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P27240

- RFAY_ECOLI

UniProt

P27240 - RFAY_ECOLI

Protein

Lipopolysaccharide core heptose(II) kinase RfaY

Gene

rfaY

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of heptose(II) of the outer membrane lipopolysaccharide core.By similarity

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. lipopolysaccharide core region biosynthetic process Source: UniProtKB-UniPathway
    2. phosphorylation Source: EcoCyc

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipopolysaccharide biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11425-MONOMER.
    ECOL316407:JW3600-MONOMER.
    MetaCyc:EG11425-MONOMER.
    UniPathwayiUPA00958.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipopolysaccharide core heptose(II) kinase RfaY (EC:2.7.1.-)
    Gene namesi
    Name:rfaY
    Synonyms:waaY
    Ordered Locus Names:b3625, JW3600
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11425. rfaY.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 232232Lipopolysaccharide core heptose(II) kinase RfaYPRO_0000097288Add
    BLAST

    Proteomic databases

    PRIDEiP27240.

    Expressioni

    Gene expression databases

    GenevestigatoriP27240.

    Interactioni

    Protein-protein interaction databases

    IntActiP27240. 10 interactions.
    STRINGi511145.b3625.

    Structurei

    3D structure databases

    ProteinModelPortaliP27240.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG18264.
    HOGENOMiHOG000126191.
    KOiK02850.
    OMAiMIIEKKV.
    OrthoDBiEOG6H7FFG.
    PhylomeDBiP27240.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR009330. LipoPS_heptP_kinase.
    [Graphical view]
    PfamiPF06176. WaaY. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P27240-1 [UniParc]FASTAAdd to Basket

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    MIQKSKIKDL VVFTDENNSK YLNVLNDFLS YNINIIKVFR SIDDTKVMLI    50
    DTDYGKLILK VFSPKVKRNE RFFKSLLKGD YYERLFEQTQ KVRNEGLNTL 100
    NDFYLLAERK TLRFVHTYIM IIEYIDGIEL CDMPDIDDAL KNKIQQSINA 150
    LHQHGMVSGD PHRGNFIIKN GEVRIIDLSG KRASAQRKAK DRIDLERHYG 200
    IKNEIRDLGY YLLVYRKKMR NFMRRLKGKP AR 232
    Length:232
    Mass (Da):27,461
    Last modified:October 1, 1994 - v2
    Checksum:i69E97B9C0842EFDA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti51 – 522DT → VS in AAA24521. (PubMed:1624462)Curated
    Sequence conflicti145 – 1462QQ → HE in AAA24521. (PubMed:1624462)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95398 Genomic DNA. Translation: AAA24521.1.
    U00039 Genomic DNA. Translation: AAB18602.1.
    U00096 Genomic DNA. Translation: AAC76649.1.
    AP009048 Genomic DNA. Translation: BAE77667.1.
    PIRiS47846.
    RefSeqiNP_418082.1. NC_000913.3.
    YP_491808.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76649; AAC76649; b3625.
    BAE77667; BAE77667; BAE77667.
    GeneIDi12931666.
    948145.
    KEGGiecj:Y75_p3549.
    eco:b3625.
    PATRICi32122737. VBIEscCol129921_3745.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95398 Genomic DNA. Translation: AAA24521.1 .
    U00039 Genomic DNA. Translation: AAB18602.1 .
    U00096 Genomic DNA. Translation: AAC76649.1 .
    AP009048 Genomic DNA. Translation: BAE77667.1 .
    PIRi S47846.
    RefSeqi NP_418082.1. NC_000913.3.
    YP_491808.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P27240.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P27240. 10 interactions.
    STRINGi 511145.b3625.

    Proteomic databases

    PRIDEi P27240.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76649 ; AAC76649 ; b3625 .
    BAE77667 ; BAE77667 ; BAE77667 .
    GeneIDi 12931666.
    948145.
    KEGGi ecj:Y75_p3549.
    eco:b3625.
    PATRICi 32122737. VBIEscCol129921_3745.

    Organism-specific databases

    EchoBASEi EB1395.
    EcoGenei EG11425. rfaY.

    Phylogenomic databases

    eggNOGi NOG18264.
    HOGENOMi HOG000126191.
    KOi K02850.
    OMAi MIIEKKV.
    OrthoDBi EOG6H7FFG.
    PhylomeDBi P27240.

    Enzyme and pathway databases

    UniPathwayi UPA00958 .
    BioCyci EcoCyc:EG11425-MONOMER.
    ECOL316407:JW3600-MONOMER.
    MetaCyc:EG11425-MONOMER.

    Miscellaneous databases

    PROi P27240.

    Gene expression databases

    Genevestigatori P27240.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR009330. LipoPS_heptP_kinase.
    [Graphical view ]
    Pfami PF06176. WaaY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium."
      Klena J.D., Pradel E., Schnaitman C.A.
      J. Bacteriol. 174:4746-4752(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
      Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
      Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiRFAY_ECOLI
    AccessioniPrimary (citable) accession number: P27240
    Secondary accession number(s): Q2M7T9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3