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Protein

Lipopolysaccharide core heptose(II) kinase RfaY

Gene

rfaY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of heptose(II) of the outer membrane lipopolysaccharide core.By similarity

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. lipopolysaccharide core region biosynthetic process Source: UniProtKB-UniPathway
  2. phosphorylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11425-MONOMER.
ECOL316407:JW3600-MONOMER.
MetaCyc:EG11425-MONOMER.
UniPathwayiUPA00958.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide core heptose(II) kinase RfaY (EC:2.7.1.-)
Gene namesi
Name:rfaY
Synonyms:waaY
Ordered Locus Names:b3625, JW3600
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11425. rfaY.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Lipopolysaccharide core heptose(II) kinase RfaYPRO_0000097288Add
BLAST

Proteomic databases

PRIDEiP27240.

Expressioni

Gene expression databases

GenevestigatoriP27240.

Interactioni

Protein-protein interaction databases

IntActiP27240. 10 interactions.
STRINGi511145.b3625.

Structurei

3D structure databases

ProteinModelPortaliP27240.
SMRiP27240. Positions 118-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG18264.
HOGENOMiHOG000126191.
InParanoidiP27240.
KOiK02850.
OMAiMIIEKKV.
OrthoDBiEOG6H7FFG.
PhylomeDBiP27240.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR009330. LipoPS_heptP_kinase.
[Graphical view]
PfamiPF06176. WaaY. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.

Sequencei

Sequence statusi: Complete.

P27240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQKSKIKDL VVFTDENNSK YLNVLNDFLS YNINIIKVFR SIDDTKVMLI
60 70 80 90 100
DTDYGKLILK VFSPKVKRNE RFFKSLLKGD YYERLFEQTQ KVRNEGLNTL
110 120 130 140 150
NDFYLLAERK TLRFVHTYIM IIEYIDGIEL CDMPDIDDAL KNKIQQSINA
160 170 180 190 200
LHQHGMVSGD PHRGNFIIKN GEVRIIDLSG KRASAQRKAK DRIDLERHYG
210 220 230
IKNEIRDLGY YLLVYRKKMR NFMRRLKGKP AR
Length:232
Mass (Da):27,461
Last modified:September 30, 1994 - v2
Checksum:i69E97B9C0842EFDA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 522DT → VS in AAA24521 (PubMed:1624462).Curated
Sequence conflicti145 – 1462QQ → HE in AAA24521 (PubMed:1624462).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95398 Genomic DNA. Translation: AAA24521.1.
U00039 Genomic DNA. Translation: AAB18602.1.
U00096 Genomic DNA. Translation: AAC76649.1.
AP009048 Genomic DNA. Translation: BAE77667.1.
PIRiS47846.
RefSeqiNP_418082.1. NC_000913.3.
YP_491808.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76649; AAC76649; b3625.
BAE77667; BAE77667; BAE77667.
GeneIDi12931666.
948145.
KEGGiecj:Y75_p3549.
eco:b3625.
PATRICi32122737. VBIEscCol129921_3745.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95398 Genomic DNA. Translation: AAA24521.1.
U00039 Genomic DNA. Translation: AAB18602.1.
U00096 Genomic DNA. Translation: AAC76649.1.
AP009048 Genomic DNA. Translation: BAE77667.1.
PIRiS47846.
RefSeqiNP_418082.1. NC_000913.3.
YP_491808.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP27240.
SMRiP27240. Positions 118-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP27240. 10 interactions.
STRINGi511145.b3625.

Proteomic databases

PRIDEiP27240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76649; AAC76649; b3625.
BAE77667; BAE77667; BAE77667.
GeneIDi12931666.
948145.
KEGGiecj:Y75_p3549.
eco:b3625.
PATRICi32122737. VBIEscCol129921_3745.

Organism-specific databases

EchoBASEiEB1395.
EcoGeneiEG11425. rfaY.

Phylogenomic databases

eggNOGiNOG18264.
HOGENOMiHOG000126191.
InParanoidiP27240.
KOiK02850.
OMAiMIIEKKV.
OrthoDBiEOG6H7FFG.
PhylomeDBiP27240.

Enzyme and pathway databases

UniPathwayiUPA00958.
BioCyciEcoCyc:EG11425-MONOMER.
ECOL316407:JW3600-MONOMER.
MetaCyc:EG11425-MONOMER.

Miscellaneous databases

PROiP27240.

Gene expression databases

GenevestigatoriP27240.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR009330. LipoPS_heptP_kinase.
[Graphical view]
PfamiPF06176. WaaY. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium."
    Klena J.D., Pradel E., Schnaitman C.A.
    J. Bacteriol. 174:4746-4752(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiRFAY_ECOLI
AccessioniPrimary (citable) accession number: P27240
Secondary accession number(s): Q2M7T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 31, 1992
Last sequence update: September 30, 1994
Last modified: March 31, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.