P27242 (WAAU_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipopolysaccharide 1,2-N-acetylglucosaminetransferase
EC=2.4.1.56

Gene names

Name:	waaU
Synonyms:	rfaK, waaK
Ordered Locus Names:	b3623, JW3598

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Adds the terminal N-acetyl-D-glucosamine group on the glucose(II) group of LPS.
Catalytic activity	UDP-N-acetyl-alpha-D-glucosamine + lipopolysaccharide = UDP + N-acetyl-alpha-D-glucosaminyllipopolysaccharide.
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Subcellular location	Membrane; Peripheral membrane protein.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Membrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipopolysaccharide core region biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lipopolysaccharide N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Lipopolysaccharide 1,2-N-acetylglucosaminetransferase		PRO_0000097283
Experimental info
	Sequence conflict	293	1	I → M in AAA24523. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P27242 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 951523B8192616F5	FASTA	357	41,729
	10 20 30 40 50 60 MRLGTFHKKK RFYINKIKIN FLSFLFRNKI NNQITDPAQV KSCLIIHDNN KLGDLIVLSS 70 80 90 100 110 120 IYRELYSKGV KITLLTNRKG GEFLSNNKNI FEFCIKESTG FLEMLTLCKH LRDLQFDIVL 130 140 150 160 170 180 DPFETMPSFK HSLILSSLKD SYILGFDHWY KRYYSFYHPH DECLKEHMST RAIEILKHIY 190 200 210 220 230 240 GEGKFSTNYD LHLPVDVEDK IKEFIGDTRI VIINPLGAKK ICRLTFEQIK VIYQEVKTHF 250 260 270 280 290 300 ENYRIIFTGL PQDLLTIPIL EIETLPFDEF IYTVALTKYS DFVISVDTAL VHIAAAYHKP 310 320 330 340 350 TLAFYPNSRT PEYPSHLIWS PNHHKSIQIV SPTYTVKDID TETLTNSVKR LSCIDKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium." Klena J.D., Pradel E., Schnaitman C.A. J. Bacteriol. 174:4746-4752(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95398 Genomic DNA. Translation: AAA24523.1. U00039 Genomic DNA. Translation: AAB18600.1. U00096 Genomic DNA. Translation: AAC76647.1. AP009048 Genomic DNA. Translation: BAE77669.1.
PIR	A65163.
RefSeq	NP_418080.1. NC_000913.2. YP_491810.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P27242.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10672N.
IntAct	P27242. 7 interactions.
MINT	MINT-1231283.
STRING	511145.b3623.
Protein family/group databases
CAZy	GT9. Glycosyltransferase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76647; AAC76647; b3623. BAE77669; BAE77669; BAE77669.
GeneID	12934311. 948147.
KEGG	ecj:Y75_p3551. eco:b3623.
PATRIC	32122733. VBIEscCol129921_3743.
Organism-specific databases
EchoBASE	EB1393.
EcoGene	EG11423. waaU.
Phylogenomic databases
eggNOG	COG0859.
HOGENOM	HOG000054846.
KO	K03277.
OMA	HIASGFN.
ProtClustDB	CLSK2301716.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11423-MONOMER. ECOL316407:JW3598-MONOMER. MetaCyc:EG11423-MONOMER.
UniPathway	UPA00958.
Gene expression databases
Genevestigator	P27242.
Family and domain databases
InterPro	IPR002201. Glyco_trans_9. [Graphical view]
Pfam	PF01075. Glyco_transf_9. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WAAU_ECOLI

Accession

Primary (citable) accession number: P27242
Secondary accession number(s): Q2M7T7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program