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Protein

Lipopolysaccharide 1,2-N-acetylglucosaminetransferase

Gene

waaU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein predictedi

Functioni

Adds the terminal N-acetyl-D-glucosamine group on the glucose(II) group of LPS.

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + lipopolysaccharide = UDP + N-acetyl-alpha-D-glucosaminyllipopolysaccharide.

Pathwayi

GO - Molecular functioni

  1. lipopolysaccharide N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  2. transferase activity, transferring glycosyl groups Source: EcoCyc

GO - Biological processi

  1. lipopolysaccharide core region biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11423-MONOMER.
ECOL316407:JW3598-MONOMER.
MetaCyc:EG11423-MONOMER.
UniPathwayiUPA00958.

Protein family/group databases

CAZyiGT9. Glycosyltransferase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide 1,2-N-acetylglucosaminetransferase (EC:2.4.1.56)
Gene namesi
Name:waaU
Synonyms:rfaK, waaK
Ordered Locus Names:b3623, JW3598
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11423. waaU.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Lipopolysaccharide 1,2-N-acetylglucosaminetransferasePRO_0000097283Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP27242.

Interactioni

Protein-protein interaction databases

DIPiDIP-10672N.
IntActiP27242. 7 interactions.
MINTiMINT-1231283.
STRINGi511145.b3623.

Structurei

3D structure databases

ProteinModelPortaliP27242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG0859.
HOGENOMiHOG000054846.
InParanoidiP27242.
KOiK03277.
OMAiHIASGFN.
OrthoDBiEOG6B8XFH.
PhylomeDBiP27242.

Family and domain databases

InterProiIPR002201. Glyco_trans_9.
[Graphical view]
PfamiPF01075. Glyco_transf_9. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27242-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLGTFHKKK RFYINKIKIN FLSFLFRNKI NNQITDPAQV KSCLIIHDNN
60 70 80 90 100
KLGDLIVLSS IYRELYSKGV KITLLTNRKG GEFLSNNKNI FEFCIKESTG
110 120 130 140 150
FLEMLTLCKH LRDLQFDIVL DPFETMPSFK HSLILSSLKD SYILGFDHWY
160 170 180 190 200
KRYYSFYHPH DECLKEHMST RAIEILKHIY GEGKFSTNYD LHLPVDVEDK
210 220 230 240 250
IKEFIGDTRI VIINPLGAKK ICRLTFEQIK VIYQEVKTHF ENYRIIFTGL
260 270 280 290 300
PQDLLTIPIL EIETLPFDEF IYTVALTKYS DFVISVDTAL VHIAAAYHKP
310 320 330 340 350
TLAFYPNSRT PEYPSHLIWS PNHHKSIQIV SPTYTVKDID TETLTNSVKR

LSCIDKK
Length:357
Mass (Da):41,729
Last modified:October 1, 1994 - v2
Checksum:i951523B8192616F5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti293 – 2931I → M in AAA24523. (PubMed:1624462)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95398 Genomic DNA. Translation: AAA24523.1.
U00039 Genomic DNA. Translation: AAB18600.1.
U00096 Genomic DNA. Translation: AAC76647.1.
AP009048 Genomic DNA. Translation: BAE77669.1.
PIRiA65163.
RefSeqiNP_418080.1. NC_000913.3.
YP_491810.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76647; AAC76647; b3623.
BAE77669; BAE77669; BAE77669.
GeneIDi12934311.
948147.
KEGGiecj:Y75_p3551.
eco:b3623.
PATRICi32122733. VBIEscCol129921_3743.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95398 Genomic DNA. Translation: AAA24523.1.
U00039 Genomic DNA. Translation: AAB18600.1.
U00096 Genomic DNA. Translation: AAC76647.1.
AP009048 Genomic DNA. Translation: BAE77669.1.
PIRiA65163.
RefSeqiNP_418080.1. NC_000913.3.
YP_491810.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP27242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10672N.
IntActiP27242. 7 interactions.
MINTiMINT-1231283.
STRINGi511145.b3623.

Protein family/group databases

CAZyiGT9. Glycosyltransferase Family 9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76647; AAC76647; b3623.
BAE77669; BAE77669; BAE77669.
GeneIDi12934311.
948147.
KEGGiecj:Y75_p3551.
eco:b3623.
PATRICi32122733. VBIEscCol129921_3743.

Organism-specific databases

EchoBASEiEB1393.
EcoGeneiEG11423. waaU.

Phylogenomic databases

eggNOGiCOG0859.
HOGENOMiHOG000054846.
InParanoidiP27242.
KOiK03277.
OMAiHIASGFN.
OrthoDBiEOG6B8XFH.
PhylomeDBiP27242.

Enzyme and pathway databases

UniPathwayiUPA00958.
BioCyciEcoCyc:EG11423-MONOMER.
ECOL316407:JW3598-MONOMER.
MetaCyc:EG11423-MONOMER.

Miscellaneous databases

PROiP27242.

Gene expression databases

GenevestigatoriP27242.

Family and domain databases

InterProiIPR002201. Glyco_trans_9.
[Graphical view]
PfamiPF01075. Glyco_transf_9. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium."
    Klena J.D., Pradel E., Schnaitman C.A.
    J. Bacteriol. 174:4746-4752(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiWAAU_ECOLI
AccessioniPrimary (citable) accession number: P27242
Secondary accession number(s): Q2M7T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1994
Last modified: January 7, 2015
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.