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P27240 (RFAY_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipopolysaccharide core heptose(II) kinase RfaY

EC=2.7.1.-
Gene names
Name:rfaY
Synonyms:waaY
Ordered Locus Names:b3625, JW3600
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of heptose(II) of the outer membrane lipopolysaccharide core By similarity.

Pathway

Bacterial outer membrane biogenesis; LPS core biosynthesis.

Sequence similarities

Belongs to the protein kinase superfamily. RfaY/WaaY family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 232232Lipopolysaccharide core heptose(II) kinase RfaY
PRO_0000097288

Experimental info

Sequence conflict51 – 522DT → VS in AAA24521. Ref.1
Sequence conflict145 – 1462QQ → HE in AAA24521. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P27240 [UniParc].

Last modified October 1, 1994. Version 2.
Checksum: 69E97B9C0842EFDA

FASTA23227,461
        10         20         30         40         50         60 
MIQKSKIKDL VVFTDENNSK YLNVLNDFLS YNINIIKVFR SIDDTKVMLI DTDYGKLILK 

        70         80         90        100        110        120 
VFSPKVKRNE RFFKSLLKGD YYERLFEQTQ KVRNEGLNTL NDFYLLAERK TLRFVHTYIM 

       130        140        150        160        170        180 
IIEYIDGIEL CDMPDIDDAL KNKIQQSINA LHQHGMVSGD PHRGNFIIKN GEVRIIDLSG 

       190        200        210        220        230 
KRASAQRKAK DRIDLERHYG IKNEIRDLGY YLLVYRKKMR NFMRRLKGKP AR 

« Hide

References

« Hide 'large scale' references
[1]"Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium."
Klena J.D., Pradel E., Schnaitman C.A.
J. Bacteriol. 174:4746-4752(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M95398 Genomic DNA. Translation: AAA24521.1.
U00039 Genomic DNA. Translation: AAB18602.1.
U00096 Genomic DNA. Translation: AAC76649.1.
AP009048 Genomic DNA. Translation: BAE77667.1.
PIRS47846.
RefSeqNP_418082.1. NC_000913.3.
YP_491808.1. NC_007779.1.

3D structure databases

ProteinModelPortalP27240.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP27240. 10 interactions.
STRING511145.b3625.

Proteomic databases

PRIDEP27240.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76649; AAC76649; b3625.
BAE77667; BAE77667; BAE77667.
GeneID12931666.
948145.
KEGGecj:Y75_p3549.
eco:b3625.
PATRIC32122737. VBIEscCol129921_3745.

Organism-specific databases

EchoBASEEB1395.
EcoGeneEG11425. rfaY.

Phylogenomic databases

eggNOGNOG18264.
HOGENOMHOG000126191.
KOK02850.
OMAMIIEKKV.
OrthoDBEOG6H7FFG.
PhylomeDBP27240.
ProtClustDBPRK10359.

Enzyme and pathway databases

BioCycEcoCyc:EG11425-MONOMER.
ECOL316407:JW3600-MONOMER.
MetaCyc:EG11425-MONOMER.
UniPathwayUPA00958.

Gene expression databases

GenevestigatorP27240.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR009330. LipoPS_heptP_kinase.
[Graphical view]
PfamPF06176. WaaY. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 2 hits.
ProtoNetSearch...

Other

PROP27240.

Entry information

Entry nameRFAY_ECOLI
AccessionPrimary (citable) accession number: P27240
Secondary accession number(s): Q2M7T9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene