Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P27245

- MARR_ECOLI

UniProt

P27245 - MARR_ECOLI

Protein

Multiple antibiotic resistance protein MarR

Gene

marR

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Repressor of the marRAB operon which is involved in the activation of both antibiotic resistance and oxidative stress genes. Binds to the marO operator/promoter site.

    GO - Molecular functioni

    1. DNA binding Source: EcoCyc
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. cellular response to antibiotic Source: EcoCyc
    2. negative regulation of transcription, DNA-templated Source: EcoCyc
    3. response to heat Source: EcoCyc
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Antibiotic resistance, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:PD00364.
    ECOL316407:JW5248-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multiple antibiotic resistance protein MarR
    Gene namesi
    Name:marR
    Synonyms:cfxB, inaR, soxQ
    Ordered Locus Names:b1530, JW5248
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11435. marR.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi45 – 451V → E: Increased transcription of the region II transcript. 1 Publication
    Mutagenesisi77 – 771R → L: Increased transcription of the region II transcript. 1 Publication
    Mutagenesisi123 – 14422Missing: Increased transcription of the region II transcript. 1 PublicationAdd
    BLAST

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 144144Multiple antibiotic resistance protein MarRPRO_0000054362Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP27245.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    gyrAP0AES42EBI-6409744,EBI-547129

    Protein-protein interaction databases

    DIPiDIP-10164N.
    IntActiP27245. 1 interaction.
    STRINGi511145.b1530.

    Structurei

    Secondary structure

    1
    144
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi14 – 3320
    Turni34 – 374
    Helixi40 – 5213
    Beta strandi53 – 553
    Helixi57 – 648
    Helixi68 – 8013
    Beta strandi83 – 886
    Turni90 – 923
    Beta strandi96 – 1005
    Helixi102 – 12423
    Turni125 – 1306
    Helixi132 – 1409

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JGSX-ray2.30A7-144[»]
    3VB2X-ray2.60A/B1-144[»]
    3VODX-ray2.60A/B1-144[»]
    3VOEX-ray2.60A/B1-144[»]
    4JBAX-ray2.50A/B1-144[»]
    ProteinModelPortaliP27245.
    SMRiP27245. Positions 7-144.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP27245.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 144134HTH marR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HTH marR-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1846.
    HOGENOMiHOG000221449.
    KOiK03712.
    OrthoDBiEOG6PGKBH.
    PhylomeDBiP27245.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR000835. HTH_MarR-typ.
    IPR023187. Tscrpt_reg_MarR-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF01047. MarR. 1 hit.
    [Graphical view]
    PRINTSiPR00598. HTHMARR.
    SMARTiSM00347. HTH_MARR. 1 hit.
    [Graphical view]
    PROSITEiPS01117. HTH_MARR_1. 1 hit.
    PS50995. HTH_MARR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27245-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSTSDLFNE IIPLGRLIHM VNQKKDRLLN EYLSPLDITA AQFKVLCSIR    50
    CAACITPVEL KKVLSVDLGA LTRMLDRLVC KGWVERLPNP NDKRGVLVKL 100
    TTGGAAICEQ CHQLVGQDLH QELTKNLTAD EVATLEYLLK KVLP 144
    Length:144
    Mass (Da):16,065
    Last modified:December 1, 2000 - v2
    Checksum:iBE7DF5549E24D1D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96235 Genomic DNA. Translation: AAC16394.2.
    U00096 Genomic DNA. Translation: AAC74603.2.
    AP009048 Genomic DNA. Translation: BAA15220.1.
    PIRiE64907.
    RefSeqiNP_416047.4. NC_000913.3.
    YP_489793.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74603; AAC74603; b1530.
    BAA15220; BAA15220; BAA15220.
    GeneIDi12930666.
    945825.
    KEGGiecj:Y75_p1505.
    eco:b1530.
    PATRICi32118358. VBIEscCol129921_1599.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96235 Genomic DNA. Translation: AAC16394.2 .
    U00096 Genomic DNA. Translation: AAC74603.2 .
    AP009048 Genomic DNA. Translation: BAA15220.1 .
    PIRi E64907.
    RefSeqi NP_416047.4. NC_000913.3.
    YP_489793.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JGS X-ray 2.30 A 7-144 [» ]
    3VB2 X-ray 2.60 A/B 1-144 [» ]
    3VOD X-ray 2.60 A/B 1-144 [» ]
    3VOE X-ray 2.60 A/B 1-144 [» ]
    4JBA X-ray 2.50 A/B 1-144 [» ]
    ProteinModelPortali P27245.
    SMRi P27245. Positions 7-144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10164N.
    IntActi P27245. 1 interaction.
    STRINGi 511145.b1530.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74603 ; AAC74603 ; b1530 .
    BAA15220 ; BAA15220 ; BAA15220 .
    GeneIDi 12930666.
    945825.
    KEGGi ecj:Y75_p1505.
    eco:b1530.
    PATRICi 32118358. VBIEscCol129921_1599.

    Organism-specific databases

    EchoBASEi EB1405.
    EcoGenei EG11435. marR.

    Phylogenomic databases

    eggNOGi COG1846.
    HOGENOMi HOG000221449.
    KOi K03712.
    OrthoDBi EOG6PGKBH.
    PhylomeDBi P27245.

    Enzyme and pathway databases

    BioCyci EcoCyc:PD00364.
    ECOL316407:JW5248-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P27245.
    PROi P27245.

    Gene expression databases

    Genevestigatori P27245.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR000835. HTH_MarR-typ.
    IPR023187. Tscrpt_reg_MarR-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF01047. MarR. 1 hit.
    [Graphical view ]
    PRINTSi PR00598. HTHMARR.
    SMARTi SM00347. HTH_MARR. 1 hit.
    [Graphical view ]
    PROSITEi PS01117. HTH_MARR_1. 1 hit.
    PS50995. HTH_MARR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli."
      Cohen S.P., Haechler H., Levy S.B.
      J. Bacteriol. 175:1484-1492(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Repressor mutations in the marRAB operon that activate oxidative stress genes and multiple antibiotic resistance in Escherichia coli."
      Ariza R.R., Cohen S.P., Bachhawat N., Levy S.B., Demple B.
      J. Bacteriol. 176:143-148(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Characterization of MarR, the repressor of the multiple antibiotic resistance (mar) operon in Escherichia coli."
      Seoane A.S., Levy S.B.
      J. Bacteriol. 177:3414-3419(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. "Mutational analysis of MarR, the negative regulator of marRAB expression in Escherichia coli, suggests the presence of two regions required for DNA binding."
      Alekshun M.N., Kim Y.S., Levy S.B.
      Mol. Microbiol. 35:1394-1404(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.

    Entry informationi

    Entry nameiMARR_ECOLI
    AccessioniPrimary (citable) accession number: P27245
    Secondary accession number(s): P76882, P77582
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3