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Protein

Lipopolysaccharide core heptose(II) kinase RfaY

Gene

rfaY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of heptose(II) of the outer membrane lipopolysaccharide core.By similarity

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: EcoCyc

GO - Biological processi

  • lipopolysaccharide core region biosynthetic process Source: UniProtKB-UniPathway
  • phosphorylation Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processLipopolysaccharide biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11425-MONOMER
MetaCyc:EG11425-MONOMER
UniPathwayiUPA00958

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide core heptose(II) kinase RfaY (EC:2.7.1.-)
Gene namesi
Name:rfaY
Synonyms:waaY
Ordered Locus Names:b3625, JW3600
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11425 rfaY

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000972881 – 232Lipopolysaccharide core heptose(II) kinase RfaYAdd BLAST232

Proteomic databases

PaxDbiP27240
PRIDEiP27240

Interactioni

Protein-protein interaction databases

BioGridi4260660, 327 interactors
IntActiP27240, 10 interactors
STRINGi316385.ECDH10B_3807

Structurei

3D structure databases

ProteinModelPortaliP27240
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108PDR Bacteria
ENOG4111F7R LUCA
HOGENOMiHOG000126191
InParanoidiP27240
KOiK02850
OMAiMIIEKKV
PhylomeDBiP27240

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR009330 LipoPS_heptP_kinase
PfamiView protein in Pfam
PF06176 WaaY, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits

Sequencei

Sequence statusi: Complete.

P27240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQKSKIKDL VVFTDENNSK YLNVLNDFLS YNINIIKVFR SIDDTKVMLI
60 70 80 90 100
DTDYGKLILK VFSPKVKRNE RFFKSLLKGD YYERLFEQTQ KVRNEGLNTL
110 120 130 140 150
NDFYLLAERK TLRFVHTYIM IIEYIDGIEL CDMPDIDDAL KNKIQQSINA
160 170 180 190 200
LHQHGMVSGD PHRGNFIIKN GEVRIIDLSG KRASAQRKAK DRIDLERHYG
210 220 230
IKNEIRDLGY YLLVYRKKMR NFMRRLKGKP AR
Length:232
Mass (Da):27,461
Last modified:October 1, 1994 - v2
Checksum:i69E97B9C0842EFDA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51 – 52DT → VS in AAA24521 (PubMed:1624462).Curated2
Sequence conflicti145 – 146QQ → HE in AAA24521 (PubMed:1624462).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95398 Genomic DNA Translation: AAA24521.1
U00039 Genomic DNA Translation: AAB18602.1
U00096 Genomic DNA Translation: AAC76649.1
AP009048 Genomic DNA Translation: BAE77667.1
PIRiS47846
RefSeqiNP_418082.1, NC_000913.3
WP_000615254.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76649; AAC76649; b3625
BAE77667; BAE77667; BAE77667
GeneIDi948145
KEGGiecj:JW3600
eco:b3625
PATRICifig|1411691.4.peg.3081

Similar proteinsi

Entry informationi

Entry nameiRFAY_ECOLI
AccessioniPrimary (citable) accession number: P27240
Secondary accession number(s): Q2M7T9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1994
Last modified: April 25, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health