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P27245

- MARR_ECOLI

UniProt

P27245 - MARR_ECOLI

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Protein

Multiple antibiotic resistance protein MarR

Gene

marR

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Repressor of the marRAB operon which is involved in the activation of both antibiotic resistance and oxidative stress genes. Binds to the marO operator/promoter site.

GO - Molecular functioni

  1. DNA binding Source: EcoCyc
  2. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. cellular response to antibiotic Source: EcoCyc
  2. negative regulation of transcription, DNA-templated Source: EcoCyc
  3. response to heat Source: EcoCyc
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Antibiotic resistance, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00364.
ECOL316407:JW5248-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Multiple antibiotic resistance protein MarR
Gene namesi
Name:marR
Synonyms:cfxB, inaR, soxQ
Ordered Locus Names:b1530, JW5248
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11435. marR.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi45 – 451V → E: Increased transcription of the region II transcript. 1 Publication
Mutagenesisi77 – 771R → L: Increased transcription of the region II transcript. 1 Publication
Mutagenesisi123 – 14422Missing: Increased transcription of the region II transcript. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 144144Multiple antibiotic resistance protein MarRPRO_0000054362Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP27245.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
gyrAP0AES42EBI-6409744,EBI-547129

Protein-protein interaction databases

DIPiDIP-10164N.
IntActiP27245. 1 interaction.
STRINGi511145.b1530.

Structurei

Secondary structure

1
144
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 3320
Turni34 – 374
Helixi40 – 5213
Beta strandi53 – 553
Helixi57 – 648
Helixi68 – 8013
Beta strandi83 – 886
Turni90 – 923
Beta strandi96 – 1005
Helixi102 – 12423
Turni125 – 1306
Helixi132 – 1409

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JGSX-ray2.30A7-144[»]
3VB2X-ray2.60A/B1-144[»]
3VODX-ray2.60A/B1-144[»]
3VOEX-ray2.60A/B1-144[»]
4JBAX-ray2.50A/B1-144[»]
ProteinModelPortaliP27245.
SMRiP27245. Positions 7-144.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27245.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 144134HTH marR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH marR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1846.
HOGENOMiHOG000221449.
InParanoidiP27245.
KOiK03712.
OrthoDBiEOG6PGKBH.
PhylomeDBiP27245.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000835. HTH_MarR-typ.
IPR023187. Tscrpt_reg_MarR-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01047. MarR. 1 hit.
[Graphical view]
PRINTSiPR00598. HTHMARR.
SMARTiSM00347. HTH_MARR. 1 hit.
[Graphical view]
PROSITEiPS01117. HTH_MARR_1. 1 hit.
PS50995. HTH_MARR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27245-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSTSDLFNE IIPLGRLIHM VNQKKDRLLN EYLSPLDITA AQFKVLCSIR
60 70 80 90 100
CAACITPVEL KKVLSVDLGA LTRMLDRLVC KGWVERLPNP NDKRGVLVKL
110 120 130 140
TTGGAAICEQ CHQLVGQDLH QELTKNLTAD EVATLEYLLK KVLP
Length:144
Mass (Da):16,065
Last modified:December 1, 2000 - v2
Checksum:iBE7DF5549E24D1D3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96235 Genomic DNA. Translation: AAC16394.2.
U00096 Genomic DNA. Translation: AAC74603.2.
AP009048 Genomic DNA. Translation: BAA15220.1.
PIRiE64907.
RefSeqiNP_416047.4. NC_000913.3.
YP_489793.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74603; AAC74603; b1530.
BAA15220; BAA15220; BAA15220.
GeneIDi12930666.
945825.
KEGGiecj:Y75_p1505.
eco:b1530.
PATRICi32118358. VBIEscCol129921_1599.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96235 Genomic DNA. Translation: AAC16394.2 .
U00096 Genomic DNA. Translation: AAC74603.2 .
AP009048 Genomic DNA. Translation: BAA15220.1 .
PIRi E64907.
RefSeqi NP_416047.4. NC_000913.3.
YP_489793.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JGS X-ray 2.30 A 7-144 [» ]
3VB2 X-ray 2.60 A/B 1-144 [» ]
3VOD X-ray 2.60 A/B 1-144 [» ]
3VOE X-ray 2.60 A/B 1-144 [» ]
4JBA X-ray 2.50 A/B 1-144 [» ]
ProteinModelPortali P27245.
SMRi P27245. Positions 7-144.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10164N.
IntActi P27245. 1 interaction.
STRINGi 511145.b1530.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74603 ; AAC74603 ; b1530 .
BAA15220 ; BAA15220 ; BAA15220 .
GeneIDi 12930666.
945825.
KEGGi ecj:Y75_p1505.
eco:b1530.
PATRICi 32118358. VBIEscCol129921_1599.

Organism-specific databases

EchoBASEi EB1405.
EcoGenei EG11435. marR.

Phylogenomic databases

eggNOGi COG1846.
HOGENOMi HOG000221449.
InParanoidi P27245.
KOi K03712.
OrthoDBi EOG6PGKBH.
PhylomeDBi P27245.

Enzyme and pathway databases

BioCyci EcoCyc:PD00364.
ECOL316407:JW5248-MONOMER.

Miscellaneous databases

EvolutionaryTracei P27245.
PROi P27245.

Gene expression databases

Genevestigatori P27245.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR000835. HTH_MarR-typ.
IPR023187. Tscrpt_reg_MarR-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF01047. MarR. 1 hit.
[Graphical view ]
PRINTSi PR00598. HTHMARR.
SMARTi SM00347. HTH_MARR. 1 hit.
[Graphical view ]
PROSITEi PS01117. HTH_MARR_1. 1 hit.
PS50995. HTH_MARR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli."
    Cohen S.P., Haechler H., Levy S.B.
    J. Bacteriol. 175:1484-1492(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Repressor mutations in the marRAB operon that activate oxidative stress genes and multiple antibiotic resistance in Escherichia coli."
    Ariza R.R., Cohen S.P., Bachhawat N., Levy S.B., Demple B.
    J. Bacteriol. 176:143-148(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Characterization of MarR, the repressor of the multiple antibiotic resistance (mar) operon in Escherichia coli."
    Seoane A.S., Levy S.B.
    J. Bacteriol. 177:3414-3419(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "Mutational analysis of MarR, the negative regulator of marRAB expression in Escherichia coli, suggests the presence of two regions required for DNA binding."
    Alekshun M.N., Kim Y.S., Levy S.B.
    Mol. Microbiol. 35:1394-1404(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiMARR_ECOLI
AccessioniPrimary (citable) accession number: P27245
Secondary accession number(s): P76882, P77582
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3