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P27219 (PTSBC_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
PTS system sucrose-specific EIIBC component
Alternative name(s):
EIIBC-Scr
Short name=EII-Scr

Including the following 2 domains:

  1. Sucrose-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
    Alternative name(s):
    PTS system sucrose-specific EIIB component
  2. Sucrose permease IIC component
    Alternative name(s):
    PTS system sucrose-specific EIIC component
Gene names
Name:scrA
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length456 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in sucrose transport.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell inner membrane; Multi-pass membrane protein Probable.

Domain

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

Sequence similarities

Contains 1 PTS EIIB type-1 domain.

Contains 1 PTS EIIC type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 456456PTS system sucrose-specific EIIBC component
PRO_0000186668

Regions

Transmembrane112 – 13221Helical; Potential
Transmembrane144 – 16421Helical; Potential
Transmembrane181 – 20121Helical; Potential
Transmembrane209 – 22921Helical; Potential
Transmembrane247 – 26721Helical; Potential
Transmembrane288 – 30821Helical; Potential
Transmembrane329 – 34921Helical; Potential
Transmembrane360 – 38021Helical; Potential
Transmembrane388 – 40821Helical; Potential
Transmembrane428 – 44821Helical; Potential
Domain1 – 8787PTS EIIB type-1
Domain107 – 456350PTS EIIC type-1

Sites

Active site261Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P27219 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 4AFDF5405CAEFC66

FASTA45648,022
        10         20         30         40         50         60 
MDFEQISRSL LPLLGGKENI ASAAHCATRL RLVLVDDALA DQQAIGKIDG VKGCFRNAGQ 

        70         80         90        100        110        120 
MQIIFGTGVV NKVYAAFIQA AGISESSKSE AADLAAKKLN PFQRIARLLS NIFVPIIPAI 

       130        140        150        160        170        180 
VASGLLMGLL GMVKTYGWVD PSNALYIMLD MCSSAAFIIL PILIGFTAAR EFGGNPYLGA 

       190        200        210        220        230        240 
TLGGILTHPA LTNAWGVAAG FHTMNFFGIE VAMIGYQGTV FPVLLAVWFM SMVEKRLRRV 

       250        260        270        280        290        300 
IPDALDLILT PFLTVIISGF IALLLIGPAG RALGDGISFI LSTLISHAGW LAGLLFGGLY 

       310        320        330        340        350        360 
SVIVITGIHH SFHAIEAGLL GNPSIGVNFL LPIWAMANVA QGGACFAVWF KTKDAKIKAI 

       370        380        390        400        410        420 
TLPSAFSAML GITEAAIFGI NLRFVKPFIA ALVGGAAGGA WVVSMHVYMT AVGLTAIPGM 

       430        440        450 
AIVQASSLLN YIIGMAIAFA VAFALSLTLK YKTDAE

« Hide

References

[1]"Molecular analysis of the scrA and scrB genes from Klebsiella pneumoniae and plasmid pUR400, which encode the sucrose transport protein Enzyme II Scr of the phosphotransferase system and a sucrose-6-phosphate invertase."
Titgemeyer F., Jahreis K., Ebner R., Lengeler J.W.
Mol. Gen. Genet. 250:197-206(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 1033-5P14 / KAY2026.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X57401 Genomic DNA. Translation: CAA40658.1.
PIRS62331.

3D structure databases

ProteinModelPortalP27219.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-12625.

Family and domain databases

Gene3D3.30.1360.60. 1 hit.
InterProIPR018113. PTrfase_EIIB/Cys_phosph_CS.
IPR004719. PTrfase_sys_maltose/Glc-sp_IIC.
IPR001996. PTS_EIIB_1.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR011535. PTS_Glc-like_IIB_component.
IPR010973. PTS_IIBC_sucr.
[Graphical view]
PfamPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMSSF55604. PTS_EIIB. 1 hit.
TIGRFAMsTIGR00826. EIIB_glc. 1 hit.
TIGR00852. pts-Glc. 1 hit.
TIGR01996. PTS-II-BC-sucr. 1 hit.
PROSITEPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTSBC_KLEPN
AccessionPrimary (citable) accession number: P27219
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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