150        160        170        180 
DLSWITQHAE PFGIEITVRD DLSMIAVQGP NAQAKAATLF NDAQRQAVEG MKPFFGVQAG 

       190        200        210        220        230        240 
DLFIATTGYT GEAGYEIALP NEKAADFWRA LVEAGVKPCG LGARDTLRLE AGMNLYGQEM 

       250        260        270        280        290        300 
DETISPLAAN MGWTIAWEPA DRDFIGREAL EVQREHGTEK LVGLVMTEKG VLRNELPVRF 

       310        320        330        340        350        360 
TDAQGNQHEG IITSGTFSPT LGYSIALARV PEGIGETAIV QIRNREMPVK VTKPVFVRNG 


KAVA

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References

« Hide 'large scale' references
[1]"The Escherichia coli gcvT gene encoding the T-protein of the glycine cleavage enzyme system."
Stauffer L.T., Ghrist A., Stauffer G.V.
DNA Seq. 3:339-346(1993) [PubMed: 8219277] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system."
Okamura-Ikeda K., Ohmura Y., Fujiwara K., Motokawa Y.
Eur. J. Biochem. 216:539-548(1993) [PubMed: 8375392] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-13.
Strain: K12 / EMG2.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M97263 Unassigned DNA. Translation: AAC36843.1.
X73958 Genomic DNA. Translation: CAA52144.1.
U28377 Genomic DNA. Translation: AAA69073.1.
U00096 Genomic DNA. Translation: AAC75943.1.
AP009048 Genomic DNA. Translation: BAE76970.1.
PIRA56689.
RefSeqAP_003464.1.
NP_417381.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VLOX-ray1.70A2-363[»]
ModBaseSearch...

Protein-protein interaction databases

STRINGP27248.

Genome annotation databases

GeneID947390.
GenomeReviewsGene locus JW2873 in contig AP009048_GR.
Gene locus b2905 in contig U00096_GR.
KEGGecj:JW2873.
eco:b2905.

Organism-specific databases

EchoBASEEB1412.
EcoGeneEG11442. gcvT.
CMRSearch...

Phylogenomic databases

eggNOGCOG0404.
HOGENOMHBG299834.
OMAKALYGGM.

Enzyme and pathway databases

BioCycEcoCyc:GCVT-MONOMER.
ECOL168927:B2905-MONOMER.
MetaCyc:GCVT-MONOMER.

Gene expression databases

GenevestigatorP27248.

Family and domain databases

HAMAPMF_00259. GcvT.
[Tree]
InterProIPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR006223. GcvT.
[Graphical view]
PfamPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFPIRSF006487. GcvT. 1 hit.
TIGRFAMsTIGR00528. gcvT. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameGCST_ECOLI
AccessionPrimary (citable) accession number: P27248
Secondary accession number(s): Q2M9T6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: February 9, 2010
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents