Reviewed, UniProtKB/Swiss-Prot P27217 (SCRB_KLEPN)

Names and origin

Protein names

Recommended name:
    Sucrose-6-phosphate hydrolase
      Short name=Sucrase
    EC=3.2.1.26
Alternative name(s):
    Invertase

Gene names

Name:

scrB

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes sucrose and sucrose-6P, but fails to hydrolyze any of the phosphorylated isomers of sucrose and other phospho-D-glucosides, including maltose-6'P and trehalose-6P.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.
Mass spectrometry	Molecular mass is 52581 Da from positions 2 - 466. Determined by ESI. Ref.2

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-fructofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 466	465	Sucrose-6-phosphate hydrolase		PRO_0000169872
Regions
	Region	38 – 41	4	Substrate binding By similarity
	Region	100 – 101	2	Substrate binding By similarity
	Region	159 – 160	2	Substrate binding By similarity
Sites
	Active site	41	1	By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	218	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27217-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9CE446CE2DB1F075	FASTA	466	52,708
	10 20 30 40 50 60 MSLPSRLPAI LQAVMQGQPQ ALADSHYPQW HLAPVNGLLN DPNGFCQVAG RYHLFYQWNP 70 80 90 100 110 120 LACDHTYKCW GHWSSADLLH WRHEPIALMP DEEYDRNGCY SGSAVEFEGA LTLCYTGNVK 130 140 150 160 170 180 FPDGGRTAWQ CLATENADGT FRKLGPVLPL PEGYTGHVRD PKVWRQDGRW YMVLGAQDVQ 190 200 210 220 230 240 QRGKVLLFTA SDLREWRLVG EIAGHDVNGL ANAGYMWECP DLFPLADTHL LICCPQGLAR 250 260 270 280 290 300 EAQRFLNTYP AVWMAGRFDA ERGIFDHGPL HELDSGFEFY APQTMQADDG RRLLVGWMGV 310 320 330 340 350 360 PDGDEMHQPT RAQGWIHQMT CVRELEWQAG TLYQRPLREL VALRGEAQGW CGQTLPLAPM 370 380 390 400 410 420 ELAFDLSPDS TLGLDFAGAL QLTVNRDGLR LSRRGLQTAE MHHRYWRGEA RRLRIFIDRS 430 440 450 460 SVEIFINDGE GVMSSRFFPG YPGQLIFSGA TPVAFCRWLL RPCMVE

References

[1]	"Molecular analysis of the scrA and scrB genes from Klebsiella pneumoniae and plasmid pUR400, which encode the sucrose transport protein Enzyme II Scr of the phosphotransferase system and a sucrose-6-phosphate invertase." Titgemeyer F., Jahreis K., Ebner R., Lengeler J.W. Mol. Gen. Genet. 250:197-206(1996) [PubMed: 8628219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1033-5P14 / KAY2026.
[2]	"Metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Participation and properties of sucrose-6-phosphate hydrolase and phospho-alpha-glucosidase." Thompson J., Robrish S.A., Immel S., Lichtenthaler F.W., Hall B.G., Pikis A. J. Biol. Chem. 276:37415-37425(2001) [PubMed: 11473129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-29, MASS SPECTROMETRY. Strain: ATCC 23357 / A-11.
[3]	"Molecular analysis of two ScrR repressors and of a ScrR-FruR hybrid repressor for sucrose and D-fructose specific regulons from enteric bacteria." Jahreis K., Lengeler J.W. Mol. Microbiol. 9:195-209(1993) [PubMed: 8412665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 316-466. Strain: 1033-5P14 / KAY2026.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57401 Genomic DNA. Translation: CAA40659.1. X67751 Genomic DNA. Translation: CAA47976.1.
PIR	S62332.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-12621.
BRENDA	3.2.1.26. 758.
Family and domain databases
InterPro	IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR006232. Suc6P_hydrolase. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
TIGRFAMs	TIGR01322. scrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB_KLEPN

Accession

Primary (citable) accession number: P27217

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 59 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)