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Protein

Phosphate acyltransferase

Gene

plsX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible formation of acyl-phosphate (acyl-PO4) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.UniRule annotation1 Publication

Catalytic activityi

Acyl-[acyl-carrier-protein] + phosphate = acyl-phosphate + [acyl-carrier-protein].UniRule annotation

Pathwayi: phospholipid metabolism

This protein is involved in the pathway phospholipid metabolism, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway phospholipid metabolism and in Lipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • fatty acid biosynthetic process Source: UniProtKB-HAMAP
  • phospholipid biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:EG11437-MONOMER.
ECOL316407:JW5156-MONOMER.
UniPathwayiUPA00085.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate acyltransferaseUniRule annotation (EC:2.3.1.n2UniRule annotation)
Alternative name(s):
Acyl-ACP phosphotransacylaseUniRule annotation
Acyl-[acyl-carrier-protein]--phosphate acyltransferaseUniRule annotation
Phosphate-acyl-ACP acyltransferaseUniRule annotation
Gene namesi
Name:plsXUniRule annotation
Ordered Locus Names:b1090, JW5156
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11437. plsX.

Subcellular locationi

  • Cytoplasm

  • Note: Associated with the membrane possibly through PlsY.Curated

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Phosphate acyltransferasePRO_0000189875Add
BLAST

Proteomic databases

PaxDbiP27247.

Interactioni

Subunit structurei

Homodimer. Probably interacts with PlsY.UniRule annotation

Protein-protein interaction databases

BioGridi4261030. 142 interactions.
DIPiDIP-10517N.
IntActiP27247. 1 interaction.
STRINGi511145.b1090.

Structurei

3D structure databases

ProteinModelPortaliP27247.
SMRiP27247. Positions 4-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PlsX family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6B. Bacteria.
COG0416. LUCA.
HOGENOMiHOG000154730.
InParanoidiP27247.
KOiK03621.
OMAiKFCLRTM.
OrthoDBiEOG68H88P.
PhylomeDBiP27247.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00019. PlsX.
InterProiIPR003664. FA_synthesis.
IPR024084. IsoPropMal-DH-like_dom.
IPR012281. Phospholipid_synth_PlsX-like.
[Graphical view]
PfamiPF02504. FA_synthesis. 1 hit.
[Graphical view]
PIRSFiPIRSF002465. Phsphlp_syn_PlsX. 1 hit.
TIGRFAMsiTIGR00182. plsX. 1 hit.

Sequencei

Sequence statusi: Complete.

P27247-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRLTLALDV MGGDFGPSVT VPAALQALNS NSQLTLLLVG NSDAITPLLA
60 70 80 90 100
KADFEQRSRL QIIPAQSVIA SDARPSQAIR ASRGSSMRVA LELVKEGRAQ
110 120 130 140 150
ACVSAGNTGA LMGLAKLLLK PLEGIERPAL VTVLPHQQKG KTVVLDLGAN
160 170 180 190 200
VDCDSTMLVQ FAIMGSVLAE EVVEIPNPRV ALLNIGEEEV KGLDSIRDAS
210 220 230 240 250
AVLKTIPSIN YIGYLEANEL LTGKTDVLVC DGFTGNVTLK TMEGVVRMFL
260 270 280 290 300
SLLKSQGEGK KRSWWLLLLK RWLQKSLTRR FSHLNPDQYN GACLLGLRGT
310 320 330 340 350
VIKSHGAANQ RAFAVAIEQA VQAVQRQVPQ RIAARLESVY PAGFELLDGG

KSGTLR
Length:356
Mass (Da):38,214
Last modified:May 30, 2000 - v2
Checksum:i89C6C64C57184DF4
GO

Sequence cautioni

The sequence AAB59064.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96793 Genomic DNA. Translation: AAB59064.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74174.2.
AP009048 Genomic DNA. Translation: BAA35898.2.
PIRiG64852.
RefSeqiNP_415608.2. NC_000913.3.
WP_014639043.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74174; AAC74174; b1090.
BAA35898; BAA35898; BAA35898.
GeneIDi946165.
KEGGiecj:JW5156.
eco:b1090.
PATRICi32117421. VBIEscCol129921_1133.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96793 Genomic DNA. Translation: AAB59064.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74174.2.
AP009048 Genomic DNA. Translation: BAA35898.2.
PIRiG64852.
RefSeqiNP_415608.2. NC_000913.3.
WP_014639043.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP27247.
SMRiP27247. Positions 4-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261030. 142 interactions.
DIPiDIP-10517N.
IntActiP27247. 1 interaction.
STRINGi511145.b1090.

Proteomic databases

PaxDbiP27247.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74174; AAC74174; b1090.
BAA35898; BAA35898; BAA35898.
GeneIDi946165.
KEGGiecj:JW5156.
eco:b1090.
PATRICi32117421. VBIEscCol129921_1133.

Organism-specific databases

EchoBASEiEB1407.
EcoGeneiEG11437. plsX.

Phylogenomic databases

eggNOGiENOG4105C6B. Bacteria.
COG0416. LUCA.
HOGENOMiHOG000154730.
InParanoidiP27247.
KOiK03621.
OMAiKFCLRTM.
OrthoDBiEOG68H88P.
PhylomeDBiP27247.

Enzyme and pathway databases

UniPathwayiUPA00085.
BioCyciEcoCyc:EG11437-MONOMER.
ECOL316407:JW5156-MONOMER.

Miscellaneous databases

PROiP27247.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00019. PlsX.
InterProiIPR003664. FA_synthesis.
IPR024084. IsoPropMal-DH-like_dom.
IPR012281. Phospholipid_synth_PlsX-like.
[Graphical view]
PfamiPF02504. FA_synthesis. 1 hit.
[Graphical view]
PIRSFiPIRSF002465. Phsphlp_syn_PlsX. 1 hit.
TIGRFAMsiTIGR00182. plsX. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Physical locations of genes in the rne (ams)-rpmF-plsX-fab region of the Escherichia coli K-12 chromosome."
    Oh W., Larson T.J.
    J. Bacteriol. 174:7873-7874(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-30.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli."
    Yoshimura M., Oshima T., Ogasawara N.
    BMC Microbiol. 7:69-69(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PHOSPHOLIPID BIOSYNTHESIS.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPLSX_ECOLI
AccessioniPrimary (citable) accession number: P27247
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: May 30, 2000
Last modified: January 20, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.