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P27242 (WAAU_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipopolysaccharide 1,2-N-acetylglucosaminetransferase

EC=2.4.1.56
Gene names
Name:waaU
Synonyms:rfaK, waaK
Ordered Locus Names:b3623, JW3598
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Adds the terminal N-acetyl-D-glucosamine group on the glucose(II) group of LPS.

Catalytic activity

UDP-N-acetyl-alpha-D-glucosamine + lipopolysaccharide = UDP + N-acetyl-alpha-D-glucosaminyllipopolysaccharide.

Pathway

Bacterial outer membrane biogenesis; LPS core biosynthesis.

Subcellular location

Membrane; Peripheral membrane protein.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Lipopolysaccharide 1,2-N-acetylglucosaminetransferase
PRO_0000097283

Experimental info

Sequence conflict2931I → M in AAA24523. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P27242 [UniParc].

Last modified October 1, 1994. Version 2.
Checksum: 951523B8192616F5

FASTA35741,729
        10         20         30         40         50         60 
MRLGTFHKKK RFYINKIKIN FLSFLFRNKI NNQITDPAQV KSCLIIHDNN KLGDLIVLSS 

        70         80         90        100        110        120 
IYRELYSKGV KITLLTNRKG GEFLSNNKNI FEFCIKESTG FLEMLTLCKH LRDLQFDIVL 

       130        140        150        160        170        180 
DPFETMPSFK HSLILSSLKD SYILGFDHWY KRYYSFYHPH DECLKEHMST RAIEILKHIY 

       190        200        210        220        230        240 
GEGKFSTNYD LHLPVDVEDK IKEFIGDTRI VIINPLGAKK ICRLTFEQIK VIYQEVKTHF 

       250        260        270        280        290        300 
ENYRIIFTGL PQDLLTIPIL EIETLPFDEF IYTVALTKYS DFVISVDTAL VHIAAAYHKP 

       310        320        330        340        350 
TLAFYPNSRT PEYPSHLIWS PNHHKSIQIV SPTYTVKDID TETLTNSVKR LSCIDKK 

« Hide

References

« Hide 'large scale' references
[1]"Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium."
Klena J.D., Pradel E., Schnaitman C.A.
J. Bacteriol. 174:4746-4752(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M95398 Genomic DNA. Translation: AAA24523.1.
U00039 Genomic DNA. Translation: AAB18600.1.
U00096 Genomic DNA. Translation: AAC76647.1.
AP009048 Genomic DNA. Translation: BAE77669.1.
PIRA65163.
RefSeqNP_418080.1. NC_000913.3.
YP_491810.1. NC_007779.1.

3D structure databases

ProteinModelPortalP27242.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10672N.
IntActP27242. 7 interactions.
MINTMINT-1231283.
STRING511145.b3623.

Protein family/group databases

CAZyGT9. Glycosyltransferase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76647; AAC76647; b3623.
BAE77669; BAE77669; BAE77669.
GeneID12934311.
948147.
KEGGecj:Y75_p3551.
eco:b3623.
PATRIC32122733. VBIEscCol129921_3743.

Organism-specific databases

EchoBASEEB1393.
EcoGeneEG11423. waaU.

Phylogenomic databases

eggNOGCOG0859.
HOGENOMHOG000054846.
KOK03277.
OMAHIASGFN.
OrthoDBEOG6B8XFH.
PhylomeDBP27242.

Enzyme and pathway databases

BioCycEcoCyc:EG11423-MONOMER.
ECOL316407:JW3598-MONOMER.
MetaCyc:EG11423-MONOMER.
UniPathwayUPA00958.

Gene expression databases

GenevestigatorP27242.

Family and domain databases

InterProIPR002201. Glyco_trans_9.
[Graphical view]
PfamPF01075. Glyco_transf_9. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP27242.

Entry information

Entry nameWAAU_ECOLI
AccessionPrimary (citable) accession number: P27242
Secondary accession number(s): Q2M7T7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1994
Last modified: May 14, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene