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Protein

O-antigen ligase

Gene

rfaL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adds the O-antigen on the glucose group of LPS.

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

  • lipopolysaccharide core region biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionLigase
Biological processLipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11424-MONOMER
MetaCyc:EG11424-MONOMER
UniPathwayiUPA00958

Protein family/group databases

TCDBi9.B.67.5.1 the o-antigen polymerase (oap) family

Names & Taxonomyi

Protein namesi
Recommended name:
O-antigen ligase
Gene namesi
Name:rfaL
Synonyms:waaL
Ordered Locus Names:b3622, JW3597
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11424 rfaL

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 17CytoplasmicSequence analysisAdd BLAST17
Transmembranei18 – 38HelicalSequence analysisAdd BLAST21
Topological domaini39PeriplasmicSequence analysis1
Transmembranei40 – 60HelicalSequence analysisAdd BLAST21
Topological domaini61 – 71CytoplasmicSequence analysisAdd BLAST11
Transmembranei72 – 92HelicalSequence analysisAdd BLAST21
Topological domaini93 – 105PeriplasmicSequence analysisAdd BLAST13
Transmembranei106 – 126HelicalSequence analysisAdd BLAST21
Topological domaini127 – 135CytoplasmicSequence analysis9
Transmembranei136 – 156HelicalSequence analysisAdd BLAST21
Topological domaini157 – 168PeriplasmicSequence analysisAdd BLAST12
Transmembranei169 – 189HelicalSequence analysisAdd BLAST21
Topological domaini190 – 193CytoplasmicSequence analysis4
Transmembranei194 – 214HelicalSequence analysisAdd BLAST21
Topological domaini215PeriplasmicSequence analysis1
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Topological domaini237 – 241CytoplasmicSequence analysis5
Transmembranei242 – 262HelicalSequence analysisAdd BLAST21
Topological domaini263 – 346PeriplasmicSequence analysisAdd BLAST84
Transmembranei347 – 367HelicalSequence analysisAdd BLAST21
Topological domaini368 – 369CytoplasmicSequence analysis2
Transmembranei370 – 390HelicalSequence analysisAdd BLAST21
Transmembranei391 – 411HelicalSequence analysisAdd BLAST21
Topological domaini412 – 419CytoplasmicSequence analysis8

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000972841 – 419O-antigen ligaseAdd BLAST419

Proteomic databases

PaxDbiP27243
PRIDEiP27243

Interactioni

Protein-protein interaction databases

BioGridi4263298, 155 interactors
DIPiDIP-10673N
IntActiP27243, 1 interactor
STRINGi316385.ECDH10B_3804

Structurei

3D structure databases

ProteinModelPortaliP27243
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41068PG Bacteria
ENOG410XW26 LUCA
HOGENOMiHOG000054845
KOiK02847
OMAiMLNELRY

Family and domain databases

InterProiView protein in InterPro
IPR007016 O-antigen_ligase-related
PfamiView protein in Pfam
PF04932 Wzy_C, 1 hit

Sequencei

Sequence statusi: Complete.

P27243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTSFKLHSL KPYTLKSSMI LEIITYILCF FSMIIAFVDN TFSIKIYNIT
60 70 80 90 100
AIVCLLSLIL RGRQENYNIK NLILPLSIFL IGLLDLIWYS AFKVDNSPFR
110 120 130 140 150
ATYHSYLNTA KIFIFGSFIV FLTLTSQLKS KKESVLYTLY SLSFLIAGYA
160 170 180 190 200
MYINSIHEND RISFGVGTAT GAAYSTMLIG IVSGVAILYT KKNHPFLFLL
210 220 230 240 250
NSCAVLYVLA LTQTRATLLL FPIICVAALI AYYNKSPKKF TSSIVLLIAI
260 270 280 290 300
LASIVIIFNK PIQNRYNEAL NDLNSYTNAN SVTSLGARLA MYEIGLNIFI
310 320 330 340 350
KSPFSFRSAE SRAESMNLLV AEHNRLRGAL EFSNVHLHNE IIEAGSLKGL
360 370 380 390 400
MGIFSTLFLY FSLFYIAYKK RALGLLILTL GIVGIGLSDV IIWARSIPII
410
IISAIVLLLV INNRNNTIN
Length:419
Mass (Da):46,878
Last modified:August 29, 2003 - v2
Checksum:iE15382BD2CC70533
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti205 – 224VLYVL…LFPII → GTLCSGANTNQSNPTPVPYN in AAA24524 (PubMed:1624462).CuratedAdd BLAST20
Sequence conflicti307R → T in AAA24524 (PubMed:1624462).Curated1
Sequence conflicti344A → R in AAA24524 (PubMed:1624462).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95398 Genomic DNA Translation: AAA24524.1
U00039 Genomic DNA Translation: AAB18599.1
U00096 Genomic DNA Translation: AAC76646.1
AP009048 Genomic DNA Translation: BAE77670.1
PIRiS47843
RefSeqiNP_418079.1, NC_000913.3
WP_001395405.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76646; AAC76646; b3622
BAE77670; BAE77670; BAE77670
GeneIDi948148
KEGGiecj:JW3597
eco:b3622
PATRICifig|1411691.4.peg.3084

Similar proteinsi

Entry informationi

Entry nameiRFAL_ECOLI
AccessioniPrimary (citable) accession number: P27243
Secondary accession number(s): Q2M7T6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 29, 2003
Last modified: March 28, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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