P27240 (RFAY_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipopolysaccharide core heptose(II) kinase RfaY
EC=2.7.1.-

Gene names

Name:	rfaY
Synonyms:	waaY
Ordered Locus Names:	b3625, JW3600

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of heptose(II) of the outer membrane lipopolysaccharide core By similarity.
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Sequence similarities	Belongs to the protein kinase superfamily. RfaY/WaaY family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipopolysaccharide core region biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway phosphorylation Inferred from mutant phenotype PubMed 9756860. Source: EcoCyc
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Lipopolysaccharide core heptose(II) kinase RfaY		PRO_0000097288
Experimental info
	Sequence conflict	51 – 52	2	DT → VS in AAA24521. Ref.1
	Sequence conflict	145 – 146	2	QQ → HE in AAA24521. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P27240 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 69E97B9C0842EFDA	FASTA	232	27,461
	10 20 30 40 50 60 MIQKSKIKDL VVFTDENNSK YLNVLNDFLS YNINIIKVFR SIDDTKVMLI DTDYGKLILK 70 80 90 100 110 120 VFSPKVKRNE RFFKSLLKGD YYERLFEQTQ KVRNEGLNTL NDFYLLAERK TLRFVHTYIM 130 140 150 160 170 180 IIEYIDGIEL CDMPDIDDAL KNKIQQSINA LHQHGMVSGD PHRGNFIIKN GEVRIIDLSG 190 200 210 220 230 KRASAQRKAK DRIDLERHYG IKNEIRDLGY YLLVYRKKMR NFMRRLKGKP AR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium." Klena J.D., Pradel E., Schnaitman C.A. J. Bacteriol. 174:4746-4752(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95398 Genomic DNA. Translation: AAA24521.1. U00039 Genomic DNA. Translation: AAB18602.1. U00096 Genomic DNA. Translation: AAC76649.1. AP009048 Genomic DNA. Translation: BAE77667.1.
PIR	S47846.
RefSeq	NP_418082.1. NC_000913.2. YP_491808.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P27240.
ModBase	Search...
Protein-protein interaction databases
IntAct	P27240. 10 interactions.
STRING	511145.b3625.
Proteomic databases
PRIDE	P27240.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76649; AAC76649; b3625. BAE77667; BAE77667; BAE77667.
GeneID	12931666. 948145.
KEGG	ecj:Y75_p3549. eco:b3625.
PATRIC	32122737. VBIEscCol129921_3745.
Organism-specific databases
EchoBASE	EB1395.
EcoGene	EG11425. rfaY.
Phylogenomic databases
eggNOG	NOG18264.
HOGENOM	HOG000126191.
KO	K02850.
OMA	MIIEKKV.
ProtClustDB	PRK10359.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11425-MONOMER. ECOL316407:JW3600-MONOMER. MetaCyc:EG11425-MONOMER.
UniPathway	UPA00958.
Gene expression databases
Genevestigator	P27240.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR009330. LipoPS_heptP_kinase. [Graphical view]
Pfam	PF06176. WaaY. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RFAY_ECOLI

Accession

Primary (citable) accession number: P27240
Secondary accession number(s): Q2M7T9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program