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Protein

Aminomethyltransferase

Gene

gcvT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine.

Catalytic activityi

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.

GO - Molecular functioni

  • aminomethyltransferase activity Source: EcoCyc
  • transaminase activity Source: UniProtKB-KW

GO - Biological processi

  • glycine decarboxylation via glycine cleavage system Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:GCVT-MONOMER.
ECOL316407:JW2873-MONOMER.
MetaCyc:GCVT-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminomethyltransferase (EC:2.1.2.10)
Alternative name(s):
Glycine cleavage system T protein
Gene namesi
Name:gcvT
Ordered Locus Names:b2905, JW2873
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11442. gcvT.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001225542 – 364AminomethyltransferaseAdd BLAST363

Proteomic databases

EPDiP27248.
PaxDbiP27248.
PRIDEiP27248.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.

Protein-protein interaction databases

BioGridi4262343. 12 interactors.
IntActiP27248. 6 interactors.
STRINGi511145.b2905.

Structurei

Secondary structure

1364
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 13Combined sources6
Beta strandi17 – 21Combined sources5
Beta strandi24 – 31Combined sources8
Helixi33 – 42Combined sources10
Beta strandi45 – 48Combined sources4
Beta strandi52 – 59Combined sources8
Helixi62 – 69Combined sources8
Beta strandi70 – 72Combined sources3
Helixi74 – 76Combined sources3
Beta strandi82 – 89Combined sources8
Beta strandi95 – 105Combined sources11
Beta strandi108 – 113Combined sources6
Helixi115 – 117Combined sources3
Helixi118 – 129Combined sources12
Helixi130 – 132Combined sources3
Beta strandi135 – 138Combined sources4
Beta strandi142 – 149Combined sources8
Helixi152 – 157Combined sources6
Helixi162 – 168Combined sources7
Beta strandi173 – 179Combined sources7
Beta strandi182 – 185Combined sources4
Beta strandi189 – 192Combined sources4
Beta strandi194 – 200Combined sources7
Helixi201 – 213Combined sources13
Helixi221 – 230Combined sources10
Turni236 – 238Combined sources3
Helixi246 – 248Combined sources3
Helixi252 – 254Combined sources3
Helixi267 – 276Combined sources10
Beta strandi280 – 286Combined sources7
Beta strandi288 – 290Combined sources3
Beta strandi297 – 301Combined sources5
Beta strandi307 – 318Combined sources12
Turni319 – 322Combined sources4
Beta strandi323 – 330Combined sources8
Beta strandi336 – 342Combined sources7
Beta strandi345 – 352Combined sources8
Beta strandi356 – 358Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VLOX-ray1.70A2-363[»]
3A8IX-ray1.99A/B/C/D1-364[»]
3A8JX-ray1.98A/B/C/D1-364[»]
3A8KX-ray1.95A/B/C/D1-364[»]
ProteinModelPortaliP27248.
SMRiP27248.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27248.

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.Curated

Phylogenomic databases

eggNOGiENOG4105D43. Bacteria.
COG0404. LUCA.
HOGENOMiHOG000239381.
InParanoidiP27248.
KOiK00605.
OMAiLGWLVHL.
PhylomeDBiP27248.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
HAMAPiMF_00259. GcvT. 1 hit.
InterProiIPR006223. GCS_T.
IPR022903. GCS_T_bac.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PANTHERiPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQQTPLYEQ HTLCGARMVD FHGWMMPLHY GSQIDEHHAV RTDAGMFDVS
60 70 80 90 100
HMTIVDLRGS RTREFLRYLL ANDVAKLTKS GKALYSGMLN ASGGVIDDLI
110 120 130 140 150
VYYFTEDFFR LVVNSATREK DLSWITQHAE PFGIEITVRD DLSMIAVQGP
160 170 180 190 200
NAQAKAATLF NDAQRQAVEG MKPFFGVQAG DLFIATTGYT GEAGYEIALP
210 220 230 240 250
NEKAADFWRA LVEAGVKPCG LGARDTLRLE AGMNLYGQEM DETISPLAAN
260 270 280 290 300
MGWTIAWEPA DRDFIGREAL EVQREHGTEK LVGLVMTEKG VLRNELPVRF
310 320 330 340 350
TDAQGNQHEG IITSGTFSPT LGYSIALARV PEGIGETAIV QIRNREMPVK
360
VTKPVFVRNG KAVA
Length:364
Mass (Da):40,147
Last modified:January 23, 2007 - v3
Checksum:i1F58C5A244F82242
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97263 Unassigned DNA. Translation: AAC36843.1.
X73958 Genomic DNA. Translation: CAA52144.1.
U28377 Genomic DNA. Translation: AAA69073.1.
U00096 Genomic DNA. Translation: AAC75943.1.
AP009048 Genomic DNA. Translation: BAE76970.1.
PIRiA56689.
RefSeqiNP_417381.1. NC_000913.3.
WP_000068701.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75943; AAC75943; b2905.
BAE76970; BAE76970; BAE76970.
GeneIDi947390.
KEGGiecj:JW2873.
eco:b2905.
PATRICi32121224. VBIEscCol129921_3000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97263 Unassigned DNA. Translation: AAC36843.1.
X73958 Genomic DNA. Translation: CAA52144.1.
U28377 Genomic DNA. Translation: AAA69073.1.
U00096 Genomic DNA. Translation: AAC75943.1.
AP009048 Genomic DNA. Translation: BAE76970.1.
PIRiA56689.
RefSeqiNP_417381.1. NC_000913.3.
WP_000068701.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VLOX-ray1.70A2-363[»]
3A8IX-ray1.99A/B/C/D1-364[»]
3A8JX-ray1.98A/B/C/D1-364[»]
3A8KX-ray1.95A/B/C/D1-364[»]
ProteinModelPortaliP27248.
SMRiP27248.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262343. 12 interactors.
IntActiP27248. 6 interactors.
STRINGi511145.b2905.

Proteomic databases

EPDiP27248.
PaxDbiP27248.
PRIDEiP27248.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75943; AAC75943; b2905.
BAE76970; BAE76970; BAE76970.
GeneIDi947390.
KEGGiecj:JW2873.
eco:b2905.
PATRICi32121224. VBIEscCol129921_3000.

Organism-specific databases

EchoBASEiEB1412.
EcoGeneiEG11442. gcvT.

Phylogenomic databases

eggNOGiENOG4105D43. Bacteria.
COG0404. LUCA.
HOGENOMiHOG000239381.
InParanoidiP27248.
KOiK00605.
OMAiLGWLVHL.
PhylomeDBiP27248.

Enzyme and pathway databases

BioCyciEcoCyc:GCVT-MONOMER.
ECOL316407:JW2873-MONOMER.
MetaCyc:GCVT-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP27248.
PROiP27248.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
HAMAPiMF_00259. GcvT. 1 hit.
InterProiIPR006223. GCS_T.
IPR022903. GCS_T_bac.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PANTHERiPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGCST_ECOLI
AccessioniPrimary (citable) accession number: P27248
Secondary accession number(s): Q2M9T6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.