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Protein

O-antigen ligase

Gene

rfaL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Adds the O-antigen on the glucose group of LPS.

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

  • lipopolysaccharide core region biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11424-MONOMER.
ECOL316407:JW3597-MONOMER.
MetaCyc:EG11424-MONOMER.
UniPathwayiUPA00958.

Protein family/group databases

TCDBi9.B.67.5.1. the putative inorganic carbon (hco3(-)) transporter/o-antigen polymerase (ict/oap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
O-antigen ligase
Gene namesi
Name:rfaL
Synonyms:waaL
Ordered Locus Names:b3622, JW3597
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11424. rfaL.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1717CytoplasmicSequence analysisAdd
BLAST
Transmembranei18 – 3821HelicalSequence analysisAdd
BLAST
Topological domaini39 – 391PeriplasmicSequence analysis
Transmembranei40 – 6021HelicalSequence analysisAdd
BLAST
Topological domaini61 – 7111CytoplasmicSequence analysisAdd
BLAST
Transmembranei72 – 9221HelicalSequence analysisAdd
BLAST
Topological domaini93 – 10513PeriplasmicSequence analysisAdd
BLAST
Transmembranei106 – 12621HelicalSequence analysisAdd
BLAST
Topological domaini127 – 1359CytoplasmicSequence analysis
Transmembranei136 – 15621HelicalSequence analysisAdd
BLAST
Topological domaini157 – 16812PeriplasmicSequence analysisAdd
BLAST
Transmembranei169 – 18921HelicalSequence analysisAdd
BLAST
Topological domaini190 – 1934CytoplasmicSequence analysis
Transmembranei194 – 21421HelicalSequence analysisAdd
BLAST
Topological domaini215 – 2151PeriplasmicSequence analysis
Transmembranei216 – 23621HelicalSequence analysisAdd
BLAST
Topological domaini237 – 2415CytoplasmicSequence analysis
Transmembranei242 – 26221HelicalSequence analysisAdd
BLAST
Topological domaini263 – 34684PeriplasmicSequence analysisAdd
BLAST
Transmembranei347 – 36721HelicalSequence analysisAdd
BLAST
Topological domaini368 – 3692CytoplasmicSequence analysis
Transmembranei370 – 39021HelicalSequence analysisAdd
BLAST
Transmembranei391 – 41121HelicalSequence analysisAdd
BLAST
Topological domaini412 – 4198CytoplasmicSequence analysis

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419O-antigen ligasePRO_0000097284Add
BLAST

Proteomic databases

PaxDbiP27243.
PRIDEiP27243.

Interactioni

Protein-protein interaction databases

BioGridi4263298. 154 interactions.
DIPiDIP-10673N.
IntActiP27243. 1 interaction.
MINTiMINT-1286025.
STRINGi511145.b3622.

Structurei

3D structure databases

ProteinModelPortaliP27243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41068PG. Bacteria.
ENOG410XW26. LUCA.
HOGENOMiHOG000054845.
KOiK02847.
OMAiMLNELRY.
OrthoDBiEOG6C5RM0.

Family and domain databases

InterProiIPR007016. O-antigen_ligase-related.
[Graphical view]
PfamiPF04932. Wzy_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTSFKLHSL KPYTLKSSMI LEIITYILCF FSMIIAFVDN TFSIKIYNIT
60 70 80 90 100
AIVCLLSLIL RGRQENYNIK NLILPLSIFL IGLLDLIWYS AFKVDNSPFR
110 120 130 140 150
ATYHSYLNTA KIFIFGSFIV FLTLTSQLKS KKESVLYTLY SLSFLIAGYA
160 170 180 190 200
MYINSIHEND RISFGVGTAT GAAYSTMLIG IVSGVAILYT KKNHPFLFLL
210 220 230 240 250
NSCAVLYVLA LTQTRATLLL FPIICVAALI AYYNKSPKKF TSSIVLLIAI
260 270 280 290 300
LASIVIIFNK PIQNRYNEAL NDLNSYTNAN SVTSLGARLA MYEIGLNIFI
310 320 330 340 350
KSPFSFRSAE SRAESMNLLV AEHNRLRGAL EFSNVHLHNE IIEAGSLKGL
360 370 380 390 400
MGIFSTLFLY FSLFYIAYKK RALGLLILTL GIVGIGLSDV IIWARSIPII
410
IISAIVLLLV INNRNNTIN
Length:419
Mass (Da):46,878
Last modified:August 29, 2003 - v2
Checksum:iE15382BD2CC70533
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti205 – 22420VLYVL…LFPII → GTLCSGANTNQSNPTPVPYN in AAA24524 (PubMed:1624462).CuratedAdd
BLAST
Sequence conflicti307 – 3071R → T in AAA24524 (PubMed:1624462).Curated
Sequence conflicti344 – 3441A → R in AAA24524 (PubMed:1624462).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95398 Genomic DNA. Translation: AAA24524.1.
U00039 Genomic DNA. Translation: AAB18599.1.
U00096 Genomic DNA. Translation: AAC76646.1.
AP009048 Genomic DNA. Translation: BAE77670.1.
PIRiS47843.
RefSeqiNP_418079.1. NC_000913.3.
WP_001395405.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76646; AAC76646; b3622.
BAE77670; BAE77670; BAE77670.
GeneIDi948148.
KEGGiecj:JW3597.
eco:b3622.
PATRICi32122731. VBIEscCol129921_3742.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95398 Genomic DNA. Translation: AAA24524.1.
U00039 Genomic DNA. Translation: AAB18599.1.
U00096 Genomic DNA. Translation: AAC76646.1.
AP009048 Genomic DNA. Translation: BAE77670.1.
PIRiS47843.
RefSeqiNP_418079.1. NC_000913.3.
WP_001395405.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP27243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263298. 154 interactions.
DIPiDIP-10673N.
IntActiP27243. 1 interaction.
MINTiMINT-1286025.
STRINGi511145.b3622.

Protein family/group databases

TCDBi9.B.67.5.1. the putative inorganic carbon (hco3(-)) transporter/o-antigen polymerase (ict/oap) family.

Proteomic databases

PaxDbiP27243.
PRIDEiP27243.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76646; AAC76646; b3622.
BAE77670; BAE77670; BAE77670.
GeneIDi948148.
KEGGiecj:JW3597.
eco:b3622.
PATRICi32122731. VBIEscCol129921_3742.

Organism-specific databases

EchoBASEiEB1394.
EcoGeneiEG11424. rfaL.

Phylogenomic databases

eggNOGiENOG41068PG. Bacteria.
ENOG410XW26. LUCA.
HOGENOMiHOG000054845.
KOiK02847.
OMAiMLNELRY.
OrthoDBiEOG6C5RM0.

Enzyme and pathway databases

UniPathwayiUPA00958.
BioCyciEcoCyc:EG11424-MONOMER.
ECOL316407:JW3597-MONOMER.
MetaCyc:EG11424-MONOMER.

Miscellaneous databases

PROiP27243.

Family and domain databases

InterProiIPR007016. O-antigen_ligase-related.
[Graphical view]
PfamiPF04932. Wzy_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium."
    Klena J.D., Pradel E., Schnaitman C.A.
    J. Bacteriol. 174:4746-4752(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiRFAL_ECOLI
AccessioniPrimary (citable) accession number: P27243
Secondary accession number(s): Q2M7T6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 29, 2003
Last modified: January 20, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.